##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048243_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3009936 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31902771354607 33.0 31.0 34.0 31.0 34.0 2 32.44959128699082 34.0 31.0 34.0 31.0 34.0 3 32.523485881427376 34.0 31.0 34.0 31.0 34.0 4 36.08311704966484 37.0 35.0 37.0 35.0 37.0 5 35.94431509507179 37.0 35.0 37.0 35.0 37.0 6 35.92784929646345 37.0 35.0 37.0 35.0 37.0 7 35.89672737227635 37.0 35.0 37.0 35.0 37.0 8 35.86662939012657 37.0 35.0 37.0 35.0 37.0 9 37.45752966176025 39.0 37.0 39.0 34.0 39.0 10-11 37.45480850755631 39.0 37.0 39.0 34.0 39.0 12-13 37.45907437899012 39.0 37.0 39.0 34.0 39.0 14-15 38.80975326385678 40.0 38.0 41.0 35.0 41.0 16-17 38.82570742367945 40.0 38.0 41.0 35.0 41.0 18-19 38.86852228751708 40.0 38.0 41.0 35.0 41.0 20-21 38.895473192785495 40.0 38.0 41.0 35.0 41.0 22-23 38.88137139793005 40.0 38.0 41.0 35.0 41.0 24-25 38.86847627324966 40.0 38.0 41.0 35.0 41.0 26-27 38.77768995752733 40.0 38.0 41.0 35.0 41.0 28-29 38.6699619194561 40.0 38.0 41.0 34.5 41.0 30-31 38.51087182584613 40.0 38.0 41.0 34.0 41.0 32-33 38.38691703079401 40.0 38.0 41.0 34.0 41.0 34-35 38.33223646615742 40.0 38.0 41.0 34.0 41.0 36-37 38.24188736903376 40.0 37.0 41.0 34.0 41.0 38-39 38.137275842409935 40.0 37.0 41.0 33.5 41.0 40-41 37.96747273031719 40.0 37.0 41.0 33.0 41.0 42-43 37.877939597386785 40.0 37.0 41.0 33.0 41.0 44-45 37.75471372148777 40.0 37.0 41.0 33.0 41.0 46-47 37.6335231048102 40.0 36.5 41.0 33.0 41.0 48-49 37.51640300657556 40.0 36.0 41.0 32.0 41.0 50-51 37.42800378479808 39.5 36.0 41.0 32.0 41.0 52-53 37.40201552458258 39.0 36.0 41.0 32.0 41.0 54-55 37.221318991500155 39.0 35.5 41.0 32.0 41.0 56-57 36.97618221782788 39.0 35.0 41.0 31.0 41.0 58-59 36.73310080347223 39.0 35.0 41.0 31.0 41.0 60-61 36.46551189128274 38.5 35.0 40.0 31.0 41.0 62-63 36.15924723980842 38.0 35.0 40.0 31.0 41.0 64-65 35.84739376518304 37.0 35.0 40.0 30.0 41.0 66-67 35.51811766097352 37.0 34.5 40.0 30.0 41.0 68-69 35.169293134471964 36.0 34.0 39.0 30.0 41.0 70-71 34.81246710893521 36.0 34.0 39.0 29.0 41.0 72-73 34.440912863263534 35.5 34.0 38.5 29.0 40.0 74-75 34.098878182127464 35.0 34.0 37.0 29.0 39.5 76-77 33.85036293130486 35.0 34.0 37.0 29.0 39.0 78-79 33.69695418773024 35.0 34.0 36.5 29.0 39.0 80-81 33.44918496605908 35.0 34.0 36.0 29.0 37.5 82-83 33.21217743500194 35.0 34.0 36.0 29.0 37.0 84-85 33.007214106878024 35.0 34.0 35.0 29.0 37.0 86-87 32.80746500922279 35.0 33.5 35.0 29.0 36.0 88-89 32.670762933165356 35.0 33.0 35.0 29.0 36.0 90-91 32.53807356701272 35.0 33.0 35.0 29.0 36.0 92-93 32.399797537223385 35.0 33.0 35.0 29.0 35.5 94-95 32.282540891234895 35.0 33.0 35.0 29.0 35.0 96-97 32.168346270485486 35.0 33.0 35.0 29.0 35.0 98-99 32.02291444070572 35.0 33.0 35.0 28.5 35.0 100-101 31.565419165058657 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3289411557717088 1101 2 0.3720633925221577 1101 3 0.29139360759772615 1101 4 0.22929293729968947 1101 5 0.27597666850630986 1101 6 0.29687715662657865 1101 7 0.2702055536502712 1101 8 0.31707452187681184 1101 9 0.3995034105647335 1101 10-11 0.3400162873355157 1101 12-13 0.33860051886620823 1101 14-15 0.43639749661630844 1101 16-17 0.42205936472576155 1101 18-19 0.4860803687170403 1101 20-21 0.4352803942473926 1101 22-23 0.4952174088507988 1101 24-25 0.4615727428903398 1101 26-27 0.47717346008421657 1101 28-29 0.5317340168442755 1101 30-31 0.500243459970747 1101 32-33 0.4316391772403847 1101 34-35 0.47581840676433274 1101 36-37 0.47090608878316687 1101 38-39 0.4934062747574899 1101 40-41 0.4675028248828781 1101 42-43 0.4122718338483935 1101 44-45 0.47209264033086384 1101 46-47 0.5298275426826962 1101 48-49 0.4818227458560287 1101 50-51 0.5594512477710296 1101 52-53 0.43913474087791116 1101 54-55 0.5040842782842958 1101 56-57 0.498524118530554 1101 58-59 0.5002198196903436 1101 60-61 0.4011426826407387 1101 62-63 0.39927202184370003 1101 64-65 0.2690900686921651 1101 66-67 0.3585329374066859 1101 68-69 0.26739772530845585 1101 70-71 0.25673927427051524 1101 72-73 0.26771693623772563 1101 74-75 0.2967033644310675 1101 76-77 0.21768617518723232 1101 78-79 0.30324250675891307 1101 80-81 0.26987844165935826 1101 82-83 0.31444539089303447 1101 84-85 0.3141646720422244 1101 86-87 0.3314033215927523 1101 88-89 0.29968451278427466 1101 90-91 0.28093129137421613 1101 92-93 0.30275630285272115 1101 94-95 0.300944137099016 1101 96-97 0.3471742755228604 1101 98-99 0.37933387277332287 1101 100-101 0.3104713580160592 1102 1 0.3618566740608671 1102 2 0.3261987360344918 1102 3 0.32419236005815577 1102 4 0.24474687070559042 1102 5 0.24040758669464424 1102 6 0.31424470417369577 1102 7 0.27628631437532647 1102 8 0.31097497290524956 1102 9 0.3186993428084506 1102 10-11 0.4002865795667532 1102 12-13 0.34506948993293207 1102 14-15 0.46012839741470657 1102 16-17 0.46155728031155974 1102 18-19 0.45917146725427926 1102 20-21 0.4936320028744774 1102 22-23 0.49898988212836315 1102 24-25 0.5028807931061863 1102 26-27 0.5247465976584351 1102 28-29 0.5744224505834836 1102 30-31 0.5508566415450176 1102 32-33 0.5328565787317103 1102 34-35 0.4749939102737173 1102 36-37 0.46743619757288 1102 38-39 0.4412114928253672 1102 40-41 0.5078655764297508 1102 42-43 0.42971780190270437 1102 44-45 0.4907173666339233 1102 46-47 0.44569069947426243 1102 48-49 0.47289028008800216 1102 50-51 0.474502065172679 1102 52-53 0.49153496820127174 1102 54-55 0.42020931728432487 1102 56-57 0.5262759443420748 1102 58-59 0.39663959197967813 1102 60-61 0.47874832772556886 1102 62-63 0.5286224439281426 1102 64-65 0.4564106643631902 1102 66-67 0.4770912897841484 1102 68-69 0.41142611660737316 1102 70-71 0.3972235824087065 1102 72-73 0.4346863771787923 1102 74-75 0.412548112585192 1102 76-77 0.37621452787654874 1102 78-79 0.41370235605987915 1102 80-81 0.34994700482413066 1102 82-83 0.3233276906013174 1102 84-85 0.2659303900081653 1102 86-87 0.3003813798519772 1102 88-89 0.2712010986014235 1102 90-91 0.23539321881862207 1102 92-93 0.2674122715590954 1102 94-95 0.24961505606002277 1102 96-97 0.29083798263225447 1102 98-99 0.34205258586204224 1102 100-101 0.31341911856999616 1103 1 0.2958942274592147 1103 2 0.30063353901677203 1103 3 0.26450689771064617 1103 4 0.20510251359572607 1103 5 0.20088448564457195 1103 6 0.2050614133153985 1103 7 0.22704898807710805 1103 8 0.2137968818920939 1103 9 0.2753310364510497 1103 10-11 0.28255452401164405 1103 12-13 0.29813262906817073 1103 14-15 0.4102947066168099 1103 16-17 0.354510915025692 1103 18-19 0.40933749314783086 1103 20-21 0.3811836517939895 1103 22-23 0.43017713902306554 1103 24-25 0.4693459848968615 1103 26-27 0.44106511352959643 1103 28-29 0.4112837544742618 1103 30-31 0.4779731086987198 1103 32-33 0.44588744052373386 1103 34-35 0.40687753958658135 1103 36-37 0.407180576766649 1103 38-39 0.44794538294499375 1103 40-41 0.4200108156916116 1103 42-43 0.38439091270566905 1103 44-45 0.4125277479374958 1103 46-47 0.4459039599859693 1103 48-49 0.4226859508783676 1103 50-51 0.40946724159386605 1103 52-53 0.3445745360650747 1103 54-55 0.2792152215761803 1103 56-57 0.2306623114779427 1103 58-59 0.26978726755342564 1103 60-61 0.25050157277712515 1103 62-63 0.21094438698284534 1103 64-65 0.15283259068851862 1103 66-67 0.06358985248903792 1103 68-69 0.045067505493548765 1103 70-71 0.061555786615812735 1103 72-73 0.09231677595965948 1103 74-75 0.14792517714314357 1103 76-77 0.09623626897474225 1103 78-79 0.017825331235954422 1103 80-81 0.05674992307218929 1103 82-83 0.005141944732770298 1103 84-85 0.04248543168922225 1103 86-87 0.06043242355359979 1103 88-89 0.064335852472162 1103 90-91 0.12351141401283172 1103 92-93 0.11861805399994552 1103 94-95 0.07782985380391949 1103 96-97 0.09040327399172554 1103 98-99 0.19609520765036592 1103 100-101 0.2578521834543146 1104 1 0.2645244441054686 1104 2 0.23037504964131017 1104 3 0.20213874592123204 1104 4 0.1375873587698706 1104 5 0.1535694933448397 1104 6 0.2174010728416107 1104 7 0.18161184719226497 1104 8 0.22746121571822897 1104 9 0.3007528585131922 1104 10-11 0.27103349471374827 1104 12-13 0.30714167025507066 1104 14-15 0.3895540198283669 1104 16-17 0.3817495886799094 1104 18-19 0.41036400834444464 1104 20-21 0.3636599177737665 1104 22-23 0.36451590724685445 1104 24-25 0.393160567846472 1104 26-27 0.4220617894711296 1104 28-29 0.4145242154022739 1104 30-31 0.4148868958492642 1104 32-33 0.3719934737633892 1104 34-35 0.2820696376699985 1104 36-37 0.37107678228819196 1104 38-39 0.2710954576831952 1104 40-41 0.33997762413093113 1104 42-43 0.32855623179683846 1104 44-45 0.3448414994639464 1104 46-47 0.4055655218569001 1104 48-49 0.30400387387758343 1104 50-51 0.37874163317724907 1104 52-53 0.40033778204936965 1104 54-55 0.4316022569311002 1104 56-57 0.43096590990721495 1104 58-59 0.39610573798695015 1104 60-61 0.3955503738026138 1104 62-63 0.38781030339603717 1104 64-65 0.3432122206229238 1104 66-67 0.3083068546313896 1104 68-69 0.2770458163563916 1104 70-71 0.26022156431453425 1104 72-73 0.18124009056817414 1104 74-75 0.17508643961769366 1104 76-77 0.18902463320601726 1104 78-79 0.14772668334033057 1104 80-81 0.1988831286630699 1104 82-83 0.14272689334747923 1104 84-85 0.16229632056612786 1104 86-87 0.20673180265337265 1104 88-89 0.22681054968496284 1104 90-91 0.20802658681414243 1104 92-93 0.20058190279228683 1104 94-95 0.24784156586327555 1104 96-97 0.25247378026224254 1104 98-99 0.2893496497471091 1104 100-101 0.2711347606396366 1105 1 0.13904897608748712 1105 2 0.2115581994623028 1105 3 0.15299266898205133 1105 4 0.09220260020452997 1105 5 0.14681065971002027 1105 6 0.14087032181728887 1105 7 0.11251242931653138 1105 8 0.12539488576550184 1105 9 0.18482091427569003 1105 10-11 0.15537362777470776 1105 12-13 0.21396456030409894 1105 14-15 0.2618839233584822 1105 16-17 0.16967996771285954 1105 18-19 0.2373169746902235 1105 20-21 0.21377034546173235 1105 22-23 0.1975692490653458 1105 24-25 0.24607344044708412 1105 26-27 0.29115705296347727 1105 28-29 0.1950388112132586 1105 30-31 0.20659943479950016 1105 32-33 0.16119061480493713 1105 34-35 0.2161622019735887 1105 36-37 0.2514817627208146 1105 38-39 0.1940952613363649 1105 40-41 0.21743958367233773 1105 42-43 0.12683817378308504 1105 44-45 0.12065897303255468 1105 46-47 0.09892665474564666 1105 48-49 0.22278669988875777 1105 50-51 0.2694699546067625 1105 52-53 0.16481302308483947 1105 54-55 0.24231810592692682 1105 56-57 0.18535611837371135 1105 58-59 0.20538470413499255 1105 60-61 0.11957099532622095 1105 62-63 0.05712960187618421 1105 64-65 0.01327865826470287 1105 66-67 0.07667347641214661 1105 68-69 0.048834721692621486 1105 70-71 0.10800182389154145 1105 72-73 0.07102411369160677 1105 74-75 0.06542847301893318 1105 76-77 0.12450056075913807 1105 78-79 0.03536634837170283 1105 80-81 0.02338828125890302 1105 82-83 0.03761484336119736 1105 84-85 0.061960627821541436 1105 86-87 0.07889332600773713 1105 88-89 -0.024538255593292035 1105 90-91 0.02415615437300289 1105 92-93 -0.015948086981900644 1105 94-95 -0.1272723779648146 1105 96-97 -0.031717235964919155 1105 98-99 -0.08717847867764661 1105 100-101 -0.08929710491987919 1106 1 0.0904111221441184 1106 2 0.14667254289179255 1106 3 0.08984153511628534 1106 4 0.08999260726177027 1106 5 0.0841856128565368 1106 6 0.0907600336418426 1106 7 0.08920752050813263 1106 8 0.14278436269730577 1106 9 0.23899573397218887 1106 10-11 0.17822077994219399 1106 12-13 0.1984521789317668 1106 14-15 0.2035732174968814 1106 16-17 0.257200054113369 1106 18-19 0.19550112952062193 1106 20-21 0.2222651635865205 1106 22-23 0.25887712284338704 1106 24-25 0.2650606883853257 1106 26-27 0.2432590088811324 1106 28-29 0.24151783472154875 1106 30-31 0.23831400458102792 1106 32-33 0.2559556078982297 1106 34-35 0.2698857683523741 1106 36-37 0.26282485449525694 1106 38-39 0.24285197146978277 1106 40-41 0.223179403083698 1106 42-43 0.15715061798752572 1106 44-45 0.269821808878028 1106 46-47 0.18994923546620868 1106 48-49 0.18984122838919149 1106 50-51 0.179069964757403 1106 52-53 0.11520890265202155 1106 54-55 0.17530881872662007 1106 56-57 0.21271082390095586 1106 58-59 0.1774104320092107 1106 60-61 0.16070740239386794 1106 62-63 0.2034284969752207 1106 64-65 0.17270144148874778 1106 66-67 0.301777140748257 1106 68-69 0.21768623208716065 1106 70-71 0.22532943739295774 1106 72-73 0.16516280947424633 1106 74-75 0.14120239804008605 1106 76-77 0.25973648449799214 1106 78-79 0.2757501390341801 1106 80-81 0.2009993132235124 1106 82-83 0.20776531297845224 1106 84-85 0.2289117580308968 1106 86-87 0.19952494603830218 1106 88-89 0.20990793277260167 1106 90-91 0.2556037195333545 1106 92-93 0.27607968466396215 1106 94-95 0.28812038773845927 1106 96-97 0.2903193701953768 1106 98-99 0.3431025085431898 1106 100-101 0.2813064325786918 1107 1 0.044645988434510286 1107 2 0.14229242634994677 1107 3 0.09571151611284989 1107 4 0.04356546382142312 1107 5 0.06966236902529488 1107 6 0.1110891311299298 1107 7 0.09044548875304059 1107 8 0.07336363993833572 1107 9 0.17114530889105595 1107 10-11 0.09856790981896779 1107 12-13 0.10534754878253949 1107 14-15 0.09716970068780029 1107 16-17 0.10337829760707251 1107 18-19 0.05379382222852769 1107 20-21 0.08940478004108598 1107 22-23 0.1815498051173563 1107 24-25 0.18321709770739858 1107 26-27 0.1086708183052707 1107 28-29 0.0758562940704337 1107 30-31 0.10976081784961167 1107 32-33 0.10790268566925221 1107 34-35 0.04499036579847626 1107 36-37 0.07011452527079598 1107 38-39 0.07026867466863251 1107 40-41 0.15469492121478368 1107 42-43 0.1010815818917763 1107 44-45 0.1538193793470768 1107 46-47 0.09226790467958068 1107 48-49 0.10552581194834687 1107 50-51 0.20755883231517203 1107 52-53 0.23938921228840826 1107 54-55 0.07868828385527138 1107 56-57 0.18822843064671702 1107 58-59 0.11473061625381575 1107 60-61 0.10227890040395948 1107 62-63 -0.22594709091582388 1107 64-65 0.0064628461296152295 1107 66-67 -0.05138879038756983 1107 68-69 -0.03969496630666214 1107 70-71 0.003713823975211028 1107 72-73 -0.019245769740692253 1107 74-75 0.04155527197257669 1107 76-77 0.025732190706712288 1107 78-79 -0.04139152821635861 1107 80-81 -0.0081179775991842 1107 82-83 0.03371985345444273 1107 84-85 0.029968242250404842 1107 86-87 -0.03236988256676909 1107 88-89 -0.019381539957244343 1107 90-91 0.005469549974634447 1107 92-93 0.015941535921598415 1107 94-95 0.0506467774633208 1107 96-97 0.011952088804520145 1107 98-99 -0.13424471423601148 1107 100-101 -0.14540117936394026 1108 1 -0.027858538034230662 1108 2 0.012029225472460325 1108 3 0.03361610628659406 1108 4 0.01083346965973675 1108 5 -0.041275896318964556 1108 6 -0.03216679875686168 1108 7 0.005930847810191153 1108 8 -0.0746196097253673 1108 9 -0.21302796763544052 1108 10-11 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78-79 -0.09183153840256608 2214 80-81 -0.1384846236256223 2214 82-83 -0.13876619380347677 2214 84-85 -0.19337024610713627 2214 86-87 -0.1374622388166742 2214 88-89 -0.08345757438357282 2214 90-91 -0.06558079665582994 2214 92-93 -0.08448597483307907 2214 94-95 -0.09893319222509689 2214 96-97 -0.07157746699807888 2214 98-99 -0.226535881996778 2214 100-101 -0.3764731190826396 2215 1 -0.5743574221560834 2215 2 -0.9256531916427093 2215 3 -0.5549239553309597 2215 4 -0.33038084852515226 2215 5 -0.2996747833165827 2215 6 -0.3229490129628303 2215 7 -0.3160180276344775 2215 8 -0.4228489327768301 2215 9 -0.3703199303644453 2215 10-11 -0.47411782660064716 2215 12-13 -0.376612219600041 2215 14-15 -0.5043998292621197 2215 16-17 -0.4898990119890527 2215 18-19 -0.4719981996188949 2215 20-21 -0.5260707491899339 2215 22-23 -0.5292496019110899 2215 24-25 -0.4989307264116789 2215 26-27 -0.5756910724870181 2215 28-29 -0.4920833733797849 2215 30-31 -0.5548430870822898 2215 32-33 -0.5694698281429851 2215 34-35 -0.5807524525011516 2215 36-37 -0.5694598640408373 2215 38-39 -0.6051037130664767 2215 40-41 -0.6481132606323072 2215 42-43 -0.5977337580859512 2215 44-45 -0.5563772563277993 2215 46-47 -0.6014275513932361 2215 48-49 -0.6129774090616849 2215 50-51 -0.5151145102402594 2215 52-53 -0.5321369214712277 2215 54-55 -0.6598625470728905 2215 56-57 -0.42590715729755146 2215 58-59 -0.4043961937105891 2215 60-61 -0.4126628186817243 2215 62-63 -0.4222074167340537 2215 64-65 -0.30832387062326916 2215 66-67 -0.3492836837250266 2215 68-69 -0.30049329479929554 2215 70-71 -0.24116048104326637 2215 72-73 -0.18920209461873583 2215 74-75 -0.2842395298938811 2215 76-77 -0.31106999894414145 2215 78-79 -0.28838464474043946 2215 80-81 -0.23820555656706688 2215 82-83 -0.35665586007603167 2215 84-85 -0.3042392784542258 2215 86-87 -0.29759583303110304 2215 88-89 -0.2959667040276486 2215 90-91 -0.2808471219018571 2215 92-93 -0.31055226872693 2215 94-95 -0.3526117209805193 2215 96-97 -0.4189359868848932 2215 98-99 -0.5035906994143282 2215 100-101 -0.43116300964665655 2216 1 -0.4911981705477899 2216 2 -0.45978165584753583 2216 3 -0.43752733094391516 2216 4 -0.32383454026781777 2216 5 -0.36582927869764603 2216 6 -0.30539952958751115 2216 7 -0.3808576040151337 2216 8 -0.44342307076822607 2216 9 -0.5581255792365738 2216 10-11 -0.6452461820811806 2216 12-13 -0.6822258484668922 2216 14-15 -0.9150441220658649 2216 16-17 -1.2091399413422153 2216 18-19 -1.0950197399288868 2216 20-21 -1.4814866933314477 2216 22-23 -1.3076183965640595 2216 24-25 -1.3091738449930261 2216 26-27 -1.1494881026552548 2216 28-29 -1.2384003839407853 2216 30-31 -1.243986229067005 2216 32-33 -0.9563003675076232 2216 34-35 -0.594917477553814 2216 36-37 -0.8825183890391344 2216 38-39 -1.15232870970339 2216 40-41 -1.1711054085268344 2216 42-43 -0.6027887928500846 2216 44-45 -1.1459500896276538 2216 46-47 -0.7438254657295502 2216 48-49 -0.7375111845404874 2216 50-51 -0.7803162859475705 2216 52-53 -0.8162024545796172 2216 54-55 -1.1125731451117957 2216 56-57 -1.2551140043482718 2216 58-59 -0.9001400521699807 2216 60-61 -1.0163323557926418 2216 62-63 -0.861792504551957 2216 64-65 -0.9791520364812527 2216 66-67 -0.729500192495756 2216 68-69 -0.7539736239943409 2216 70-71 -0.6642595104485878 2216 72-73 -0.6866254670252516 2216 74-75 -0.6726785899262779 2216 76-77 -0.622565197568683 2216 78-79 -0.5351240676171329 2216 80-81 -0.557932428237379 2216 82-83 -0.764212244679932 2216 84-85 -0.7099031232033042 2216 86-87 -0.7407322433234285 2216 88-89 -0.7151165998181739 2216 90-91 -0.645763542403202 2216 92-93 -0.643854290077428 2216 94-95 -0.711380473618 2216 96-97 -0.7639986599914792 2216 98-99 -1.022910868508248 2216 100-101 -0.7590079466879196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 23.0 13 104.0 14 390.0 15 958.0 16 1787.0 17 3269.0 18 6129.0 19 12928.0 20 14677.0 21 12877.0 22 12007.0 23 12137.0 24 13022.0 25 15236.0 26 19119.0 27 24628.0 28 31551.0 29 40446.0 30 52009.0 31 67773.0 32 87924.0 33 117393.0 34 160957.0 35 231823.0 36 381447.0 37 704679.0 38 812221.0 39 172190.0 40 229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.928491502809365 39.829052843648505 13.551450927860259 20.691004725681875 2 24.40151551395113 27.85109052152604 30.09549040245374 17.651903562069094 3 22.811714269007712 27.498092982707938 24.271878206048232 25.418314542236114 4 24.15338820868943 26.158301864091428 26.03632462298397 23.651985304235172 5 23.86183626495713 27.016654174706705 24.73800107377698 24.383508486559183 6 23.945027402576002 25.470740906118934 26.0895912737015 24.494640417603563 7 24.81022852313139 26.75259540402188 24.26127997405925 24.175896098787483 8 25.21694813444538 25.291401544750453 25.770082819036684 23.721567501767478 9 23.629970869812514 25.609282057824483 26.143246899601852 24.617500172761147 10-11 25.15123245145412 25.541440083775868 25.160219353501205 24.14710811126881 12-13 24.59490833027679 25.66659224647966 25.218443182845085 24.520056240398468 14-15 24.207873522892182 25.71857009584257 25.359941208052266 24.713615173212986 16-17 24.3379427336661 26.082232313245196 25.300986466157422 24.278838486931285 18-19 23.650540019877504 25.962881928865254 26.366761015310793 24.01981703594645 20-21 22.982448796253475 26.626944891851522 26.386707225668587 24.00389908622642 22-23 22.55067881842006 26.170107935849803 27.54326005602777 23.73595318970237 24-25 22.508551676846285 25.55570949016856 28.780279713588598 23.15545911939656 26-27 22.429089000260802 25.402209682268882 29.528793811162036 22.639907506308276 28-29 22.52437015998041 25.45777627460563 29.51866670697767 22.49918685843629 30-31 22.52109347175488 25.432367997193296 29.4892648880242 22.557273643027624 32-33 22.456108701314577 25.469013294634834 29.548203018270158 22.526674985780428 34-35 22.577406961476925 25.614714067010063 29.37258466625204 22.435294305260975 36-37 22.601244677627697 25.68546307961365 29.14987228964337 22.563419953115282 38-39 22.62846959434991 25.675197529248152 29.14236657680866 22.55396629959328 40-41 22.811072538823954 25.943999417927245 28.482394790847504 22.762533252401298 42-43 22.86063795097134 26.10335078831476 28.223879364776668 22.812131895937235 44-45 22.894134274397445 26.106754784004654 28.185028469138445 22.814082472459457 46-47 22.96074364445986 26.415089638207895 27.912182762872128 22.711983954460116 48-49 22.967485515885638 26.443639739123974 27.71207223543495 22.87680250955544 50-51 22.961788825059447 26.402349422627964 27.719223463926586 22.91663828838601 52-53 22.989291470902344 26.539570127218916 27.545752910280292 22.925385491598448 54-55 23.06268673774537 26.537316983304777 27.450419498568557 22.949576780381296 56-57 23.087431875537387 26.487658192942565 27.45635117338199 22.96855875813806 58-59 23.19255683160343 26.656225570209784 27.20611076576937 22.945106832417412 60-61 23.089493046495242 26.644054777517418 27.170331048555756 23.096121127431584 62-63 23.138516740450342 26.555894249856728 27.25290497271527 23.052684036977666 64-65 23.186912911718668 26.735601858981035 27.05587524753495 23.021609981765348 66-67 23.233030823328665 26.67719958749844 26.948900438814483 23.140869150358416 68-69 23.26088285461597 26.774666286589866 26.79174316376725 23.172707695026908 70-71 23.255789229054642 26.79727693895007 26.816928614251236 23.130005217744053 72-73 23.259825089217056 26.77285834625305 26.642872128945 23.324444435584894 74-75 23.300535580531186 26.79160167998295 26.69379149519845 23.214071244287414 76-77 23.343920582342996 26.915140319810227 26.571060264260392 23.169878833586385 78-79 23.334132907897647 26.811783912869853 26.480829812215152 23.373253367017348 80-81 23.226577446593126 26.89183871215845 26.53221306569526 23.34937077555316 82-83 23.317844307716637 26.98055698695593 26.40960266429846 23.291996041028973 84-85 23.296425571839404 26.872697625464458 26.548089394591777 23.282787408104358 86-87 23.30709412211657 26.92999567598708 26.508740801920837 23.254169399975513 88-89 23.345638192617617 27.001580107391487 26.375284808044857 23.277496891946036 90-91 23.377100153707968 26.990973373620005 26.312601777749872 23.319324694922155 92-93 23.330971147444256 26.966893516248252 26.377426805460868 23.324708530846628 94-95 23.393387766384404 27.044511909887785 26.289047341870393 23.273052981857422 96-97 23.360210556986285 27.03342927714228 26.262362656301836 23.343997509569604 98-99 23.40590022610119 26.968833717533148 26.31018172980783 23.315084326557827 100-101 23.430079418676222 27.095565728278647 26.118053980777038 23.356300872268086 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2433.0 1 1721.0 2 743.5 3 374.5 4 251.0 5 219.0 6 192.5 7 176.5 8 184.0 9 181.0 10 174.5 11 182.5 12 221.0 13 360.5 14 455.0 15 479.5 16 2923.0 17 3327.5 18 1412.5 19 1611.0 20 2013.5 21 2713.5 22 3609.5 23 4689.0 24 6014.5 25 7607.5 26 9722.0 27 12383.5 28 15205.5 29 18608.5 30 23373.0 31 28828.0 32 34858.0 33 42881.5 34 50942.0 35 58659.0 36 66507.5 37 73117.5 38 78596.0 39 84549.0 40 89544.0 41 92392.0 42 96338.5 43 104529.5 44 114892.5 45 122148.5 46 125335.5 47 125879.5 48 124880.0 49 124055.0 50 123165.5 51 122146.5 52 122405.5 53 121194.5 54 113367.0 55 103510.0 56 94549.0 57 81886.5 58 70438.5 59 61019.5 60 50610.5 61 41425.0 62 33307.5 63 25961.0 64 19930.5 65 14860.5 66 10734.0 67 7791.5 68 5945.5 69 4726.0 70 3977.5 71 3453.0 72 3157.5 73 2584.0 74 1924.0 75 1555.5 76 1149.5 77 805.0 78 539.5 79 298.5 80 116.0 81 61.5 82 42.0 83 14.5 84 2.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.03913704477437394 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.66116488855577E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.32232977711154E-5 28-29 1.66116488855577E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.64465955422308E-5 40-41 9.966989331334621E-5 42-43 8.30582444277885E-5 44-45 3.32232977711154E-4 46-47 9.966989331334621E-5 48-49 1.66116488855577E-5 50-51 1.66116488855577E-4 52-53 0.0015116600485857506 54-55 0.001096368826446808 56-57 1.328931910844616E-4 58-59 0.001162815421989039 60-61 4.983494665667309E-4 62-63 3.654562754822694E-4 64-65 2.4917473328336546E-4 66-67 2.657863821689232E-4 68-69 5.980193598800772E-4 70-71 4.817378176811733E-4 72-73 4.9834946656673106E-5 74-75 8.139707953923272E-4 76-77 8.638057420490003E-4 78-79 6.64465955422308E-5 80-81 1.66116488855577E-5 82-83 0.00209306775958027 84-85 0.0 86-87 1.66116488855577E-5 88-89 4.6512616879561557E-4 90-91 2.4917473328336546E-4 92-93 3.9867957325338485E-4 94-95 0.0 96-97 4.817378176811733E-4 98-99 1.66116488855577E-5 100-101 0.008422105984977753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3009936.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.13268509749588 #Duplication Level Percentage of deduplicated Percentage of total 1 87.51159064393329 56.998648757922744 2 7.665195702085627 9.98509555749244 3 1.7502589678462799 3.4199719857539974 4 0.7249605941817573 1.888745203557356 5 0.40775658660620934 1.327914067592602 6 0.2658255996025021 1.0388361041857677 7 0.19130132361272748 0.872197820872135 8 0.15259992511940035 0.7951394294962684 9 0.12072490019095926 0.7076823216807933 >10 1.071722934466516 14.535474209267235 >50 0.11988411355133509 5.2106625183396105 >100 0.017564465889634236 1.541659590878302 >500 1.535607284711658E-4 0.06921261218752302 >1k 3.5830836635695524E-4 0.5372645689841293 >5k 0.0 0.0 >10k+ 1.0237381895913008E-4 1.0714952517890741 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 18725 0.6221062507641358 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13414 0.445657316301742 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 4270 0.14186348148266276 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3340 0.11096581455552544 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3077 0.1022280872417221 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 3.32232977711154E-5 0.0 0.0 0.0 2 0.0 6.64465955422308E-5 0.0 0.0 0.0 3 0.0 6.64465955422308E-5 0.0 0.0 0.0 4 3.32232977711154E-5 6.64465955422308E-5 0.0 0.0 0.0 5 3.32232977711154E-5 6.64465955422308E-5 0.0 3.32232977711154E-5 0.0 6 6.64465955422308E-5 6.64465955422308E-5 0.0 3.32232977711154E-5 0.0 7 1.328931910844616E-4 6.64465955422308E-5 0.0 3.32232977711154E-5 0.0 8 1.6611648885557698E-4 6.64465955422308E-5 0.0 6.64465955422308E-5 0.0 9 3.3223297771115397E-4 6.64465955422308E-5 0.0 6.64465955422308E-5 0.0 10-11 6.810776043078657E-4 9.96698933133462E-5 0.0 6.64465955422308E-5 0.0 12-13 0.0011462037731034811 9.96698933133462E-5 0.0 6.64465955422308E-5 0.0 14-15 0.0015116600485857506 9.96698933133462E-5 0.0 6.64465955422308E-5 0.0 16-17 0.0018605046751824623 9.96698933133462E-5 0.0 6.64465955422308E-5 0.0 18-19 0.0021429027062369434 9.96698933133462E-5 0.0 8.30582444277885E-5 0.0 20-21 0.002441912386176982 9.96698933133462E-5 0.0 9.96698933133462E-5 0.0 22-23 0.002923650203858155 9.96698933133462E-5 0.0 9.96698933133462E-5 0.0 24-25 0.003804067594792713 9.96698933133462E-5 0.0 9.96698933133462E-5 3.32232977711154E-5 26-27 0.0052991159944929065 9.96698933133462E-5 0.0 9.96698933133462E-5 3.32232977711154E-5 28-29 0.007674581785127657 9.96698933133462E-5 0.0 9.96698933133462E-5 3.32232977711154E-5 30-31 0.010631455286756929 1.328931910844616E-4 0.0 1.1628154219890389E-4 3.32232977711154E-5 32-33 0.013953785063868468 1.328931910844616E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 34-35 0.018123308934143448 1.328931910844616E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 36-37 0.024103502532944222 1.328931910844616E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 38-39 0.032592055113464205 1.6611648885557698E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 40-41 0.04378830646233009 1.6611648885557698E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 42-43 0.0574929167929152 1.6611648885557698E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 44-45 0.0752839927493475 1.6611648885557698E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 46-47 0.09606516550518018 1.6611648885557698E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 48-49 0.12011883309146773 1.6611648885557698E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 50-51 0.15113278156080395 1.6611648885557698E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 52-53 0.18691427326029525 1.993397866266924E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 54-55 0.22952315265175074 1.993397866266924E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 56-57 0.2781786722375492 1.993397866266924E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 58-59 0.3336117445686553 1.993397866266924E-4 0.0 1.328931910844616E-4 3.32232977711154E-5 60-61 0.39474261246750764 2.159514355122501E-4 0.0 1.328931910844616E-4 4.98349466566731E-5 62-63 0.46421252810690994 2.3256308439780778E-4 0.0 1.328931910844616E-4 6.64465955422308E-5 64-65 0.5379516375099005 2.3256308439780778E-4 0.0 1.328931910844616E-4 6.64465955422308E-5 66-67 0.6244484932569996 2.3256308439780778E-4 0.0 1.328931910844616E-4 6.64465955422308E-5 68-69 0.719035222011365 2.3256308439780778E-4 0.0 1.328931910844616E-4 6.64465955422308E-5 70-71 0.822691911057245 2.657863821689232E-4 0.0 1.4950483997001928E-4 6.64465955422308E-5 72-73 0.9355016186390674 2.990096799400386E-4 0.0 1.993397866266924E-4 6.64465955422308E-5 74-75 1.0580623641167122 2.990096799400386E-4 0.0 1.993397866266924E-4 6.64465955422308E-5 76-77 1.192218040516476 2.990096799400386E-4 0.0 2.4917473328336546E-4 6.64465955422308E-5 78-79 1.3352277257722425 2.990096799400386E-4 0.0 2.657863821689232E-4 6.64465955422308E-5 80-81 1.4907625942877192 2.990096799400386E-4 0.0 2.657863821689232E-4 6.64465955422308E-5 82-83 1.6616798496712222 2.990096799400386E-4 0.0 2.657863821689232E-4 6.64465955422308E-5 84-85 1.8467170066074494 2.990096799400386E-4 0.0 2.8239803105448093E-4 6.64465955422308E-5 86-87 2.0439138905279046 2.990096799400386E-4 0.0 2.990096799400386E-4 6.64465955422308E-5 88-89 2.2507123075042124 2.990096799400386E-4 0.0 2.990096799400386E-4 6.64465955422308E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4230 0.0 69.42546 1 TATCTCG 4365 0.0 67.8226 2 TCGACGG 4490 0.0 65.61695 2 ATATCTC 4645 0.0 63.836567 1 CGACGGC 4970 0.0 59.279697 3 GACGGCG 6025 0.0 49.37282 4 ACGGCGC 6135 0.0 48.77632 5 CGGCGCG 6210 0.0 47.881283 6 GGTCGAC 300 0.0 47.51937 1 ATCTCGA 6820 0.0 43.478134 3 TCTCGAG 6530 0.0 42.27987 4 CTCGAGG 7140 0.0 38.51797 5 TCGAGGG 7370 0.0 37.444817 6 CGAGGGC 7530 0.0 36.523018 7 GAGGGCG 7940 0.0 34.577244 8 AGGGCGC 8015 0.0 34.312954 9 GGGCGCG 7960 0.0 26.195972 8 CTCGACG 860 0.0 25.417337 3 CGCGCCG 14070 0.0 24.711554 9 GGCGCGC 14555 0.0 23.98602 7 >>END_MODULE