##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048238_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5637428 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.131310945345998 31.0 31.0 34.0 28.0 34.0 2 31.429660653759125 31.0 31.0 34.0 28.0 34.0 3 31.478587220980916 31.0 31.0 34.0 28.0 34.0 4 35.14331074383566 37.0 35.0 37.0 32.0 37.0 5 35.01478298259419 37.0 35.0 37.0 32.0 37.0 6 35.219565908424904 37.0 35.0 37.0 32.0 37.0 7 35.16336315071341 37.0 35.0 37.0 32.0 37.0 8 35.180350862130744 37.0 35.0 37.0 32.0 37.0 9 36.77512830319075 39.0 37.0 39.0 32.0 39.0 10-11 36.72012511734074 39.0 37.0 39.0 32.0 39.0 12-13 36.704736184657264 39.0 37.0 39.0 32.0 39.0 14-15 37.914071718521285 40.0 38.0 41.0 32.0 41.0 16-17 37.91573542047898 40.0 37.5 41.0 32.0 41.0 18-19 37.91586056620147 40.0 38.0 41.0 32.0 41.0 20-21 37.884885713839715 40.0 38.0 41.0 32.0 41.0 22-23 37.92769841140321 40.0 38.0 41.0 32.0 41.0 24-25 37.91488210581137 40.0 37.5 41.0 32.0 41.0 26-27 37.91127895558046 40.0 38.0 41.0 32.0 41.0 28-29 37.71214896225726 40.0 37.0 41.0 32.0 41.0 30-31 37.57683299902012 40.0 36.5 41.0 32.0 41.0 32-33 37.50573000666262 40.0 36.0 41.0 31.5 41.0 34-35 37.24802835619364 39.5 35.5 41.0 30.5 41.0 36-37 37.1709680371971 40.0 35.0 41.0 30.5 41.0 38-39 37.036704504252654 39.5 35.0 41.0 30.0 41.0 40-41 36.68707431828841 39.0 35.0 41.0 29.5 41.0 42-43 36.49196894399361 39.0 35.0 40.0 29.0 41.0 44-45 36.352596077502014 39.0 35.0 40.0 29.0 41.0 46-47 36.2617269790408 39.0 35.0 40.0 29.0 41.0 48-49 36.14186194484435 38.5 34.5 40.0 28.5 41.0 50-51 36.08859297183042 38.0 34.0 40.0 28.0 41.0 52-53 35.910507947950734 38.0 34.0 40.0 28.0 41.0 54-55 35.848521435661794 38.0 34.0 40.0 28.0 41.0 56-57 35.77925545124479 38.0 34.0 40.0 28.0 41.0 58-59 35.6652139947508 38.0 34.0 40.0 28.0 41.0 60-61 35.43977927522977 38.0 34.0 40.0 28.0 41.0 62-63 35.14196332086192 37.0 34.0 40.0 28.0 41.0 64-65 34.81193968242255 37.0 33.0 40.0 27.0 41.0 66-67 34.48668257936066 36.0 33.0 39.0 27.0 41.0 68-69 34.1449653104217 36.0 33.0 39.0 26.0 41.0 70-71 33.766195062712995 35.0 33.0 39.0 26.0 40.0 72-73 33.47614585942384 35.0 32.5 37.5 26.0 40.0 74-75 33.43296171587468 35.0 33.0 37.0 26.5 39.5 76-77 33.14921343563057 35.0 33.0 37.0 26.5 39.0 78-79 32.84577266441363 35.0 33.0 36.0 26.0 39.0 80-81 32.5608885825238 35.0 33.0 36.0 26.0 37.5 82-83 32.28208049841169 35.0 32.0 35.5 26.0 37.0 84-85 32.059832959285686 35.0 32.0 35.0 26.0 37.0 86-87 31.864464876535898 35.0 32.0 35.0 25.5 36.0 88-89 31.686069604791406 35.0 32.0 35.0 25.0 36.0 90-91 31.513912284112543 35.0 32.0 35.0 25.0 36.0 92-93 31.363351602894085 35.0 32.0 35.0 25.0 35.5 94-95 31.217918880737813 35.0 32.0 35.0 25.0 35.0 96-97 31.053780021669457 35.0 32.0 35.0 24.0 35.0 98-99 30.877718438266527 35.0 32.0 35.0 23.5 35.0 100-101 30.28856288718898 34.5 31.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48541335364037863 1101 2 0.490201451995933 1101 3 0.5221827546831612 1101 4 0.3937224099839298 1101 5 0.3792643203981001 1101 6 0.39912050688258205 1101 7 0.44430371265877255 1101 8 0.458767519095332 1101 9 0.5329389167419691 1101 10-11 0.5474339194981397 1101 12-13 0.5463923818676903 1101 14-15 0.6902969601232769 1101 16-17 0.6602018304417712 1101 18-19 0.7620980862841265 1101 20-21 0.7488584315577498 1101 22-23 0.7026207005817682 1101 24-25 0.6765011028633552 1101 26-27 0.6454353417191783 1101 28-29 0.7541005397601168 1101 30-31 0.7395618589953585 1101 32-33 0.6817331668654631 1101 34-35 0.775299372117324 1101 36-37 0.7376649565015967 1101 38-39 0.7064151297863219 1101 40-41 0.6037373799613022 1101 42-43 0.6635782771850032 1101 44-45 0.7426221886700972 1101 46-47 0.6220015536930035 1101 48-49 0.634843788784579 1101 50-51 0.5649665594058817 1101 52-53 0.5418758637388237 1101 54-55 0.4453224643661855 1101 56-57 0.4463904370530116 1101 58-59 0.4512612389394377 1101 60-61 0.42892486302878297 1101 62-63 0.38573722493916307 1101 64-65 0.42472349937122544 1101 66-67 0.44403028722077664 1101 68-69 0.3654183150266519 1101 70-71 0.3851080061467087 1101 72-73 0.45332523065382446 1101 74-75 0.41450273584639774 1101 76-77 0.3517320577139529 1101 78-79 0.34834043252865143 1101 80-81 0.3099968865964584 1101 82-83 0.3110272458711947 1101 84-85 0.288372634670246 1101 86-87 0.2887794863332296 1101 88-89 0.260693599243389 1101 90-91 0.2625242316865908 1101 92-93 0.27933962556897285 1101 94-95 0.29706842444893056 1101 96-97 0.35537300556782725 1101 98-99 0.32270987350217695 1101 100-101 0.35279469610150826 1102 1 0.3934931292395589 1102 2 0.3716496703806129 1102 3 0.39132054554523066 1102 4 0.3438994487232989 1102 5 0.32666898180415416 1102 6 0.3689507560132057 1102 7 0.3977758222322123 1102 8 0.43506323552451676 1102 9 0.49631688787713557 1102 10-11 0.5003503552219968 1102 12-13 0.5379665728464715 1102 14-15 0.6386355731015527 1102 16-17 0.6191673911291105 1102 18-19 0.6559944682256855 1102 20-21 0.6862951849492305 1102 22-23 0.649798690340802 1102 24-25 0.5879073838023743 1102 26-27 0.5703290749366801 1102 28-29 0.6727175403520533 1102 30-31 0.5956040617020193 1102 32-33 0.6167309344695511 1102 34-35 0.7318823135605328 1102 36-37 0.7634028249734968 1102 38-39 0.7409084547651048 1102 40-41 0.5655384280882814 1102 42-43 0.5869032775260834 1102 44-45 0.5790635836468141 1102 46-47 0.5279433928939596 1102 48-49 0.5769231130543133 1102 50-51 0.510050761477018 1102 52-53 0.5856575143435236 1102 54-55 0.5283203166654431 1102 56-57 0.47931431296203897 1102 58-59 0.4255848354564762 1102 60-61 0.44407101908580415 1102 62-63 0.37945296164697595 1102 64-65 0.3238946381807608 1102 66-67 0.3123767128047561 1102 68-69 0.26810890591679026 1102 70-71 0.26090471451076525 1102 72-73 0.32694898714718335 1102 74-75 0.32155465345336154 1102 76-77 0.3699225389343326 1102 78-79 0.2905398432759725 1102 80-81 0.2463554002760162 1102 82-83 0.28817765041548427 1102 84-85 0.2705446749190017 1102 86-87 0.3427728981486844 1102 88-89 0.32216722504777096 1102 90-91 0.2691142528073449 1102 92-93 0.3061323759224379 1102 94-95 0.2759688935144453 1102 96-97 0.2501221610482389 1102 98-99 0.20938573599680055 1102 100-101 0.14592749681201767 1103 1 0.2919280894361407 1103 2 0.3084547639246793 1103 3 0.3493883701333367 1103 4 0.31680583955761676 1103 5 0.30910880190663903 1103 6 0.3358784895496001 1103 7 0.37932563630231897 1103 8 0.48991839030115614 1103 9 0.4443804824038011 1103 10-11 0.4826156239573649 1103 12-13 0.5175283617015154 1103 14-15 0.6467304497653075 1103 16-17 0.5879814722234187 1103 18-19 0.6126549421819263 1103 20-21 0.6403266698473047 1103 22-23 0.6157180369390147 1103 24-25 0.5969054729147913 1103 26-27 0.5736637952547028 1103 28-29 0.6495077163816205 1103 30-31 0.6668389874002045 1103 32-33 0.6536323083860438 1103 34-35 0.6970466207983037 1103 36-37 0.7619165882914345 1103 38-39 0.7384757029632851 1103 40-41 0.6658531844651421 1103 42-43 0.6673177060645443 1103 44-45 0.701737245083919 1103 46-47 0.6532020980196975 1103 48-49 0.6383538606736181 1103 50-51 0.5653647683845762 1103 52-53 0.5054927430498353 1103 54-55 0.479300421416994 1103 56-57 0.4362396823356889 1103 58-59 0.472381837219892 1103 60-61 0.3970103934803859 1103 62-63 0.33661212121579354 1103 64-65 0.3125666773917217 1103 66-67 0.3471963588382465 1103 68-69 0.41930996071623383 1103 70-71 0.43897862192860515 1103 72-73 0.38666170509191744 1103 74-75 0.367136298649406 1103 76-77 0.3583253704286804 1103 78-79 0.34544322116765613 1103 80-81 0.3030888999784054 1103 82-83 0.3486731681467461 1103 84-85 0.3495688983613334 1103 86-87 0.3382895444183056 1103 88-89 0.31837380463501574 1103 90-91 0.34117809363264584 1103 92-93 0.4201825515968025 1103 94-95 0.37806176150370163 1103 96-97 0.3287181469196554 1103 98-99 0.3583210853023644 1103 100-101 0.43060815758064663 1104 1 0.29913046213068384 1104 2 0.28079854292176165 1104 3 0.2944229389455373 1104 4 0.2919571267023997 1104 5 0.2269966401104213 1104 6 0.3468271412780837 1104 7 0.3065969022758992 1104 8 0.3568671648081434 1104 9 0.39618761837918015 1104 10-11 0.349760783485614 1104 12-13 0.4272844262628084 1104 14-15 0.49986314929500963 1104 16-17 0.4972656473063708 1104 18-19 0.4701785482672989 1104 20-21 0.562324599171717 1104 22-23 0.4796839198628433 1104 24-25 0.4611427407538784 1104 26-27 0.45839061409805737 1104 28-29 0.566082369850406 1104 30-31 0.5312579593443019 1104 32-33 0.510226695267967 1104 34-35 0.5226026832190556 1104 36-37 0.5697818841199762 1104 38-39 0.6155641238803042 1104 40-41 0.4921989469004586 1104 42-43 0.4321670893549978 1104 44-45 0.37099314476257206 1104 46-47 0.4355134517798973 1104 48-49 0.4678869570525208 1104 50-51 0.3808399484479281 1104 52-53 0.3902183098755714 1104 54-55 0.3645461005931381 1104 56-57 0.21830624645807006 1104 58-59 0.2852452075665042 1104 60-61 0.2246684521220601 1104 62-63 0.14832353438625034 1104 64-65 0.16165249249658586 1104 66-67 0.1815002481501935 1104 68-69 0.2433973055039118 1104 70-71 0.23718427874613468 1104 72-73 0.26275681158106323 1104 74-75 0.24947069357251195 1104 76-77 0.25216707945696015 1104 78-79 0.283549932244604 1104 80-81 0.2583821982310113 1104 82-83 0.2571764679199475 1104 84-85 0.25185039755954364 1104 86-87 0.28452903882936553 1104 88-89 0.29183054651765516 1104 90-91 0.19148676645964002 1104 92-93 0.23893898711880013 1104 94-95 0.2019614248119339 1104 96-97 0.2576019106138503 1104 98-99 0.23744558977095664 1104 100-101 0.22177604163250564 1105 1 0.22006218396891697 1105 2 0.20600485112439415 1105 3 0.22648093590705543 1105 4 0.16849308751304193 1105 5 0.22043472604339343 1105 6 0.24016240926185617 1105 7 0.22180673685469543 1105 8 0.22050303769394475 1105 9 0.33597417384044803 1105 10-11 0.35684978407942225 1105 12-13 0.3404651376129024 1105 14-15 0.29479462282667157 1105 16-17 0.4195075464036506 1105 18-19 0.4375874916779168 1105 20-21 0.42714426933363825 1105 22-23 0.42813842951122183 1105 24-25 0.3598871473312357 1105 26-27 0.3751865647055297 1105 28-29 0.49221964268177487 1105 30-31 0.4702879660501651 1105 32-33 0.38914433763475387 1105 34-35 0.4770284835270502 1105 36-37 0.479844425263515 1105 38-39 0.44307884403952613 1105 40-41 0.3761423129160306 1105 42-43 0.4562505008044653 1105 44-45 0.40535553637375443 1105 46-47 0.44777267353107675 1105 48-49 0.45487693499499926 1105 50-51 0.37200848722587665 1105 52-53 0.36115873698004464 1105 54-55 0.4141308544376088 1105 56-57 0.22272620250952713 1105 58-59 0.2570692505802299 1105 60-61 0.15844231487565708 1105 62-63 0.1721579388802965 1105 64-65 0.1405706039896728 1105 66-67 0.24507381974154185 1105 68-69 0.22448339500382986 1105 70-71 0.30865838657527433 1105 72-73 0.2575021675114826 1105 74-75 0.22841467308636254 1105 76-77 0.2699581526706041 1105 78-79 0.19763443193494368 1105 80-81 0.1613125725534985 1105 82-83 0.2180457378147196 1105 84-85 0.1459166502189433 1105 86-87 0.198685209125383 1105 88-89 0.19835617267407457 1105 90-91 0.24865490073178265 1105 92-93 0.24168229237261585 1105 94-95 0.15197383683671006 1105 96-97 0.20481282590106886 1105 98-99 0.227377802341735 1105 100-101 0.23150457806350389 1106 1 0.178510872280647 1106 2 0.21318315332468174 1106 3 0.2449133934459944 1106 4 0.1684379442527657 1106 5 0.20386162014901998 1106 6 0.21941053955865897 1106 7 0.2001972782310304 1106 8 0.25275959447670715 1106 9 0.31758669988113297 1106 10-11 0.3490798268274844 1106 12-13 0.3421036300281699 1106 14-15 0.3521249917707294 1106 16-17 0.40733977430258506 1106 18-19 0.3452681126854671 1106 20-21 0.4255503312157174 1106 22-23 0.3131720621518639 1106 24-25 0.2779988529549442 1106 26-27 0.3131068637669898 1106 28-29 0.3952819083204844 1106 30-31 0.37523070922508595 1106 32-33 0.395391435507662 1106 34-35 0.4606178674803161 1106 36-37 0.4360987071103537 1106 38-39 0.43493993167561484 1106 40-41 0.3774567752604625 1106 42-43 0.39096804184433864 1106 44-45 0.38216584414377763 1106 46-47 0.3376743672383 1106 48-49 0.33920886021740415 1106 50-51 0.3318630034608461 1106 52-53 0.387148409879309 1106 54-55 0.3463603469238379 1106 56-57 0.33352865532441456 1106 58-59 0.3307662536500544 1106 60-61 0.33092628980062955 1106 62-63 0.29303085968246734 1106 64-65 0.1565395080820906 1106 66-67 0.22471419046191698 1106 68-69 0.2604766849315183 1106 70-71 0.3066971064060837 1106 72-73 0.2375631112859793 1106 74-75 0.1469417028126614 1106 76-77 0.18219922146899847 1106 78-79 0.2210975509608062 1106 80-81 0.21078880589287508 1106 82-83 0.24479600135175872 1106 84-85 0.1838145810718501 1106 86-87 0.13225369808882803 1106 88-89 0.1295162709641069 1106 90-91 0.0654035180363195 1106 92-93 0.14356347237073663 1106 94-95 0.08490179761123784 1106 96-97 0.11665561830685789 1106 98-99 0.033218675595556135 1106 100-101 0.09412690481379116 1107 1 0.13970602994255188 1107 2 0.08897472259160821 1107 3 0.15925786176908474 1107 4 0.13274490582071508 1107 5 0.12483347319479776 1107 6 0.13708552919029415 1107 7 0.13779220370452094 1107 8 -0.1409369927620432 1107 9 0.01822144771006151 1107 10-11 -0.032469422094841605 1107 12-13 -0.0013368705122474012 1107 14-15 -0.1370576083939099 1107 16-17 -0.09952574885970478 1107 18-19 0.05635632700259663 1107 20-21 0.08957059193284067 1107 22-23 0.08739013467382506 1107 24-25 -2.25824683333542E-4 1107 26-27 0.15925244574614794 1107 28-29 6.108131075279744E-4 1107 30-31 0.008204606094466271 1107 32-33 0.07718127750015213 1107 34-35 0.13291282531565685 1107 36-37 0.3324787750388154 1107 38-39 0.1827827434176328 1107 40-41 -0.0552631949854856 1107 42-43 0.052342603545504574 1107 44-45 -0.02703633022689189 1107 46-47 0.05571625541082881 1107 48-49 0.2389995882333409 1107 50-51 0.05956908638886915 1107 52-53 0.20460780439853465 1107 54-55 0.18403806217726526 1107 56-57 -0.01578394993014598 1107 58-59 0.14903475331701088 1107 60-61 -0.06275464671358577 1107 62-63 -0.02995873896776402 1107 64-65 -0.10454169581012707 1107 66-67 0.11900240257970296 1107 68-69 0.20109035435679345 1107 70-71 0.3203721282424752 1107 72-73 0.3583888254460206 1107 74-75 0.29271907056895685 1107 76-77 0.28812169520249853 1107 78-79 0.3389431275986894 1107 80-81 0.3354686435180767 1107 82-83 0.29491189918885397 1107 84-85 0.3308374087434274 1107 86-87 0.271110892787771 1107 88-89 0.2480174696093087 1107 90-91 0.23291249609309972 1107 92-93 0.28589705234535145 1107 94-95 0.22041554982629208 1107 96-97 0.21665039485300142 1107 98-99 0.18183964450304302 1107 100-101 0.1816956933809486 1108 1 -0.12908270224637164 1108 2 -0.06964407504872483 1108 3 0.020020044566262385 1108 4 -0.13665141641859435 1108 5 -0.04809232301605704 1108 6 0.09571275310037919 1108 7 0.05125328032913501 1108 8 0.1537367451989411 1108 9 0.17280354119659336 1108 10-11 0.17566448735050244 1108 12-13 0.22727941227076798 1108 14-15 0.26020176364973224 1108 16-17 0.2074551160845175 1108 18-19 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22-23 -1.5406491252259968 2215 24-25 -1.1057842472824504 2215 26-27 -0.9469815788804681 2215 28-29 -0.9094569782667534 2215 30-31 -1.299698722362308 2215 32-33 -1.145833966389695 2215 34-35 -1.3137922958957233 2215 36-37 -1.3521718227526804 2215 38-39 -1.1223471594851588 2215 40-41 -1.4001155474076512 2215 42-43 -1.579688007181268 2215 44-45 -1.1384533755499433 2215 46-47 -1.0353751428385678 2215 48-49 -0.973300351532913 2215 50-51 -0.8886451716512553 2215 52-53 -0.8123410594256768 2215 54-55 -0.7593187395865613 2215 56-57 -0.6960301618165516 2215 58-59 -0.5652499942178508 2215 60-61 -0.6002228638567573 2215 62-63 -0.5524714624421847 2215 64-65 -0.5776423311056504 2215 66-67 -0.6787081469008243 2215 68-69 -0.4980705446447473 2215 70-71 -0.426556351160265 2215 72-73 -0.46003128373926927 2215 74-75 -0.42229951097287 2215 76-77 -0.5242651598935097 2215 78-79 -0.5465363169903981 2215 80-81 -0.5483882997898775 2215 82-83 -0.5661177207289825 2215 84-85 -0.6504477964852349 2215 86-87 -0.6912822517236243 2215 88-89 -0.643238951535114 2215 90-91 -0.6785816365409865 2215 92-93 -0.5933101920280102 2215 94-95 -0.6416240184744311 2215 96-97 -0.6324228785152108 2215 98-99 -0.6603561337623916 2215 100-101 -0.6361687905098812 2216 1 -0.6248454568625306 2216 2 -0.6553915314961571 2216 3 -0.589124144467128 2216 4 -0.48803447854311344 2216 5 -0.45864221887303813 2216 6 -0.5501421116949388 2216 7 -0.8425620532731983 2216 8 -1.8087696226198275 2216 9 -1.1612356402814257 2216 10-11 -0.9520337663415503 2216 12-13 -1.000015596080729 2216 14-15 -1.7761469640483227 2216 16-17 -1.0686962893951062 2216 18-19 -1.1190954659094103 2216 20-21 -1.0545366120370545 2216 22-23 -0.8853151328370075 2216 24-25 -1.180230245171579 2216 26-27 -1.3995772418726844 2216 28-29 -2.0634893561068637 2216 30-31 -1.2782696505029918 2216 32-33 -1.1535295892958075 2216 34-35 -2.502275211797439 2216 36-37 -2.385808024337557 2216 38-39 -2.5105788002358835 2216 40-41 -1.5452670688542227 2216 42-43 -1.3921332121058825 2216 44-45 -1.2050991819646626 2216 46-47 -1.5859960421022876 2216 48-49 -1.627357525425495 2216 50-51 -1.0772617800268236 2216 52-53 -0.8509207766722682 2216 54-55 -0.8660505852483951 2216 56-57 -0.9132201855754403 2216 58-59 -0.9671095352220505 2216 60-61 -1.1333242955462026 2216 62-63 -0.9934333853287001 2216 64-65 -0.9812955126403011 2216 66-67 -0.965433200888242 2216 68-69 -1.1047053572942787 2216 70-71 -1.189548897120666 2216 72-73 -1.016020350156687 2216 74-75 -0.8609748890477462 2216 76-77 -0.9633414626422123 2216 78-79 -0.9536274813829806 2216 80-81 -0.9030399883170404 2216 82-83 -1.3067676486855078 2216 84-85 -0.9565054975612384 2216 86-87 -1.0750496992724585 2216 88-89 -0.8919425558679137 2216 90-91 -1.0609565718968632 2216 92-93 -1.281163418032623 2216 94-95 -1.0503707266966344 2216 96-97 -0.9380607523825297 2216 98-99 -0.9402368917354522 2216 100-101 -0.9906019861003834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 49.0 11 864.0 12 3918.0 13 7932.0 14 11425.0 15 14110.0 16 16012.0 17 18346.0 18 23290.0 19 33954.0 20 32740.0 21 32097.0 22 36763.0 23 43350.0 24 51104.0 25 59590.0 26 69417.0 27 82776.0 28 97570.0 29 113676.0 30 133195.0 31 158674.0 32 193725.0 33 243228.0 34 319528.0 35 448014.0 36 725904.0 37 1213143.0 38 1214265.0 39 238550.0 40 219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.187224476418642 40.204223449158434 12.279097331928618 20.329454742494306 2 23.950328913976946 27.5081154829857 31.57698404304028 16.964571559997076 3 22.442942423831536 27.78605813771057 23.60198378414066 26.169015654317235 4 25.16830942630407 26.117632984902517 25.519704329888782 23.194353258904634 5 22.979903875213466 29.21720748320265 24.503497536010414 23.299391105573466 6 22.517088305650162 25.106321222711358 26.330547718185375 26.0460427534531 7 25.89639405552053 26.015409970616837 24.435078042539494 23.653117931323134 8 25.721266474793747 24.881362679730064 26.17791526521555 23.219455580260636 9 21.993123612136532 25.735898029930134 26.115355291274383 26.155623066658958 10-11 25.066504676680353 25.402076902493185 25.194560492625527 24.336857928200935 12-13 24.619742329546035 25.504033822717215 24.982330046265165 24.893893801471584 14-15 24.492709250394114 25.52161485833146 25.10769689795682 24.877978993317605 16-17 24.574608644565032 26.99981099531852 25.03434856312539 23.391231796991054 18-19 23.01341174627832 25.387219793326082 26.629837204089984 24.96953125630562 20-21 22.947794323610374 27.452200780228164 25.24090090080501 24.359103995356453 22-23 22.36917938187275 27.03638083425685 27.322825027581267 23.271614756289136 24-25 22.407337720253054 25.497893033037094 28.771999579156613 23.322769667553242 26-27 22.3177877516242 25.43308957736794 29.572198390467307 22.676924280540554 28-29 22.374415775669213 25.383946725819982 29.698142963453883 22.54349453505692 30-31 22.41195302269086 25.335648820533923 29.61106644416467 22.64133171261055 32-33 22.36971301605886 25.33366151771974 29.717956564352967 22.578668901868433 34-35 22.42076973775296 25.433853663563973 29.658459504522778 22.48691709416029 36-37 22.450125211383458 25.37709319072522 29.511853882386625 22.660927715504695 38-39 22.509800902223198 25.433515346815383 29.335408486312442 22.721275264648973 40-41 23.007481385231472 25.954091701294494 27.8523324449096 23.186094468564434 42-43 23.035967192828345 26.08783382978814 27.730161438872198 23.146037538511315 44-45 23.084533119873697 26.351538749797665 27.443508957100377 23.12041917322826 46-47 23.027087884758796 26.704616005738785 27.271452513451173 22.99684359605125 48-49 23.038289801661325 26.74502450408236 27.07218611040354 23.144499583852777 50-51 22.974496512858003 26.773947287217194 27.084083002414584 23.167473197510223 52-53 23.07448258587854 26.87378132370597 26.958432317521407 23.093303772894085 54-55 23.087443023240034 26.687594621267912 26.940819738041945 23.284142617450108 56-57 23.045793223434515 26.662557818920256 27.026784200170717 23.264864757474506 58-59 23.11328758388431 26.753218623548214 26.944919984882443 23.18857380768503 60-61 23.159657205378057 26.703649252815293 26.809672779856346 23.32702076195031 62-63 23.150929821187958 26.709706979849678 26.90810419219545 23.231259006766916 64-65 23.18871905763314 26.88972281918899 26.75178432209065 23.169773801087224 66-67 23.27856292923065 26.748284496503004 26.65130737295766 23.321845201308687 68-69 23.264900234646014 26.758142188246133 26.666265183342475 23.310692393765382 70-71 23.32767319555946 26.892930267664354 26.578315892451766 23.201080644324424 72-73 23.372075490441297 26.792179547901736 26.471829449361127 23.363915512295847 74-75 23.356518163480462 26.794790216284326 26.575974475103877 23.272717145131335 76-77 23.40503847675988 26.948668147635953 26.443497142869305 23.202796232734862 78-79 23.46619191073652 26.847887767158863 26.34823570982699 23.33768461227763 80-81 23.435108728555694 26.85881851350928 26.413246828371662 23.292825929563364 82-83 23.518863965095733 26.967853048997032 26.32069751773968 23.192585468167557 84-85 23.561737728623765 26.915847084876294 26.255696746814326 23.266718439685615 86-87 23.573028338455053 26.885363325261096 26.341045952161164 23.200562384122687 88-89 23.606731651384283 26.968645985367797 26.256220035094017 23.168402328153903 90-91 23.67663054854093 26.8893722456411 26.20940790729389 23.22458929852408 92-93 23.65276328141131 26.831304985181188 26.362420947992593 23.15351078541491 94-95 23.731061398921636 27.00389255525747 26.21832154663439 23.046724499186507 96-97 23.83964815160389 26.89436565753035 26.130879188168787 23.135107002696977 98-99 23.846202559039334 26.87614812996281 26.24649042080892 23.03115889018893 100-101 23.928030655114355 27.003661953642688 26.047960169070006 23.020347222172948 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1631.0 1 1190.5 2 620.5 3 387.0 4 239.0 5 167.0 6 120.5 7 108.5 8 102.5 9 86.0 10 87.0 11 97.5 12 116.5 13 166.0 14 210.5 15 252.0 16 1278.0 17 1604.5 18 1141.0 19 1622.5 20 2420.5 21 3691.5 22 5330.5 23 7357.5 24 10031.5 25 13686.0 26 18316.0 27 23948.5 28 30584.0 29 38291.5 30 47537.5 31 57696.5 32 67704.5 33 78257.5 34 88802.0 35 100747.5 36 114573.5 37 129095.0 38 144501.0 39 160543.0 40 175361.0 41 186184.5 42 197506.0 43 209901.5 44 220109.5 45 229242.5 46 236972.0 47 241571.0 48 239600.5 49 234938.5 50 230845.5 51 227106.0 52 224242.5 53 217701.0 54 204664.5 55 189024.5 56 170877.0 57 149539.5 58 131140.5 59 114183.0 60 94930.5 61 76706.0 62 61427.0 63 48258.0 64 37740.0 65 29422.0 66 22599.5 67 17122.5 68 13226.5 69 10872.0 70 9209.0 71 7736.5 72 6627.0 73 5639.5 74 4399.0 75 2964.5 76 1719.0 77 1035.5 78 699.5 79 418.0 80 189.5 81 88.0 82 48.0 83 22.5 84 16.0 85 14.5 86 9.0 87 4.5 88 4.0 89 3.5 90 1.5 91 2.0 92 2.0 93 1.0 94 3.0 95 3.0 96 0.5 97 1.0 98 1.0 99 0.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0881607711885633 2 0.04470123609560956 3 0.052222396454553385 4 0.05873245742562033 5 0.04365465953622822 6 0.017224166765411462 7 0.0214104730029368 8 0.012612134469832696 9 0.0029446052348695187 10-11 7.538898944696057E-4 12-13 5.942426226995715E-4 14-15 0.001401348274425855 16-17 2.660787862833902E-5 18-19 0.0016585577678331323 20-21 0.006368152285049139 22-23 0.01600907364138398 24-25 0.019991386142758718 26-27 0.010190817514653845 28-29 0.017765193630854357 30-31 4.5233393668176337E-4 32-33 0.006261720770535784 34-35 2.572094934072772E-4 36-37 0.0028559123061083886 38-39 0.010190817514653845 40-41 0.0018270743324792797 42-43 0.002199584633276026 44-45 0.002492271298187755 46-47 0.0 48-49 0.0 50-51 0.0038758809868613847 52-53 0.0029091280633650666 54-55 0.0026873957414622414 56-57 0.0 58-59 0.004869241788986041 60-61 0.0 62-63 0.0 64-65 0.0023592319050460596 66-67 2.8381737203561625E-4 68-69 0.0 70-71 8.957985804874137E-4 72-73 0.00280269654885171 74-75 0.004700725224339894 76-77 0.002288277562037156 78-79 8.869292876113008E-5 80-81 0.001126400195266352 82-83 0.001170746659646917 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5637428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.72149118039601 #Duplication Level Percentage of deduplicated Percentage of total 1 81.4639447244492 54.353958694526064 2 10.900174869714622 14.545518428688768 3 3.3518867506473606 6.709286468130124 4 1.414637952423146 3.775470146642177 5 0.7860859562473673 2.6224413598395944 6 0.48318581272302025 1.9343326765254885 7 0.31303771532451724 1.4620440213509265 8 0.22893733172044153 1.222003212739907 9 0.16587228632404344 0.9960521660137609 >10 0.8575972482540134 10.116950235810776 >50 0.02984338586551433 1.317229701597096 >100 0.0043959731646313754 0.40303576987381695 >500 1.5999914653555742E-4 0.0743714860265123 >1k 2.1332799612044305E-4 0.22576208531827688 >5k 0.0 0.0 >10k+ 2.6665999468517138E-5 0.24154354691668986 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 13576 0.2408190401722204 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7738585752226015E-5 0.0 0.0 0.0 0.0 2 1.7738585752226015E-5 0.0 0.0 0.0 0.0 3 3.547717150445203E-5 0.0 0.0 0.0 0.0 4 5.321575725667804E-5 0.0 0.0 0.0 0.0 5 5.321575725667804E-5 0.0 0.0 0.0 0.0 6 1.5964727177003414E-4 0.0 0.0 0.0 0.0 7 2.306016147789382E-4 0.0 0.0 0.0 0.0 8 2.8381737203561625E-4 0.0 0.0 0.0 0.0 9 5.676347440712325E-4 0.0 0.0 0.0 0.0 10-11 8.514521161068487E-4 0.0 0.0 0.0 0.0 12-13 0.00117961595252303 0.0 0.0 0.0 0.0 14-15 0.0013392632242930642 0.0 0.0 0.0 0.0 16-17 0.0015432569604436632 0.0 2.660787862833902E-5 0.0 0.0 18-19 0.0017029042322136974 0.0 3.547717150445203E-5 0.0 0.0 20-21 0.0019867216042493137 0.0 1.5964727177003414E-4 0.0 0.0 22-23 0.002376970490798286 0.0 4.2572605805342434E-4 0.0 0.0 24-25 0.0028115658417278235 0.0 5.853733298234585E-4 0.0 0.0 26-27 0.0031840761425245695 0.0 7.272820158412667E-4 0.0 0.0 28-29 0.003592063614825768 0.0 9.756222163724308E-4 0.0 0.0 30-31 0.003911358158365836 0.0 0.001064315145133561 0.0 0.0 32-33 0.004328214923543148 0.0 0.001117530902390239 0.0 0.0 34-35 0.004771679567348798 0.0 0.001117530902390239 0.0 0.0 36-37 0.005410268654428935 0.0 0.001117530902390239 0.0 0.0 38-39 0.0060311191557568455 0.0 0.001117530902390239 0.0 0.0 40-41 0.0067938783431025634 0.0 0.001117530902390239 0.0 0.0 42-43 0.00769854621646609 0.0 0.001117530902390239 0.0 0.0 44-45 0.008922508633369686 0.0 0.001117530902390239 0.0 0.0 46-47 0.010040039535759925 0.0 0.001117530902390239 0.0 0.0 48-49 0.011760682353725849 0.0 0.001117530902390239 0.0 0.0 50-51 0.013756273250851273 0.0 0.001117530902390239 0.0 0.0 52-53 0.03659470240684227 0.0 0.001117530902390239 8.869292876113008E-6 0.0 54-55 0.06949977897722152 0.0 0.001117530902390239 1.7738585752226015E-5 0.0 56-57 0.10709671147906458 0.0 0.001117530902390239 1.7738585752226015E-5 0.0 58-59 0.15108840414458508 0.0 0.001117530902390239 1.7738585752226015E-5 0.0 60-61 0.20163450424555313 0.0 0.001117530902390239 1.7738585752226015E-5 0.0 62-63 0.25835363218829577 0.0 0.001117530902390239 1.7738585752226015E-5 0.0 64-65 0.32284226069051347 0.0 0.001117530902390239 1.7738585752226015E-5 0.0 66-67 0.39307819097645236 0.0 0.001117530902390239 2.660787862833902E-5 0.0 68-69 0.4725470551464249 0.0 0.001117530902390239 3.547717150445203E-5 0.0 70-71 0.563590346519725 0.0 0.001117530902390239 3.547717150445203E-5 8.869292876113008E-6 72-73 0.662092713201836 8.869292876113008E-6 0.001117530902390239 3.547717150445203E-5 1.7738585752226015E-5 74-75 0.7705375571980697 1.7738585752226015E-5 0.001117530902390239 3.547717150445203E-5 1.7738585752226015E-5 76-77 0.888197596492585 1.7738585752226015E-5 0.001117530902390239 3.547717150445203E-5 1.7738585752226015E-5 78-79 1.0176005795550735 1.7738585752226015E-5 0.001117530902390239 5.321575725667804E-5 1.7738585752226015E-5 80-81 1.1595713506230148 1.7738585752226015E-5 0.001117530902390239 5.321575725667804E-5 3.547717150445203E-5 82-83 1.3145001585829568 1.7738585752226015E-5 0.001117530902390239 5.321575725667804E-5 3.547717150445203E-5 84-85 1.4812517339467572 1.7738585752226015E-5 0.001117530902390239 5.321575725667804E-5 3.547717150445203E-5 86-87 1.662389302355613 1.7738585752226015E-5 0.001117530902390239 5.321575725667804E-5 3.547717150445203E-5 88-89 1.8583918056248345 1.7738585752226015E-5 0.001117530902390239 7.095434300890406E-5 3.547717150445203E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 17900 0.0 88.909386 1 TCGACGG 19190 0.0 83.02044 2 TATCTCG 7745 0.0 80.636185 2 CGACGGC 19895 0.0 80.0279 3 CGGCGCG 20355 0.0 78.0213 6 ACGGCGC 20455 0.0 77.70217 5 GACGGCG 20590 0.0 77.30217 4 ATCTCGA 8095 0.0 77.029594 3 ATATCTC 8500 0.0 73.89735 1 TCTCGAG 7000 0.0 69.79558 4 AGGGCGC 7000 0.0 69.67944 9 TCGAGGG 7130 0.0 68.36057 6 CTCGAGG 7240 0.0 67.46694 5 CGAGGGC 7535 0.0 64.81062 7 GAGGGCG 7890 0.0 62.191734 8 CGCGCCG 26405 0.0 60.41658 9 GGCGCGC 26595 0.0 59.99932 7 GCGCGCC 26595 0.0 59.995598 8 CTCGACG 2285 0.0 58.848682 5 TCTCGAC 2510 0.0 53.77464 4 >>END_MODULE