FastQCFastQC Report
Tue 31 May 2016
SRR2048240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5718241
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT111440.19488510540216825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG163150.091.127791
TATCTCG72150.084.0530242
TCGACGG180850.082.4922
CGACGGC190650.078.17553
ACGGCGC191250.078.063635
CGGCGCG191600.077.802776
GACGGCG192650.077.481844
ATCTCGA82300.073.570043
ATATCTC86000.070.8760761
TCTCGAG70500.067.340664
TCGAGGG70650.067.105186
AGGGCGC72200.065.716559
CTCGAGG73250.064.998615
CGAGGGC76100.062.4214747
GAGGGCG79350.059.680018
CGCGCCG253000.059.3216259
GGCGCGC254050.059.104967
GCGCGCC254600.058.990788
CTCGACG23300.056.0816465
TCTCGAC23650.055.660824
Produced by FastQC (version 0.11.5)