##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048239_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5523912 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28510990037495 33.0 31.0 34.0 30.0 34.0 2 32.41926102370928 34.0 31.0 34.0 31.0 34.0 3 32.4946492630585 34.0 31.0 34.0 31.0 34.0 4 36.05114509427377 37.0 35.0 37.0 35.0 37.0 5 35.9132136790014 37.0 35.0 37.0 35.0 37.0 6 35.901879320307785 37.0 35.0 37.0 35.0 37.0 7 35.880449398904254 37.0 35.0 37.0 35.0 37.0 8 35.85140693769198 37.0 35.0 37.0 35.0 37.0 9 37.44804714484952 39.0 37.0 39.0 34.0 39.0 10-11 37.44471888762891 39.0 37.0 39.0 34.0 39.0 12-13 37.44576751041653 39.0 37.0 39.0 34.0 39.0 14-15 38.7829835087887 40.0 38.0 41.0 34.5 41.0 16-17 38.791994242486126 40.0 38.0 41.0 34.5 41.0 18-19 38.82457794403676 40.0 38.0 41.0 35.0 41.0 20-21 38.84400692842319 40.0 38.0 41.0 35.0 41.0 22-23 38.82769457587304 40.0 38.0 41.0 35.0 41.0 24-25 38.81310844560883 40.0 38.0 41.0 34.5 41.0 26-27 38.72481467843804 40.0 38.0 41.0 34.0 41.0 28-29 38.636655688939285 40.0 38.0 41.0 34.0 41.0 30-31 38.51262040380078 40.0 38.0 41.0 34.0 41.0 32-33 38.42128857230166 40.0 38.0 41.0 34.0 41.0 34-35 38.37860948907224 40.0 38.0 41.0 34.0 41.0 36-37 38.30638685047843 40.0 38.0 41.0 34.0 41.0 38-39 38.22550386392832 40.0 37.5 41.0 33.5 41.0 40-41 38.10936370818362 40.0 37.0 41.0 33.0 41.0 42-43 38.03937617036622 40.0 37.0 41.0 33.0 41.0 44-45 37.937536477771545 40.0 37.0 41.0 33.0 41.0 46-47 37.83301824866146 40.0 37.0 41.0 33.0 41.0 48-49 37.73811078091035 40.0 37.0 41.0 33.0 41.0 50-51 37.66020973904001 40.0 36.0 41.0 33.0 41.0 52-53 37.64416078677575 40.0 36.0 41.0 33.0 41.0 54-55 37.480266792809154 39.5 36.0 41.0 32.0 41.0 56-57 37.25786055244906 39.0 36.0 41.0 32.0 41.0 58-59 37.02412800928038 39.0 35.0 41.0 31.5 41.0 60-61 36.76732495014403 39.0 35.0 41.0 31.0 41.0 62-63 36.46823555480247 38.0 35.0 40.0 31.0 41.0 64-65 36.161786972710644 37.5 35.0 40.0 31.0 41.0 66-67 35.82677367054363 37.0 35.0 40.0 30.5 41.0 68-69 35.469720100537444 36.5 34.5 39.0 30.0 41.0 70-71 35.107128788438345 36.0 34.0 39.0 30.0 41.0 72-73 34.72653012937208 36.0 34.0 39.0 30.0 40.0 74-75 34.371509810438695 35.0 34.0 37.5 29.0 39.5 76-77 34.10841076034521 35.0 34.0 37.0 29.5 39.0 78-79 33.938411039133136 35.0 34.0 36.5 30.0 39.0 80-81 33.683234997226606 35.0 34.0 36.0 30.0 38.0 82-83 33.441420591059384 35.0 34.0 36.0 29.5 37.0 84-85 33.23627295293625 35.0 34.0 35.5 29.0 37.0 86-87 33.038154753370435 35.0 34.0 35.0 29.0 36.0 88-89 32.89951604949536 35.0 34.0 35.0 29.0 36.0 90-91 32.77042402196125 35.0 34.0 35.0 29.0 36.0 92-93 32.638897668898416 35.0 33.0 35.0 29.0 36.0 94-95 32.52903875007422 35.0 33.0 35.0 29.0 35.0 96-97 32.420721854366974 35.0 33.0 35.0 29.0 35.0 98-99 32.27893583388005 35.0 33.0 35.0 29.0 35.0 100-101 31.834070039493753 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3630124141678266 1101 2 0.3712738705698868 1101 3 0.3398138985931567 1101 4 0.2388635076924217 1101 5 0.2476308846601043 1101 6 0.3089809961632355 1101 7 0.30904207344552503 1101 8 0.3085320488954011 1101 9 0.38811494211395825 1101 10-11 0.403430027528934 1101 12-13 0.4082852830861867 1101 14-15 0.5431710420661204 1101 16-17 0.5337993420209202 1101 18-19 0.5571058421611284 1101 20-21 0.5019882996260847 1101 22-23 0.5763322058718643 1101 24-25 0.5321319318506781 1101 26-27 0.5472635062908324 1101 28-29 0.533559742551212 1101 30-31 0.5379058706605164 1101 32-33 0.5323103705670675 1101 34-35 0.5362766667510073 1101 36-37 0.5489552685095518 1101 38-39 0.5881302007878091 1101 40-41 0.5746688532217448 1101 42-43 0.47195805409845093 1101 44-45 0.5805688508680191 1101 46-47 0.5736031993137871 1101 48-49 0.5241715688746424 1101 50-51 0.5404838391687861 1101 52-53 0.5106179283105092 1101 54-55 0.5392780601902842 1101 56-57 0.5395582175151858 1101 58-59 0.4474068220298051 1101 60-61 0.4046926684865326 1101 62-63 0.46527857436660724 1101 64-65 0.43998477586342233 1101 66-67 0.503323356112169 1101 68-69 0.4382468868480487 1101 70-71 0.4266133512768491 1101 72-73 0.409150454082436 1101 74-75 0.38336148217288724 1101 76-77 0.4717788656394504 1101 78-79 0.4621625469573658 1101 80-81 0.3972527392787626 1101 82-83 0.43278294229794767 1101 84-85 0.3846894607541316 1101 86-87 0.3509992736655647 1101 88-89 0.3857750365886261 1101 90-91 0.3970523991658723 1101 92-93 0.4360240934359325 1101 94-95 0.4517957171322564 1101 96-97 0.5173485272191556 1101 98-99 0.5124221592475351 1101 100-101 0.5020942753006246 1102 1 0.2840357672612015 1102 2 0.3330216499581624 1102 3 0.2948532367057055 1102 4 0.22413915512533578 1102 5 0.24766580451239406 1102 6 0.24323565523015134 1102 7 0.2390732867360157 1102 8 0.2592509140320658 1102 9 0.31580743155095803 1102 10-11 0.3623980025017062 1102 12-13 0.34799632620517684 1102 14-15 0.4457815692453835 1102 16-17 0.4729341564178924 1102 18-19 0.4620864026637861 1102 20-21 0.4742136864142381 1102 22-23 0.49815987030556386 1102 24-25 0.45316983610059225 1102 26-27 0.4653880512109012 1102 28-29 0.503347032268735 1102 30-31 0.4649539068419841 1102 32-33 0.4559580477625147 1102 34-35 0.47660235189123057 1102 36-37 0.5114614474524402 1102 38-39 0.5086948087559691 1102 40-41 0.5264804510153311 1102 42-43 0.5096938365514774 1102 44-45 0.5122102800848083 1102 46-47 0.5388975362492232 1102 48-49 0.5190844743194845 1102 50-51 0.48337352432969993 1102 52-53 0.450756911368984 1102 54-55 0.4311837816757986 1102 56-57 0.41170454277590096 1102 58-59 0.3812542972016999 1102 60-61 0.35677345171323793 1102 62-63 0.4072938892286402 1102 64-65 0.3383984016161037 1102 66-67 0.39004489006688203 1102 68-69 0.3915449097646544 1102 70-71 0.34709872816115706 1102 72-73 0.3574600175059359 1102 74-75 0.3576944917996272 1102 76-77 0.30991037523562426 1102 78-79 0.3032323742960088 1102 80-81 0.266368565110767 1102 82-83 0.26719297351299076 1102 84-85 0.2407175554343155 1102 86-87 0.27702899930309854 1102 88-89 0.2701484772359777 1102 90-91 0.1843562000975183 1102 92-93 0.25521294984892506 1102 94-95 0.2544209138652107 1102 96-97 0.30064730320013666 1102 98-99 0.36888261548936896 1102 100-101 0.3729649004490021 1103 1 0.3336112817395289 1103 2 0.34992133694424155 1103 3 0.3150874621235147 1103 4 0.20490173308014192 1103 5 0.25259398168678615 1103 6 0.25257828444318875 1103 7 0.25942126928701725 1103 8 0.2625090949360356 1103 9 0.36616366279271517 1103 10-11 0.38104780921846526 1103 12-13 0.3838086560239873 1103 14-15 0.5135631597051571 1103 16-17 0.46245369615340337 1103 18-19 0.44822855025302033 1103 20-21 0.4858491192159775 1103 22-23 0.52659123216025 1103 24-25 0.5341319305616068 1103 26-27 0.5244189131575254 1103 28-29 0.4589086392882251 1103 30-31 0.4443541262530033 1103 32-33 0.4674188192124902 1103 34-35 0.4769497266986491 1103 36-37 0.4783086451311718 1103 38-39 0.4776414488689156 1103 40-41 0.4754380409016363 1103 42-43 0.4382614991904177 1103 44-45 0.4781297597731822 1103 46-47 0.4549847687511459 1103 48-49 0.48662577231434767 1103 50-51 0.5138648512573099 1103 52-53 0.4931620903832368 1103 54-55 0.44529174394790516 1103 56-57 0.41555631255639724 1103 58-59 0.3370699098747636 1103 60-61 0.3048337107886141 1103 62-63 0.3557300922544755 1103 64-65 0.25854232256995147 1103 66-67 0.28707832219750884 1103 68-69 0.24084099205209952 1103 70-71 0.24969924739234273 1103 72-73 0.27223527704361317 1103 74-75 0.18043581535089004 1103 76-77 0.2227064424593408 1103 78-79 0.21003645334631216 1103 80-81 0.211987331193896 1103 82-83 0.17129389219275026 1103 84-85 0.21183739831441528 1103 86-87 0.1318187979373917 1103 88-89 0.0966676803750488 1103 90-91 0.14033543604436005 1103 92-93 0.17457082978995686 1103 94-95 0.1623612656500555 1103 96-97 0.16865760263591767 1103 98-99 0.22452156697065107 1103 100-101 0.2204313888442222 1104 1 0.2298506351056986 1104 2 0.24467314757478675 1104 3 0.2057777862141421 1104 4 0.15825685213670937 1104 5 0.1408173520340199 1104 6 0.18331333401761896 1104 7 0.18676035086318876 1104 8 0.23953910485222707 1104 9 0.3234777409849414 1104 10-11 0.2892443322802549 1104 12-13 0.3034977279557012 1104 14-15 0.391008645796191 1104 16-17 0.32564889633596295 1104 18-19 0.369059714081466 1104 20-21 0.3716676719178835 1104 22-23 0.4279700381454816 1104 24-25 0.3600093300709588 1104 26-27 0.35523635985962443 1104 28-29 0.3569945447461862 1104 30-31 0.3717227906947116 1104 32-33 0.38249951598270826 1104 34-35 0.29534998055274997 1104 36-37 0.37407445235839276 1104 38-39 0.3188758162822225 1104 40-41 0.3360939894609132 1104 42-43 0.3535261739545632 1104 44-45 0.3934236123528194 1104 46-47 0.42429628714078405 1104 48-49 0.3389322324390349 1104 50-51 0.3736815645487894 1104 52-53 0.37746989169257006 1104 54-55 0.38548475469995225 1104 56-57 0.4052174101686816 1104 58-59 0.29647344227499417 1104 60-61 0.32177773582861136 1104 62-63 0.33694284079673764 1104 64-65 0.2982242724427806 1104 66-67 0.23015629833894735 1104 68-69 0.22778524136190725 1104 70-71 0.1976923565957165 1104 72-73 0.1579601921238094 1104 74-75 0.1529154433233657 1104 76-77 0.18946469591538317 1104 78-79 0.17604878937992652 1104 80-81 0.15830911153351224 1104 82-83 0.1716703044885577 1104 84-85 0.13271717525435633 1104 86-87 0.13717253624231773 1104 88-89 0.1296221469514265 1104 90-91 0.14861533403882987 1104 92-93 0.158647161342941 1104 94-95 0.13123243068597645 1104 96-97 0.14442099974431244 1104 98-99 0.14862576966190488 1104 100-101 0.08453381585291808 1105 1 0.18141881601017218 1105 2 0.24464696207932235 1105 3 0.1729814474442577 1105 4 0.12682990492006496 1105 5 0.1790293046099336 1105 6 0.163145218130488 1105 7 0.1597353203246854 1105 8 0.17333439871933365 1105 9 0.1997754857268106 1105 10-11 0.19628927213906877 1105 12-13 0.19977607651841112 1105 14-15 0.3086783052438875 1105 16-17 0.2650970170635816 1105 18-19 0.27868844477927013 1105 20-21 0.2466807559742037 1105 22-23 0.31756144137084164 1105 24-25 0.30087853193580827 1105 26-27 0.3267632005000536 1105 28-29 0.217082612561029 1105 30-31 0.2621443144544102 1105 32-33 0.2641231721734627 1105 34-35 0.32028234778452713 1105 36-37 0.3080635717357296 1105 38-39 0.31090067111507835 1105 40-41 0.3526713271536366 1105 42-43 0.2929177935307905 1105 44-45 0.2880515237630661 1105 46-47 0.27219011181247055 1105 48-49 0.3599571480283572 1105 50-51 0.3547089145687181 1105 52-53 0.34703332079876503 1105 54-55 0.286447150228625 1105 56-57 0.3465454561735015 1105 58-59 0.3019225145526434 1105 60-61 0.31765509865412866 1105 62-63 0.13033482463364976 1105 64-65 0.1517602370109472 1105 66-67 0.2920696147814539 1105 68-69 0.19978622998604578 1105 70-71 0.18242395297268388 1105 72-73 0.14982954711052798 1105 74-75 0.09555724887155037 1105 76-77 0.2068361855901415 1105 78-79 0.17645177900978837 1105 80-81 0.1930440322029412 1105 82-83 0.10845511835856314 1105 84-85 0.10224452455810962 1105 86-87 0.13762518514159439 1105 88-89 0.08801228215911294 1105 90-91 0.08603516355665164 1105 92-93 0.045533239933448044 1105 94-95 0.03313491748618702 1105 96-97 0.10574102489407267 1105 98-99 0.11004542624142744 1105 100-101 0.04535336846086935 1106 1 0.1486047119092646 1106 2 0.13450559300810028 1106 3 0.10452437451502306 1106 4 0.1127116000971995 1106 5 0.08460944132382053 1106 6 0.08669358998047727 1106 7 0.12864362375943017 1106 8 0.1414774181887566 1106 9 0.22765173682976325 1106 10-11 0.17596502592066088 1106 12-13 0.20849621633611548 1106 14-15 0.2486479045847858 1106 16-17 0.23525454898422282 1106 18-19 0.2147381518052569 1106 20-21 0.1899218042750661 1106 22-23 0.20685676293857824 1106 24-25 0.19915209344993912 1106 26-27 0.17862956298929333 1106 28-29 0.1209630251556959 1106 30-31 0.17823829603829466 1106 32-33 0.22720670509509944 1106 34-35 0.18879450765497552 1106 36-37 0.21446107749817855 1106 38-39 0.18950625303609314 1106 40-41 0.211502772990805 1106 42-43 0.15920840223573407 1106 44-45 0.12876479070897773 1106 46-47 0.14805984728369737 1106 48-49 0.1937326181628407 1106 50-51 0.1952268641473296 1106 52-53 0.054770475510864 1106 54-55 0.10329394464427821 1106 56-57 0.18043790928289383 1106 58-59 0.17377851914041997 1106 60-61 0.10020015411541294 1106 62-63 0.13707520250601135 1106 64-65 0.13582743513393325 1106 66-67 0.18812222848455207 1106 68-69 0.10698945802195681 1106 70-71 0.11226533230228597 1106 72-73 0.0881823801824595 1106 74-75 0.07371715928330502 1106 76-77 0.1422747148738921 1106 78-79 0.20285046246592486 1106 80-81 0.09542936631257959 1106 82-83 0.12125487672390989 1106 84-85 0.1709727409194528 1106 86-87 0.15484521327771006 1106 88-89 0.1299485431961216 1106 90-91 0.17641386705825113 1106 92-93 0.26016951575739 1106 94-95 0.18260075489161665 1106 96-97 0.25201515781877504 1106 98-99 0.25311239796411655 1106 100-101 0.26539548341651553 1107 1 0.014112842853812424 1107 2 0.0721519395536987 1107 3 0.044292065509594636 1107 4 0.025727422825383428 1107 5 0.07073361671136524 1107 6 0.0902166058280045 1107 7 0.07901577328283338 1107 8 0.06428211490362656 1107 9 0.0809292526752401 1107 10-11 -0.06653357165336615 1107 12-13 0.01048760192650633 1107 14-15 0.006232458150883247 1107 16-17 -0.0901121703156349 1107 18-19 -0.023435279455718216 1107 20-21 -0.03873803703628198 1107 22-23 0.09358995339190557 1107 24-25 0.006151533018531552 1107 26-27 -0.09502888344180604 1107 28-29 0.0028002425226674177 1107 30-31 -0.0028632702160322765 1107 32-33 -0.022929899386930686 1107 34-35 -0.028469571634587965 1107 36-37 -0.031132114192651272 1107 38-39 -0.05130244010771179 1107 40-41 -0.005284874924171845 1107 42-43 -0.005358995704625613 1107 44-45 -0.009648373468003513 1107 46-47 0.03341116037912428 1107 48-49 0.044296194519382937 1107 50-51 0.07819630333432315 1107 52-53 0.13458759312772628 1107 54-55 0.06224373186574894 1107 56-57 0.16197200968537828 1107 58-59 -0.01099738159757635 1107 60-61 0.12003826985426258 1107 62-63 -0.1443419284559937 1107 64-65 -0.0066077864763229854 1107 66-67 -0.015319317840457813 1107 68-69 0.07411639384948643 1107 70-71 0.1232092539121794 1107 72-73 0.09273902493276864 1107 74-75 0.09361154453456777 1107 76-77 0.0775018631457769 1107 78-79 0.0020472502097774736 1107 80-81 0.00654036633392252 1107 82-83 0.04491419360771687 1107 84-85 0.08918373800874235 1107 86-87 -0.06426977724379412 1107 88-89 0.022391324000935242 1107 90-91 0.028023145469667554 1107 92-93 0.028795034283106702 1107 94-95 0.05713003670507533 1107 96-97 0.05036500585875814 1107 98-99 -0.061273991626592306 1107 100-101 -0.06784054606008283 1108 1 -0.03218797005834517 1108 2 0.0362390142278457 1108 3 -0.013232971049504272 1108 4 -0.02214379223313756 1108 5 -0.0317268400608981 1108 6 -0.02604363069358584 1108 7 -0.026970676964971574 1108 8 -0.05032550374805567 1108 9 -0.24044284874094757 1108 10-11 0.06114976095916802 1108 12-13 0.0691620354951823 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-0.23701066326291453 2214 96-97 -0.2802935785335521 2214 98-99 -0.33660600079294767 2214 100-101 -0.5048611447312261 2215 1 -0.5714592497568596 2215 2 -0.939760288288717 2215 3 -0.5473482292095149 2215 4 -0.3060195382974058 2215 5 -0.31151945029736794 2215 6 -0.31980405790113053 2215 7 -0.30527878210504866 2215 8 -0.4188404314705281 2215 9 -0.41720094471637736 2215 10-11 -0.5296029222497509 2215 12-13 -0.42074169082674473 2215 14-15 -0.5312846484506366 2215 16-17 -0.4519826279787367 2215 18-19 -0.46812217408250234 2215 20-21 -0.49973549754734137 2215 22-23 -0.513161491527832 2215 24-25 -0.5000657405181954 2215 26-27 -0.5486895021562077 2215 28-29 -0.47665809998574105 2215 30-31 -0.5006067535938072 2215 32-33 -0.5522205858831839 2215 34-35 -0.6106140790210475 2215 36-37 -0.5189788687913222 2215 38-39 -0.5117171013261341 2215 40-41 -0.5862072916562369 2215 42-43 -0.556796129422338 2215 44-45 -0.5262721719101151 2215 46-47 -0.5454509664754639 2215 48-49 -0.6155113878076008 2215 50-51 -0.6119346062912001 2215 52-53 -0.5503332468383348 2215 54-55 -0.6079722009510178 2215 56-57 -0.5433133454577401 2215 58-59 -0.5543220561369679 2215 60-61 -0.5404679386955422 2215 62-63 -0.5138755696515744 2215 64-65 -0.5051211544965639 2215 66-67 -0.45198668968119904 2215 68-69 -0.4582326247971835 2215 70-71 -0.4284318737045538 2215 72-73 -0.4379498166966158 2215 74-75 -0.48860956329524896 2215 76-77 -0.3917428668568874 2215 78-79 -0.3048256385119785 2215 80-81 -0.26489561194380684 2215 82-83 -0.3467193929308081 2215 84-85 -0.37256835576814495 2215 86-87 -0.34857448853954054 2215 88-89 -0.30143954615422786 2215 90-91 -0.2484544636770991 2215 92-93 -0.26188223974295965 2215 94-95 -0.3159246717613584 2215 96-97 -0.34277301715972186 2215 98-99 -0.3557161259096233 2215 100-101 -0.3259270900237645 2216 1 -0.4526358476321626 2216 2 -0.4589887071318053 2216 3 -0.4614247305648007 2216 4 -0.3156108365259058 2216 5 -0.38166231874563294 2216 6 -0.31326781601558196 2216 7 -0.37649987381566064 2216 8 -0.4383735868154943 2216 9 -0.4738479267479505 2216 10-11 -0.6662934059765249 2216 12-13 -0.7011851454461464 2216 14-15 -0.9038866475492142 2216 16-17 -1.2232704768559017 2216 18-19 -1.1831029852461654 2216 20-21 -1.6340041501545954 2216 22-23 -1.4981619308964227 2216 24-25 -1.5354098437129196 2216 26-27 -1.3452698227897386 2216 28-29 -1.3745998311109986 2216 30-31 -1.3996875128498587 2216 32-33 -1.1029086297645918 2216 34-35 -0.716038224618714 2216 36-37 -1.054710800561928 2216 38-39 -1.3287044107498431 2216 40-41 -1.3481958616703338 2216 42-43 -0.6927561883083158 2216 44-45 -1.2046933047605037 2216 46-47 -0.829820435114577 2216 48-49 -0.8104602334483744 2216 50-51 -1.0222548928327626 2216 52-53 -0.99221351866791 2216 54-55 -1.2208156053475747 2216 56-57 -1.2872529618622437 2216 58-59 -0.9456563429774505 2216 60-61 -0.9910476135834045 2216 62-63 -0.8561880084380533 2216 64-65 -0.9164592460329857 2216 66-67 -0.7338255770322704 2216 68-69 -0.6704426059221049 2216 70-71 -0.5971962438991909 2216 72-73 -0.6000745122958477 2216 74-75 -0.5888932659925601 2216 76-77 -0.5024273219962936 2216 78-79 -0.44628516371229665 2216 80-81 -0.4726858353782646 2216 82-83 -0.6388159632432249 2216 84-85 -0.4892762842861984 2216 86-87 -0.5637155424050491 2216 88-89 -0.5883620460662655 2216 90-91 -0.6010722865559686 2216 92-93 -0.531419579873635 2216 94-95 -0.6730384330649244 2216 96-97 -0.6525470040054628 2216 98-99 -0.9419443949685871 2216 100-101 -0.6862992258567289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 15.0 12 59.0 13 240.0 14 827.0 15 1806.0 16 3001.0 17 4997.0 18 8625.0 19 18255.0 20 22131.0 21 20889.0 22 20150.0 23 20268.0 24 21722.0 25 25317.0 26 31742.0 27 41321.0 28 54497.0 29 71459.0 30 93804.0 31 123128.0 32 160398.0 33 212766.0 34 289037.0 35 411304.0 36 669601.0 37 1268249.0 38 1564086.0 39 363705.0 40 511.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.895436422593264 40.80834379693232 12.52335301503717 21.77286676543725 2 24.42111677376468 27.656939502294755 30.24296911319369 17.678974610746874 3 22.53985943295259 27.334957544580725 24.847209731074642 25.27797329139204 4 23.368501616004806 26.675923574907607 26.413338640933258 23.54223616815433 5 23.661130010760488 27.31345104701161 25.25695557785859 23.76846336436931 6 23.988506695979225 25.769617618817968 26.316856604522304 23.925019080680503 7 24.231758217726856 27.32431291447076 24.918771334518 23.525157533284382 8 24.011678679892075 25.479605757658703 26.72166030161234 23.787055260836883 9 23.475518798996074 25.324751733916106 26.865127467635258 24.334601999452563 10-11 24.401827907468476 25.79923431075658 25.78736772055746 24.011570061217487 12-13 24.217710202479694 25.953391364670548 25.582983581201148 24.245914851648614 14-15 23.883762087448172 26.03254722377909 25.748500338166142 24.3351903506066 16-17 23.99042562589701 26.240054150029906 25.724088291051707 24.04543193302138 18-19 23.651745687815602 26.288409942845753 26.265283214620656 23.794561154717993 20-21 23.01655964106597 26.359951063666475 26.664608342783158 23.9588809524844 22-23 22.847585189626482 26.272703113300867 27.168064951070907 23.711646746001747 24-25 22.83474103135604 25.900711307493673 28.05250156048829 23.212046100661997 26-27 22.73807001224043 25.810733004031295 28.547935876165877 22.903261107562393 28-29 22.820257148169127 25.909833221003215 28.48096190062921 22.788947730198444 30-31 22.83462051023455 25.899261039801424 28.40329328290167 22.862825167062358 32-33 22.769209825320335 25.897355524251246 28.517011903612143 22.816422746816272 34-35 22.85441911456953 26.019621601502703 28.41172162047477 22.714237663453 36-37 22.86451366934463 25.982195768342777 28.281798142493773 22.871492419818818 38-39 22.866608718588036 26.000430311099354 28.31623539157631 22.816725578736296 40-41 22.92775747310735 26.18427500680225 27.991781903641755 22.896185616448648 42-43 23.00731031165518 26.178651188981284 27.835647922906777 22.978390576456764 44-45 22.955723703891774 26.231728803556546 27.878004504975433 22.934542987576243 46-47 23.009784832527487 26.439839269545928 27.6946034477593 22.855772450167287 48-49 23.08057433577638 26.38761500795225 27.51622650000819 23.015584156263184 50-51 22.96595983834976 26.399040605629914 27.63288773342537 23.002111822594955 52-53 23.048401973625253 26.525880070856438 27.491004906908138 22.93471304861016 54-55 23.09051169061175 26.47653436987988 27.361287781695232 23.07166615781314 56-57 23.124843464465496 26.4818885840824 27.356758728041235 23.036509223410864 58-59 23.17997489249111 26.593973347009797 27.225462768645503 23.00058899185359 60-61 23.21413555474031 26.551848956346223 27.064531467111063 23.169484021802404 62-63 23.235172560241438 26.565656455940434 27.0902227177661 23.10894826605203 64-65 23.282775445387397 26.725687779591915 26.93426328033876 23.057273494681926 66-67 23.315188864110727 26.666178485493642 26.78170363341661 23.23692901697902 68-69 23.283591438375353 26.678731250842365 26.803326610101973 23.23435070068031 70-71 23.34517885041158 26.812735228254002 26.688972807206518 23.15311311412791 72-73 23.39117793294767 26.707651528324277 26.584351173747507 23.316819364980546 74-75 23.397666888069534 26.72023059208341 26.673877195088153 23.208225324758907 76-77 23.38850875559719 26.8939066503508 26.511991193125322 23.205593400926688 78-79 23.39857131827158 26.81075076709794 26.465134664282242 23.325543250348236 80-81 23.33572173773256 26.804993780674597 26.571291608427433 23.287992873165408 82-83 23.386001363920837 26.967506496003374 26.448287866589933 23.19820427348586 84-85 23.43313036123675 26.902220745008247 26.368215134491642 23.296433759263362 86-87 23.41362849618685 26.88339837481089 26.466474568471504 23.236498560530755 88-89 23.40950255119359 27.005305143028863 26.350095992222865 23.23509631355468 90-91 23.448125418635385 26.93051105197415 26.291125835007872 23.330237694382593 92-93 23.400592119174032 26.903161324052917 26.404852886227957 23.291393670545094 94-95 23.433872588846455 27.071729238264474 26.253712948359787 23.240685224529283 96-97 23.4689029407806 27.008020886728296 26.198193103212308 23.324883069278798 98-99 23.419849036294124 27.011190713535278 26.2704835641345 23.298476686036096 100-101 23.46536825124078 27.137873327551322 26.117541924201188 23.279216497006715 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1596.0 1 1139.0 2 516.0 3 283.0 4 189.5 5 158.0 6 153.5 7 153.5 8 164.5 9 174.0 10 189.5 11 198.0 12 220.5 13 346.5 14 478.0 15 624.5 16 2936.0 17 3656.5 18 2420.5 19 3031.5 20 4029.0 21 5393.0 22 7302.5 23 9848.5 24 12739.5 25 16406.5 26 21103.5 27 26500.0 28 32655.5 29 39723.5 30 48128.0 31 57303.0 32 66506.0 33 77014.5 34 87285.5 35 98468.5 36 112325.0 37 126227.5 38 140220.5 39 154325.5 40 168226.0 41 179546.5 42 190541.0 43 202532.0 44 211408.0 45 218537.5 46 225581.0 47 229727.0 48 229600.0 49 227828.5 50 224202.0 51 220509.0 52 217745.0 53 211634.0 54 200685.5 55 185905.0 56 167882.0 57 148651.5 58 132000.0 59 115106.5 60 94630.0 61 76131.0 62 61938.5 63 49657.5 64 39490.0 65 30895.5 66 23430.0 67 17567.0 68 13364.0 69 10751.5 70 8862.0 71 7299.0 72 6001.0 73 4780.5 74 3707.5 75 2528.5 76 1514.5 77 989.0 78 626.5 79 335.0 80 117.5 81 48.0 82 31.5 83 14.0 84 5.5 85 3.0 86 3.5 87 2.5 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.041094065220445224 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 9.051556215957097E-6 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1767023080744227E-4 28-29 2.715466864787129E-4 30-31 2.715466864787129E-5 32-33 9.051556215957097E-6 34-35 0.0 36-37 9.051556215957097E-6 38-39 2.715466864787129E-5 40-41 9.051556215957097E-5 42-43 4.525778107978548E-5 44-45 3.530106924223268E-4 46-47 9.956711837552807E-5 48-49 9.051556215957097E-6 50-51 1.9008268053509903E-4 52-53 0.001212908532938251 54-55 0.001022825852403152 56-57 1.1767023080744227E-4 58-59 0.0012038569767222939 60-61 4.6162936701381194E-4 62-63 2.715466864787129E-4 64-65 2.3534046161488453E-4 66-67 1.1767023080744227E-4 68-69 5.52144929173383E-4 70-71 3.9826847350211226E-4 72-73 4.525778107978548E-5 74-75 8.146400594361388E-4 76-77 8.236916156520958E-4 78-79 1.5387645567127066E-4 80-81 1.8103112431914194E-5 82-83 0.0023715077285807597 84-85 0.0 86-87 1.8103112431914194E-5 88-89 4.887840356616833E-4 90-91 2.1723734918297033E-4 92-93 4.3447469836594067E-4 94-95 0.0 96-97 3.892169172861552E-4 98-99 2.715466864787129E-5 100-101 0.008354586387328401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5523912.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.16830583335032 #Duplication Level Percentage of deduplicated Percentage of total 1 81.54677390356902 54.773586492779934 2 10.73313649444423 14.418531892198471 3 3.32822457709867 6.7065361882990935 4 1.4502356645833174 3.8963949059665723 5 0.8167953047995021 2.743137841800878 6 0.48819065813079565 1.9674563658188315 7 0.3417452331553113 1.6068113836365892 8 0.24015887484573625 1.2904851803385373 9 0.17726869643171983 1.0716154214944584 >10 0.866752425777847 9.607626589846848 >50 0.009319968076957987 0.38344384484852423 >100 0.001101275040774592 0.15737554186238703 >500 5.3986190703381866E-5 0.034938060924337634 >1k 1.3496547526811733E-4 0.1903190562984529 >5k 5.398619010566001E-5 0.25876284380740566 >10k+ 5.398619010566001E-5 0.8929783900786396 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32324 0.5851650062491944 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 16928 0.3064494872474435 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8591 0.15552383890257485 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 5681 0.10284378172570453 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.8103112431914194E-5 0.0 0.0 1.8103112431914194E-5 0.0 2 1.8103112431914194E-5 0.0 0.0 1.8103112431914194E-5 0.0 3 5.430933729574258E-5 0.0 0.0 1.8103112431914194E-5 0.0 4 7.241244972765678E-5 0.0 0.0 1.8103112431914194E-5 0.0 5 1.0861867459148517E-4 0.0 0.0 1.8103112431914194E-5 0.0 6 1.4482489945531356E-4 0.0 0.0 1.8103112431914194E-5 0.0 7 1.9913423675105613E-4 0.0 0.0 1.8103112431914194E-5 0.0 8 2.715466864787129E-4 0.0 0.0 1.8103112431914194E-5 0.0 9 4.3447469836594067E-4 0.0 0.0 1.8103112431914194E-5 0.0 10-11 8.689493967318813E-4 0.0 0.0 2.715466864787129E-5 0.0 12-13 0.00140299121347335 0.0 0.0 3.620622486382839E-5 0.0 14-15 0.0018374659118392906 0.0 0.0 3.620622486382839E-5 0.0 16-17 0.0022900437226371457 0.0 0.0 3.620622486382839E-5 0.0 18-19 0.002606848190195644 0.0 0.0 3.620622486382839E-5 0.0 20-21 0.003077529113425413 8.146400594361388E-5 0.0 3.620622486382839E-5 0.0 22-23 0.0038740660604296377 5.068871480935974E-4 0.0 3.620622486382839E-5 0.0 24-25 0.004788273238241304 8.598978405159242E-4 0.0 3.620622486382839E-5 0.0 26-27 0.005639119522541272 9.32310290243581E-4 0.0 3.620622486382839E-5 0.0 28-29 0.006571429812784852 0.0010047227399712377 0.0 5.430933729574258E-5 0.0 30-31 0.007956317913826288 0.0010590320772669803 0.0 5.430933729574258E-5 0.0 32-33 0.009440773133243253 0.001131444526994637 0.0 5.430933729574258E-5 0.0 34-35 0.011423063944537857 0.0011404960832105942 0.0 5.430933729574258E-5 0.0 36-37 0.014545850839043055 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 38-39 0.019071628947021604 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 40-41 0.02437584088957246 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 42-43 0.03170760142449771 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 44-45 0.04006218781182611 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 46-47 0.05167533443689907 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 48-49 0.06687289732349103 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 50-51 0.08546479379106692 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 52-53 0.10692603357910119 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 54-55 0.13351045418536717 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 56-57 0.1645210857812362 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 58-59 0.20185875517205923 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 60-61 0.245577771695132 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 62-63 0.2947005672791312 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 64-65 0.35194260878884387 0.0011585991956425084 0.0 5.430933729574258E-5 0.0 66-67 0.4179827629404668 0.0011767023080744227 0.0 5.430933729574258E-5 0.0 68-69 0.49275766884048844 0.0011767023080744227 0.0 5.430933729574258E-5 0.0 70-71 0.5765388731753873 0.0011767023080744227 0.0 6.336089351169968E-5 0.0 72-73 0.6699237786554166 0.0011948054205063367 0.0 7.241244972765678E-5 0.0 74-75 0.7770127402464051 0.0011948054205063367 0.0 7.241244972765678E-5 0.0 76-77 0.8939588465565708 0.0011948054205063367 0.0 7.241244972765678E-5 0.0 78-79 1.0225090479355936 0.0011948054205063367 0.0 7.241244972765678E-5 0.0 80-81 1.1636047062299326 0.0011948054205063367 0.0 7.241244972765678E-5 0.0 82-83 1.3197802571800565 0.0011948054205063367 0.0 7.241244972765678E-5 0.0 84-85 1.4948101997280188 0.0011948054205063367 0.0 7.241244972765678E-5 0.0 86-87 1.6845308180144796 0.0011948054205063367 0.0 9.051556215957097E-5 0.0 88-89 1.8926170438631171 0.0011948054205063367 0.0 9.051556215957097E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3495 0.0 74.91609 1 TCGACGG 4395 0.0 61.953587 2 TATCTCG 4360 0.0 59.508205 2 ATATCTC 4715 0.0 55.53166 1 CGACGGC 5185 0.0 52.51418 3 ACGGCGC 5450 0.0 49.76382 5 ATCTCGA 9315 0.0 47.90198 1 GACGGCG 5695 0.0 47.727974 4 CGGCGCG 5760 0.0 47.168022 6 TCTCGAG 9520 0.0 43.72581 2 TCGAGGG 10675 0.0 38.727737 4 CTCGAGG 10715 0.0 38.67186 3 GGGCGCG 11370 0.0 36.427567 8 CGAGGGC 11355 0.0 36.392025 5 AGGGCGC 11875 0.0 34.838436 7 GAGGGCG 12275 0.0 33.741867 6 GGCGCGC 17065 0.0 26.079988 9 CGCGCCG 16830 0.0 23.509045 9 GCGCGCC 17010 0.0 23.260273 8 TCTCGAC 1320 0.0 22.319706 2 >>END_MODULE