FastQCFastQC Report
Tue 31 May 2016
SRR2048238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5637428
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT135760.2408190401722204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG179000.088.9093861
TCGACGG191900.083.020442
TATCTCG77450.080.6361852
CGACGGC198950.080.02793
CGGCGCG203550.078.02136
ACGGCGC204550.077.702175
GACGGCG205900.077.302174
ATCTCGA80950.077.0295943
ATATCTC85000.073.897351
TCTCGAG70000.069.795584
AGGGCGC70000.069.679449
TCGAGGG71300.068.360576
CTCGAGG72400.067.466945
CGAGGGC75350.064.810627
GAGGGCG78900.062.1917348
CGCGCCG264050.060.416589
GGCGCGC265950.059.999327
GCGCGCC265950.059.9955988
CTCGACG22850.058.8486825
TCTCGAC25100.053.774644
Produced by FastQC (version 0.11.5)