##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5718241 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.220368641335682 31.0 31.0 34.0 28.0 34.0 2 31.229739180282888 31.0 31.0 34.0 28.0 34.0 3 31.447208678333073 31.0 31.0 34.0 28.0 34.0 4 35.184490825063165 37.0 35.0 37.0 32.0 37.0 5 35.11983667704806 37.0 35.0 37.0 32.0 37.0 6 35.1494655436873 37.0 35.0 37.0 32.0 37.0 7 34.9277846106871 37.0 35.0 37.0 32.0 37.0 8 35.131336192371045 37.0 35.0 37.0 32.0 37.0 9 36.76910854229474 39.0 37.0 39.0 32.0 39.0 10-11 36.648548478456924 39.0 37.0 39.0 32.0 39.0 12-13 36.4192567259757 38.5 36.0 39.0 32.0 39.0 14-15 37.70192599437485 40.0 37.0 41.0 32.0 41.0 16-17 37.80347724064096 40.0 37.5 41.0 32.0 41.0 18-19 37.720908492664094 40.0 37.0 41.0 32.0 41.0 20-21 37.72126445877325 40.0 37.0 41.0 32.0 41.0 22-23 37.87717026966859 40.0 37.5 41.0 32.0 41.0 24-25 37.67537893208768 40.0 37.0 41.0 32.0 41.0 26-27 37.800999730511535 40.0 37.0 41.0 32.0 41.0 28-29 37.705497722114195 40.0 37.0 41.0 32.0 41.0 30-31 37.460384495861575 40.0 36.5 41.0 31.0 41.0 32-33 37.26186391934163 39.5 35.5 40.5 31.5 41.0 34-35 37.22668002275525 39.5 35.5 41.0 31.0 41.0 36-37 36.86114523679572 38.5 35.0 40.0 30.0 41.0 38-39 36.76905398006135 39.0 35.0 40.0 30.0 41.0 40-41 36.64946729247683 39.0 35.0 40.5 29.5 41.0 42-43 36.6344598802324 39.0 35.0 40.5 29.5 41.0 44-45 36.43920770390755 39.0 35.0 40.0 29.0 41.0 46-47 36.32079690240408 39.0 35.0 40.0 29.0 41.0 48-49 36.19184194929874 38.5 35.0 40.0 29.0 41.0 50-51 36.13352427783299 38.0 34.0 40.0 29.0 41.0 52-53 35.95528266472155 38.0 34.0 40.0 28.0 41.0 54-55 35.902256568060004 38.0 34.0 40.0 28.0 41.0 56-57 35.838275616575096 38.0 34.0 40.0 28.0 41.0 58-59 35.72553369121728 38.0 34.0 40.0 28.0 41.0 60-61 35.50333231845248 38.0 34.0 40.0 28.0 41.0 62-63 35.20974605652333 37.0 34.0 40.0 28.0 41.0 64-65 34.881542943013415 37.0 33.5 40.0 28.0 41.0 66-67 34.55397770048516 36.0 33.0 39.0 27.5 41.0 68-69 34.213854662648885 36.0 33.0 39.0 26.5 41.0 70-71 33.82997953391612 35.0 33.0 39.0 26.0 40.0 72-73 33.54357520083536 35.0 32.5 37.5 26.0 40.0 74-75 33.49748060636129 35.0 33.0 37.0 27.0 39.0 76-77 33.21221709613148 35.0 33.0 37.0 27.0 39.0 78-79 32.909014502886464 35.0 33.0 36.0 26.5 39.0 80-81 32.62423112282256 35.0 33.0 36.0 26.0 37.0 82-83 32.34742117374906 35.0 32.0 35.5 26.0 37.0 84-85 32.13073320974055 35.0 32.0 35.0 26.0 37.0 86-87 31.93482978419413 35.0 32.0 35.0 26.0 36.0 88-89 31.761696560183456 35.0 32.0 35.0 25.5 36.0 90-91 31.593137470071653 35.0 32.0 35.0 25.0 36.0 92-93 31.447788402062802 35.0 32.0 35.0 25.0 35.5 94-95 31.307368472227736 35.0 32.0 35.0 25.0 35.0 96-97 31.147726634816543 35.0 32.0 35.0 24.5 35.0 98-99 30.977064537853508 35.0 32.0 35.0 24.0 35.0 100-101 30.389694313338666 34.5 31.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5253388348354022 1101 2 0.5263420390506077 1101 3 0.5555338567256207 1101 4 0.4848008420798422 1101 5 0.5282934736774507 1101 6 0.5893469558420961 1101 7 0.6509108264774213 1101 8 0.6411402310774221 1101 9 0.6858304403978082 1101 10-11 0.7535049597886996 1101 12-13 0.6883403914873583 1101 14-15 0.8621621438736398 1101 16-17 0.8213020163050047 1101 18-19 0.887317984329961 1101 20-21 0.8919294047609938 1101 22-23 0.8469920372008986 1101 24-25 0.8118398941336338 1101 26-27 0.7785359660423268 1101 28-29 0.8996156256093428 1101 30-31 0.8429856396855797 1101 32-33 0.8459344419348369 1101 34-35 0.9414718556800565 1101 36-37 0.9244353384135024 1101 38-39 0.8091386112272616 1101 40-41 0.762979898709041 1101 42-43 0.8455010944207473 1101 44-45 0.8178487659140856 1101 46-47 0.7520999083107327 1101 48-49 0.7504016048030948 1101 50-51 0.6573504544611595 1101 52-53 0.6763872158705624 1101 54-55 0.5774425580205289 1101 56-57 0.5706447557188241 1101 58-59 0.5410915135913115 1101 60-61 0.5276147505908924 1101 62-63 0.42671149372559825 1101 64-65 0.4602767268914931 1101 66-67 0.4903101702559951 1101 68-69 0.45950798938900306 1101 70-71 0.48834597165136273 1101 72-73 0.46335929964346434 1101 74-75 0.4691473600259286 1101 76-77 0.4380581295594723 1101 78-79 0.5445632244873693 1101 80-81 0.47851896693810403 1101 82-83 0.5277181286670256 1101 84-85 0.47864382899150115 1101 86-87 0.4580542500150493 1101 88-89 0.4810250070699027 1101 90-91 0.500191067332814 1101 92-93 0.5050429735622011 1101 94-95 0.492776718407935 1101 96-97 0.5511358354458373 1101 98-99 0.5041416059123094 1101 100-101 0.5654916920794335 1102 1 0.3523561779751283 1102 2 0.38899010647329746 1102 3 0.46238100800486137 1102 4 0.3871959969622836 1102 5 0.36481132696737717 1102 6 0.38028081818912085 1102 7 0.39573208580522845 1102 8 0.41546328161702917 1102 9 0.517459605277196 1102 10-11 0.5549682386662553 1102 12-13 0.5725098019248662 1102 14-15 0.706895532047561 1102 16-17 0.6315716510840232 1102 18-19 0.6665578771289589 1102 20-21 0.7579463445261467 1102 22-23 0.7151533003161603 1102 24-25 0.5649605761600114 1102 26-27 0.5461532984368773 1102 28-29 0.7093472636957614 1102 30-31 0.6678779204780554 1102 32-33 0.7095949824141528 1102 34-35 0.7374303809216158 1102 36-37 0.7919057667294425 1102 38-39 0.7197075005194051 1102 40-41 0.5962013747927983 1102 42-43 0.5835389973696294 1102 44-45 0.5952140508742687 1102 46-47 0.5634106002942261 1102 48-49 0.5431157985270616 1102 50-51 0.4842048577128679 1102 52-53 0.5583456475938107 1102 54-55 0.48884971074556915 1102 56-57 0.435824989323649 1102 58-59 0.30971681114819205 1102 60-61 0.35475124557276416 1102 62-63 0.3888886624130876 1102 64-65 0.30620071141761684 1102 66-67 0.334165318170875 1102 68-69 0.3183974360273538 1102 70-71 0.3015406765803803 1102 72-73 0.36906445786707565 1102 74-75 0.2777725149694277 1102 76-77 0.28176425058733656 1102 78-79 0.2583916041161629 1102 80-81 0.23459432778376765 1102 82-83 0.2932597601567437 1102 84-85 0.24099948570421503 1102 86-87 0.2451560333913072 1102 88-89 0.27823910381267325 1102 90-91 0.23744172801595198 1102 92-93 0.29373626901610805 1102 94-95 0.30112330597499337 1102 96-97 0.3475529774597099 1102 98-99 0.3164520463962823 1102 100-101 0.3949916555449988 1103 1 0.3005298209427245 1103 2 0.3205248523651143 1103 3 0.3310640005383725 1103 4 0.3229327655091012 1103 5 0.3471570267483699 1103 6 0.37485524307787443 1103 7 0.3796012531178121 1103 8 0.47986385936641796 1103 9 0.5113779096684397 1103 10-11 0.5390311971549337 1103 12-13 0.4844530480326128 1103 14-15 0.6252508721652177 1103 16-17 0.5684620492368353 1103 18-19 0.6116942601466988 1103 20-21 0.6441640085337283 1103 22-23 0.5856045340294997 1103 24-25 0.539196212980606 1103 26-27 0.6218307045196383 1103 28-29 0.6756768951022991 1103 30-31 0.6149910500865516 1103 32-33 0.7103114111180417 1103 34-35 0.7611091390477256 1103 36-37 0.7407970209147834 1103 38-39 0.6871791581147804 1103 40-41 0.6835149724806016 1103 42-43 0.7238414841438612 1103 44-45 0.6941534461391043 1103 46-47 0.6472701287751548 1103 48-49 0.5756924963418157 1103 50-51 0.6008402166534665 1103 52-53 0.6921238963883596 1103 54-55 0.6062284879249304 1103 56-57 0.5367657959993863 1103 58-59 0.5564797433111579 1103 60-61 0.5179076534681784 1103 62-63 0.5242988609473542 1103 64-65 0.4482249316394302 1103 66-67 0.4961669811347278 1103 68-69 0.45021529857901754 1103 70-71 0.5110766483359228 1103 72-73 0.5298570944823311 1103 74-75 0.4843415997868874 1103 76-77 0.5293303851267979 1103 78-79 0.4839229435349566 1103 80-81 0.4660248727051055 1103 82-83 0.45014541132270836 1103 84-85 0.4734764075690592 1103 86-87 0.4813437441886812 1103 88-89 0.42826937280783284 1103 90-91 0.41256024445880257 1103 92-93 0.4620054435151495 1103 94-95 0.4425623834056367 1103 96-97 0.3816946517297417 1103 98-99 0.3631607992683996 1103 100-101 0.46711836222659286 1104 1 0.2082541466224015 1104 2 0.2201586436438383 1104 3 0.24755351616149213 1104 4 0.2633048625515002 1104 5 0.223288972712254 1104 6 0.2782209748106368 1104 7 0.38261060132420965 1104 8 0.4027850354006901 1104 9 0.4363340040638093 1104 10-11 0.3999983572969583 1104 12-13 0.38596512697145613 1104 14-15 0.5271981256886775 1104 16-17 0.4801241047183211 1104 18-19 0.48666531581253736 1104 20-21 0.5910666779155562 1104 22-23 0.4921765491403818 1104 24-25 0.4404184630453827 1104 26-27 0.4496099689567785 1104 28-29 0.5465025104910524 1104 30-31 0.47300746947971817 1104 32-33 0.5379419319320462 1104 34-35 0.5645476462265933 1104 36-37 0.5616774250300907 1104 38-39 0.5558624615016114 1104 40-41 0.48050090248260346 1104 42-43 0.4865215725303642 1104 44-45 0.44058798379808195 1104 46-47 0.4024173194215521 1104 48-49 0.5092314957396766 1104 50-51 0.43531109488291975 1104 52-53 0.3753404926925299 1104 54-55 0.2893767731173753 1104 56-57 0.2827322712006506 1104 58-59 0.40210514519599627 1104 60-61 0.353617325675998 1104 62-63 0.2866729803750516 1104 64-65 0.3573853785360228 1104 66-67 0.31186411138583736 1104 68-69 0.32308130166123306 1104 70-71 0.33555750022293296 1104 72-73 0.26723101983693454 1104 74-75 0.3250756542391642 1104 76-77 0.3269432365340137 1104 78-79 0.2990476692744082 1104 80-81 0.20789926139122628 1104 82-83 0.18032339424495802 1104 84-85 0.2711094438838799 1104 86-87 0.23090300878943637 1104 88-89 0.28422783991328515 1104 90-91 0.2539942427644988 1104 92-93 0.33519969148691686 1104 94-95 0.2787115429332694 1104 96-97 0.30493926330036913 1104 98-99 0.3210869766379183 1104 100-101 0.38338363522285945 1105 1 0.17542649776894592 1105 2 0.18211712301054206 1105 3 0.23806004230254985 1105 4 0.20419507528716707 1105 5 0.23508080058626746 1105 6 0.3464144354901464 1105 7 0.33457538154360833 1105 8 0.25916691195092056 1105 9 0.3548690338903384 1105 10-11 0.3973700144985912 1105 12-13 0.32901252418799487 1105 14-15 0.4131878973130725 1105 16-17 0.42943959947711363 1105 18-19 0.4538964198957629 1105 20-21 0.4819047661187241 1105 22-23 0.48106517724899334 1105 24-25 0.3511752282087528 1105 26-27 0.44484632572792293 1105 28-29 0.5267947818491479 1105 30-31 0.4656700968604994 1105 32-33 0.39996278793499584 1105 34-35 0.4949713766357249 1105 36-37 0.5380883530763185 1105 38-39 0.4230637119745637 1105 40-41 0.4704768467916338 1105 42-43 0.5255249984094519 1105 44-45 0.36809030309476043 1105 46-47 0.4349130580282363 1105 48-49 0.4735419237669447 1105 50-51 0.33474443080399396 1105 52-53 0.3354830859151008 1105 54-55 0.32132485594818405 1105 56-57 0.3160632790043678 1105 58-59 0.27836171137829524 1105 60-61 0.2503484004859189 1105 62-63 0.2499678311366509 1105 64-65 0.19961414204462358 1105 66-67 0.25148795073808117 1105 68-69 0.26658173869745383 1105 70-71 0.3029006810780359 1105 72-73 0.34279271261272015 1105 74-75 0.29512860151911013 1105 76-77 0.30332864393457015 1105 78-79 0.25852577142558886 1105 80-81 0.198439045614748 1105 82-83 0.27627913920150604 1105 84-85 0.2119604278150291 1105 86-87 0.20703948995277344 1105 88-89 0.22219355945750507 1105 90-91 0.21472412033503474 1105 92-93 0.27865216931077796 1105 94-95 0.21248662802572937 1105 96-97 0.2056639903171522 1105 98-99 0.2112542548629932 1105 100-101 0.3064557174418958 1106 1 0.09472346517803132 1106 2 0.09221502158862194 1106 3 0.19692766530494055 1106 4 0.10334469149454151 1106 5 0.08632730870253624 1106 6 0.10825412342269658 1106 7 0.13052051807311216 1106 8 0.2153677424529974 1106 9 0.23922942145797066 1106 10-11 0.27252057445832634 1106 12-13 0.2810314621520078 1106 14-15 0.3326220863152116 1106 16-17 0.30970366138726035 1106 18-19 0.2970538377892993 1106 20-21 0.3365733202901069 1106 22-23 0.2955915393982309 1106 24-25 0.20477061011388287 1106 26-27 0.2677703222045835 1106 28-29 0.39579604018562975 1106 30-31 0.3134310758462746 1106 32-33 0.3208896785855515 1106 34-35 0.3695241638510183 1106 36-37 0.42427106353309085 1106 38-39 0.40189805197707784 1106 40-41 0.3507145465525454 1106 42-43 0.3786101248722673 1106 44-45 0.2950076933049033 1106 46-47 0.27455701172146263 1106 48-49 0.31100398492210246 1106 50-51 0.24712407091976019 1106 52-53 0.32087725981720894 1106 54-55 0.2192706216459328 1106 56-57 0.19055447064901898 1106 58-59 0.22246929914726365 1106 60-61 0.23057685147371387 1106 62-63 0.2304826795071122 1106 64-65 0.14205166563843363 1106 66-67 0.16463844778098746 1106 68-69 0.2125714203383282 1106 70-71 0.18264681169335972 1106 72-73 0.12082297560504429 1106 74-75 0.05373241427864173 1106 76-77 0.1084254729266334 1106 78-79 0.14351243305890904 1106 80-81 0.12314151195424472 1106 82-83 0.09240453243165803 1106 84-85 0.10454391844042021 1106 86-87 0.024232078350916453 1106 88-89 0.040338331069435185 1106 90-91 -0.04468314026645004 1106 92-93 0.02500620227919015 1106 94-95 -0.020941206377191435 1106 96-97 0.08145328187371703 1106 98-99 0.05702586679202071 1106 100-101 0.08590764898838898 1107 1 0.12191394136850775 1107 2 0.1636571984593722 1107 3 0.2278505677992726 1107 4 0.1388866416079182 1107 5 0.14649057400865928 1107 6 0.1343992481392533 1107 7 0.27966337521597495 1107 8 -0.08210164530211017 1107 9 -0.004729762215497146 1107 10-11 0.07175073318848035 1107 12-13 0.021488378251781626 1107 14-15 -0.02050829917232022 1107 16-17 0.009022255491565545 1107 18-19 0.10342345230177585 1107 20-21 0.15884542913364186 1107 22-23 0.09877067772022485 1107 24-25 0.09055859197328431 1107 26-27 0.13317621789619238 1107 28-29 0.03642189052577294 1107 30-31 0.15000817335194228 1107 32-33 0.13269693482138223 1107 34-35 0.22198901645872837 1107 36-37 0.40316675514306866 1107 38-39 0.2660941971018005 1107 40-41 -0.0364668593431432 1107 42-43 0.07724391172034473 1107 44-45 0.0328478293593264 1107 46-47 0.12415678496408589 1107 48-49 0.2282194044232284 1107 50-51 0.1878428917814361 1107 52-53 0.24580356367208367 1107 54-55 0.15147016813119052 1107 56-57 0.03212023028621047 1107 58-59 0.23722213361438094 1107 60-61 0.10886143197258491 1107 62-63 0.13330694254565145 1107 64-65 0.012453026182647875 1107 66-67 0.24078130492384275 1107 68-69 0.31610883530431266 1107 70-71 0.44165474820129447 1107 72-73 0.3945315923850856 1107 74-75 0.36535713083192434 1107 76-77 0.3699594865320748 1107 78-79 0.4050385101564231 1107 80-81 0.3849181559451722 1107 82-83 0.41032856871283485 1107 84-85 0.3794690885084506 1107 86-87 0.36309035499490605 1107 88-89 0.3867907120218135 1107 90-91 0.34047218853173433 1107 92-93 0.36423182586196035 1107 94-95 0.3698819228745087 1107 96-97 0.31644761293947354 1107 98-99 0.28040228402558043 1107 100-101 0.35180674195891015 1108 1 -0.17868866008664952 1108 2 -0.1050581312030836 1108 3 -0.014187792178621095 1108 4 -0.15598641292984894 1108 5 -0.04219202708259928 1108 6 0.09155290866731747 1108 7 0.06518183535830246 1108 8 0.15815699911185988 1108 9 0.19515185542654478 1108 10-11 0.16665648218149443 1108 12-13 0.2905962119569736 1108 14-15 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-0.6229496438844109 2215 14-15 -0.8835262990647124 2215 16-17 -0.8256634063042512 2215 18-19 -0.8039155283686554 2215 20-21 -3.7193445067493585 2215 22-23 -1.4595429276966883 2215 24-25 -1.0498285197612276 2215 26-27 -0.9421731506210307 2215 28-29 -0.8713274358580847 2215 30-31 -1.371259720386071 2215 32-33 -1.1886762986649515 2215 34-35 -1.289393675565762 2215 36-37 -1.3918632055248708 2215 38-39 -1.1272119389874788 2215 40-41 -1.3507296503457056 2215 42-43 -1.7505598836680747 2215 44-45 -1.1913362659489977 2215 46-47 -1.0503443968164135 2215 48-49 -1.025433689133827 2215 50-51 -0.9235799380909242 2215 52-53 -0.8121153571174347 2215 54-55 -0.7627244150527943 2215 56-57 -0.7042495160825055 2215 58-59 -0.5834728864381233 2215 60-61 -0.6718414830354646 2215 62-63 -0.699113058985084 2215 64-65 -0.6763313502168984 2215 66-67 -0.8183672952908125 2215 68-69 -0.6492015055339664 2215 70-71 -0.48792808340893856 2215 72-73 -0.5340484055789716 2215 74-75 -0.5069054565765114 2215 76-77 -0.5529379558357519 2215 78-79 -0.5494404341449339 2215 80-81 -0.6190626332068447 2215 82-83 -0.6233359587000038 2215 84-85 -0.7837361181964155 2215 86-87 -0.7623399080835505 2215 88-89 -0.6811459992562128 2215 90-91 -0.6687603437370484 2215 92-93 -0.6146857821749059 2215 94-95 -0.6346593626531067 2215 96-97 -0.6981557498493309 2215 98-99 -0.718535048138726 2215 100-101 -0.6868986027908477 2216 1 -0.5168393488923186 2216 2 -0.4901657712645324 2216 3 -0.5418288952757386 2216 4 -0.4812036055462201 2216 5 -0.4156648744353717 2216 6 -0.459037892211029 2216 7 -0.7035543004640132 2216 8 -1.629514446268388 2216 9 -1.0469995009317543 2216 10-11 -0.7864071195673574 2216 12-13 -0.7425634568876376 2216 14-15 -1.5873977350136599 2216 16-17 -1.0010727205222807 2216 18-19 -0.998181226420634 2216 20-21 -0.9949191420214518 2216 22-23 -0.8598372713220357 2216 24-25 -1.0314061068040488 2216 26-27 -1.3860437481471735 2216 28-29 -1.9301721340187186 2216 30-31 -1.1429868471018025 2216 32-33 -1.155251024932035 2216 34-35 -2.3932692476509345 2216 36-37 -2.2715033641609352 2216 38-39 -2.238003958073172 2216 40-41 -1.4595294512140669 2216 42-43 -1.3933203608898737 2216 44-45 -1.1935531665499255 2216 46-47 -1.5369318213327006 2216 48-49 -1.4548715036317859 2216 50-51 -0.9425705131115052 2216 52-53 -0.8502603727902738 2216 54-55 -0.7954557881809805 2216 56-57 -0.844939387530772 2216 58-59 -0.8401599582508439 2216 60-61 -0.9514731206089238 2216 62-63 -0.858059754887087 2216 64-65 -0.8898595571421382 2216 66-67 -0.8797618872273887 2216 68-69 -0.9715938504601169 2216 70-71 -1.0936806589934065 2216 72-73 -0.9547462594591636 2216 74-75 -0.8025760726002034 2216 76-77 -0.885482120596528 2216 78-79 -0.9198973938534252 2216 80-81 -1.0200502803405627 2216 82-83 -1.1981987618173662 2216 84-85 -0.8690367627432707 2216 86-87 -1.0200523995943662 2216 88-89 -0.8730421826100745 2216 90-91 -1.051632609836524 2216 92-93 -1.268182798279156 2216 94-95 -0.9206603576893073 2216 96-97 -0.8363956851837955 2216 98-99 -0.9108083040622503 2216 100-101 -1.0204510891467322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 54.0 11 938.0 12 4026.0 13 8111.0 14 11544.0 15 14013.0 16 15944.0 17 17762.0 18 22054.0 19 32084.0 20 31465.0 21 31062.0 22 34965.0 23 42787.0 24 50861.0 25 59351.0 26 70001.0 27 82974.0 28 98108.0 29 116491.0 30 135748.0 31 162430.0 32 199366.0 33 253699.0 34 334370.0 35 474051.0 36 768489.0 37 1269304.0 38 1173395.0 39 202656.0 40 138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.820180690258 40.624117740442365 11.778454987500247 19.77724658179939 2 24.363078470244105 27.462512183638733 31.07102123167993 17.103388114437237 3 22.79280601797603 27.78273039084545 23.500435761906203 25.924027829272315 4 25.58517250357662 25.728359711182847 25.279252675814224 23.407215109426314 5 23.18611236786976 29.02269198752502 24.329546961542487 23.46164868306273 6 22.68631440639708 25.085815143729544 26.087978603628226 26.13989184624515 7 26.009128231661805 26.068932439786046 24.416804020438033 23.505135308114113 8 25.518760592349548 24.736102924843944 26.201428229125312 23.5437082536812 9 22.385904702810347 25.566307668926935 25.92200591495456 26.12578171330816 10-11 25.18681630391747 25.297200999478154 25.054571521308162 24.461411175296213 12-13 24.738692197040386 25.49016881773374 24.80331033749469 24.967828647731192 14-15 24.548729833020747 25.478529783721722 25.03531071285013 24.937429670407408 16-17 24.627958012947694 26.986796109781352 24.914331142857094 23.470914734413864 18-19 23.287501381569097 25.528843665138428 26.282454632068912 24.90120032122356 20-21 23.09236845948217 27.28085324808081 25.194961799886844 24.43181649255017 22-23 22.597752867946557 26.87836670798871 27.134738282795816 23.389142141268916 24-25 22.604749099271967 25.52319013923634 28.457618467732086 23.414442293759606 26-27 22.5241087316126 25.468331503323576 29.199137825794498 22.808421939269326 28-29 22.574694251931056 25.406540291439512 29.327773159166703 22.69099229746273 30-31 22.66963320753661 25.36276148871502 29.188523701431563 22.779081602316804 32-33 22.57828472291449 25.350949910019573 29.32305685119213 22.747708515873807 34-35 22.60144765329071 25.443818181182255 29.35956468971113 22.5951694758159 36-37 22.625892332414114 25.37703801644972 29.17740633870695 22.81966331242921 38-39 22.714225786978776 25.458189360070932 28.955680414730445 22.87190443821985 40-41 23.150164393592213 25.91678250423018 27.613357953242286 23.31969514893532 42-43 23.171983931427796 26.063442022056734 27.52900276550757 23.235571281007907 44-45 23.222163578054396 26.29842909412948 27.254909058984957 23.224498268831162 46-47 23.180764854087123 26.647984931030365 27.08227932330939 23.088970891573123 48-49 23.18475209421918 26.702381029410965 26.85140412934677 23.261462747023078 50-51 23.150604232764167 26.73063559492843 26.89514136539702 23.22361880691038 52-53 23.198656125155033 26.81887594673188 26.78701217119277 23.195455756920317 54-55 23.25928174809531 26.601742005838165 26.7754634064487 23.36351283961782 56-57 23.198051638607048 26.532661005368606 26.92013155793888 23.349155798085462 58-59 23.240830791492574 26.684046380804094 26.84304514417619 23.23207768352714 60-61 23.280393393702713 26.586908456639023 26.726400653627575 23.406297496030685 62-63 23.27637117778002 26.582606434391277 26.80805163685826 23.332970750970446 64-65 23.328728086091306 26.788557068194674 26.6429933640719 23.239721481642114 66-67 23.42928763477411 26.682849425927675 26.50840719730273 23.379455741995482 68-69 23.369765282715434 26.7022498701961 26.543075047029323 23.384909800059145 70-71 23.388011302552254 26.84741773056188 26.46817102127382 23.29639994561204 72-73 23.43788160231854 26.752859987095313 26.358837626603982 23.450420783982164 74-75 23.421439869156224 26.76571590866959 26.461245646177556 23.351598575996633 76-77 23.463957290850367 26.90593707476182 26.327538828765196 23.302566805622618 78-79 23.529563498100988 26.80744614508605 26.262680051218613 23.40031030559435 80-81 23.443008749643244 26.777857134862586 26.37551729782364 23.40361681767053 82-83 23.530118488708474 26.908587379695152 26.267929212466477 23.293364919129893 84-85 23.586842527273685 26.83847183076054 26.173617026634588 23.401068615331184 86-87 23.557366679718466 26.783192593666477 26.297090311513628 23.362350415101428 88-89 23.611948465616305 26.89684877717193 26.197053097014724 23.294149660197046 90-91 23.68370797942934 26.813700227045345 26.14129065214285 23.361301141382462 92-93 23.658516666226554 26.76924599715192 26.280677921759505 23.291559414862018 94-95 23.707613932326392 26.92214266590023 26.151223776682375 23.219019625091004 96-97 23.767457510097948 26.845475732834622 26.11741967503643 23.269647082030996 98-99 23.758416268219545 26.823624607637207 26.18248338955983 23.235475734583414 100-101 23.85679442332004 26.948531899932167 26.032953140659863 23.161720536087934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1837.0 1 1221.5 2 495.5 3 329.5 4 233.5 5 161.5 6 120.0 7 110.5 8 103.0 9 89.5 10 83.5 11 89.0 12 105.0 13 128.0 14 165.5 15 251.0 16 1100.5 17 1456.0 18 1181.0 19 1732.5 20 2689.0 21 4000.5 22 5615.0 23 7717.0 24 10468.0 25 14184.0 26 18851.5 27 24337.0 28 31020.5 29 38732.5 30 47799.0 31 57641.0 32 67217.5 33 77381.0 34 87339.0 35 98544.0 36 112208.0 37 126450.5 38 141390.5 39 156741.5 40 171207.5 41 183238.5 42 195108.5 43 207460.5 44 217989.0 45 227204.0 46 235013.0 47 239374.5 48 240032.0 49 238673.5 50 235889.0 51 232857.0 52 230320.5 53 224736.5 54 213599.0 55 198607.5 56 180309.5 57 159938.5 58 141140.0 59 122584.5 60 101693.0 61 81821.0 62 66252.5 63 53402.0 64 41901.0 65 32450.0 66 24834.5 67 18646.5 68 14074.5 69 11134.5 70 8877.0 71 7229.5 72 5939.5 73 4689.5 74 3564.5 75 2395.5 76 1427.5 77 894.0 78 574.0 79 316.5 80 157.5 81 86.5 82 48.5 83 31.0 84 22.5 85 12.5 86 7.5 87 8.5 88 6.5 89 4.0 90 3.5 91 2.5 92 2.5 93 1.5 94 2.5 95 6.5 96 4.5 97 1.5 98 2.0 99 2.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08618034811754174 2 0.045188721496698024 3 0.05183412171680067 4 0.05849700983221938 5 0.043352492488511765 6 0.017662774269220204 7 0.021894844935706627 8 0.011734377757075996 9 0.0033576759006834446 10-11 9.968100330153976E-4 12-13 5.683565977719372E-4 14-15 0.0013203360963625003 16-17 3.497579063211921E-5 18-19 0.0018362290081862587 20-21 0.0057010538730354315 22-23 0.016674708183862835 24-25 0.020967986483955467 26-27 0.010545200875583943 28-29 0.018082483756805632 30-31 3.6724580163725174E-4 32-33 0.00578849334961573 34-35 3.6724580163725174E-4 36-37 0.002631928245066971 38-39 0.010483993241977733 40-41 0.001827485060528229 42-43 0.002142267176217302 44-45 0.0024308174489322855 46-47 0.0 48-49 0.0 50-51 0.0035762745921341893 52-53 0.0026056964020928814 54-55 0.00310410141860058 56-57 0.0 58-59 0.004756707525968212 60-61 0.0 62-63 0.0 64-65 0.0022034748098235107 66-67 2.710623773989239E-4 68-69 0.0 70-71 0.001110481352569785 72-73 0.0028767587794918054 74-75 0.004844147002548511 76-77 0.002133523228559272 78-79 1.2241526721241725E-4 80-81 0.0011367131955438744 82-83 0.0010317858236475169 84-85 0.0 86-87 0.0 88-89 8.743947658029803E-6 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5718241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.7705353678547 #Duplication Level Percentage of deduplicated Percentage of total 1 82.28722191827185 56.58936305253023 2 10.341349094360917 14.223602272901594 3 3.1816577918798488 6.56412929114651 4 1.391522577939135 3.827830106453265 5 0.791415830316651 2.7213045174735675 6 0.46670219836565585 1.925721602337653 7 0.30351431354567027 1.4610989284039861 8 0.22883971398981723 1.2589943715605183 9 0.1755478290605822 1.086526636844479 >10 0.8209677701993527 9.333056926333915 >50 0.009920368101548352 0.4270974539351745 >100 0.0010343807439217997 0.13903096417361088 >500 1.2758943296673584E-4 0.07116015921786673 >1k 1.5310610750422974E-4 0.17552158023905795 >5k 0.0 0.0 >10k+ 2.5517684554286927E-5 0.19556213644862913 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 11144 0.19488510540216825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.497579063211921E-5 0.0 0.0 0.0 0.0 2 3.497579063211921E-5 0.0 0.0 0.0 0.0 3 5.246368594817882E-5 0.0 0.0 0.0 0.0 4 6.995158126423842E-5 0.0 0.0 0.0 0.0 5 1.0492737189635764E-4 0.0 0.0 0.0 0.0 6 1.9236684847665568E-4 0.0 0.0 0.0 0.0 7 2.623184297408941E-4 0.0 0.0 0.0 0.0 8 3.8473369695331137E-4 0.0 1.7487895316059606E-5 0.0 0.0 9 4.89661068849669E-4 0.0 1.7487895316059606E-5 0.0 0.0 10-11 8.219310798548015E-4 0.0 2.623184297408941E-5 0.0 0.0 12-13 0.001381543729968709 0.0 6.120763360620862E-5 0.0 0.0 14-15 0.0018449729558442885 0.0 8.743947658029803E-5 0.0 0.0 16-17 0.0022909142864038085 0.0 1.0492737189635764E-4 0.0 0.0 18-19 0.002701879826331209 0.0 1.5739105784453645E-4 8.743947658029803E-6 0.0 20-21 0.0031915408951808783 0.0 2.3608658676680468E-4 3.497579063211921E-5 0.0 22-23 0.003681201964030547 1.7487895316059606E-5 3.847336969533113E-4 4.371973829014902E-5 0.0 24-25 0.00443318146262111 1.7487895316059606E-5 5.858444930879968E-4 5.246368594817882E-5 0.0 26-27 0.005150185170579554 1.7487895316059606E-5 6.995158126423843E-4 5.246368594817882E-5 0.0 28-29 0.0058409570355639085 1.7487895316059606E-5 7.956992368807121E-4 5.246368594817882E-5 0.0 30-31 0.0065667046911803825 1.7487895316059606E-5 9.181145040931294E-4 5.246368594817882E-5 0.0 32-33 0.007650954200776078 1.7487895316059606E-5 0.001023041875989487 5.246368594817882E-5 0.0 34-35 0.009224864779221444 2.623184297408941E-5 0.0010492737189635765 5.246368594817882E-5 0.0 36-37 0.011244716688226327 3.497579063211921E-5 0.0010492737189635765 5.246368594817882E-5 0.0 38-39 0.014366306002142967 3.497579063211921E-5 0.0010492737189635765 5.246368594817882E-5 0.0 40-41 0.01819615507636002 3.497579063211921E-5 0.0010667616142796361 6.995158126423842E-5 0.0 42-43 0.02349498735712608 3.497579063211921E-5 0.0010929934572537253 6.995158126423842E-5 0.0 44-45 0.030420193902285682 3.497579063211921E-5 0.0011017374049117552 6.995158126423842E-5 0.0 46-47 0.03915539761265746 3.497579063211921E-5 0.001110481352569785 6.995158126423842E-5 0.0 48-49 0.04940330426786839 3.497579063211921E-5 0.0011192253002278148 6.995158126423842E-5 1.7487895316059606E-5 50-51 0.06251048180725506 3.497579063211921E-5 0.0011279692478858446 6.995158126423842E-5 1.7487895316059606E-5 52-53 0.07769871888925284 3.497579063211921E-5 0.0011367131955438744 6.995158126423842E-5 1.7487895316059606E-5 54-55 0.09519535815297046 3.497579063211921E-5 0.0011367131955438744 6.995158126423842E-5 1.7487895316059606E-5 56-57 0.11726508204183769 3.497579063211921E-5 0.0011367131955438744 7.869552892226823E-5 1.7487895316059606E-5 58-59 0.1427624334126526 3.497579063211921E-5 0.0011367131955438744 9.618342423832783E-5 1.7487895316059606E-5 60-61 0.17132891041143597 3.497579063211921E-5 0.0011367131955438744 1.3115921487044706E-4 1.7487895316059606E-5 62-63 0.20575383234109929 3.497579063211921E-5 0.0011367131955438744 1.4864711018650664E-4 1.7487895316059606E-5 64-65 0.24505787706394327 5.246368594817882E-5 0.0011367131955438744 1.6613500550256627E-4 1.7487895316059606E-5 66-67 0.28904867773149123 6.120763360620862E-5 0.0011367131955438744 1.7487895316059605E-4 1.7487895316059606E-5 68-69 0.33909029017839576 6.995158126423842E-5 0.0011367131955438744 1.8362290081862587E-4 1.7487895316059606E-5 70-71 0.39422088016227363 6.995158126423842E-5 0.0011367131955438744 1.9236684847665568E-4 1.7487895316059606E-5 72-73 0.4566264345976324 6.995158126423842E-5 0.0011367131955438744 1.9236684847665568E-4 1.7487895316059606E-5 74-75 0.5268578221869278 6.995158126423842E-5 0.0011367131955438744 2.273426391087749E-4 1.7487895316059606E-5 76-77 0.6060080363874135 6.995158126423842E-5 0.0011367131955438744 2.535744820828643E-4 1.7487895316059606E-5 78-79 0.6923807513534319 6.995158126423842E-5 0.0011367131955438744 2.623184297408941E-4 1.7487895316059606E-5 80-81 0.7871126802805268 6.995158126423842E-5 0.0011367131955438744 2.623184297408941E-4 1.7487895316059606E-5 82-83 0.8909995224055789 6.995158126423842E-5 0.0011367131955438744 2.7106237739892393E-4 1.7487895316059606E-5 84-85 1.0056676519929817 6.995158126423842E-5 0.0011367131955438744 2.972942203730133E-4 1.7487895316059606E-5 86-87 1.1308372627176784 6.995158126423842E-5 0.0011367131955438744 2.972942203730133E-4 1.7487895316059606E-5 88-89 1.2661148769350574 6.995158126423842E-5 0.0011367131955438744 2.972942203730133E-4 1.7487895316059606E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 16315 0.0 91.12779 1 TATCTCG 7215 0.0 84.053024 2 TCGACGG 18085 0.0 82.492 2 CGACGGC 19065 0.0 78.1755 3 ACGGCGC 19125 0.0 78.06363 5 CGGCGCG 19160 0.0 77.80277 6 GACGGCG 19265 0.0 77.48184 4 ATCTCGA 8230 0.0 73.57004 3 ATATCTC 8600 0.0 70.876076 1 TCTCGAG 7050 0.0 67.34066 4 TCGAGGG 7065 0.0 67.10518 6 AGGGCGC 7220 0.0 65.71655 9 CTCGAGG 7325 0.0 64.99861 5 CGAGGGC 7610 0.0 62.421474 7 GAGGGCG 7935 0.0 59.68001 8 CGCGCCG 25300 0.0 59.321625 9 GGCGCGC 25405 0.0 59.10496 7 GCGCGCC 25460 0.0 58.99078 8 CTCGACG 2330 0.0 56.081646 5 TCTCGAC 2365 0.0 55.66082 4 >>END_MODULE