##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048241_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1983330 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17613458173879 33.0 31.0 34.0 31.0 34.0 2 32.38385493084862 34.0 31.0 34.0 31.0 34.0 3 32.459173208694466 34.0 31.0 34.0 31.0 34.0 4 36.06760801278658 37.0 35.0 37.0 35.0 37.0 5 35.87005642026289 37.0 35.0 37.0 35.0 37.0 6 35.78511997499155 37.0 35.0 37.0 35.0 37.0 7 35.70319059359764 37.0 35.0 37.0 33.0 37.0 8 35.64365385488043 37.0 35.0 37.0 33.0 37.0 9 37.16723036509305 39.0 37.0 39.0 33.0 39.0 10-11 37.17290012252121 39.0 37.0 39.0 33.0 39.0 12-13 37.152503869754405 39.0 37.0 39.0 33.0 39.0 14-15 38.46343750157563 40.0 38.0 41.0 33.5 41.0 16-17 38.47993122677517 40.0 38.0 41.0 34.0 41.0 18-19 38.512603802695466 40.0 38.0 41.0 34.0 41.0 20-21 38.56711036489137 40.0 38.0 41.0 34.0 41.0 22-23 38.551479834419894 40.0 38.0 41.0 34.0 41.0 24-25 38.54415553639586 40.0 38.0 41.0 34.0 41.0 26-27 38.38119551461431 40.0 38.0 41.0 34.0 41.0 28-29 38.27261323128274 40.0 38.0 41.0 34.0 41.0 30-31 38.044679402822524 40.0 37.0 41.0 33.0 41.0 32-33 37.866017758013044 40.0 37.0 41.0 33.0 41.0 34-35 37.761990692421335 40.0 36.0 41.0 33.0 41.0 36-37 37.70357681273414 40.0 36.0 41.0 33.0 41.0 38-39 37.6099111090943 40.0 36.0 41.0 33.0 41.0 40-41 37.41010926068783 39.0 36.0 41.0 32.0 41.0 42-43 37.38906031774843 39.0 36.0 41.0 32.0 41.0 44-45 37.217383390560315 39.0 36.0 41.0 31.0 41.0 46-47 37.16181094422008 39.0 35.5 41.0 31.0 41.0 48-49 37.04257082785013 39.0 35.0 41.0 31.0 41.0 50-51 36.911284304679505 39.0 35.0 40.5 31.0 41.0 52-53 36.65877060297581 39.0 35.0 40.0 31.0 41.0 54-55 36.4059147998568 38.0 35.0 40.0 30.5 41.0 56-57 36.015901539330315 38.0 35.0 40.0 30.0 41.0 58-59 35.76295497975627 37.5 34.5 40.0 29.5 41.0 60-61 35.44759923966259 37.0 34.0 40.0 29.0 41.0 62-63 35.06113304392109 36.5 34.0 39.5 29.0 41.0 64-65 34.81603969082301 36.0 34.0 39.0 29.0 41.0 66-67 34.645377471222645 36.0 34.0 39.0 29.0 41.0 68-69 34.336381489716786 35.0 34.0 39.0 29.0 40.0 70-71 34.072417600701854 35.0 34.0 37.5 29.0 40.0 72-73 33.79427528449628 35.0 33.5 37.0 29.0 39.5 74-75 33.51031900893951 35.0 33.0 37.0 28.5 39.0 76-77 33.18281980305849 35.0 33.0 36.0 28.0 39.0 78-79 32.94098838821578 35.0 33.0 36.0 27.5 38.5 80-81 32.735111655649845 35.0 33.0 35.5 27.0 37.0 82-83 32.528900132605266 35.0 33.0 35.0 27.0 37.0 84-85 32.31109800184538 35.0 33.0 35.0 27.0 36.0 86-87 32.10016084060645 35.0 33.0 35.0 26.0 36.0 88-89 31.958731275178614 35.0 33.0 35.0 26.5 36.0 90-91 31.8192348222434 35.0 33.0 35.0 26.0 36.0 92-93 31.72018020198353 35.0 33.0 35.0 26.0 35.0 94-95 31.61760448336888 35.0 33.0 35.0 26.0 35.0 96-97 31.467064986663843 35.0 33.0 35.0 25.0 35.0 98-99 31.286310649261594 35.0 33.0 35.0 25.0 35.0 100-101 30.632581819465244 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4920775806578064 1101 2 0.46220478799223486 1101 3 0.4246215657453263 1101 4 0.3461355489972888 1101 5 0.4180753257311167 1101 6 0.41754572896934405 1101 7 0.45351718397157015 1101 8 0.4543699299756483 1101 9 0.5721969047669617 1101 10-11 0.5186877638097371 1101 12-13 0.5120342241863156 1101 14-15 0.6342505122167168 1101 16-17 0.700883771508316 1101 18-19 0.7192519647669471 1101 20-21 0.7325519684553967 1101 22-23 0.7547878366937013 1101 24-25 0.7501425250639713 1101 26-27 0.892594843103474 1101 28-29 0.9478286479719031 1101 30-31 0.9342308330073124 1101 32-33 0.9303133153743133 1101 34-35 0.9737577795292722 1101 36-37 0.880904829776874 1101 38-39 0.7822064243350795 1101 40-41 0.8832972482421297 1101 42-43 0.786168000400643 1101 44-45 0.8378605475339711 1101 46-47 0.7637547544363628 1101 48-49 0.6772098545192762 1101 50-51 0.710106434065068 1101 52-53 0.7551213051045877 1101 54-55 0.7340397736536062 1101 56-57 0.8011113624097916 1101 58-59 0.6961513682592013 1101 60-61 0.7031485984052992 1101 62-63 0.6924051589851388 1101 64-65 0.6231131715800657 1101 66-67 0.644344567369707 1101 68-69 0.6824319319703349 1101 70-71 0.5437154433969553 1101 72-73 0.4655178440214698 1101 74-75 0.5074781511804574 1101 76-77 0.548271454615687 1101 78-79 0.5229994519539076 1101 80-81 0.49803216948190254 1101 82-83 0.4906910840954666 1101 84-85 0.5156864567012605 1101 86-87 0.5249038797984085 1101 88-89 0.557857014774072 1101 90-91 0.5228659354972223 1101 92-93 0.5047818657276153 1101 94-95 0.4962689842403485 1101 96-97 0.46622837032792575 1101 98-99 0.42663915805083974 1101 100-101 0.5580141162808125 1102 1 0.4625154588180109 1102 2 0.47861211932575287 1102 3 0.41495438833998577 1102 4 0.3061460864856258 1102 5 0.3872793292293508 1102 6 0.42015105671937647 1102 7 0.4316079045445207 1102 8 0.43241886747105696 1102 9 0.6016402165955981 1102 10-11 0.5677733683335902 1102 12-13 0.5092101297538463 1102 14-15 0.6491241413368698 1102 16-17 0.7318481741945959 1102 18-19 0.7302204472480369 1102 20-21 0.7287183710903804 1102 22-23 0.7637823659979901 1102 24-25 0.6958175179430128 1102 26-27 0.7948719104273891 1102 28-29 0.8027595012248128 1102 30-31 0.8519573206419864 1102 32-33 0.8907636792013065 1102 34-35 0.86796319702799 1102 36-37 0.778541555131774 1102 38-39 0.8022867599449128 1102 40-41 0.8282691898864556 1102 42-43 0.7337324036644048 1102 44-45 0.8729723877541247 1102 46-47 0.778852627898523 1102 48-49 0.7590716507058488 1102 50-51 0.7741863036067542 1102 52-53 0.819367330319487 1102 54-55 0.6728568216927684 1102 56-57 0.7294819616623514 1102 58-59 0.7833225027427844 1102 60-61 0.6881010854410761 1102 62-63 0.6288913142643011 1102 64-65 0.6851472414335618 1102 66-67 0.5652036817960706 1102 68-69 0.7281624487845448 1102 70-71 0.6133911880316916 1102 72-73 0.5736087972887915 1102 74-75 0.5338513409838228 1102 76-77 0.5985936514000514 1102 78-79 0.6094020156004873 1102 80-81 0.5500866654854235 1102 82-83 0.5527595060929968 1102 84-85 0.6041301916260196 1102 86-87 0.6794052590646018 1102 88-89 0.6530149165909762 1102 90-91 0.5671147666326135 1102 92-93 0.5540893366947444 1102 94-95 0.5654353065363189 1102 96-97 0.5963205342854039 1102 98-99 0.6010754389425088 1102 100-101 0.7391031208601362 1103 1 0.31928156716496403 1103 2 0.36848488881430086 1103 3 0.37645811922458705 1103 4 0.25844188820963865 1103 5 0.3015527338160666 1103 6 0.3902386596009393 1103 7 0.36123585846510053 1103 8 0.38821796152521415 1103 9 0.43886810667033416 1103 10-11 0.45214671978972376 1103 12-13 0.42816567751059154 1103 14-15 0.6012993748739817 1103 16-17 0.5727820894555222 1103 18-19 0.5614667322648828 1103 20-21 0.5103106220834235 1103 22-23 0.6496858147538873 1103 24-25 0.6167910934082386 1103 26-27 0.6713298939786867 1103 28-29 0.6770724168593176 1103 30-31 0.7211836560600275 1103 32-33 0.8054450354421121 1103 34-35 0.8234678355385938 1103 36-37 0.7311589863170411 1103 38-39 0.7969101817360666 1103 40-41 0.8183871418337105 1103 42-43 0.7501270625580148 1103 44-45 0.8314747722868319 1103 46-47 0.7939345808339766 1103 48-49 0.7282310542786377 1103 50-51 0.7675472954736122 1103 52-53 0.7196840993355309 1103 54-55 0.6772508098963712 1103 56-57 0.67391391448637 1103 58-59 0.49984573627097717 1103 60-61 0.47347385415812226 1103 62-63 0.43538396041925864 1103 64-65 0.5259937629749345 1103 66-67 0.39589628752446515 1103 68-69 0.536628632858438 1103 70-71 0.36494365880944457 1103 72-73 0.34922184344382146 1103 74-75 0.3777436986666487 1103 76-77 0.4317838573372086 1103 78-79 0.3972784707980779 1103 80-81 0.3638621787510061 1103 82-83 0.3673371214025636 1103 84-85 0.409990122681414 1103 86-87 0.45245662771282724 1103 88-89 0.3861161258045911 1103 90-91 0.38151292264656433 1103 92-93 0.38962794345970053 1103 94-95 0.437453912012014 1103 96-97 0.5082010482547688 1103 98-99 0.5753395101028538 1103 100-101 0.5780148937499625 1104 1 0.38203881427209296 1104 2 0.3550875597484051 1104 3 0.37705024007434673 1104 4 0.2632436076034992 1104 5 0.31156533762841576 1104 6 0.3040787876513207 1104 7 0.3297706006970884 1104 8 0.3371881573159854 1104 9 0.48083407705681225 1104 10-11 0.5229083479792962 1104 12-13 0.5721260788339251 1104 14-15 0.6776369068721237 1104 16-17 0.5612155083211974 1104 18-19 0.6881811039760564 1104 20-21 0.5763999935821573 1104 22-23 0.5914108154082172 1104 24-25 0.5951965275956397 1104 26-27 0.6753902726301106 1104 28-29 0.6866176462925466 1104 30-31 0.662206065556795 1104 32-33 0.7979755006036839 1104 34-35 0.8057593602225879 1104 36-37 0.717859126053483 1104 38-39 0.6765260560813644 1104 40-41 0.7242188523865494 1104 42-43 0.5883388354976873 1104 44-45 0.7921710938295234 1104 46-47 0.7411391211976195 1104 48-49 0.6912529457546484 1104 50-51 0.7040368108216768 1104 52-53 0.6998848459788505 1104 54-55 0.6328939646292397 1104 56-57 0.7024618213799556 1104 58-59 0.6656687213690446 1104 60-61 0.6264183807282606 1104 62-63 0.6368353618115918 1104 64-65 0.7862285820602608 1104 66-67 0.6527686878607 1104 68-69 0.6748636555490322 1104 70-71 0.5348687888330019 1104 72-73 0.5474267349330901 1104 74-75 0.4651649290883384 1104 76-77 0.4876242610534973 1104 78-79 0.38237364691664055 1104 80-81 0.404433716433104 1104 82-83 0.40417556996435877 1104 84-85 0.45384284094780014 1104 86-87 0.5000785928810245 1104 88-89 0.4994860667318761 1104 90-91 0.492988297795236 1104 92-93 0.4925077939134894 1104 94-95 0.49824253574572097 1104 96-97 0.46430074277887456 1104 98-99 0.36285263482518815 1104 100-101 0.5322997735599664 1105 1 0.2798487521887125 1105 2 0.24477575632516135 1105 3 0.19774747680227023 1105 4 0.19172307200416583 1105 5 0.2517510481721388 1105 6 0.21252518690764788 1105 7 0.22484622671329646 1105 8 0.19861747177603206 1105 9 0.291979749132679 1105 10-11 0.27900087061894396 1105 12-13 0.33627182706685943 1105 14-15 0.4468070578099699 1105 16-17 0.3318746494402376 1105 18-19 0.4393124462289819 1105 20-21 0.43946881942119376 1105 22-23 0.4545552433576532 1105 24-25 0.412493675735476 1105 26-27 0.6345081005895921 1105 28-29 0.5495787252503561 1105 30-31 0.578965068397828 1105 32-33 0.6504683553291315 1105 34-35 0.5799300080596694 1105 36-37 0.48752865219601915 1105 38-39 0.4507704277155469 1105 40-41 0.4949483448765477 1105 42-43 0.4442742969941449 1105 44-45 0.6321712867835245 1105 46-47 0.5529013923038022 1105 48-49 0.41877982356422194 1105 50-51 0.39857500017546243 1105 52-53 0.3820125070792173 1105 54-55 0.34437270434249 1105 56-57 0.4384753028882997 1105 58-59 0.35307478435947104 1105 60-61 0.30359310040707754 1105 62-63 0.4071036856970096 1105 64-65 0.3668885632976142 1105 66-67 0.2940821803998688 1105 68-69 0.3921828397190126 1105 70-71 0.3267541936214684 1105 72-73 0.1701510303009286 1105 74-75 0.25050876629116914 1105 76-77 0.31517115143751795 1105 78-79 0.3337497504683924 1105 80-81 0.27600792910394034 1105 82-83 0.2719995166830884 1105 84-85 0.32854286577568814 1105 86-87 0.4049631124716626 1105 88-89 0.3840588904753943 1105 90-91 0.3250148800265933 1105 92-93 0.23913506893510217 1105 94-95 0.20714405885290788 1105 96-97 0.26305338906618303 1105 98-99 0.2749678495385979 1105 100-101 0.26506509641299303 1106 1 0.17213353725723834 1106 2 0.18191011216242003 1106 3 0.180101006655228 1106 4 0.11489519054052266 1106 5 0.17602576612120657 1106 6 0.19275939790230723 1106 7 0.1409620816279613 1106 8 0.17186941077357432 1106 9 0.28739457510242516 1106 10-11 0.23692252573174244 1106 12-13 0.24893935288807256 1106 14-15 0.3613697096414086 1106 16-17 0.33522361328246575 1106 18-19 0.37488210987583415 1106 20-21 0.335785438277199 1106 22-23 0.413969595213203 1106 24-25 0.3835494652429645 1106 26-27 0.4434276134940305 1106 28-29 0.5364758199346085 1106 30-31 0.5155348166825817 1106 32-33 0.4806741673801085 1106 34-35 0.4984344439196704 1106 36-37 0.4711220740744366 1106 38-39 0.4556842611861285 1106 40-41 0.5648553478732907 1106 42-43 0.4740982448359077 1106 44-45 0.6101548748379102 1106 46-47 0.5359136605410129 1106 48-49 0.43337444971399464 1106 50-51 0.4728678700266755 1106 52-53 0.47449831159973144 1106 54-55 0.4130251766830213 1106 56-57 0.4671134329194402 1106 58-59 0.38902273684087874 1106 60-61 0.2672488846269161 1106 62-63 0.31485072412456816 1106 64-65 0.23418116894372787 1106 66-67 0.17675856747224117 1106 68-69 0.2768153027369564 1106 70-71 0.25091810157162797 1106 72-73 0.20538732352991218 1106 74-75 0.16831400791463125 1106 76-77 0.20888497738473433 1106 78-79 0.3172606637007007 1106 80-81 0.1857920439006122 1106 82-83 0.14970085419260926 1106 84-85 0.15725274284135793 1106 86-87 0.29403063701532517 1106 88-89 0.2694925301908313 1106 90-91 0.1310667738839193 1106 92-93 0.13955942066112215 1106 94-95 0.13607492601167692 1106 96-97 0.17271258754703211 1106 98-99 0.08882892059309455 1106 100-101 0.12173923854043878 1107 1 0.17316704514906434 1107 2 0.211522320526619 1107 3 0.16735101819113396 1107 4 0.1273161786896111 1107 5 0.1856839509623427 1107 6 0.1853451732439595 1107 7 0.221334079068626 1107 8 0.22199372602236167 1107 9 0.3154851343012268 1107 10-11 0.21362327205923748 1107 12-13 0.1818507204575397 1107 14-15 0.20083390601408269 1107 16-17 0.2869134322941491 1107 18-19 0.3985763092605552 1107 20-21 0.3359878161628558 1107 22-23 0.32844613591023375 1107 24-25 0.3429017579082796 1107 26-27 0.5027844660201382 1107 28-29 0.41761105813920096 1107 30-31 0.511334748884174 1107 32-33 0.6032553065605057 1107 34-35 0.480702943783605 1107 36-37 0.47914888242258513 1107 38-39 0.46829563714864975 1107 40-41 0.49967967696516524 1107 42-43 0.415306015710712 1107 44-45 0.45282568885844654 1107 46-47 0.45980413377462526 1107 48-49 0.35662645160188333 1107 50-51 0.3406805029847746 1107 52-53 0.41035487690911054 1107 54-55 0.20765658551287203 1107 56-57 0.3789040157713899 1107 58-59 0.23169518587126703 1107 60-61 0.31421554168942123 1107 62-63 0.45435230772244495 1107 64-65 0.42571512428325065 1107 66-67 0.4413992631580257 1107 68-69 0.4929043809801712 1107 70-71 0.4564248510739475 1107 72-73 0.4210046105980396 1107 74-75 0.33592731500544915 1107 76-77 0.39646293556263856 1107 78-79 0.34006796107665593 1107 80-81 0.2020958171036682 1107 82-83 0.25320678764335014 1107 84-85 0.41775450193028263 1107 86-87 0.43710844132063187 1107 88-89 0.32662195430039986 1107 90-91 0.32709800547870316 1107 92-93 0.2745739922322237 1107 94-95 0.3397919755673726 1107 96-97 0.39862480495828834 1107 98-99 0.34804056607790557 1107 100-101 0.36779550395267435 1108 1 0.10323436887838255 1108 2 0.08023957010263416 1108 3 0.07116829618672682 1108 4 0.11463250930429325 1108 5 0.13440009923345997 1108 6 0.10580435086485096 1108 7 0.15825222144074047 1108 8 0.16843519924309902 1108 9 0.22268079442079625 1108 10-11 0.13389249306341355 1108 12-13 0.21852842357900215 1108 14-15 0.28157498442809725 1108 16-17 0.19125558555217737 1108 18-19 0.24893539748358506 1108 20-21 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-0.6744403909399281 2215 28-29 -0.7164468209069099 2215 30-31 -0.7112716471829685 2215 32-33 -0.6682515793002395 2215 34-35 -0.6210984916664728 2215 36-37 -0.8784468034568036 2215 38-39 -0.7853164287131733 2215 40-41 -0.783440575002686 2215 42-43 -0.6846328572201017 2215 44-45 -0.6227748975469964 2215 46-47 -0.7714444902187836 2215 48-49 -0.9586922250488641 2215 50-51 -0.7056934444511285 2215 52-53 -0.555478717595669 2215 54-55 -0.5660938709975554 2215 56-57 -0.390365127232279 2215 58-59 -0.5446916345109827 2215 60-61 -0.7214677734698327 2215 62-63 -0.7206759894483596 2215 64-65 -0.6356911609263278 2215 66-67 -0.7333749422548266 2215 68-69 -0.44259179728294384 2215 70-71 -0.4430211201082628 2215 72-73 -0.5062412264154332 2215 74-75 -0.6070757076763229 2215 76-77 -0.4314429960941908 2215 78-79 -0.3377438193528377 2215 80-81 -0.42244514709813785 2215 82-83 -0.4355719919391774 2215 84-85 -0.5613949511637557 2215 86-87 -0.3963897106215768 2215 88-89 -0.5298478020460706 2215 90-91 -0.6181649056720637 2215 92-93 -0.5710208440845079 2215 94-95 -0.5644961142376168 2215 96-97 -0.7624964364392426 2215 98-99 -0.7487690412324781 2215 100-101 -0.8366824423850083 2216 1 -0.5146449056751479 2216 2 -0.5362050643433633 2216 3 -0.637115643161696 2216 4 -0.4627842655220391 2216 5 -0.5741435516977376 2216 6 -0.5920825237508112 2216 7 -0.6724250699073835 2216 8 -1.1300736737010624 2216 9 -0.9284696778229318 2216 10-11 -0.8476299842487265 2216 12-13 -0.7585988556372669 2216 14-15 -0.9566946021002707 2216 16-17 -1.0238772037091337 2216 18-19 -0.9637104254991584 2216 20-21 -1.0410265400592067 2216 22-23 -0.8830160048837357 2216 24-25 -0.9270633478814005 2216 26-27 -0.8797921513163871 2216 28-29 -0.9516530004446153 2216 30-31 -0.9044141418519089 2216 32-33 -0.8324987967987241 2216 34-35 -0.8785138469359026 2216 36-37 -1.0545073931581825 2216 38-39 -0.9338822145167924 2216 40-41 -0.9077686807422367 2216 42-43 -0.9312313222330104 2216 44-45 -0.7727811958527511 2216 46-47 -1.0225597402165363 2216 48-49 -1.182022049326136 2216 50-51 -1.1249356682929204 2216 52-53 -1.0082243290261985 2216 54-55 -1.0550956795110622 2216 56-57 -0.934457316433388 2216 58-59 -0.9618338232622108 2216 60-61 -0.9281804177685231 2216 62-63 -1.0587955852770818 2216 64-65 -1.06059421200559 2216 66-67 -0.9670542325257401 2216 68-69 -0.832424982156212 2216 70-71 -0.7899488965364085 2216 72-73 -0.9147551861602636 2216 74-75 -0.946499502675465 2216 76-77 -0.9588586212910144 2216 78-79 -0.95927862648972 2216 80-81 -0.8582451668928144 2216 82-83 -0.768198115511936 2216 84-85 -0.7467959283009051 2216 86-87 -0.7294351214060768 2216 88-89 -0.8907110298377958 2216 90-91 -0.8822870568134071 2216 92-93 -0.8390214019344455 2216 94-95 -0.8421038377599892 2216 96-97 -0.9634150893216145 2216 98-99 -1.0719589531461722 2216 100-101 -1.4491022514563703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 6.0 13 76.0 14 372.0 15 877.0 16 1683.0 17 2872.0 18 5332.0 19 10203.0 20 10781.0 21 9925.0 22 9729.0 23 10188.0 24 11342.0 25 13520.0 26 16503.0 27 21013.0 28 26239.0 29 33140.0 30 42358.0 31 54411.0 32 71723.0 33 96440.0 34 132862.0 35 188445.0 36 302250.0 37 460147.0 38 372444.0 39 78392.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.5621271914331 39.758374630345486 14.332401417051042 20.34709676117038 2 22.855298916468765 28.87295608900183 30.30988287375273 17.961862120776672 3 20.933228459207495 28.14276998784872 24.873520795833272 26.050480757110517 4 22.87203844040074 26.779960974724325 26.360363630863244 23.987636954011688 5 23.535183528749762 27.117168174433743 25.152499437801705 24.195148859014786 6 23.823492634602044 25.93302771906614 25.961162248503527 24.282317397828297 7 24.52995719320537 27.077944668814567 24.22516676498616 24.166931372993904 8 25.502563869855244 25.810228252484457 25.361992205028915 23.325215672631384 9 23.633132156524635 26.18843056879087 25.908648585964013 24.269788688720485 10-11 25.128344753520594 25.9904050258908 24.980789883680476 23.90046033690813 12-13 24.240343261081108 26.100346387136785 25.261908003206727 24.397402348575376 14-15 23.835544261418924 26.02590088386703 25.57683290224017 24.56172195247387 16-17 24.083737955862112 26.277069373225835 25.40936203254123 24.22983063837082 18-19 23.263778594585872 26.33351484624344 26.688145694362515 23.714560864808178 20-21 22.378272904660342 27.07527239541579 26.78316266077758 23.763292039146283 22-23 21.899860335899724 26.555641269985326 28.037038717712132 23.507459676402817 24-25 21.746406296481172 25.861026657187658 29.688075106008583 22.704491940322587 26-27 21.835636835973418 25.525905296833283 30.440926685510185 22.197531181683114 28-29 21.806179506184044 25.758850014873975 30.42289987041995 22.01207060852203 30-31 21.80122291315925 25.760892090292074 30.355060285978347 22.08282471057033 32-33 21.750944119233814 25.744303771939116 30.28701728910469 22.217734819722388 34-35 21.990398236458113 25.98927585418409 30.20045313789293 21.81987277146487 36-37 21.928450148174573 26.03258958227502 29.9095322070133 22.12942806253711 38-39 22.081555283678277 25.9848149286339 29.814788717658864 22.118841070028957 40-41 22.172011977853376 26.222730795274806 29.112445905289448 22.49281132158237 42-43 22.28545483743372 26.475933524913536 28.84661877917915 22.391992858473593 44-45 22.26283573585838 26.402893114106075 28.82432575516934 22.50994539486621 46-47 22.36470985665522 26.937322583735433 28.470098269072718 22.227869290536624 48-49 22.44349150166639 27.016507590769063 28.077576600969078 22.462424306595473 50-51 22.465777253407147 26.68080954757907 28.18961040270656 22.66380279630722 52-53 22.403267435710365 27.050227168563563 28.134162309934457 22.412343085791615 54-55 22.40892841836709 26.89635612832963 28.05120680875094 22.643508644552345 56-57 22.39932336020733 27.04063368173728 27.929769629864925 22.630273328190466 58-59 22.666954062107667 27.18554148830502 27.591222842391332 22.55628160719598 60-61 22.500472059735912 27.2086660335562 27.579834818168138 22.71102708853975 62-63 22.438745217670824 26.9586592460465 27.763565427305448 22.839030108977227 64-65 22.61219227111506 27.30503570567751 27.430240051473458 22.65253197173397 66-67 22.541939341928856 27.089882875901093 27.544710003272353 22.823467778897697 68-69 22.613121701391734 27.214528183208188 27.192343132151052 22.980006983249027 70-71 22.779507055444476 27.216396291785543 27.21647192229822 22.787624730471762 72-73 22.576556240402816 27.297254440071605 27.031489220525383 23.094700099000192 74-75 22.70170506254888 27.307496638227953 27.084689428733206 22.90610887048997 76-77 22.645685759332675 27.440104430585578 27.053758659061266 22.860451151020484 78-79 22.834751869778177 27.32640439141673 26.822502733416915 23.016341005388178 80-81 22.625150882910827 27.495631076282972 26.850174908209745 23.029043132596456 82-83 22.668340619059865 27.637306953457063 26.658927157860766 23.0354252696223 84-85 22.688539650118653 27.46989342920579 26.833640098632124 23.007926822043437 86-87 22.69125662396071 27.422617517004234 26.845759404637654 23.0403664543974 88-89 22.73978611728759 27.544483268039105 26.749532352155214 22.96619826251809 90-91 22.770788235786487 27.524239852642268 26.709748532801264 22.99522337876998 92-93 22.69521068300577 27.462039898663814 26.74364108900345 23.099108329326963 94-95 22.828838167997432 27.777746965183564 26.47127336238905 22.922141504429955 96-97 22.89286200481009 27.661861616574146 26.426388951914205 23.018887426701557 98-99 22.863784656737053 27.48533839486338 26.639058168183606 23.011818780215965 100-101 22.831542230661487 27.809503449263406 26.233914613965858 23.125039706109245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 574.0 1 461.5 2 272.0 3 164.5 4 124.5 5 105.0 6 97.5 7 96.0 8 95.5 9 97.0 10 106.0 11 114.5 12 121.5 13 184.0 14 253.5 15 338.0 16 1293.0 17 1796.0 18 1508.5 19 1691.5 20 1958.0 21 2473.5 22 3038.5 23 3686.0 24 4552.5 25 5751.0 26 7150.5 27 9257.0 28 11913.5 29 15075.5 30 19641.0 31 24394.5 32 28849.0 33 35328.0 34 42019.5 35 48048.5 36 53127.0 37 57046.5 38 61824.5 39 67975.0 40 69881.5 41 66578.0 42 63795.5 43 64181.5 44 67975.5 45 73159.5 46 76821.0 47 78346.5 48 77047.5 49 73801.0 50 72152.0 51 72738.0 52 76633.0 53 77343.0 54 68794.5 55 61838.5 56 57766.5 57 47807.5 58 40316.5 59 37976.0 60 34346.0 61 28821.5 62 22213.5 63 16618.5 64 12036.5 65 7763.5 66 5080.0 67 3588.5 68 2696.0 69 2123.5 70 1838.0 71 1735.5 72 1773.5 73 1505.0 74 1090.0 75 934.0 76 758.5 77 510.5 78 339.5 79 180.0 80 55.5 81 23.0 82 19.5 83 7.5 84 1.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08823544241250825 2 0.0 3 0.0 4 0.0 5 0.0022184911235144933 6 1.0084050561429515E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0077899290587043 28-29 0.0 30-31 0.00166386834263587 32-33 0.0 34-35 0.002445382261146657 36-37 0.0013865569521965583 38-39 2.5210126403573786E-5 40-41 1.512607584214427E-4 42-43 2.5210126403573786E-5 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.042025280714757E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 2.5210126403573786E-5 62-63 0.00592437970483984 64-65 0.008672283482829383 66-67 0.0024705923875502312 68-69 6.302531600893447E-4 70-71 1.7647088482501653E-4 72-73 2.5210126403573786E-5 74-75 5.042025280714757E-5 76-77 1.512607584214427E-4 78-79 3.2773164324645924E-4 80-81 2.016810112285903E-4 82-83 0.0 84-85 2.5210126403573786E-5 86-87 0.0 88-89 0.0 90-91 0.006504212612122037 92-93 0.001537817710618001 94-95 7.058835393000661E-4 96-97 0.0 98-99 1.7647088482501653E-4 100-101 4.033620224571806E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1983330.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.94963217269793 #Duplication Level Percentage of deduplicated Percentage of total 1 85.05134499578091 38.23026673353584 2 7.1425330398665094 6.421084658466832 3 2.215028636849963 2.9869416743519506 4 1.1150206687741264 2.004790757054105 5 0.679257044271546 1.526617714535999 6 0.4458792470702929 1.20252648895495 7 0.34122847663126576 1.0736666157997812 8 0.26605481171399004 0.9567252739456205 9 0.21850417048777734 0.8839513882463462 >10 2.017193435375906 19.077727649165563 >50 0.31986825190291623 10.143403586212644 >100 0.18661343164332342 13.707010583982054 >500 9.069474655209561E-4 0.29795548217446394 >1k 3.4010529957035853E-4 0.36297877535979245 >5k 1.1336843319011951E-4 0.4599022108131584 >10k+ 1.1336843319011951E-4 0.6644504074008611 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 13039 0.6574296763523972 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9025 0.4550427815845069 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 3929 0.1981011732792828 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.0084050561429515E-4 0.0 0.0 5.042025280714757E-5 0.0 8 1.512607584214427E-4 0.0 0.0 5.042025280714757E-5 0.0 9 3.025215168428854E-4 0.0 0.0 5.042025280714757E-5 0.0 10-11 9.57984803335804E-4 0.0 0.0 5.042025280714757E-5 0.0 12-13 0.002243701249918067 0.0 0.0 5.042025280714757E-5 0.0 14-15 0.0026722733987788218 0.0 0.0 5.042025280714757E-5 0.0 16-17 0.0028991645364109856 0.0 0.0 5.042025280714757E-5 0.0 18-19 0.0034285771908860348 0.0 0.0 5.042025280714757E-5 0.0 20-21 0.004033620224571806 0.0 0.0 5.042025280714757E-5 0.0 22-23 0.0053445467975576436 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 24-25 0.006554632864929185 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 26-27 0.007462197415457841 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 28-29 0.008697493609232956 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 30-31 0.01018489106704381 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 32-33 0.011596658145643942 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 34-35 0.013739518889947715 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 36-37 0.016815154311183717 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 38-39 0.021075665673387686 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 40-41 0.02687399474620966 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 42-43 0.034663923804913954 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 44-45 0.0426051136220397 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 46-47 0.05238664266662633 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 48-49 0.06536985776446683 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 50-51 0.0830925766261792 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 52-53 0.10351277901307397 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 54-55 0.12640357378751896 0.0 0.0 5.042025280714757E-5 5.042025280714757E-5 56-57 0.15110949766302129 0.0 0.0 7.563037921072136E-5 5.042025280714757E-5 58-59 0.18098349745125622 0.0 0.0 1.0084050561429515E-4 5.042025280714757E-5 60-61 0.2163028845426631 0.0 0.0 1.0084050561429515E-4 5.042025280714757E-5 62-63 0.2542945450328488 0.0 0.0 1.0084050561429515E-4 5.042025280714757E-5 64-65 0.2988156282615601 0.0 0.0 1.0084050561429515E-4 5.042025280714757E-5 66-67 0.34878209879344335 0.0 0.0 1.0084050561429515E-4 5.042025280714757E-5 68-69 0.40739564268175243 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 70-71 0.46890835110647244 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 72-73 0.5367235911320859 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 74-75 0.6131102741349146 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 76-77 0.6974129368284652 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 78-79 0.7926063741283599 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 80-81 0.8925141050657228 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 82-83 1.005657152364962 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 84-85 1.1305985388210735 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 86-87 1.2680693580997615 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 88-89 1.411943549484957 5.042025280714757E-5 0.0 1.0084050561429515E-4 5.042025280714757E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 2585 0.0 82.21686 1 GGTCGAC 210 0.0 65.65875 1 TCGACGG 3120 0.0 61.197865 2 TATCTCG 3525 0.0 60.364788 2 CGACGGC 3210 0.0 59.629997 3 GTCGACG 3335 0.0 57.02668 1 CGGCGCG 4460 0.0 42.704567 6 ATCTCGA 5155 0.0 41.185436 3 GACGGCG 4765 0.0 40.17047 4 TCTCGAG 5220 0.0 39.94467 4 ACGGCGC 4870 0.0 39.30437 5 CTCGAGG 5660 0.0 36.839428 5 CGAGGGC 5870 0.0 35.521492 7 TCGAGGG 5930 0.0 35.162086 6 GAGGGCG 6240 0.0 33.491367 8 AGGGCGC 6310 0.0 32.969284 9 GGGCGCG 6325 0.0 25.006247 8 TCCATAT 5150 0.0 22.272795 18-19 CGCGCCG 10560 0.0 21.544497 9 GGCGCGC 11110 0.0 20.520689 7 >>END_MODULE