FastQCFastQC Report
Tue 31 May 2016
SRR2048238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5637428
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT200490.35564090574637935No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT144330.25602100816187806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG40600.066.831411
TCGACGG42900.064.023752
TATCTCG37100.055.716772
CGACGGC51300.053.3550763
ACGGCGC56950.048.1238145
ATATCTC43600.047.9553221
ATCTCGA74700.046.8196141
GACGGCG59050.046.5134474
CGGCGCG58800.046.3673746
TCTCGAG79750.042.3652232
TCGAGGG88100.038.2420274
CTCGAGG89750.037.6448673
GGGCGCG90050.037.2918248
CGAGGGC93050.036.1405565
AGGGCGC94350.035.692947
GAGGGCG100050.033.659466
CGCGCCG146800.023.5873649
GGCGCGC147750.023.5642979
GCGCGCC149250.023.3274698
GGTCGAC6550.023.2155651
Produced by FastQC (version 0.11.5)