FastQCFastQC Report
Tue 31 May 2016
SRR2048240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5718241
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT216020.3777735146175196No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT126830.221798976293584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG37350.076.7190251
TCGACGG44300.065.97022
CGACGGC54500.053.6234783
ATCTCGA83650.051.638581
ACGGCGC58150.050.234755
TATCTCG40800.049.1505852
CGGCGCG59750.048.9690486
ATATCTC42150.048.5910221
GACGGCG60250.048.505894
TCTCGAG86350.047.272352
TCGAGGG94800.043.0086024
CTCGAGG96500.042.3494263
GGGCGCG99450.040.8834768
CGAGGGC101100.040.3102045
AGGGCGC102800.039.5973937
GAGGGCG107100.038.007586
GGCGCGC157650.027.3571459
CGCGCCG155650.024.9316969
GCGCGCC158100.024.6354718
TCCATAT81250.020.19777518-19
Produced by FastQC (version 0.11.5)