FastQCFastQC Report
Tue 31 May 2016
SRR2048239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523912
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT323240.5851650062491944No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT169280.3064494872474435No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85910.15552383890257485No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT56810.10284378172570453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG34950.074.916091
TCGACGG43950.061.9535872
TATCTCG43600.059.5082052
ATATCTC47150.055.531661
CGACGGC51850.052.514183
ACGGCGC54500.049.763825
ATCTCGA93150.047.901981
GACGGCG56950.047.7279744
CGGCGCG57600.047.1680226
TCTCGAG95200.043.725812
TCGAGGG106750.038.7277374
CTCGAGG107150.038.671863
GGGCGCG113700.036.4275678
CGAGGGC113550.036.3920255
AGGGCGC118750.034.8384367
GAGGGCG122750.033.7418676
GGCGCGC170650.026.0799889
CGCGCCG168300.023.5090459
GCGCGCC170100.023.2602738
TCTCGAC13200.022.3197062
Produced by FastQC (version 0.11.5)