FastQCFastQC Report
Tue 31 May 2016
SRR2048239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523912
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT120500.218142504804566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG168700.091.5340041
TCGACGG183900.083.988892
TATCTCG70700.082.949912
CGACGGC193550.079.7031563
ACGGCGC194350.079.36075
CGGCGCG195950.078.639266
GACGGCG196550.078.557754
ATCTCGA77600.075.451723
ATATCTC82900.071.2276461
TCTCGAG69100.068.913134
TCGAGGG70650.066.831026
AGGGCGC71700.066.236039
CTCGAGG71850.066.0002065
CGAGGGC75450.062.5776447
CGCGCCG258550.060.1561749
GGCGCGC259050.059.995937
GCGCGCC260000.059.844378
GAGGGCG80500.058.8826568
TCTCGAC19900.055.1659624
CTCGACG20500.053.3108335
Produced by FastQC (version 0.11.5)