FastQCFastQC Report
Tue 31 May 2016
SRR2048242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449111
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT181480.7410035723166487No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT131890.5385219371437228No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT55100.22497959463658443No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.12200345349802437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC39000.073.017491
GTCGACG35950.064.004591
TCGACGG36900.063.7166942
CGACGGC40400.058.3142553
TATCTCG49150.057.9831922
GACGGCG55000.042.9208264
GGTCGAC2550.042.8797571
CGGCGCG55300.042.6879846
ACGGCGC55500.042.5341535
ATCTCGA70600.040.433763
TCTCGAG71150.038.5857854
CTCGAGG79650.034.3487635
TCGAGGG82450.033.2974936
CGAGGGC82750.033.234187
AGGGCGC87700.031.3042019
GAGGGCG88000.031.1435078
GGGCGCG87950.024.8965668
TCCATAT62550.021.86953518-19
CGCGCCG140800.021.6911569
GCGCGCC147350.020.855888
Produced by FastQC (version 0.11.5)