##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048239_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5523912 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04932989519022 31.0 31.0 34.0 28.0 34.0 2 31.43336190004475 31.0 31.0 34.0 28.0 34.0 3 31.498429373965408 31.0 31.0 34.0 28.0 34.0 4 35.09381702677378 37.0 35.0 37.0 32.0 37.0 5 35.10217107006773 37.0 35.0 37.0 32.0 37.0 6 35.143979122042495 37.0 35.0 37.0 32.0 37.0 7 34.92706491341644 37.0 35.0 37.0 32.0 37.0 8 35.04850801388581 37.0 35.0 37.0 32.0 37.0 9 36.63040377906093 39.0 37.0 39.0 32.0 39.0 10-11 36.505707549287536 39.0 37.0 39.0 32.0 39.0 12-13 36.36741859754464 39.0 36.0 39.0 32.0 39.0 14-15 37.58940566033637 40.0 37.0 41.0 32.0 41.0 16-17 37.67874470121899 40.0 37.0 41.0 32.0 41.0 18-19 37.605184514163156 40.0 37.0 41.0 32.0 41.0 20-21 37.59488456731389 40.0 37.0 41.0 32.0 41.0 22-23 37.74053750313183 40.0 37.0 41.0 32.0 41.0 24-25 37.585930858420625 40.0 37.0 41.0 32.0 41.0 26-27 37.65525039138929 40.0 37.0 41.0 32.0 41.0 28-29 37.54191594652485 40.0 37.0 41.0 31.0 41.0 30-31 37.323354897760865 40.0 36.5 41.0 31.0 41.0 32-33 37.12357953928303 39.5 35.5 40.5 30.5 41.0 34-35 37.09556261939002 39.0 35.5 41.0 30.5 41.0 36-37 36.80404276896518 39.0 35.0 40.5 30.0 41.0 38-39 36.673022940988204 39.0 35.0 40.0 30.0 41.0 40-41 36.506216789840245 39.0 35.0 40.5 29.5 41.0 42-43 36.50127364447515 39.0 35.0 40.5 29.0 41.0 44-45 36.32703788546958 39.0 35.0 40.0 29.0 41.0 46-47 36.22673225062239 39.0 35.0 40.0 29.0 41.0 48-49 36.11141705371121 38.5 34.0 40.0 28.0 41.0 50-51 36.061675584259845 38.0 34.0 40.0 28.0 41.0 52-53 35.89470378963314 38.0 34.0 40.0 28.0 41.0 54-55 35.841563551338254 38.0 34.0 40.0 28.0 41.0 56-57 35.776301287927836 38.0 34.0 40.0 28.0 41.0 58-59 35.65936414265832 38.0 34.0 40.0 28.0 41.0 60-61 35.434501219425655 38.0 34.0 40.0 28.0 41.0 62-63 35.14281961769123 37.0 34.0 40.0 28.0 41.0 64-65 34.81613935920775 37.0 33.0 40.0 27.0 41.0 66-67 34.48875706202416 36.0 33.0 39.0 26.5 41.0 68-69 34.14942299949746 36.0 33.0 39.0 26.0 41.0 70-71 33.76709368288272 35.0 32.5 39.0 26.0 40.0 72-73 33.47668400582775 35.0 32.0 38.0 26.0 40.0 74-75 33.425969132020924 35.0 33.0 37.0 26.5 39.5 76-77 33.132136699498474 35.0 33.0 37.0 26.0 39.0 78-79 32.82266562175502 35.0 33.0 36.0 26.0 39.0 80-81 32.53080307941184 35.0 32.0 36.0 26.0 37.5 82-83 32.24868064516596 35.0 32.0 35.5 26.0 37.0 84-85 32.022659575315465 35.0 32.0 35.0 26.0 37.0 86-87 31.818609981476897 35.0 32.0 35.0 25.0 36.0 88-89 31.636154323240486 35.0 32.0 35.0 25.0 36.0 90-91 31.45877568288561 35.0 32.0 35.0 25.0 36.0 92-93 31.30401045490949 35.0 32.0 35.0 25.0 35.5 94-95 31.153434739727935 35.0 32.0 35.0 24.5 35.0 96-97 30.98466250005431 35.0 32.0 35.0 24.0 35.0 98-99 30.805321029734 35.0 32.0 35.0 23.5 35.0 100-101 30.207726517004616 34.5 31.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5318029197969274 1101 2 0.5666313408836743 1101 3 0.528723494509066 1101 4 0.48096937638713655 1101 5 0.47616515600420684 1101 6 0.532537725142987 1101 7 0.6024448587479299 1101 8 0.6294736509028453 1101 9 0.7411699358597872 1101 10-11 0.7303482251261144 1101 12-13 0.6941246309086466 1101 14-15 0.8438066896199885 1101 16-17 0.7753069817136335 1101 18-19 0.7627142510996734 1101 20-21 0.8578758017633135 1101 22-23 0.816124234214783 1101 24-25 0.7856268024325104 1101 26-27 0.7616849805215011 1101 28-29 0.8858395250741538 1101 30-31 0.837119149335031 1101 32-33 0.8485433478577491 1101 34-35 0.9449235844679507 1101 36-37 0.9074370171308601 1101 38-39 0.8806210048360228 1101 40-41 0.7611327759919675 1101 42-43 0.8004127981451461 1101 44-45 0.7941094956434398 1101 46-47 0.8021779961458293 1101 48-49 0.7847134288913651 1101 50-51 0.6638328470750139 1101 52-53 0.6847318970740659 1101 54-55 0.6117174705968864 1101 56-57 0.5323265334992158 1101 58-59 0.5476683053642901 1101 60-61 0.5112588567132264 1101 62-63 0.4989253351618217 1101 64-65 0.5116226089432985 1101 66-67 0.5402166058573954 1101 68-69 0.46309344843318456 1101 70-71 0.4660164262190136 1101 72-73 0.5275703597592525 1101 74-75 0.5307325105432312 1101 76-77 0.5239891982258769 1101 78-79 0.6277659643430624 1101 80-81 0.5509207301487677 1101 82-83 0.5602662740162927 1101 84-85 0.49961698074460514 1101 86-87 0.5202084647918532 1101 88-89 0.5049110974473301 1101 90-91 0.4637161011225146 1101 92-93 0.4874705454469428 1101 94-95 0.43591289971505276 1101 96-97 0.5187365116150389 1101 98-99 0.4907649381080681 1101 100-101 0.588848025631215 1102 1 0.35312635267245085 1102 2 0.40133147436258554 1102 3 0.4207900314884583 1102 4 0.370729960676222 1102 5 0.354328095806153 1102 6 0.4034142555321907 1102 7 0.4648110308623714 1102 8 0.5072018246577201 1102 9 0.5618547841782657 1102 10-11 0.555969262786256 1102 12-13 0.5712024607840931 1102 14-15 0.7217020664683389 1102 16-17 0.6651399052027855 1102 18-19 0.6667318188403115 1102 20-21 0.7345152625177676 1102 22-23 0.7122299778420285 1102 24-25 0.6316308531353769 1102 26-27 0.6251400702734102 1102 28-29 0.8086320093864501 1102 30-31 0.7343021974467021 1102 32-33 0.695636564685941 1102 34-35 0.7829021863308583 1102 36-37 0.8469977470221437 1102 38-39 0.8262188348997341 1102 40-41 0.725579710869404 1102 42-43 0.6790246020257911 1102 44-45 0.656564100200896 1102 46-47 0.6386780148701234 1102 48-49 0.6405402630248176 1102 50-51 0.5709605546023582 1102 52-53 0.6405910770179517 1102 54-55 0.5651535285176976 1102 56-57 0.48786133447199376 1102 58-59 0.5169699109495696 1102 60-61 0.47177860180677555 1102 62-63 0.4254024558763163 1102 64-65 0.3864303780067502 1102 66-67 0.46719973554908023 1102 68-69 0.43425020588649943 1102 70-71 0.44825965209655294 1102 72-73 0.4045978708437872 1102 74-75 0.39519842696951457 1102 76-77 0.42453927140847014 1102 78-79 0.36015929150278936 1102 80-81 0.3513673607601362 1102 82-83 0.34533552597400075 1102 84-85 0.3589928280699297 1102 86-87 0.40896132850476974 1102 88-89 0.3924976249385743 1102 90-91 0.4083160770396539 1102 92-93 0.44706733355722506 1102 94-95 0.5144732271437 1102 96-97 0.479420784903386 1102 98-99 0.45184639000098414 1102 100-101 0.4249841411982942 1103 1 0.3743275137224238 1103 2 0.38703521546589315 1103 3 0.40666830408379084 1103 4 0.27252716754902195 1103 5 0.2830475644824233 1103 6 0.3337639833981143 1103 7 0.3290525408922207 1103 8 0.41497491709603906 1103 9 0.53653680961623 1103 10-11 0.5142516911010873 1103 12-13 0.42659325030015793 1103 14-15 0.6348414828474986 1103 16-17 0.5590485223835415 1103 18-19 0.556749188879273 1103 20-21 0.6249072779097844 1103 22-23 0.55719017323419 1103 24-25 0.5164978559829407 1103 26-27 0.5874533449516974 1103 28-29 0.6545683604523944 1103 30-31 0.6431111810396999 1103 32-33 0.75904331032811 1103 34-35 0.7739936782322872 1103 36-37 0.8218963785082423 1103 38-39 0.7398980782226019 1103 40-41 0.6926671490691589 1103 42-43 0.7343680966241379 1103 44-45 0.7254346944494543 1103 46-47 0.748655969880474 1103 48-49 0.7255530723896797 1103 50-51 0.6976358751221667 1103 52-53 0.7384042909218778 1103 54-55 0.6857498548375673 1103 56-57 0.5739395131527658 1103 58-59 0.630412004413266 1103 60-61 0.5302631438226371 1103 62-63 0.5723157677464386 1103 64-65 0.529595028730796 1103 66-67 0.6128973005231018 1103 68-69 0.5067585610622416 1103 70-71 0.5314552042445939 1103 72-73 0.5364412194797907 1103 74-75 0.4493439654563076 1103 76-77 0.44660412959644447 1103 78-79 0.47316804301046034 1103 80-81 0.4804562544278639 1103 82-83 0.5477502418876412 1103 84-85 0.5172293899241964 1103 86-87 0.4987358348559461 1103 88-89 0.5569453782092246 1103 90-91 0.6003752577597439 1103 92-93 0.6296744133025349 1103 94-95 0.5993830577338777 1103 96-97 0.5771190729364477 1103 98-99 0.5637691244939127 1103 100-101 0.6374024724071248 1104 1 0.2817910454324988 1104 2 0.28105895930053393 1104 3 0.30427115538596894 1104 4 0.2695737558082314 1104 5 0.25870405680361586 1104 6 0.27268950018086 1104 7 0.32149257316205393 1104 8 0.3675203340073665 1104 9 0.45437552056450414 1104 10-11 0.37246890440339797 1104 12-13 0.34830337272532574 1104 14-15 0.4778199531158833 1104 16-17 0.47721231797022057 1104 18-19 0.4445402926613937 1104 20-21 0.5242170314182104 1104 22-23 0.44278454139562484 1104 24-25 0.38052540558252446 1104 26-27 0.401175583461189 1104 28-29 0.578635881243116 1104 30-31 0.47674957390572814 1104 32-33 0.47298496657859346 1104 34-35 0.5225544501502242 1104 36-37 0.5862186069895472 1104 38-39 0.5749359118864916 1104 40-41 0.5408104408804562 1104 42-43 0.6010391023005752 1104 44-45 0.5497114925177726 1104 46-47 0.5317873296280737 1104 48-49 0.5874125241173545 1104 50-51 0.45032955740455094 1104 52-53 0.44617509501699004 1104 54-55 0.4531616487237784 1104 56-57 0.3600926244004228 1104 58-59 0.3568376370261035 1104 60-61 0.37593757744471645 1104 62-63 0.31345515589858763 1104 64-65 0.30670005391524313 1104 66-67 0.41876600239996264 1104 68-69 0.4061874987697536 1104 70-71 0.43564960546948583 1104 72-73 0.3603065506580805 1104 74-75 0.3631406464790814 1104 76-77 0.4021430297784079 1104 78-79 0.3582508045410435 1104 80-81 0.3563417435682936 1104 82-83 0.3649473478803955 1104 84-85 0.3923636107179078 1104 86-87 0.38081106427487654 1104 88-89 0.4086466580366128 1104 90-91 0.4093184102793046 1104 92-93 0.43711854666854677 1104 94-95 0.46850421112993956 1104 96-97 0.52395657436265 1104 98-99 0.5288455533157581 1104 100-101 0.5034697616513419 1105 1 0.18981327581955298 1105 2 0.1850084342827394 1105 3 0.2757336656482714 1105 4 0.18994385879204856 1105 5 0.24014386661932008 1105 6 0.30166976537025647 1105 7 0.2337881290023205 1105 8 0.21763330641167755 1105 9 0.3089217960852295 1105 10-11 0.33484095234512523 1105 12-13 0.33126954105127737 1105 14-15 0.31271853382726533 1105 16-17 0.34948662587318324 1105 18-19 0.35566297332076857 1105 20-21 0.34941113262013346 1105 22-23 0.3659462156647777 1105 24-25 0.20767960862780654 1105 26-27 0.35796460703549116 1105 28-29 0.4075481906913083 1105 30-31 0.4337265823592986 1105 32-33 0.30964061767620876 1105 34-35 0.4132817100812076 1105 36-37 0.5035134926174152 1105 38-39 0.36734892253719664 1105 40-41 0.36054750151534876 1105 42-43 0.3947361061361647 1105 44-45 0.3355256957335371 1105 46-47 0.36649467834749316 1105 48-49 0.39540046721942446 1105 50-51 0.2833040048248918 1105 52-53 0.378867976743841 1105 54-55 0.3753498705936096 1105 56-57 0.2682779057350686 1105 58-59 0.29323486940696597 1105 60-61 0.1379812957077533 1105 62-63 0.1636322361808027 1105 64-65 0.10203127324610506 1105 66-67 0.22311901491883646 1105 68-69 0.1842135056809724 1105 70-71 0.23419561237680853 1105 72-73 0.2876623333465034 1105 74-75 0.2628968543710002 1105 76-77 0.2892777732319658 1105 78-79 0.2108389250953948 1105 80-81 0.2410371614083573 1105 82-83 0.3102933099372365 1105 84-85 0.25972156751167574 1105 86-87 0.2362632911885605 1105 88-89 0.2600092264813334 1105 90-91 0.29046832872115047 1105 92-93 0.30007305709510845 1105 94-95 0.25073517334649154 1105 96-97 0.2784512006275577 1105 98-99 0.2432292918644201 1105 100-101 0.2655948732786655 1106 1 0.20808367629859248 1106 2 0.23069157404504637 1106 3 0.2973110045329008 1106 4 0.2166053743029579 1106 5 0.20368215255190592 1106 6 0.2639736016118803 1106 7 0.27601212232978867 1106 8 0.28527334247628033 1106 9 0.29842510953172763 1106 10-11 0.3367279129492786 1106 12-13 0.3152048350398786 1106 14-15 0.3681286560977952 1106 16-17 0.3528754510053531 1106 18-19 0.35279459226507726 1106 20-21 0.4503868151028456 1106 22-23 0.37603493290632883 1106 24-25 0.26703343876673813 1106 26-27 0.32323721167011144 1106 28-29 0.4242660313081643 1106 30-31 0.4167655492146949 1106 32-33 0.4427945703354226 1106 34-35 0.5252173943384548 1106 36-37 0.5286997861067633 1106 38-39 0.5850927043863479 1106 40-41 0.5275316275439152 1106 42-43 0.48769559398827766 1106 44-45 0.4338976867336015 1106 46-47 0.36843284950985833 1106 48-49 0.5097082497518173 1106 50-51 0.4290525617704688 1106 52-53 0.367892219960396 1106 54-55 0.3997327128628356 1106 56-57 0.38694720893875 1106 58-59 0.3642065339556595 1106 60-61 0.39945674524140884 1106 62-63 0.38673828005440924 1106 64-65 0.33071676641625913 1106 66-67 0.35770441589370705 1106 68-69 0.331314674884311 1106 70-71 0.3060427510434778 1106 72-73 0.32029916648016865 1106 74-75 0.294616120938187 1106 76-77 0.23798176918219127 1106 78-79 0.3652642345708159 1106 80-81 0.3904501341152269 1106 82-83 0.42858827575632574 1106 84-85 0.4021737617030894 1106 86-87 0.36255861527817146 1106 88-89 0.35718261653703465 1106 90-91 0.21571879065340482 1106 92-93 0.22954535296951306 1106 94-95 0.25284169396504197 1106 96-97 0.31340785713373265 1106 98-99 0.21431845054450704 1106 100-101 0.29295826630124466 1107 1 0.13732673170293097 1107 2 0.13905050174217237 1107 3 0.16428804463150115 1107 4 0.13346684630909067 1107 5 0.1499679712765456 1107 6 0.09652140752130123 1107 7 0.1740988996195867 1107 8 -0.15984776740859274 1107 9 -0.09060693843490242 1107 10-11 -0.02179496977017692 1107 12-13 0.0011558334572185913 1107 14-15 -0.10753534570800838 1107 16-17 -0.035704635278875685 1107 18-19 0.04463685771877124 1107 20-21 0.06430166725739639 1107 22-23 0.05364979709091244 1107 24-25 -0.018520970078917287 1107 26-27 0.08997488535911202 1107 28-29 -0.08898037245555201 1107 30-31 0.015055883082624177 1107 32-33 0.04752742651335495 1107 34-35 0.12968999054692887 1107 36-37 0.3030442193380267 1107 38-39 0.18942084047923657 1107 40-41 -0.13264845968891592 1107 42-43 -0.030189339062857812 1107 44-45 -0.08316977534538239 1107 46-47 0.001557946122019871 1107 48-49 0.09182714884850895 1107 50-51 0.011155385788548244 1107 52-53 0.15911564743772288 1107 54-55 0.10860903710975833 1107 56-57 -0.11269407291954536 1107 58-59 0.06666712319878343 1107 60-61 -0.132186191393302 1107 62-63 -0.00852060389910747 1107 64-65 -0.23606518717497949 1107 66-67 0.03778512707135917 1107 68-69 0.1639136569451196 1107 70-71 0.19028738997106842 1107 72-73 0.2566583075055746 1107 74-75 0.18455376310763683 1107 76-77 0.22785937436899673 1107 78-79 0.2765842375632346 1107 80-81 0.20136537120929177 1107 82-83 0.15888005419846252 1107 84-85 0.19756753348731593 1107 86-87 0.18921984327728225 1107 88-89 0.20422224730554817 1107 90-91 0.1648396711483855 1107 92-93 0.13073987548820298 1107 94-95 0.11443026799857847 1107 96-97 0.13471224371951251 1107 98-99 0.10122416849712934 1107 100-101 0.13687321439423528 1108 1 -0.1441222648225704 1108 2 -0.16452333755531967 1108 3 0.002017627579267156 1108 4 -0.16541495546250928 1108 5 -0.05913290555166384 1108 6 0.014716990638838467 1108 7 0.025596416970337543 1108 8 0.15574479265999486 1108 9 0.12781438982349158 1108 10-11 0.13037453670367682 1108 12-13 0.22512827910362887 1108 14-15 0.2414233704893789 1108 16-17 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-0.7832041235763256 2215 18-19 -0.7276821240691405 2215 20-21 -3.587238395737792 2215 22-23 -1.3934798439757543 2215 24-25 -0.8770339220545438 2215 26-27 -0.7567813704807378 2215 28-29 -0.6712479686909276 2215 30-31 -1.1146028870355877 2215 32-33 -1.0226471686057153 2215 34-35 -1.149471692476439 2215 36-37 -1.2000517831737625 2215 38-39 -1.0508984896652223 2215 40-41 -1.3092630253895337 2215 42-43 -1.6719988957694696 2215 44-45 -1.087045073353508 2215 46-47 -0.9618580970974691 2215 48-49 -0.9957206370018952 2215 50-51 -0.8704627281475368 2215 52-53 -0.7824451595853716 2215 54-55 -0.8451969479723473 2215 56-57 -0.6296062193853658 2215 58-59 -0.5434481799032014 2215 60-61 -0.5832413104612826 2215 62-63 -0.5610125818366711 2215 64-65 -0.5746458226583471 2215 66-67 -0.6851970322670482 2215 68-69 -0.5240234122102265 2215 70-71 -0.3409870802699402 2215 72-73 -0.3654134214432929 2215 74-75 -0.33693362120150283 2215 76-77 -0.43633192111527563 2215 78-79 -0.47413977169279065 2215 80-81 -0.38417000088279707 2215 82-83 -0.46139699729762285 2215 84-85 -0.596204651741786 2215 86-87 -0.5955793521398469 2215 88-89 -0.6349242048200558 2215 90-91 -0.617289347737561 2215 92-93 -0.5780402948874581 2215 94-95 -0.5134805960042996 2215 96-97 -0.5772827673018952 2215 98-99 -0.6083600067606945 2215 100-101 -0.5438322905766881 2216 1 -0.5256077658337652 2216 2 -0.5511445856171342 2216 3 -0.544036083576092 2216 4 -0.427502069624083 2216 5 -0.46368053932125974 2216 6 -0.5662122383935042 2216 7 -0.6329650698185887 2216 8 -1.6522346787219888 2216 9 -1.0620818231441476 2216 10-11 -0.7988188740591937 2216 12-13 -0.7835170071964939 2216 14-15 -1.6056910074495292 2216 16-17 -0.9348721927227146 2216 18-19 -0.9516361263697064 2216 20-21 -0.9225171525202995 2216 22-23 -0.8332481216072125 2216 24-25 -1.020245237888716 2216 26-27 -1.3146183916208614 2216 28-29 -2.0394442226878127 2216 30-31 -1.168249153896106 2216 32-33 -1.0367005064599724 2216 34-35 -2.328115365041114 2216 36-37 -2.275854482722721 2216 38-39 -2.3699068878043974 2216 40-41 -1.4885378477409645 2216 42-43 -1.4621655202355868 2216 44-45 -1.2413145192387702 2216 46-47 -1.5962477354541207 2216 48-49 -1.5367263279577656 2216 50-51 -1.0361877230762886 2216 52-53 -0.910283722788499 2216 54-55 -0.8080029999348284 2216 56-57 -0.8443612895075887 2216 58-59 -0.8794962997027156 2216 60-61 -0.9104205467727553 2216 62-63 -0.84542908692449 2216 64-65 -0.793828613094071 2216 66-67 -0.8061327609310212 2216 68-69 -0.9549834823551038 2216 70-71 -1.1102239432916292 2216 72-73 -0.8967225773230467 2216 74-75 -0.7525325706561645 2216 76-77 -0.9054362519059111 2216 78-79 -0.8977477566491601 2216 80-81 -0.8659332066896361 2216 82-83 -1.209918669183672 2216 84-85 -0.8270550994921493 2216 86-87 -1.0207103820531849 2216 88-89 -0.8082754867467479 2216 90-91 -1.015069472883951 2216 92-93 -1.317653358342401 2216 94-95 -0.9704156106637996 2216 96-97 -0.8582517821718696 2216 98-99 -0.8211954539601898 2216 100-101 -0.8490284609863714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 85.0 11 1090.0 12 4245.0 13 8659.0 14 12289.0 15 14549.0 16 16312.0 17 18549.0 18 23161.0 19 31637.0 20 31418.0 21 32979.0 22 40032.0 23 47601.0 24 55275.0 25 61419.0 26 70752.0 27 82658.0 28 97622.0 29 113830.0 30 132878.0 31 158260.0 32 192237.0 33 243471.0 34 319336.0 35 448238.0 36 715923.0 37 1182185.0 38 1150429.0 39 216642.0 40 150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.43926953595599 40.39199016790338 11.968888944453836 21.199851351686796 2 23.859621670208288 27.471027237448514 31.684028862556573 16.98532222978662 3 22.119991450917034 27.782273693819665 23.752811960022168 26.34492289524113 4 24.79128262264699 26.484007677952043 25.572934657527075 23.151775041873893 5 23.011701466803586 29.261454705489236 24.662675019423965 23.06416880828321 6 22.415787279978304 25.384566080111128 26.396773624664082 25.802873015246487 7 25.67478134688832 26.254035777937563 24.456375211872118 23.614807663302003 8 25.488307435656544 25.03862336600937 26.248825185204765 23.22424401312932 9 21.809544085582345 25.746158954160904 26.377758177587857 26.066538782668896 10-11 24.91868904620251 25.52909597933038 25.295029414235277 24.25718556023184 12-13 24.457283844948172 25.833435616474066 24.95716780336727 24.752112735210492 14-15 24.29889326949849 25.704664039749176 25.227586326753247 24.768856363999088 16-17 24.415807930287073 27.193160787710013 25.09106319779591 23.299968084207006 18-19 22.8675140387373 25.5895056738784 26.654447472339353 24.888532815044943 20-21 22.81269365735337 27.48343519608285 25.42300695070922 24.280864195854555 22-23 22.346205862513592 27.196441887398798 27.328733149793127 23.128619100294483 24-25 22.299063130991005 25.77823374530051 28.740529844360946 23.182173279347534 26-27 22.237387086536163 25.628461769786437 29.560945343664468 22.57320580001293 28-29 22.277819583178825 25.583239663483827 29.669312236372487 22.469628516964864 30-31 22.387559567381075 25.511236055233887 29.539990059544074 22.561214317840964 32-33 22.30999522683747 25.545770356117032 29.636940023533604 22.507294393511902 34-35 22.362060055955048 25.59426100968365 29.64393715778811 22.399741776573194 36-37 22.37914947538862 25.522509097298023 29.491629381495976 22.60671204581738 38-39 22.473115213401318 25.622823157194357 29.258559702353875 22.645501927050447 40-41 22.930347380105378 26.117191193521077 27.823392853441355 23.12906857293219 42-43 22.933184208911296 26.238798696332 27.767330056723644 23.060687038033066 44-45 23.027370201678888 26.496054061520542 27.461506891746794 23.015068845053776 46-47 22.938770566946033 26.85401215660206 27.29456044918891 22.912656827262996 48-49 22.972306582726155 26.876912593828433 27.10464974822191 23.0461310752235 50-51 22.89645460072031 26.88712429771086 27.17897461168393 23.0374464898849 52-53 22.96773748550241 26.950456876004917 27.09790163898219 22.98390399951048 54-55 23.00828722255347 26.755256330194126 27.078279960269704 23.158176486982697 56-57 23.003380575215534 26.699049514184875 27.201926822874807 23.09564308772479 58-59 23.019367081663923 26.82250381133783 27.105052437237614 23.053076669760635 60-61 23.05711966446967 26.763677625566807 26.983766214957804 23.195436495005715 62-63 23.091416010971937 26.755621740534608 27.024561578823125 23.128400669670334 64-65 23.106807101645792 26.977220350520202 26.87046393870875 23.045508609125257 66-67 23.20721787992473 26.91879378195832 26.696649745329086 23.17733859278786 68-69 23.16226254147423 26.911362816786365 26.773073140918974 23.153301500820433 70-71 23.21077384000305 27.03425855264474 26.64503807707706 23.109929530275153 72-73 23.25737654910473 26.97224080358379 26.548525414324132 23.221857232987343 74-75 23.235580744913754 26.953994199149733 26.697661139607646 23.112763916328863 76-77 23.29728505542203 27.129287540669495 26.504798511748078 23.06862889216039 78-79 23.347262792106562 27.02343967025498 26.44202121886792 23.187276318770536 80-81 23.3271483394699 26.972158113896395 26.53251032091999 23.168183225713715 82-83 23.418750083727712 27.141664394703845 26.399022205132717 23.040563316435723 84-85 23.47570888167661 27.05996221518373 26.313661405178067 23.15066749796159 86-87 23.456782077629043 27.037921675797875 26.413056543985498 23.092239702587587 88-89 23.518640412808896 27.13380481079351 26.31358899272834 23.033965783669256 90-91 23.587776199186376 27.042537969468018 26.25425604173274 23.115429789612868 92-93 23.593885999632143 27.011907503233218 26.348473690384637 23.04573280675 94-95 23.66457865367877 27.165611979336386 26.224458318669814 22.945351048315036 96-97 23.765603072605067 27.09536285154434 26.148434298011992 22.990599777838604 98-99 23.7488305389369 27.08340574578306 26.247747972813467 22.92001574246657 100-101 23.82754287179086 27.214770981145247 26.084304022221932 22.87338212484196 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2492.0 1 1823.5 2 936.0 3 585.5 4 393.0 5 269.5 6 197.0 7 173.5 8 155.0 9 142.0 10 139.5 11 144.0 12 149.5 13 180.0 14 228.5 15 365.0 16 1504.0 17 1958.0 18 1573.5 19 2145.5 20 3174.0 21 4570.0 22 6318.0 23 8668.0 24 11657.5 25 15540.0 26 20479.5 27 26497.5 28 33693.0 29 41826.5 30 51050.0 31 61168.0 32 71377.5 33 81943.5 34 92027.5 35 103248.0 36 115937.5 37 129255.0 38 143632.5 39 157742.5 40 170820.0 41 180968.0 42 191505.0 43 202228.0 44 210566.0 45 218188.5 46 225036.0 47 228905.5 48 228243.5 49 225329.5 50 221931.5 51 218408.0 52 215138.0 53 208780.5 54 197685.5 55 183683.5 56 165346.5 57 146112.5 58 129696.5 59 112686.0 60 92922.0 61 75089.5 62 61335.0 63 49280.0 64 39134.0 65 30622.5 66 23293.0 67 17435.0 68 13214.5 69 10492.5 70 8451.5 71 6921.5 72 5861.5 73 4680.5 74 3517.5 75 2429.5 76 1517.5 77 968.0 78 599.0 79 348.0 80 164.0 81 66.5 82 40.5 83 34.5 84 24.0 85 11.0 86 4.5 87 6.0 88 5.5 89 1.5 90 1.5 91 4.0 92 4.0 93 1.5 94 2.5 95 4.5 96 4.0 97 2.0 98 1.5 99 3.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08770957973262428 2 0.04404487254684723 3 0.051630076655819285 4 0.05736876329673608 5 0.04312161381281961 6 0.016908307011407858 7 0.02032979526103964 8 0.012690281814771848 9 0.0028240855393786143 10-11 0.0010137742961871948 12-13 5.430933729574258E-4 14-15 0.0013396303199616503 16-17 1.8103112431914194E-5 18-19 0.0016111770064403633 20-21 0.005485243066870001 22-23 0.01577686248441322 24-25 0.019940578343753484 26-27 0.009866196275393237 28-29 0.01752381283409294 30-31 3.71113804854241E-4 32-33 0.005802047534428499 34-35 2.4439201783084164E-4 36-37 0.002833137095594571 38-39 0.010400238092134705 40-41 0.001674537899952063 42-43 0.002045651704806304 44-45 0.0023715077285807597 46-47 0.0 48-49 0.0 50-51 0.003439591362063697 52-53 0.0026883121961392576 54-55 0.0030322713323456276 56-57 0.0 58-59 0.004724912344729605 60-61 0.0 62-63 0.0 64-65 0.002208579716693532 66-67 3.349075799904126E-4 68-69 0.0 70-71 9.142071778116669E-4 72-73 0.0028421886518105286 74-75 0.004326643871227493 76-77 0.0020999610421020467 78-79 1.3577334323935645E-4 80-81 0.0010590320772669803 82-83 9.775680713233665E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5523912.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.70531421333538 #Duplication Level Percentage of deduplicated Percentage of total 1 82.93600575813636 57.810803409698885 2 9.984793128519717 13.91986284757238 3 3.063504544258722 6.406276405546052 4 1.337327037204576 3.7287520533733534 5 0.7372710987101673 2.569585679800161 6 0.46800957506955027 1.9573652691043546 7 0.30802716052072787 1.5029791007237183 8 0.2260942129917972 1.2607974526728 9 0.1617950868674238 1.0150179631440903 >10 0.7666727932475447 8.820905480726267 >50 0.009019917493514433 0.39206920739772 >100 0.0011668910883138434 0.15247926743893386 >500 1.3033229055882926E-4 0.0612318307004831 >1k 1.5639737229660686E-4 0.18293099540761132 >5k 0.0 0.0 >10k+ 2.606622869231196E-5 0.21894303669318876 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 12050 0.218142504804566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.8103112431914194E-5 0.0 0.0 0.0 0.0 2 1.8103112431914194E-5 0.0 0.0 0.0 0.0 3 3.620622486382839E-5 0.0 0.0 0.0 0.0 4 7.241244972765678E-5 0.0 0.0 0.0 0.0 5 1.0861867459148517E-4 0.0 0.0 0.0 0.0 6 1.6292801188722776E-4 0.0 0.0 0.0 0.0 7 2.3534046161488453E-4 0.0 0.0 0.0 0.0 8 3.077529113425413E-4 0.0 0.0 0.0 0.0 9 5.430933729574258E-4 0.0 0.0 0.0 0.0 10-11 0.0010047227399712377 0.0 1.8103112431914194E-5 9.051556215957097E-6 0.0 12-13 0.0015478161129286636 0.0 3.620622486382839E-5 1.8103112431914194E-5 0.0 14-15 0.0019822908112946042 0.0 4.525778107978549E-5 1.8103112431914194E-5 0.0 16-17 0.002416765509660545 0.0 6.336089351169968E-5 1.8103112431914194E-5 0.0 18-19 0.0027607246458669146 0.0 8.146400594361388E-5 1.8103112431914194E-5 0.0 20-21 0.0032495086815285982 0.0 1.8103112431914196E-4 1.8103112431914194E-5 0.0 22-23 0.00414561274690835 0.0 6.245573789010396E-4 1.8103112431914194E-5 0.0 24-25 0.00511412926201576 0.0 9.956711837552806E-4 2.715466864787129E-5 0.0 26-27 0.006046439552259341 1.8103112431914194E-5 0.0010771351896988945 5.430933729574258E-5 0.0 28-29 0.007069265404662493 3.620622486382839E-5 0.001131444526994637 7.241244972765678E-5 0.0 30-31 0.0084179472808401 3.620622486382839E-5 0.0012038569767222939 7.241244972765678E-5 0.0 32-33 0.00988429938782515 3.620622486382839E-5 0.0012853209826659078 7.241244972765678E-5 0.0 34-35 0.011866590199119755 3.620622486382839E-5 0.001303424095097822 7.241244972765678E-5 0.0 36-37 0.014953170868761124 3.620622486382839E-5 0.0013305787637456932 8.146400594361388E-5 0.0 38-39 0.0194517943080918 3.620622486382839E-5 0.0013396303199616503 9.051556215957097E-5 0.0 40-41 0.024737903138210747 3.620622486382839E-5 0.0013486818761776075 9.051556215957097E-5 0.0 42-43 0.032033457448272164 5.430933729574258E-5 0.0013577334323935646 9.051556215957097E-5 0.0 44-45 0.04051476562262396 5.430933729574258E-5 0.0013577334323935646 9.956711837552807E-5 0.0 46-47 0.05217317002877671 5.430933729574258E-5 0.0013667849886095217 1.0861867459148517E-4 0.0 48-49 0.06743409380888038 5.430933729574258E-5 0.0013758365448254789 1.1767023080744225E-4 0.0 50-51 0.08607124805753603 5.430933729574258E-5 0.0013758365448254789 1.3577334323935645E-4 0.0 52-53 0.10759584873908201 8.146400594361388E-5 0.001384888101041436 1.4482489945531356E-4 0.0 54-55 0.13426173335129163 9.051556215957097E-5 0.001393939657257393 1.4482489945531356E-4 0.0 56-57 0.16551675696499146 9.051556215957097E-5 0.001393939657257393 1.6292801188722776E-4 0.0 58-59 0.20289063258067833 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 60-61 0.24683593800915005 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 62-63 0.29623028027962794 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 64-65 0.35386153870662673 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 66-67 0.4206348689117423 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 68-69 0.49603433219066484 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 70-71 0.5802862174487935 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 72-73 0.6744405052071794 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 74-75 0.782950561124073 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 76-77 0.901082421298529 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 78-79 1.0315696557077665 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 80-81 1.1745751923636727 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 82-83 1.3323890749888847 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 84-85 1.5091026069930151 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 86-87 1.7007964645345544 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 88-89 1.9116343634728432 9.051556215957097E-5 0.001393939657257393 1.8103112431914193E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 16870 0.0 91.534004 1 TCGACGG 18390 0.0 83.98889 2 TATCTCG 7070 0.0 82.94991 2 CGACGGC 19355 0.0 79.703156 3 ACGGCGC 19435 0.0 79.3607 5 CGGCGCG 19595 0.0 78.63926 6 GACGGCG 19655 0.0 78.55775 4 ATCTCGA 7760 0.0 75.45172 3 ATATCTC 8290 0.0 71.227646 1 TCTCGAG 6910 0.0 68.91313 4 TCGAGGG 7065 0.0 66.83102 6 AGGGCGC 7170 0.0 66.23603 9 CTCGAGG 7185 0.0 66.000206 5 CGAGGGC 7545 0.0 62.577644 7 CGCGCCG 25855 0.0 60.156174 9 GGCGCGC 25905 0.0 59.99593 7 GCGCGCC 26000 0.0 59.84437 8 GAGGGCG 8050 0.0 58.882656 8 TCTCGAC 1990 0.0 55.165962 4 CTCGACG 2050 0.0 53.310833 5 >>END_MODULE