##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048240_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5718241 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28247497788218 33.0 31.0 34.0 30.0 34.0 2 32.410990197859796 34.0 31.0 34.0 31.0 34.0 3 32.48983769659236 34.0 31.0 34.0 31.0 34.0 4 36.04950001232896 37.0 35.0 37.0 35.0 37.0 5 35.91135280936918 37.0 35.0 37.0 35.0 37.0 6 35.903583811875016 37.0 35.0 37.0 35.0 37.0 7 35.88116782765889 37.0 35.0 37.0 35.0 37.0 8 35.85430904363772 37.0 35.0 37.0 35.0 37.0 9 37.456723492416636 39.0 37.0 39.0 34.0 39.0 10-11 37.448240639035674 39.0 37.0 39.0 34.0 39.0 12-13 37.44701150231339 39.0 37.0 39.0 34.0 39.0 14-15 38.78479089985889 40.0 38.0 41.0 34.0 41.0 16-17 38.79554009703334 40.0 38.0 41.0 34.5 41.0 18-19 38.83090455613886 40.0 38.0 41.0 35.0 41.0 20-21 38.851022980668354 40.0 38.0 41.0 35.0 41.0 22-23 38.83262807566173 40.0 38.0 41.0 35.0 41.0 24-25 38.81850231565966 40.0 38.0 41.0 35.0 41.0 26-27 38.73475855599651 40.0 38.0 41.0 34.0 41.0 28-29 38.653442028763735 40.0 38.0 41.0 34.0 41.0 30-31 38.53444328421975 40.0 38.0 41.0 34.0 41.0 32-33 38.446880955174855 40.0 38.0 41.0 34.0 41.0 34-35 38.405498386654216 40.0 38.0 41.0 34.0 41.0 36-37 38.33150815084569 40.0 38.0 41.0 34.0 41.0 38-39 38.2512785837463 40.0 38.0 41.0 33.5 41.0 40-41 38.13149200951831 40.0 37.5 41.0 33.0 41.0 42-43 38.06304972105933 40.0 37.0 41.0 33.0 41.0 44-45 37.95246632662037 40.0 37.0 41.0 33.0 41.0 46-47 37.83618642516116 40.0 37.0 41.0 33.0 41.0 48-49 37.73592359958246 40.0 37.0 41.0 33.0 41.0 50-51 37.65361087439302 40.0 37.0 41.0 32.5 41.0 52-53 37.63451706565009 40.0 36.5 41.0 32.5 41.0 54-55 37.472384514748505 39.5 36.0 41.0 32.0 41.0 56-57 37.25743169971325 39.0 36.0 41.0 32.0 41.0 58-59 37.02989179714531 39.0 35.0 41.0 31.5 41.0 60-61 36.78304237264571 39.0 35.0 41.0 31.0 41.0 62-63 36.492314769524405 38.0 35.0 40.0 31.0 41.0 64-65 36.19329291997312 37.5 35.0 40.0 31.0 41.0 66-67 35.86477362531589 37.0 35.0 40.0 31.0 41.0 68-69 35.51777565863348 36.5 34.5 39.0 30.0 41.0 70-71 35.16347605845924 36.0 34.0 39.0 30.0 41.0 72-73 34.79235345274884 35.5 34.0 38.5 30.0 40.0 74-75 34.44631076234808 35.0 34.0 37.0 30.0 39.5 76-77 34.190615173442325 35.0 34.0 37.0 29.5 39.0 78-79 34.02464866381112 35.0 34.0 36.5 30.0 39.0 80-81 33.771495202808 35.0 34.0 36.0 30.0 37.5 82-83 33.532495482439444 35.0 34.0 36.0 30.0 37.0 84-85 33.33012188538399 35.0 34.0 35.0 30.0 37.0 86-87 33.130392370660836 35.0 34.0 35.0 29.0 36.0 88-89 32.993859125559766 35.0 34.0 35.0 29.0 36.0 90-91 32.86649513373081 35.0 34.0 35.0 29.0 36.0 92-93 32.73773482090034 35.0 33.5 35.0 29.0 36.0 94-95 32.62991757430301 35.0 33.0 35.0 29.0 35.0 96-97 32.52237864755962 35.0 33.0 35.0 29.0 35.0 98-99 32.382464817415006 35.0 33.0 35.0 29.0 35.0 100-101 31.939907394599143 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3627803175729767 1101 2 0.3828938584321264 1101 3 0.3080770039229037 1101 4 0.2711557704293739 1101 5 0.2669293539041746 1101 6 0.3057755764684842 1101 7 0.2769894095374923 1101 8 0.34644953108433185 1101 9 0.42359130684644697 1101 10-11 0.39831472149501224 1101 12-13 0.38139538019744634 1101 14-15 0.4886270623264508 1101 16-17 0.47573266811968296 1101 18-19 0.5408769428740996 1101 20-21 0.5285304760911416 1101 22-23 0.5709417884695256 1101 24-25 0.5358409182150794 1101 26-27 0.5735785329567165 1101 28-29 0.5355560367715029 1101 30-31 0.5179558302028866 1101 32-33 0.5212775284729219 1101 34-35 0.496533328296465 1101 36-37 0.5619010134386784 1101 38-39 0.5175586876482896 1101 40-41 0.5297034397874754 1101 42-43 0.41488792161707977 1101 44-45 0.5044756554271146 1101 46-47 0.528484185842423 1101 48-49 0.5365243707538525 1101 50-51 0.49793027947887936 1101 52-53 0.49372323636508497 1101 54-55 0.4631388238974239 1101 56-57 0.4568935963860099 1101 58-59 0.4618769163937202 1101 60-61 0.4678291370921528 1101 62-63 0.46301300197957573 1101 64-65 0.45841248181304906 1101 66-67 0.48982176241564446 1101 68-69 0.4597570358557448 1101 70-71 0.39861561773573584 1101 72-73 0.40273769027714224 1101 74-75 0.36942844004997966 1101 76-77 0.4250183223410744 1101 78-79 0.39449146407886104 1101 80-81 0.3472743341659097 1101 82-83 0.3715554170342159 1101 84-85 0.3708730816218946 1101 86-87 0.38982860141698694 1101 88-89 0.3534776407974718 1101 90-91 0.3704778981865573 1101 92-93 0.4008882082504499 1101 94-95 0.3808280232276715 1101 96-97 0.474147694741319 1101 98-99 0.49596926385351736 1101 100-101 0.5591041111285477 1102 1 0.31248602789069224 1102 2 0.317548488365361 1102 3 0.29882717367586054 1102 4 0.23293120377427812 1102 5 0.24133238268458257 1102 6 0.23753331059099736 1102 7 0.22976815138993345 1102 8 0.2432900996084868 1102 9 0.3085431647894197 1102 10-11 0.34548976143187815 1102 12-13 0.3487056471274883 1102 14-15 0.43221732227498677 1102 16-17 0.4437329502319116 1102 18-19 0.4555509051168798 1102 20-21 0.4634089229756668 1102 22-23 0.4525888271497607 1102 24-25 0.4436571028423586 1102 26-27 0.4634539540196556 1102 28-29 0.46769323314074285 1102 30-31 0.4302276175424069 1102 32-33 0.4569703879314915 1102 34-35 0.43093721685525566 1102 36-37 0.43649913978568833 1102 38-39 0.4400149761341865 1102 40-41 0.45557906854139674 1102 42-43 0.4313938019397838 1102 44-45 0.4560963602183534 1102 46-47 0.4180834778899296 1102 48-49 0.4342308647894839 1102 50-51 0.40051712232062187 1102 52-53 0.4224216265591565 1102 54-55 0.40029263042278274 1102 56-57 0.4021573915977825 1102 58-59 0.38430558378190227 1102 60-61 0.32076381930220066 1102 62-63 0.31604036239806277 1102 64-65 0.31658625925265227 1102 66-67 0.3464275507895991 1102 68-69 0.3233814926903449 1102 70-71 0.24057072918993327 1102 72-73 0.2490941252982779 1102 74-75 0.28986281500605315 1102 76-77 0.2686023818859269 1102 78-79 0.24381694170972423 1102 80-81 0.24853003680586738 1102 82-83 0.22751872802027862 1102 84-85 0.2053905363320041 1102 86-87 0.22926918894805937 1102 88-89 0.1924048201502373 1102 90-91 0.13074992419858233 1102 92-93 0.171705264499586 1102 94-95 0.13197844809466375 1102 96-97 0.18225315914111206 1102 98-99 0.2684609618490228 1102 100-101 0.2501736897801763 1103 1 0.2897860216972745 1103 2 0.28395473457551645 1103 3 0.2530343382504654 1103 4 0.17577184365241294 1103 5 0.22154341652512954 1103 6 0.21968858532907376 1103 7 0.2323242760168398 1103 8 0.2227264659379955 1103 9 0.36989727779364046 1103 10-11 0.317360108517029 1103 12-13 0.33894474108107886 1103 14-15 0.461961825510528 1103 16-17 0.40087120382531083 1103 18-19 0.4148403529693212 1103 20-21 0.46150745414161776 1103 22-23 0.4914057513363588 1103 24-25 0.48770043590116785 1103 26-27 0.44559548875304955 1103 28-29 0.4003729816625139 1103 30-31 0.4432846734460014 1103 32-33 0.46900358840535716 1103 34-35 0.47587992560452363 1103 36-37 0.4649162443486574 1103 38-39 0.47620561096580616 1103 40-41 0.5134391151515203 1103 42-43 0.46687654097995335 1103 44-45 0.49090005411749615 1103 46-47 0.4687015140507427 1103 48-49 0.49775018081057 1103 50-51 0.5758859016947468 1103 52-53 0.5554706674376249 1103 54-55 0.4851031150420937 1103 56-57 0.419792993316527 1103 58-59 0.37719586035079544 1103 60-61 0.3748533264362024 1103 62-63 0.43370296977687417 1103 64-65 0.4040606049170137 1103 66-67 0.40971408627166994 1103 68-69 0.3641346287741811 1103 70-71 0.35929245241147356 1103 72-73 0.3610769372444622 1103 74-75 0.3208800659615463 1103 76-77 0.2822802441234131 1103 78-79 0.2807012485724485 1103 80-81 0.2824258556962391 1103 82-83 0.24754480164441617 1103 84-85 0.2600267276817263 1103 86-87 0.2871084810963467 1103 88-89 0.2739400168754571 1103 90-91 0.274595733100071 1103 92-93 0.2818672671502185 1103 94-95 0.2508239657349307 1103 96-97 0.2876987657682264 1103 98-99 0.3053807710025751 1103 100-101 0.36307370246935733 1104 1 0.16206257842935656 1104 2 0.1830541256878817 1104 3 0.16257708298439866 1104 4 0.11219593220744883 1104 5 0.13884769930903218 1104 6 0.16273248716986188 1104 7 0.1576613809634395 1104 8 0.1740965082103827 1104 9 0.1856619219539084 1104 10-11 0.19943084767253083 1104 12-13 0.186177264133363 1104 14-15 0.25369147226902555 1104 16-17 0.2074445924770103 1104 18-19 0.2713498961505536 1104 20-21 0.29766572268874114 1104 22-23 0.3308556562117957 1104 24-25 0.26443787812456065 1104 26-27 0.2419574901266941 1104 28-29 0.25465388625898555 1104 30-31 0.26544496014408736 1104 32-33 0.2979520810952607 1104 34-35 0.1685199593975426 1104 36-37 0.29140496851952236 1104 38-39 0.28518936118075544 1104 40-41 0.28317762852765327 1104 42-43 0.2599323734145642 1104 44-45 0.26774064912544304 1104 46-47 0.3013899257547976 1104 48-49 0.23921678731501572 1104 50-51 0.2656244971435342 1104 52-53 0.3306368756779676 1104 54-55 0.3191447846372739 1104 56-57 0.28726217679786714 1104 58-59 0.2734526950232379 1104 60-61 0.25128928102472514 1104 62-63 0.2528212817733575 1104 64-65 0.18936317191188579 1104 66-67 0.19213908525817658 1104 68-69 0.2280893120687324 1104 70-71 0.22849018669381138 1104 72-73 0.16841522868985948 1104 74-75 0.13988608348176257 1104 76-77 0.1647706320168254 1104 78-79 0.12484233769177422 1104 80-81 0.11554896497025169 1104 82-83 0.13989923827143258 1104 84-85 0.1775782151401728 1104 86-87 0.16736525115617695 1104 88-89 0.12678685207144014 1104 90-91 0.15758413082301814 1104 92-93 0.18003977305559715 1104 94-95 0.1650970229829838 1104 96-97 0.12684460974345058 1104 98-99 0.13957479777023707 1104 100-101 0.11717845927774562 1105 1 0.15340301971600923 1105 2 0.19521448767612526 1105 3 0.16694723886538299 1105 4 0.06546792658078715 1105 5 0.14298653162149577 1105 6 0.14959970991750993 1105 7 0.1273368702853901 1105 8 0.12130018565933653 1105 9 0.17288983821318027 1105 10-11 0.20407764271015338 1105 12-13 0.20086529197162406 1105 14-15 0.24391229355667576 1105 16-17 0.210080285840327 1105 18-19 0.18714477721711376 1105 20-21 0.15689636871570656 1105 22-23 0.1876606802842531 1105 24-25 0.21805899854151534 1105 26-27 0.22370565048662172 1105 28-29 0.1278214097608057 1105 30-31 0.18224894482086995 1105 32-33 0.18927884001452355 1105 34-35 0.19769944549247498 1105 36-37 0.19586427256803773 1105 38-39 0.21588415979204 1105 40-41 0.2706620279207286 1105 42-43 0.21466341624307717 1105 44-45 0.22524861933012374 1105 46-47 0.17808216121118647 1105 48-49 0.23717521303549916 1105 50-51 0.2082020643025544 1105 52-53 0.25661733142846543 1105 54-55 0.2203613934064208 1105 56-57 0.20829789461149062 1105 58-59 0.13575203427193117 1105 60-61 0.19580000807813036 1105 62-63 0.09927184870824846 1105 64-65 0.10617571935204495 1105 66-67 0.1687593238652525 1105 68-69 0.06767862955717163 1105 70-71 0.1487896487098368 1105 72-73 0.08956800102635043 1105 74-75 -0.00636193025373899 1105 76-77 0.11460183983329131 1105 78-79 0.09720937177491606 1105 80-81 0.1295945921191688 1105 82-83 0.14133381063491157 1105 84-85 0.1698428158768266 1105 86-87 0.18625754423067775 1105 88-89 0.12097007921705227 1105 90-91 0.09073764182681998 1105 92-93 0.08885326807384786 1105 94-95 0.023866427507265087 1105 96-97 0.07905123644539458 1105 98-99 0.0894778992701859 1105 100-101 0.026864982184555686 1106 1 0.06556459011994065 1106 2 0.08505404977503161 1106 3 0.05250367525647448 1106 4 0.06357878602459976 1106 5 0.03860928234504257 1106 6 0.039682005123253816 1106 7 0.05165938192499908 1106 8 0.09860633892472492 1106 9 0.14013068381529337 1106 10-11 0.13796974035706455 1106 12-13 0.1337179407699196 1106 14-15 0.18792449942052514 1106 16-17 0.1823301285482657 1106 18-19 0.18181100346601653 1106 20-21 0.17563664806640844 1106 22-23 0.18752987620725037 1106 24-25 0.17051759927135635 1106 26-27 0.15593504522676938 1106 28-29 0.14970734155896537 1106 30-31 0.1343446413101077 1106 32-33 0.16635377395185458 1106 34-35 0.15130181116505526 1106 36-37 0.11492555355457057 1106 38-39 0.11542002238169857 1106 40-41 0.21880082243438181 1106 42-43 0.15460823818846592 1106 44-45 0.1582276680277488 1106 46-47 0.10595462880902318 1106 48-49 0.05887440770014507 1106 50-51 0.1084561224377012 1106 52-53 -0.04098622966377263 1106 54-55 0.008672328350428415 1106 56-57 0.02440221070560966 1106 58-59 -0.024064689019880348 1106 60-61 -0.11817488810910959 1106 62-63 -0.019709842495977625 1106 64-65 -0.03005084413103276 1106 66-67 0.0211538123514714 1106 68-69 0.02487417504603684 1106 70-71 0.019550942279742856 1106 72-73 0.006449528646832903 1106 74-75 -0.04929366549974645 1106 76-77 0.01241434769789862 1106 78-79 0.026639093682561565 1106 80-81 -0.0502242074278314 1106 82-83 -0.005088109586559142 1106 84-85 1.426145429945791E-4 1106 86-87 -0.01412889090207159 1106 88-89 0.027187456843833502 1106 90-91 0.03586542589627584 1106 92-93 0.08538340523251975 1106 94-95 0.08574650804080619 1106 96-97 0.07146642456670094 1106 98-99 0.11913840008810439 1106 100-101 0.1074839578986655 1107 1 0.010929669485015836 1107 2 0.10508806338047094 1107 3 0.07619755280749274 1107 4 0.07557198330351156 1107 5 0.12377481522486278 1107 6 0.1130334790461518 1107 7 0.11067752251457108 1107 8 0.07228887860726019 1107 9 0.16169757723933031 1107 10-11 0.05260352720513595 1107 12-13 0.09967032172230006 1107 14-15 0.10783379647267566 1107 16-17 0.0015852305890788898 1107 18-19 0.028041162196174696 1107 20-21 0.014498326070942369 1107 22-23 0.09885300545894893 1107 24-25 0.07881578521239163 1107 26-27 4.7926291384925435E-4 1107 28-29 0.08988307852041544 1107 30-31 0.12989226035772816 1107 32-33 0.08842066737589249 1107 34-35 0.05604330776368727 1107 36-37 0.031104691876571167 1107 38-39 0.08433272079439291 1107 40-41 0.1488416387609135 1107 42-43 0.1259245647190781 1107 44-45 0.140816103401896 1107 46-47 0.1119466923010819 1107 48-49 0.11719526937815772 1107 50-51 0.15226111109983265 1107 52-53 0.18807612861287737 1107 54-55 0.14439568435949468 1107 56-57 0.2049736392770427 1107 58-59 0.04921308875789521 1107 60-61 0.08165617765052957 1107 62-63 -0.13293546607874873 1107 64-65 -0.007199370208120115 1107 66-67 0.02169349489115291 1107 68-69 0.06716669205283665 1107 70-71 0.08985706472871868 1107 72-73 0.03349601390760881 1107 74-75 0.03429023472700976 1107 76-77 0.009641105067508704 1107 78-79 -0.07043346867571643 1107 80-81 -0.027299037359803435 1107 82-83 0.07443569993724708 1107 84-85 0.08522764855659659 1107 86-87 -0.03768217888393366 1107 88-89 -0.005586919573403293 1107 90-91 -0.03304954009011851 1107 92-93 0.00902286101484151 1107 94-95 -0.037967777673479475 1107 96-97 0.027032184203889642 1107 98-99 -0.05611330059216613 1107 100-101 -0.010910599924464748 1108 1 -0.01729866631603727 1108 2 0.029827815516696887 1108 3 0.03185249950700353 1108 4 0.018328256658726616 1108 5 -0.02862865368527423 1108 6 0.007289717148211139 1108 7 0.015788547198596348 1108 8 0.0071800600882099275 1108 9 -0.18026572764129867 1108 10-11 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88-89 -0.2086890863660429 2214 90-91 -0.2554560026751531 2214 92-93 -0.19252664767753203 2214 94-95 -0.16327641964878836 2214 96-97 -0.19033207631512994 2214 98-99 -0.3200494305997239 2214 100-101 -0.5406646311665888 2215 1 -0.5563508233441006 2215 2 -0.9390674087671691 2215 3 -0.5776044157910185 2215 4 -0.3061964791592686 2215 5 -0.35039924562148883 2215 6 -0.323291222755401 2215 7 -0.3506567717021696 2215 8 -0.4226329763624932 2215 9 -0.3892554882540722 2215 10-11 -0.5235624406487176 2215 12-13 -0.4186030523854285 2215 14-15 -0.547635945172452 2215 16-17 -0.4798586811258403 2215 18-19 -0.5078011844710559 2215 20-21 -0.5057107582683003 2215 22-23 -0.5365990644187946 2215 24-25 -0.5363144468217484 2215 26-27 -0.49855746031207104 2215 28-29 -0.46891398083282354 2215 30-31 -0.5264562387166265 2215 32-33 -0.578464780873432 2215 34-35 -0.6481917340678223 2215 36-37 -0.5794739612548838 2215 38-39 -0.5692700930986021 2215 40-41 -0.6139819805312143 2215 42-43 -0.5640491764390347 2215 44-45 -0.5162058286860827 2215 46-47 -0.5633372343474363 2215 48-49 -0.6612006792504559 2215 50-51 -0.6601761971939482 2215 52-53 -0.6305731431340007 2215 54-55 -0.6483187847410505 2215 56-57 -0.5332969587768943 2215 58-59 -0.5525491307413191 2215 60-61 -0.4931657722203582 2215 62-63 -0.5500756848338 2215 64-65 -0.4849534037587162 2215 66-67 -0.4490503018663432 2215 68-69 -0.3616379856609555 2215 70-71 -0.3638264542768965 2215 72-73 -0.3723895106263697 2215 74-75 -0.4316059759721895 2215 76-77 -0.33963909752631594 2215 78-79 -0.3015803312676226 2215 80-81 -0.3224175905179507 2215 82-83 -0.3813677996587259 2215 84-85 -0.38728708233668385 2215 86-87 -0.3925849576405298 2215 88-89 -0.3677933026279092 2215 90-91 -0.3375843717347067 2215 92-93 -0.33817340043133015 2215 94-95 -0.37129319836940766 2215 96-97 -0.39739098263441974 2215 98-99 -0.43662420310771566 2215 100-101 -0.45741090923090866 2216 1 -0.4693566829118865 2216 2 -0.502949858656013 2216 3 -0.42527466086859533 2216 4 -0.2855060827637885 2216 5 -0.329476703137928 2216 6 -0.32902374585385985 2216 7 -0.31786769791866476 2216 8 -0.4298448056872246 2216 9 -0.49741202975254595 2216 10-11 -0.6201243411616417 2216 12-13 -0.6329989771530293 2216 14-15 -0.8432005703728933 2216 16-17 -1.1636684755835063 2216 18-19 -1.1800329384658639 2216 20-21 -1.60553978967787 2216 22-23 -1.376739526361149 2216 24-25 -1.4251219875516483 2216 26-27 -1.2536858369619495 2216 28-29 -1.2499618376705186 2216 30-31 -1.2571899762219942 2216 32-33 -1.0402450535188308 2216 34-35 -0.6432453691811233 2216 36-37 -0.9792899685001473 2216 38-39 -1.2635076716439855 2216 40-41 -1.3033806404355275 2216 42-43 -0.7113322978249315 2216 44-45 -1.1394152688790058 2216 46-47 -0.8222321383601567 2216 48-49 -0.8755228563981206 2216 50-51 -1.0488063007895505 2216 52-53 -1.0086068326788507 2216 54-55 -1.1552701618784909 2216 56-57 -1.1844436854417921 2216 58-59 -0.874083952001456 2216 60-61 -0.9294253068695824 2216 62-63 -0.8306715957064768 2216 64-65 -0.9125731780227042 2216 66-67 -0.7035737141700196 2216 68-69 -0.6728918774386088 2216 70-71 -0.617729906408151 2216 72-73 -0.6210197622520965 2216 74-75 -0.613553576164179 2216 76-77 -0.5636314367800495 2216 78-79 -0.4772045690756741 2216 80-81 -0.46526608347473797 2216 82-83 -0.7043929672080012 2216 84-85 -0.5620307522911787 2216 86-87 -0.6116649043024083 2216 88-89 -0.5689619010567881 2216 90-91 -0.608560313913884 2216 92-93 -0.5462609275424626 2216 94-95 -0.6647185952534471 2216 96-97 -0.6265768473484314 2216 98-99 -0.86139537992306 2216 100-101 -0.6618275998902803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 15.0 12 50.0 13 242.0 14 775.0 15 1708.0 16 3098.0 17 5043.0 18 8974.0 19 19529.0 20 21155.0 21 18500.0 22 16954.0 23 17331.0 24 19664.0 25 24020.0 26 32088.0 27 43054.0 28 57115.0 29 74398.0 30 97530.0 31 127324.0 32 167030.0 33 220465.0 34 299559.0 35 424961.0 36 698048.0 37 1336863.0 38 1620058.0 39 362214.0 40 475.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.490051748431032 40.80590517258717 12.25796534283882 20.446077736142986 2 24.86827680050561 27.675591147697343 29.640898311211437 17.815233740585608 3 23.147293022452185 27.32002726013122 24.6338165880032 24.8988631294134 4 24.246552598600342 25.91913646091808 25.98540858566923 23.84890235481235 5 23.83864198798197 27.191211423233124 24.98074145528319 23.98940513350172 6 24.006893028817778 25.473340490545954 26.0333203864615 24.486446094174767 7 24.620228493342623 27.049576959068354 24.892025362344818 23.438169185244202 8 23.967667679623855 25.14425327648835 26.691267471937614 24.196811571950185 9 23.769652240960113 25.196087398205147 26.426028563678937 24.608231797155803 10-11 24.512721656887145 25.622066296261387 25.60077478371408 24.26443726313739 12-13 24.362002231105684 25.754047442211686 25.42120907460878 24.462741252073847 14-15 24.053008608766227 25.86214012316025 25.605933712832318 24.4789175552412 16-17 24.113822764727825 26.178076439940185 25.524518816188408 24.183581979143586 18-19 23.878339510349424 26.19162955881013 25.98822784838904 23.941803082451404 20-21 23.25536821550543 26.239222865912787 26.335493729627697 24.169915188954086 22-23 23.04766581526811 26.104664924346892 26.897785043418526 23.949884216966467 24-25 23.099542324291683 25.71554784067338 27.687211854134862 23.49769798090007 26-27 23.011833102099537 25.649463554204672 28.15265575229155 23.18604759140424 28-29 23.046408632049282 25.803444250619727 28.06517756821391 23.084969549117076 30-31 23.087853016274153 25.731980224329572 28.008258137536586 23.171908621859686 32-33 23.020877053079268 25.768680573043458 28.12647772741263 23.083964646464647 34-35 23.09099074348213 25.866127363292314 28.04528525467884 22.997596638546714 36-37 23.106475612509374 25.81657569751806 27.931116555289137 23.145832134683424 38-39 23.05649720626378 25.85202593421033 27.965981132126615 23.12549572739927 40-41 23.20896017661392 26.036589433925904 27.582739465697063 23.171710923763108 42-43 23.24497733652243 26.080495873636444 27.412900947863072 23.261625841978052 44-45 23.205265833091634 26.087910375629903 27.484603385860446 23.22222040541801 46-47 23.285146265836726 26.342400553457445 27.191552359114972 23.180900821590853 48-49 23.353886273768452 26.347822695825517 26.96518037627305 23.333110654132973 50-51 23.268674905173867 26.312790753290116 27.111858156770058 23.306676184765966 52-53 23.31944976254993 26.451748069895686 26.984770160346454 23.244032007207927 54-55 23.38168004812024 26.335506841299296 26.877288044062308 23.405525066518155 56-57 23.37839329037484 26.344292021678722 26.896202026045458 23.381112661900975 58-59 23.407650803027753 26.486624831611365 26.76893538161066 23.336788983750225 60-61 23.46481839790592 26.417400112202827 26.645076016715702 23.47270547317555 62-63 23.44256713273212 26.382422234595914 26.73280200285963 23.442208629812335 64-65 23.475960901160605 26.57035591108151 26.60401145390196 23.34967173385592 66-67 23.567122419503512 26.468176958923134 26.471884399213675 23.49281622235968 68-69 23.497778495952744 26.50699573536978 26.527360542604505 23.46786522607297 70-71 23.50045219526966 26.606587317319537 26.472410842158713 23.42054964525209 72-73 23.564424101731365 26.512623253690187 26.377432972560683 23.54551967201776 74-75 23.519355125851646 26.509788655557927 26.5091153668785 23.46174085171193 76-77 23.54291715177729 26.680732870804132 26.346956361685653 23.42939361573293 78-79 23.60390305647554 26.571102821647898 26.27656271299727 23.548431408879296 80-81 23.485987251845607 26.595965419076972 26.40811914226398 23.509928186813443 82-83 23.549179682092984 26.711968281175007 26.31466976418904 23.42418227254297 84-85 23.582855287141623 26.62318709547219 26.25280221662571 23.541155400760477 86-87 23.53964218704395 26.596168855481555 26.376485837685344 23.487703119789153 88-89 23.556529946310185 26.722158323127648 26.25230906766917 23.469002662892997 90-91 23.590529022370045 26.631637743657254 26.209356501586946 23.568476732385754 92-93 23.566959960176415 26.569625387762745 26.357269076501872 23.506145575558964 94-95 23.569931732503054 26.783612303154065 26.208391706470575 23.438064257872306 96-97 23.62451394359953 26.683064557733488 26.151395146912105 23.541026351754873 98-99 23.570375112829744 26.666712718136417 26.28181278521125 23.48109938382259 100-101 23.61479105768828 26.792376041681514 26.119129092734827 23.473703807895376 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 912.0 1 660.5 2 321.0 3 208.5 4 175.0 5 148.5 6 137.0 7 142.0 8 135.5 9 137.5 10 138.5 11 144.5 12 167.5 13 240.5 14 328.5 15 435.0 16 1923.5 17 2504.0 18 1870.0 19 2368.5 20 3271.0 21 4556.5 22 6084.0 23 8062.5 24 10692.5 25 14220.0 26 18662.0 27 23436.5 28 29263.0 29 36135.5 30 43803.0 31 52830.0 32 62265.5 33 72310.0 34 82202.5 35 93812.0 36 107873.5 37 122545.5 38 138301.0 39 154624.5 40 169601.0 41 181618.5 42 194064.5 43 207526.0 44 218414.5 45 227489.0 46 235836.5 47 241500.0 48 242766.0 49 241259.0 50 238520.0 51 235964.0 52 233941.0 53 228288.5 54 216770.0 55 201711.5 56 183123.0 57 162420.0 58 143819.5 59 124942.5 60 103332.0 61 83132.5 62 67281.0 63 54251.5 64 42999.0 65 33347.0 66 25251.5 67 18948.5 68 14433.5 69 11323.0 70 9044.5 71 7308.5 72 6097.0 73 4904.5 74 3615.5 75 2411.0 76 1410.5 77 894.5 78 587.0 79 297.5 80 104.0 81 38.0 82 24.0 83 13.0 84 7.5 85 6.5 86 5.5 87 2.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.04221577929296789 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.371973829014902E-5 24-25 0.0 26-27 4.371973829014902E-5 28-29 2.798063250569537E-4 30-31 4.371973829014902E-5 32-33 1.7487895316059606E-5 34-35 0.0 36-37 3.497579063211921E-5 38-39 4.371973829014902E-5 40-41 9.618342423832783E-5 42-43 1.7487895316059608E-4 44-45 3.410139586631623E-4 46-47 9.618342423832783E-5 48-49 0.0 50-51 2.1859869145074507E-4 52-53 0.001600142421419454 54-55 0.0011454571432019042 56-57 1.3990316252847685E-4 58-59 0.0012241526721241726 60-61 4.3719738290149015E-4 62-63 2.9729422037301327E-4 64-65 2.623184297408941E-4 66-67 1.7487895316059608E-4 68-69 7.519794985905631E-4 70-71 4.4594133055951996E-4 72-73 7.869552892226823E-5 74-75 6.995158126423843E-4 76-77 7.607234462485929E-4 78-79 7.869552892226823E-5 80-81 2.623184297408941E-5 82-83 0.002142267176217302 84-85 0.0 86-87 3.497579063211921E-5 88-89 4.1970948758543057E-4 90-91 2.448305344248345E-4 92-93 3.7598974929528155E-4 94-95 0.0 96-97 4.5468527821754983E-4 98-99 2.623184297408941E-5 100-101 0.008787667396319952 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5718241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.84510330690487 #Duplication Level Percentage of deduplicated Percentage of total 1 81.14950551382137 54.24447079375636 2 10.981017054280674 14.680544388165517 3 3.3865527360665393 6.791234024899475 4 1.4785530631772066 3.9533612901128405 5 0.825435471587832 2.758815968573619 6 0.5078480559582838 2.0368293458845366 7 0.3419282167487136 1.5999358880479462 8 0.24268949452324182 1.2978083466325296 9 0.1761224523431363 1.059563116938814 >10 0.8984397702639384 10.059694187938724 >50 0.010339029538921123 0.4256393441682282 >100 0.0013075844194902863 0.18920522882697596 >500 2.6156168974789542E-5 0.010403050312534369 >1k 1.8308974572804704E-4 0.2930632871994849 >5k 0.0 0.0 >10k+ 5.23113559203832E-5 0.5994317385424252 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21602 0.3777735146175196 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 12683 0.221798976293584 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.497579063211921E-5 0.0 0.0 0.0 0.0 2 3.497579063211921E-5 0.0 0.0 0.0 0.0 3 5.246368594817882E-5 0.0 0.0 0.0 0.0 4 6.995158126423842E-5 0.0 0.0 0.0 0.0 5 1.0492737189635764E-4 0.0 0.0 1.7487895316059606E-5 0.0 6 1.7487895316059605E-4 0.0 0.0 1.7487895316059606E-5 0.0 7 2.448305344248345E-4 0.0 0.0 1.7487895316059606E-5 0.0 8 3.8473369695331137E-4 0.0 0.0 1.7487895316059606E-5 0.0 9 4.89661068849669E-4 1.7487895316059606E-5 0.0 1.7487895316059606E-5 0.0 10-11 7.782113415646524E-4 2.623184297408941E-5 0.0 3.497579063211921E-5 0.0 12-13 0.001285360305730381 3.497579063211921E-5 0.0 3.497579063211921E-5 0.0 14-15 0.0018099971652121693 3.497579063211921E-5 0.0 3.497579063211921E-5 0.0 16-17 0.002264682443429719 3.497579063211921E-5 0.0 6.120763360620862E-5 0.0 18-19 0.0027106237739892387 4.371973829014902E-5 0.0 7.869552892226823E-5 0.0 20-21 0.0032002848428389083 1.2241526721241725E-4 0.0 8.743947658029803E-5 0.0 22-23 0.003689945911688577 2.448305344248345E-4 0.0 8.743947658029803E-5 0.0 24-25 0.004424437514963081 4.1096553992740076E-4 0.0 8.743947658029803E-5 0.0 26-27 0.005080233589315315 5.158929118237584E-4 0.0 8.743947658029803E-5 0.0 28-29 0.00574477361132558 5.77100545429967E-4 0.0 1.0492737189635764E-4 0.0 30-31 0.006522984952890233 6.557960743522352E-4 0.0 1.0492737189635764E-4 0.0 32-33 0.007642210253118048 7.344916032745035E-4 0.0 1.1367131955438744E-4 0.0 34-35 0.009233608726879472 7.344916032745035E-4 0.0 1.2241526721241725E-4 0.0 36-37 0.011209740897594208 7.344916032745035E-4 0.0 1.2241526721241725E-4 0.0 38-39 0.014348818106826908 7.344916032745035E-4 0.0 1.2241526721241725E-4 0.0 40-41 0.01823113086699214 7.519794985905631E-4 0.0 1.2241526721241725E-4 0.0 42-43 0.02353870709541623 7.519794985905631E-4 0.0 1.2241526721241725E-4 8.743947658029803E-6 44-45 0.030437681797601746 7.519794985905631E-4 0.0 1.4864711018650664E-4 3.497579063211921E-5 46-47 0.03904172629310307 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 48-49 0.0492021934717337 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 50-51 0.06224816337751417 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 52-53 0.0774276565118539 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 54-55 0.0947931365607011 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 56-57 0.11662677386280151 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 58-59 0.14184431890855947 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 60-61 0.170165965372918 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 62-63 0.20429359308220832 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 64-65 0.24330034358467928 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 66-67 0.28685394686932575 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 68-69 0.3364321300903547 7.519794985905631E-4 0.0 1.5739105784453645E-4 3.497579063211921E-5 70-71 0.39122170611556945 7.519794985905631E-4 0.0 1.7487895316059605E-4 3.497579063211921E-5 72-73 0.4533124784352391 7.519794985905631E-4 0.0 1.8362290081862587E-4 3.497579063211921E-5 74-75 0.5232203399611874 7.519794985905631E-4 0.0 1.9236684847665568E-4 3.497579063211921E-5 76-77 0.6019945644123779 7.519794985905631E-4 0.0 1.9236684847665568E-4 3.497579063211921E-5 78-79 0.6876065559321476 7.519794985905631E-4 0.0 1.9236684847665568E-4 3.497579063211921E-5 80-81 0.7813591627215433 7.519794985905631E-4 0.0 1.9236684847665568E-4 3.497579063211921E-5 82-83 0.8843716100807923 7.519794985905631E-4 0.0 1.9236684847665568E-4 3.497579063211921E-5 84-85 0.9980166977922057 7.519794985905631E-4 0.0 1.9236684847665568E-4 3.497579063211921E-5 86-87 1.1223294016464154 7.519794985905631E-4 0.0 2.0111079613468547E-4 3.497579063211921E-5 88-89 1.256636437673753 7.519794985905631E-4 0.0 2.0985474379271529E-4 3.497579063211921E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3735 0.0 76.719025 1 TCGACGG 4430 0.0 65.9702 2 CGACGGC 5450 0.0 53.623478 3 ATCTCGA 8365 0.0 51.63858 1 ACGGCGC 5815 0.0 50.23475 5 TATCTCG 4080 0.0 49.150585 2 CGGCGCG 5975 0.0 48.969048 6 ATATCTC 4215 0.0 48.591022 1 GACGGCG 6025 0.0 48.50589 4 TCTCGAG 8635 0.0 47.27235 2 TCGAGGG 9480 0.0 43.008602 4 CTCGAGG 9650 0.0 42.349426 3 GGGCGCG 9945 0.0 40.883476 8 CGAGGGC 10110 0.0 40.310204 5 AGGGCGC 10280 0.0 39.597393 7 GAGGGCG 10710 0.0 38.00758 6 GGCGCGC 15765 0.0 27.357145 9 CGCGCCG 15565 0.0 24.931696 9 GCGCGCC 15810 0.0 24.635471 8 TCCATAT 8125 0.0 20.197775 18-19 >>END_MODULE