FastQCFastQC Report
Tue 31 May 2016
SRR2048241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983330
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT130390.6574296763523972No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT90250.4550427815845069No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT39290.1981011732792828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC25850.082.216861
GGTCGAC2100.065.658751
TCGACGG31200.061.1978652
TATCTCG35250.060.3647882
CGACGGC32100.059.6299973
GTCGACG33350.057.026681
CGGCGCG44600.042.7045676
ATCTCGA51550.041.1854363
GACGGCG47650.040.170474
TCTCGAG52200.039.944674
ACGGCGC48700.039.304375
CTCGAGG56600.036.8394285
CGAGGGC58700.035.5214927
TCGAGGG59300.035.1620866
GAGGGCG62400.033.4913678
AGGGCGC63100.032.9692849
GGGCGCG63250.025.0062478
TCCATAT51500.022.27279518-19
CGCGCCG105600.021.5444979
GGCGCGC111100.020.5206897
Produced by FastQC (version 0.11.5)