FastQCFastQC Report
Tue 31 May 2016
SRR2048237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779221
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT89850.1880013500108072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG152050.089.638771
TCGACGG158650.085.905492
CGACGGC166800.081.5932543
CGGCGCG169050.080.541576
ACGGCGC171300.079.554965
GACGGCG173200.078.686364
TATCTCG41800.074.464092
GGCGCGC205700.066.443977
CGCGCCG205600.066.4113169
GCGCGCC206600.066.142758
ATCTCGA47700.065.3525853
ATATCTC51300.061.625281
AGGGCGC42850.061.402539
TCGAGGG44450.059.2102786
CTCGAGG44850.058.6938745
TCTCGAG44750.058.6157384
CGAGGGC47650.055.2327777
GAGGGCG51300.051.386428
CTCGACG13050.037.1394235
ATCCATA190850.035.97934316-17
Produced by FastQC (version 0.11.5)