##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048242_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2449111 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32919986068414 31.0 31.0 34.0 28.0 34.0 2 31.475689341969392 31.0 31.0 34.0 28.0 34.0 3 31.507569889645673 31.0 31.0 34.0 28.0 34.0 4 35.151685652467364 37.0 35.0 37.0 32.0 37.0 5 35.02982959939341 37.0 35.0 37.0 32.0 37.0 6 35.04376812647528 37.0 35.0 37.0 32.0 37.0 7 34.93350076823794 37.0 35.0 37.0 32.0 37.0 8 34.99755503119295 37.0 35.0 37.0 32.0 37.0 9 36.46049689050435 39.0 37.0 39.0 32.0 39.0 10-11 36.460388279665565 39.0 37.0 39.0 32.0 39.0 12-13 36.495395472071294 39.0 37.0 39.0 32.0 39.0 14-15 37.6662780902948 40.0 37.0 41.0 32.0 41.0 16-17 37.55073391936911 40.0 37.0 41.0 31.5 41.0 18-19 37.56343607945904 40.0 37.0 41.0 31.5 41.0 20-21 37.56451075512706 40.0 37.0 41.0 31.5 41.0 22-23 37.48289930509479 40.0 37.0 41.0 31.0 41.0 24-25 37.58513579008873 40.0 37.0 41.0 31.5 41.0 26-27 37.546612832166446 40.0 37.0 41.0 31.0 41.0 28-29 37.35330452560133 39.5 37.0 41.0 31.0 41.0 30-31 37.034139122318265 39.0 35.5 41.0 31.0 41.0 32-33 36.818801189492845 39.0 35.0 41.0 31.0 41.0 34-35 36.54407885146896 38.5 35.0 40.0 30.0 41.0 36-37 36.31485138893255 38.0 35.0 40.0 30.0 41.0 38-39 36.044228701761575 38.0 35.0 40.0 29.5 41.0 40-41 35.43796463288108 38.0 34.0 40.0 25.5 41.0 42-43 35.21274474697145 38.0 33.5 40.0 25.0 41.0 44-45 35.00655299004414 38.0 33.0 40.0 24.0 41.0 46-47 34.88166930776106 38.0 33.0 40.0 24.0 41.0 48-49 34.79455157402012 38.0 33.0 40.0 23.5 41.0 50-51 34.87748513644338 38.0 33.0 40.0 24.0 41.0 52-53 34.71635585320551 37.0 33.0 40.0 24.0 41.0 54-55 34.32515696511918 37.0 32.0 40.0 24.5 41.0 56-57 34.02469794141629 37.0 32.0 40.0 24.0 41.0 58-59 33.71642363290189 36.0 32.0 40.0 23.5 41.0 60-61 33.44929752061054 36.0 32.0 39.5 23.0 41.0 62-63 33.284607965910894 35.5 32.0 39.0 23.0 41.0 64-65 32.99399108492837 35.0 31.0 39.0 23.0 41.0 66-67 32.73386812602614 35.0 31.0 39.0 22.5 40.5 68-69 32.55483438684486 35.0 31.0 38.0 23.0 40.0 70-71 32.24945643541677 35.0 31.0 37.0 22.0 40.0 72-73 31.948625235850887 35.0 31.0 37.0 21.5 39.5 74-75 31.661431637847365 35.0 31.0 36.0 20.5 39.0 76-77 31.366885576031464 35.0 31.0 36.0 20.0 39.0 78-79 31.0960658785984 35.0 31.0 35.5 19.5 37.5 80-81 30.830819427947528 34.5 31.0 35.0 19.0 37.0 82-83 30.600550567124152 34.0 31.0 35.0 18.5 37.0 84-85 30.386323241372075 34.0 30.0 35.0 18.0 36.0 86-87 30.18677859027214 34.0 30.0 35.0 17.0 36.0 88-89 29.986428136576905 34.0 30.0 35.0 13.5 36.0 90-91 29.798878858491918 34.0 30.0 35.0 8.5 35.5 92-93 29.60110464572655 34.0 30.0 35.0 4.5 35.0 94-95 29.407340255300802 34.0 30.0 35.0 2.0 35.0 96-97 29.201691960878865 34.0 30.0 35.0 2.0 35.0 98-99 28.91917965335177 34.0 30.0 35.0 2.0 35.0 100-101 28.124595006106297 33.5 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8403680001250713 1101 2 0.9316118620722946 1101 3 0.8821314694038307 1101 4 0.7558724734520013 1101 5 0.8001100214985684 1101 6 0.7096254970335281 1101 7 0.6879092176604047 1101 8 0.705269539679179 1101 9 0.9062110287808665 1101 10-11 0.9426846771451167 1101 12-13 0.8169207656424717 1101 14-15 1.019950269919164 1101 16-17 1.0862110994800531 1101 18-19 1.0656022840609154 1101 20-21 1.1574861419805202 1101 22-23 1.1779731272945781 1101 24-25 1.0174459322243976 1101 26-27 1.0420602350331336 1101 28-29 1.161213941503405 1101 30-31 1.0852469924015153 1101 32-33 0.982146098114363 1101 34-35 1.0918678911909154 1101 36-37 1.0959360838310275 1101 38-39 1.074681136280212 1101 40-41 0.9892201625986559 1101 42-43 0.9582160291170894 1101 44-45 0.8660407931889864 1101 46-47 0.9603063857089111 1101 48-49 0.8543140073504674 1101 50-51 0.7543858143946522 1101 52-53 0.7118932062888277 1101 54-55 0.6363654607719411 1101 56-57 0.4812798765319428 1101 58-59 0.543036567217527 1101 60-61 0.5656935598672774 1101 62-63 0.42365885324463903 1101 64-65 0.380228315778524 1101 66-67 0.41080963290764316 1101 68-69 0.4632368448975015 1101 70-71 0.40249721385542614 1101 72-73 0.38714923297190396 1101 74-75 0.32716903826746346 1101 76-77 0.38044977949352443 1101 78-79 0.4054593607929924 1101 80-81 0.47442595494649 1101 82-83 0.4265718970664736 1101 84-85 0.39526470052246765 1101 86-87 0.3887971619697339 1101 88-89 0.46237410998181616 1101 90-91 0.40625220655378413 1101 92-93 0.4747897857917067 1101 94-95 0.4641223694782184 1101 96-97 0.5413846339840447 1101 98-99 0.5340708334929474 1101 100-101 0.6647750803223502 1102 1 0.6566658718553811 1102 2 0.7606595335373072 1102 3 0.7391003084941374 1102 4 0.6110790473337033 1102 5 0.6467982671745958 1102 6 0.6529852225267021 1102 7 0.6822215445673621 1102 8 0.6898003176335266 1102 9 0.8611548064965646 1102 10-11 0.8793527436646471 1102 12-13 0.8268807264589171 1102 14-15 0.9854897813828458 1102 16-17 0.9505149440186784 1102 18-19 0.9140855172770159 1102 20-21 1.0086605268006892 1102 22-23 1.0991234664043077 1102 24-25 0.9258035741260002 1102 26-27 1.0381515351942667 1102 28-29 1.0628100859571177 1102 30-31 1.0538223384025969 1102 32-33 0.8869711157358608 1102 34-35 0.976949895897377 1102 36-37 1.0685476080117837 1102 38-39 1.0581276834845497 1102 40-41 0.8460698484642748 1102 42-43 0.9297708994950327 1102 44-45 0.885121496864798 1102 46-47 0.9103951753850268 1102 48-49 0.7459465487494157 1102 50-51 0.6625580490223228 1102 52-53 0.5729258583193229 1102 54-55 0.6217016937123887 1102 56-57 0.6054817060224664 1102 58-59 0.5826851640654453 1102 60-61 0.5673569643217675 1102 62-63 0.54920721245675 1102 64-65 0.4562742047436785 1102 66-67 0.4754086606265773 1102 68-69 0.5011691381154861 1102 70-71 0.4093703487981628 1102 72-73 0.414194239937256 1102 74-75 0.3259342038540254 1102 76-77 0.3851826080164855 1102 78-79 0.41035273686808793 1102 80-81 0.3833077322847842 1102 82-83 0.37012737841543597 1102 84-85 0.3478884880618054 1102 86-87 0.3941352958117008 1102 88-89 0.3181270196099035 1102 90-91 0.4686269251186097 1102 92-93 0.4377150951622717 1102 94-95 0.5013109523214467 1102 96-97 0.47863673052578903 1102 98-99 0.6283613879086829 1102 100-101 0.6923195364425112 1103 1 0.5943239037485455 1103 2 0.5943420893060853 1103 3 0.624662913560563 1103 4 0.5968509899064642 1103 5 0.6014412119208359 1103 6 0.5040393563459418 1103 7 0.5737177227544876 1103 8 0.5647899435486394 1103 9 0.7357808783700719 1103 10-11 0.6940764287609156 1103 12-13 0.6243161719866777 1103 14-15 0.7990399557596888 1103 16-17 0.8379404465624063 1103 18-19 0.856319673282961 1103 20-21 0.8615903816188322 1103 22-23 0.9645648710369557 1103 24-25 0.7875952604265848 1103 26-27 0.8651043356439061 1103 28-29 0.9297810341392605 1103 30-31 0.92514754936537 1103 32-33 0.8289000719277126 1103 34-35 0.861670154087598 1103 36-37 0.9749900761034098 1103 38-39 0.9628649401891991 1103 40-41 0.8849792082403027 1103 42-43 0.9235523560257661 1103 44-45 0.8530409072848073 1103 46-47 0.8740259679282261 1103 48-49 0.7477299115758882 1103 50-51 0.7677959360102733 1103 52-53 0.7470970035168634 1103 54-55 0.534840633600794 1103 56-57 0.41053311307112494 1103 58-59 0.41346528141709626 1103 60-61 0.43492681418801027 1103 62-63 0.48514248346933897 1103 64-65 0.37129794295525187 1103 66-67 0.31279531222239143 1103 68-69 0.34963531237429635 1103 70-71 0.2646875687186139 1103 72-73 0.2704270881169002 1103 74-75 0.2550889729822252 1103 76-77 0.32022792662998967 1103 78-79 0.30073747594371625 1103 80-81 0.2206819926125334 1103 82-83 0.3397975885621207 1103 84-85 0.3395904016424467 1103 86-87 0.32228513309994256 1103 88-89 0.34491434764215967 1103 90-91 0.35152128673431093 1103 92-93 0.39385504917510517 1103 94-95 0.5094617267353598 1103 96-97 0.577188750337065 1103 98-99 0.652698962244763 1103 100-101 0.6510192094513556 1104 1 0.5028756197993225 1104 2 0.585323517584559 1104 3 0.5305179648582907 1104 4 0.46962289929544454 1104 5 0.5477267671202881 1104 6 0.44108530737780427 1104 7 0.5222961234914223 1104 8 0.46156859520247906 1104 9 0.594391723040772 1104 10-11 0.6214599197102686 1104 12-13 0.5418274833177961 1104 14-15 0.7866244386502927 1104 16-17 0.7342244598753496 1104 18-19 0.7683691172476728 1104 20-21 0.7705261336485663 1104 22-23 0.845711406360536 1104 24-25 0.7549710666365428 1104 26-27 0.7293411135938115 1104 28-29 0.8529324342439892 1104 30-31 0.8756745271248221 1104 32-33 0.7809072033107469 1104 34-35 0.7702496930066829 1104 36-37 0.8824234793834478 1104 38-39 0.8787689568638584 1104 40-41 0.8726398666413502 1104 42-43 0.7172474528783539 1104 44-45 0.7703531803051931 1104 46-47 0.7790931066054938 1104 48-49 0.819440870966389 1104 50-51 0.7792674855051445 1104 52-53 0.6263701610873795 1104 54-55 0.6320054402508077 1104 56-57 0.5153256064440299 1104 58-59 0.5055039614634751 1104 60-61 0.5780634983211641 1104 62-63 0.5232948715873889 1104 64-65 0.5070778185281739 1104 66-67 0.4162434356086848 1104 68-69 0.435248316777205 1104 70-71 0.4311678403525079 1104 72-73 0.4558646103299715 1104 74-75 0.45440910152674974 1104 76-77 0.34932605280431517 1104 78-79 0.37225163663516625 1104 80-81 0.4473648928269789 1104 82-83 0.39538578438435223 1104 84-85 0.427625000542065 1104 86-87 0.46058379657434045 1104 88-89 0.31537243379749214 1104 90-91 0.34313376867600454 1104 92-93 0.41875017074381304 1104 94-95 0.3924235821858808 1104 96-97 0.40012143226115526 1104 98-99 0.4436933458154755 1104 100-101 0.5482980697623532 1105 1 0.3722874990404108 1105 2 0.4441457678160532 1105 3 0.3617407288651009 1105 4 0.3054222686942225 1105 5 0.4493796412617357 1105 6 0.3253221590927424 1105 7 0.2828844244089197 1105 8 0.32013469688721585 1105 9 0.4789205240461456 1105 10-11 0.5566418071208616 1105 12-13 0.38518638184671516 1105 14-15 0.45468415844825927 1105 16-17 -1.5455392101766847 1105 18-19 0.1056370677983054 1105 20-21 0.39453918798555065 1105 22-23 0.6759115683184902 1105 24-25 0.5820259889038937 1105 26-27 0.6465112715259025 1105 28-29 0.6641529762949645 1105 30-31 0.6812505333740191 1105 32-33 0.6042718484364471 1105 34-35 0.7274970800720837 1105 36-37 0.7627229999537519 1105 38-39 0.7221677270274753 1105 40-41 0.6423009421557424 1105 42-43 0.5816248814102494 1105 44-45 0.5221463356187428 1105 46-47 0.51047192720889 1105 48-49 0.5000813185542086 1105 50-51 0.40788131073607303 1105 52-53 0.40342533165021166 1105 54-55 0.47027783263435197 1105 56-57 0.4203762887484572 1105 58-59 0.3564214363607974 1105 60-61 0.22326585798281684 1105 62-63 0.3392666342704942 1105 64-65 0.3356505190983796 1105 66-67 0.3461040910302273 1105 68-69 0.3978503113636691 1105 70-71 0.30131103639806867 1105 72-73 0.3340412045428671 1105 74-75 0.30518559656002964 1105 76-77 0.2232782170054648 1105 78-79 0.19462667991364668 1105 80-81 0.31655740589829406 1105 82-83 0.33420029241630544 1105 84-85 0.22905216254319782 1105 86-87 0.16434320957148785 1105 88-89 0.26066422025229485 1105 90-91 0.3290812838618713 1105 92-93 0.25391742604399425 1105 94-95 0.39836904633559556 1105 96-97 0.3281259634804421 1105 98-99 0.29129466134267545 1105 100-101 0.24832008721971732 1106 1 0.37773716784389677 1106 2 0.47783567372669467 1106 3 0.40653708064944993 1106 4 0.3623860544354187 1106 5 0.3957242863820696 1106 6 0.36784986247412377 1106 7 0.4093035224771526 1106 8 0.4212606095242819 1106 9 0.5291677816287503 1106 10-11 0.5597486078353455 1106 12-13 0.423838315552274 1106 14-15 0.47925847320617265 1106 16-17 0.5651906414758372 1106 18-19 0.5679292261703921 1106 20-21 0.5679747783748752 1106 22-23 0.6588492577112248 1106 24-25 0.6179218099114578 1106 26-27 0.5453373811632289 1106 28-29 0.6070343894943377 1106 30-31 0.6982001007439322 1106 32-33 0.5888929109937493 1106 34-35 0.5484588753119866 1106 36-37 0.6780079434655022 1106 38-39 0.633499235263379 1106 40-41 0.6026013622391702 1106 42-43 0.576313225880071 1106 44-45 0.5473819124364709 1106 46-47 0.5809898180409263 1106 48-49 0.4835654945637202 1106 50-51 0.476940608024087 1106 52-53 0.334982676253631 1106 54-55 0.2659798413017995 1106 56-57 0.38811219602050073 1106 58-59 0.36637862573904556 1106 60-61 0.4485537950219083 1106 62-63 0.41836838521891906 1106 64-65 0.41733087399570934 1106 66-67 0.3655168684086263 1106 68-69 0.3123625749297858 1106 70-71 0.2122247938297619 1106 72-73 0.2824703625594047 1106 74-75 0.2374636673288535 1106 76-77 0.3540246761963033 1106 78-79 0.32526543220296844 1106 80-81 0.3812356211481074 1106 82-83 0.40086353084033277 1106 84-85 0.45552779127229925 1106 86-87 0.43101577847032857 1106 88-89 0.4742887987021476 1106 90-91 0.42295681674154295 1106 92-93 0.4686059967071472 1106 94-95 0.4689226333023342 1106 96-97 0.4469062654343965 1106 98-99 0.4081390664860294 1106 100-101 0.44282258206145286 1107 1 0.33739649588275 1107 2 0.39764397904420434 1107 3 0.34984618986779026 1107 4 0.3293564815090164 1107 5 0.3831648711102673 1107 6 0.23802480876879883 1107 7 0.2870017511605951 1107 8 0.3132079154491194 1107 9 0.35913358907081516 1107 10-11 0.3524552421741518 1107 12-13 0.1830027366571514 1107 14-15 0.2565765506350104 1107 16-17 0.34427733704106345 1107 18-19 0.31657862194698083 1107 20-21 0.39381238834932475 1107 22-23 0.4514038182375657 1107 24-25 0.36486484494295013 1107 26-27 0.42272362710787803 1107 28-29 0.49282659332007483 1107 30-31 0.5381589152712394 1107 32-33 0.4858164217685115 1107 34-35 0.42724512602425335 1107 36-37 0.5676945013989325 1107 38-39 0.4498932641684732 1107 40-41 0.5141684588266955 1107 42-43 0.5073017546683403 1107 44-45 0.5460560236108734 1107 46-47 0.5767769771423801 1107 48-49 0.5936334464794797 1107 50-51 0.5582493963355262 1107 52-53 0.4071268619517525 1107 54-55 0.5199208128244521 1107 56-57 0.4840597299199345 1107 58-59 0.41383457939098633 1107 60-61 0.399332581139511 1107 62-63 0.27987237132553133 1107 64-65 0.297109582548714 1107 66-67 0.3476111828506916 1107 68-69 0.37302117947604074 1107 70-71 0.3216953107500302 1107 72-73 0.28115466845725123 1107 74-75 0.33174017412410706 1107 76-77 0.3602108464882363 1107 78-79 0.35479646040344903 1107 80-81 0.29722426307662175 1107 82-83 0.27582588296692734 1107 84-85 0.3019474588480797 1107 86-87 0.3473528863319544 1107 88-89 0.3800998697240985 1107 90-91 0.34592249144044374 1107 92-93 0.4297601676680003 1107 94-95 0.49728417244057255 1107 96-97 0.44466306325784544 1107 98-99 0.5229608754256567 1107 100-101 0.5323322842315292 1108 1 0.11099817439750836 1108 2 0.13186366783192227 1108 3 0.1094516757681454 1108 4 0.0015681769817987856 1108 5 0.1659599222241539 1108 6 -0.03269802606269678 1108 7 2.68118831129982E-4 1108 8 0.10116164236026037 1108 9 0.0448734762499754 1108 10-11 0.03296274849935088 1108 12-13 -0.06096880005906513 1108 14-15 -0.02149884911396782 1108 16-17 0.06491057291068358 1108 18-19 0.17446100014375077 1108 20-21 0.06509366151894369 1108 22-23 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-0.7978522040297165 2215 40-41 -0.8473811905062263 2215 42-43 -1.027612270002649 2215 44-45 -0.8651914695755636 2215 46-47 -0.834691828960068 2215 48-49 -0.7081421365703164 2215 50-51 -0.7936127415139822 2215 52-53 -1.0724848727208283 2215 54-55 -0.8160876050475849 2215 56-57 -0.9694199958382868 2215 58-59 -0.9175277637258432 2215 60-61 -1.0741892655712846 2215 62-63 -0.9450605322849626 2215 64-65 -0.9574086262167114 2215 66-67 -1.1209796757453248 2215 68-69 -1.0687927284527987 2215 70-71 -0.9788295882832188 2215 72-73 -0.9133290031888706 2215 74-75 -0.8686730541695624 2215 76-77 -0.8199411926583764 2215 78-79 -0.84299331194687 2215 80-81 -0.7827064465264115 2215 82-83 -0.697910126806093 2215 84-85 -0.9375318222889959 2215 86-87 -0.9251190209780056 2215 88-89 -0.9493352552462042 2215 90-91 -0.8162299155079502 2215 92-93 -0.885776265077066 2215 94-95 -1.0510034458915882 2215 96-97 -1.0380554907291177 2215 98-99 -1.101096570157722 2215 100-101 -0.9501773859457145 2216 1 -1.4585706417180404 2216 2 -4.349870306069782 2216 3 -1.8903533416140235 2216 4 -1.223245296110811 2216 5 -1.216571797107484 2216 6 -1.1077653437496195 2216 7 -0.9967757862540054 2216 8 -0.8988308487873411 2216 9 -3.681676810905863 2216 10-11 -1.684749289321502 2216 12-13 -1.1703542549498778 2216 14-15 -1.4641025841161763 2216 16-17 -1.3321280017143948 2216 18-19 -1.2114019679207715 2216 20-21 -1.255628031613405 2216 22-23 -4.29558447108797 2216 24-25 -1.8909891078759102 2216 26-27 -1.3950621562007512 2216 28-29 -1.481154123913747 2216 30-31 -1.6116410043676197 2216 32-33 -1.432802404010836 2216 34-35 -1.7334000466145838 2216 36-37 -1.531471544045992 2216 38-39 -1.4748857956126287 2216 40-41 -1.188875606411436 2216 42-43 -1.5694743708071002 2216 44-45 -1.339245338017058 2216 46-47 -1.1914511100717746 2216 48-49 -1.260691901826803 2216 50-51 -1.5640276043337167 2216 52-53 -1.4806443752401819 2216 54-55 -1.3288630529896963 2216 56-57 -1.3744450413399036 2216 58-59 -1.5120934623655415 2216 60-61 -1.2453810023861607 2216 62-63 -1.178966890583233 2216 64-65 -1.1916477969873398 2216 66-67 -1.2922041819594376 2216 68-69 -1.229850208649566 2216 70-71 -1.0760743661261678 2216 72-73 -1.1456139160350638 2216 74-75 -1.0416726158718959 2216 76-77 -1.2176661729438827 2216 78-79 -1.0894676528396374 2216 80-81 -1.2778853947076954 2216 82-83 -1.08019565720803 2216 84-85 -1.1224743000316728 2216 86-87 -1.2184020841613012 2216 88-89 -1.2042940331223058 2216 90-91 -1.2632420751248539 2216 92-93 -1.2895159044667999 2216 94-95 -1.458042668915219 2216 96-97 -1.60785353219838 2216 98-99 -1.5756478213312164 2216 100-101 -1.7120254153809817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 44.0 11 443.0 12 1534.0 13 3223.0 14 4748.0 15 6126.0 16 7514.0 17 10273.0 18 16300.0 19 34400.0 20 32522.0 21 27581.0 22 29044.0 23 31913.0 24 34039.0 25 36906.0 26 42137.0 27 49946.0 28 57777.0 29 65007.0 30 72720.0 31 84302.0 32 100268.0 33 122242.0 34 152751.0 35 204070.0 36 314186.0 37 446761.0 38 381051.0 39 79216.0 40 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.678655642802635 41.498609087134064 12.315611664804086 18.507123605259217 2 21.6086571821367 25.829862345969616 37.091336407373944 15.470144064519738 3 20.173156708699604 31.421033999683967 21.552922672757585 26.852886618858847 4 25.700754273693597 23.997401506097518 29.02081612470811 21.281028095500776 5 21.31925474968458 28.752771414922158 22.475715668140673 27.45225816725259 6 20.98202163968885 23.241984540512863 29.554642480475568 26.22135133932272 7 26.353766734133327 23.917127480134628 21.822530706039863 27.906575079692182 8 32.86633394729761 23.009777833671073 23.108711691711807 21.015176527319504 9 20.46281283290141 29.663130825838437 23.567041265177448 26.307015076082706 10-11 29.823495137623407 23.49166697630283 22.640398903928812 24.04443898214495 12-13 26.841515145699805 23.599154958676845 22.75744137362496 26.80188852199839 14-15 26.48087877734261 23.486893697349544 23.048999517556908 26.983228007750938 16-17 26.59989947356322 26.12233765205032 22.87321460434354 24.404548270042916 18-19 23.605881320153035 23.609433630992484 26.112138695281146 26.67254635357334 20-21 23.38475062992245 29.60235367037264 23.803147346118653 23.20974835358626 22-23 19.95714538613563 26.23605688547744 30.031818346095317 23.77497938229162 24-25 19.81282187699945 24.757126157205615 31.84837273606627 23.581679229728664 26-27 19.80500251418016 24.424846837124612 35.76466702067303 20.005483628022198 28-29 19.876484277348496 23.284539014969464 36.944402816099675 19.894573891582368 30-31 19.869455130024257 23.27725564638922 36.847454291329306 20.005834932257212 32-33 19.900563837705874 23.226201855450288 36.83819299181866 20.03504131502518 34-35 20.08340267904464 23.489413584355283 36.541834858938984 19.885348877661098 36-37 20.11396625731356 23.577387176733666 36.108203009900066 20.200443556052704 38-39 20.44280738115741 23.709352942161228 35.457602606017474 20.39023707066389 40-41 21.345538265733712 24.49774189287379 32.775678223256264 21.38104161813623 42-43 21.456202457219153 24.946082454442635 32.37031003099085 21.22740505734736 44-45 21.95167960945829 25.522955064102852 31.000534479654046 21.524830846784813 46-47 21.855277282246497 26.237357147144415 30.531037588741384 21.376327981867707 48-49 22.141085192180082 26.73825736307488 29.461650758001124 21.65900668674391 50-51 22.13757149104379 26.82095706842112 29.143459000777028 21.898012439758062 52-53 22.03287233612523 27.130150491341553 28.943604434425392 21.89337273810783 54-55 22.1092061568463 26.818302641244106 28.865433212296214 22.207057989613375 56-57 22.221430551738976 26.688827088686466 28.833891154790454 22.2558512047841 58-59 22.41098913034158 26.742091313950244 28.66652430208349 22.18039525362468 60-61 22.27167327246499 26.83030699711038 28.58933302737197 22.308686703052658 62-63 22.307053457356567 26.722880261449973 28.707396275628177 22.262670005565287 64-65 22.450166611476572 26.960721665943275 28.391159077722488 22.197952644857665 66-67 22.46992888439928 26.82824502441907 28.246657664760804 22.45516842642085 68-69 22.585826110638603 26.886369696081307 28.00012167690372 22.527682516376363 70-71 22.782642251398855 27.05717211252911 27.668962616545745 22.491223019526295 72-73 22.696247580356857 27.192532033410828 27.449701741530557 22.661518644701754 74-75 22.720836254461314 27.185803338435864 27.54558694971359 22.547773457389233 76-77 22.72207788389809 27.42362828573843 27.388656355153124 22.46563747521035 78-79 22.80728760478382 27.29491362097759 27.296199812991762 22.60159896124683 80-81 22.77834691853493 27.27416193059441 27.28777911658557 22.659712034285093 82-83 22.877791165855694 27.43513870951541 27.095341942443607 22.591728182185292 84-85 22.988712015004644 27.25853101240717 27.21643406161875 22.536322910969442 86-87 23.022129254247766 27.167858051350063 27.221469341324262 22.588543353077913 88-89 23.081681475441496 27.2811440559452 27.07468138438805 22.562493084225256 90-91 23.126514069799203 27.265281157121912 26.96582555874356 22.642379214335325 92-93 23.012928756177587 27.197956943344543 27.137240154499114 22.65187414597876 94-95 23.212750831869812 27.45717539519175 26.809278558889932 22.5207952140485 96-97 23.18741574355047 27.54797730417336 26.66204300414476 22.602563948131408 98-99 23.32077598299551 27.45780015640537 26.735721884296776 22.48570197630234 100-101 23.464223485482805 27.656924820020766 26.23573624229988 22.643115452196554 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 573.0 1 531.5 2 436.5 3 350.0 4 270.0 5 216.0 6 185.5 7 157.5 8 157.0 9 158.5 10 164.5 11 173.0 12 180.5 13 218.5 14 259.5 15 324.5 16 1445.0 17 1780.5 18 1190.0 19 1559.0 20 1990.5 21 2573.5 22 3363.0 23 4424.0 24 5471.5 25 6888.5 26 8954.5 27 11920.0 28 15637.5 29 20462.0 30 27372.0 31 34408.0 32 41757.5 33 51539.0 34 60898.0 35 69442.5 36 76485.0 37 80739.5 38 84138.5 39 88636.0 40 89875.0 41 86706.0 42 82956.0 43 82199.5 44 84458.0 45 86750.0 46 87856.5 47 88228.5 48 87099.5 49 83251.5 50 80252.0 51 79256.5 52 81890.0 53 82473.0 54 75835.5 55 70106.0 56 65591.0 57 56748.5 58 49952.5 59 47057.5 60 42288.0 61 35918.0 62 28882.5 63 21927.0 64 16217.0 65 11339.0 66 7937.5 67 5687.0 68 4324.0 69 3495.0 70 2950.5 71 2607.0 72 2435.0 73 1973.0 74 1416.5 75 1233.5 76 1039.0 77 719.0 78 480.0 79 289.0 80 126.0 81 62.5 82 36.0 83 16.5 84 4.5 85 1.5 86 1.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 2.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 4.083114240228393E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.004797659232268362 16-17 1.6332456960913572E-4 18-19 4.083114240228393E-5 20-21 0.0 22-23 0.01496461369043706 24-25 0.0 26-27 1.0207785600570983E-4 28-29 0.007839579341238514 30-31 0.0027765176833553073 32-33 8.166228480456786E-5 34-35 0.001184103129666234 36-37 0.020068506490722553 38-39 4.083114240228393E-4 40-41 0.0018986481217062028 42-43 4.083114240228393E-5 44-45 0.0 46-47 0.0 48-49 0.009636149606939008 50-51 0.00114327198726395 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.166228480456786E-5 70-71 0.009534071750933298 72-73 0.005900100077130028 74-75 0.0 76-77 1.6332456960913572E-4 78-79 8.574539904479626E-4 80-81 0.0 82-83 0.0 84-85 1.0207785600570983E-4 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0014495055552810796 94-95 0.004491425664251232 96-97 0.00467516580506151 98-99 0.014127575271190239 100-101 0.031562473076965476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2449111.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.00490758725454 #Duplication Level Percentage of deduplicated Percentage of total 1 84.95010515984505 44.178223683652966 2 7.471090389749705 7.770667305899178 3 2.4083299400169693 3.7573492791060232 4 1.2322200406746018 2.563259573697828 5 0.7257326475843454 1.8870829635338724 6 0.5040503145432914 1.5727854016290275 7 0.33498245528400283 1.2194512141277336 8 0.25008381569417165 1.0404468579394717 9 0.19736949582206098 0.9237764151692373 >10 1.6029067299810213 17.97372873621816 >50 0.24580962227990152 8.69399037802849 >100 0.07565580475181143 5.947353554079721 >500 7.129644741836185E-4 0.2600791272289629 >1k 8.71401024002152E-4 1.0873639290481485 >5k 0.0 0.0 >10k+ 7.921827490928654E-5 1.124441580641061 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 27294 1.1144452007279375 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG 4029 0.16450867273880196 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 2894 0.1181653261122097 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 2559 0.10448689340744459 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 2555 0.10432356883783546 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.166228480456786E-5 0.0 0.0 0.0 0.0 2 1.2249342720685178E-4 0.0 0.0 0.0 0.0 3 1.2249342720685178E-4 0.0 0.0 0.0 0.0 4 1.6332456960913572E-4 0.0 0.0 0.0 0.0 5 1.6332456960913572E-4 0.0 0.0 0.0 0.0 6 1.6332456960913572E-4 0.0 0.0 0.0 0.0 7 1.6332456960913572E-4 0.0 0.0 0.0 0.0 8 1.6332456960913572E-4 0.0 0.0 0.0 0.0 9 2.4498685441370357E-4 0.0 0.0 0.0 0.0 10-11 3.674802816205554E-4 0.0 0.0 0.0 0.0 12-13 7.145449920399687E-4 0.0 0.0 0.0 0.0 14-15 0.0010616097024593821 4.083114240228393E-5 0.0 0.0 0.0 16-17 0.0013474276992753698 4.083114240228393E-5 0.0 0.0 0.0 18-19 0.0015515834112867894 4.083114240228393E-5 0.0 0.0 0.0 20-21 0.0020415571201141966 4.083114240228393E-5 0.0 0.0 0.0 22-23 0.0024090374017347515 4.083114240228393E-5 0.0 0.0 0.0 24-25 0.0028377643969587332 4.083114240228393E-5 0.0 0.0 0.0 26-27 0.003470647104194134 4.083114240228393E-5 0.0 0.0 0.0 28-29 0.0047772436610672205 4.083114240228393E-5 0.0 0.0 4.083114240228393E-5 30-31 0.005879684505928886 4.083114240228393E-5 0.0 0.0 4.083114240228393E-5 32-33 0.007043372064393978 4.083114240228393E-5 0.0 0.0 6.124671360342589E-5 34-35 0.00841121533487049 4.083114240228393E-5 0.0 0.0 8.166228480456786E-5 36-37 0.010309863456576692 4.083114240228393E-5 0.0 0.0 8.166228480456786E-5 38-39 0.012984303283926291 4.083114240228393E-5 0.0 0.0 8.166228480456786E-5 40-41 0.016475365959321565 4.083114240228393E-5 0.0 0.0 8.166228480456786E-5 42-43 0.02149759647480249 4.083114240228393E-5 0.0 0.0 8.166228480456786E-5 44-45 0.027540605550340512 4.083114240228393E-5 0.0 0.0 8.166228480456786E-5 46-47 0.03472688661314248 4.083114240228393E-5 0.0 0.0 1.2249342720685178E-4 48-49 0.04426095836407578 4.083114240228393E-5 0.0 0.0 1.2249342720685178E-4 50-51 0.05646946994235867 4.083114240228393E-5 0.0 0.0 1.2249342720685178E-4 52-53 0.0715157459176003 4.083114240228393E-5 0.0 0.0 1.2249342720685178E-4 54-55 0.08880773472496756 4.083114240228393E-5 0.0 0.0 1.2249342720685178E-4 56-57 0.10852917650527069 4.083114240228393E-5 0.0 0.0 1.6332456960913572E-4 58-59 0.13347700451306616 4.083114240228393E-5 0.0 0.0 2.0415571201141966E-4 60-61 0.16036431178497013 4.083114240228393E-5 0.0 0.0 2.0415571201141966E-4 62-63 0.19153888900911392 4.083114240228393E-5 0.0 0.0 2.0415571201141966E-4 64-65 0.2297568382976517 1.4290899840799375E-4 0.0 0.0 2.6540242561484554E-4 66-67 0.2734257450968943 1.6332456960913572E-4 0.0 0.0 3.2664913921827144E-4 68-69 0.32003449414910146 1.6332456960913572E-4 0.0 0.0 3.2664913921827144E-4 70-71 0.37529944539059273 1.6332456960913572E-4 0.0 4.083114240228393E-5 3.2664913921827144E-4 72-73 0.43746485969807003 1.6332456960913572E-4 0.0 4.083114240228393E-5 3.2664913921827144E-4 74-75 0.5051424782298557 1.6332456960913572E-4 0.0 8.166228480456786E-5 3.2664913921827144E-4 76-77 0.5839261674950624 1.6332456960913572E-4 0.0 8.166228480456786E-5 3.2664913921827144E-4 78-79 0.6728155645048346 1.6332456960913572E-4 0.0 8.166228480456786E-5 3.2664913921827144E-4 80-81 0.771810669259172 1.6332456960913572E-4 0.0 8.166228480456786E-5 3.2664913921827144E-4 82-83 0.8809931440428792 1.6332456960913572E-4 0.0 8.166228480456786E-5 3.2664913921827144E-4 84-85 1.004344025240179 1.6332456960913572E-4 0.0 8.166228480456786E-5 3.2664913921827144E-4 86-87 1.1381272633212622 1.6332456960913572E-4 0.0 8.166228480456786E-5 3.2664913921827144E-4 88-89 1.28736508880161 2.0415571201141966E-4 0.0 8.166228480456786E-5 3.2664913921827144E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 18105 0.0 92.3928 1 TATCTCG 18620 0.0 89.60779 2 GTCGACG 14005 0.0 87.49513 1 ATCTCGA 19350 0.0 86.37451 3 TCTCGAG 15650 0.0 85.06601 4 CTCGAGG 15890 0.0 83.54207 5 CTCGACG 3980 0.0 83.175804 5 CGAGGGC 16035 0.0 82.69776 7 TCGAGGG 16145 0.0 82.16374 6 AGGGCGC 16410 0.0 80.89974 9 GAGGGCG 16500 0.0 80.256966 8 TCTCGAC 4230 0.0 78.1477 4 TCGACGG 17395 0.0 70.41647 2 CGACGGC 17805 0.0 68.768295 3 CGGCGCG 18915 0.0 64.58206 6 GACGGCG 19005 0.0 64.27623 4 ACGGCGC 19115 0.0 63.831802 5 GGGCGCG 16380 0.0 40.523956 10-11 GATCCTT 13180 0.0 39.27042 20-21 GGATCCT 13865 0.0 38.20438 18-19 >>END_MODULE