FastQCFastQC Report
Tue 31 May 2016
SRR2048237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779221
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT226930.4748263367607398No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT115280.24121085842232448No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.11585570116970946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG31850.069.6747361
TCGACGG34150.065.538742
TATCTCG30850.058.0702972
ATATCTC34500.052.4775731
CGACGGC43300.051.689333
ATCTCGA69000.049.034161
ACGGCGC49150.045.7103585
TCTCGAG72450.045.2562562
GACGGCG50550.044.2759254
CGGCGCG50400.044.1996966
CTCGAGG82500.039.685623
TCGAGGG83250.039.2710084
GGGCGCG84000.038.7901848
AGGGCGC88450.037.0534177
CGAGGGC90250.036.367035
GAGGGCG94500.034.8320056
GGCGCGC132900.025.4109949
CGCGCCG132350.023.5427369
GCGCGCC133300.023.339328
TCCATAT67050.018.98510718-19
Produced by FastQC (version 0.11.5)