FastQCFastQC Report
Tue 31 May 2016
SRR2048241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983330
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT198120.9989260486152076No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG37560.1893784695436463No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT22950.11571448019240368No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA21960.11072287516449607No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA21040.1060842119062385No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG20890.10532790811413129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC120850.092.1586761
TATCTCG127750.086.9579542
GTCGACG131750.086.372641
ATCTCGA131000.084.728093
TCTCGAG107050.083.63054
CTCGAGG109450.081.883455
CGAGGGC110300.081.037157
TCGAGGG111250.080.601286
AGGGCGC111500.080.002689
GAGGGCG112500.079.160898
CTCGACG27350.077.622965
TCGACGG150950.075.480912
CGACGGC153500.074.319823
TCTCGAC29500.072.4486854
CGGCGCG162850.069.702786
GACGGCG168050.067.574234
ACGGCGC169100.067.0984655
CGCGCCG274100.041.9016469
GGTCGAC2050.041.702171
GCGCGCC278400.041.303568
Produced by FastQC (version 0.11.5)