FastQCFastQC Report
Tue 31 May 2016
SRR2048242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449111
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT272941.1144452007279375No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG40290.16450867273880196No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT28940.1181653261122097No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA25590.10448689340744459No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA25550.10432356883783546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC181050.092.39281
TATCTCG186200.089.607792
GTCGACG140050.087.495131
ATCTCGA193500.086.374513
TCTCGAG156500.085.066014
CTCGAGG158900.083.542075
CTCGACG39800.083.1758045
CGAGGGC160350.082.697767
TCGAGGG161450.082.163746
AGGGCGC164100.080.899749
GAGGGCG165000.080.2569668
TCTCGAC42300.078.14774
TCGACGG173950.070.416472
CGACGGC178050.068.7682953
CGGCGCG189150.064.582066
GACGGCG190050.064.276234
ACGGCGC191150.063.8318025
GGGCGCG163800.040.52395610-11
GATCCTT131800.039.2704220-21
GGATCCT138650.038.2043818-19
Produced by FastQC (version 0.11.5)