##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048237_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4779221 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29817704600813 33.0 31.0 34.0 30.0 34.0 2 32.43666572439316 34.0 31.0 34.0 31.0 34.0 3 32.509809443840325 34.0 31.0 34.0 31.0 34.0 4 36.06622104313653 37.0 35.0 37.0 35.0 37.0 5 35.932369731385094 37.0 35.0 37.0 35.0 37.0 6 35.92239195467211 37.0 35.0 37.0 35.0 37.0 7 35.90175511866892 37.0 35.0 37.0 35.0 37.0 8 35.87443685906134 37.0 35.0 37.0 35.0 37.0 9 37.4805573962786 39.0 37.0 39.0 34.0 39.0 10-11 37.47838821431359 39.0 37.0 39.0 34.0 39.0 12-13 37.475858617963056 39.0 37.0 39.0 34.5 39.0 14-15 38.81750550978914 40.0 38.0 41.0 35.0 41.0 16-17 38.82332392663993 40.0 38.0 41.0 35.0 41.0 18-19 38.85639437891656 40.0 38.0 41.0 35.0 41.0 20-21 38.87764292548932 40.0 38.0 41.0 35.0 41.0 22-23 38.855342010758655 40.0 38.0 41.0 35.0 41.0 24-25 38.838361000673544 40.0 38.0 41.0 35.0 41.0 26-27 38.751814364725966 40.0 38.0 41.0 34.5 41.0 28-29 38.66791156968887 40.0 38.0 41.0 34.0 41.0 30-31 38.54993721780181 40.0 38.0 41.0 34.0 41.0 32-33 38.4604881213905 40.0 38.0 41.0 34.0 41.0 34-35 38.41316252167456 40.0 38.0 41.0 34.0 41.0 36-37 38.34046688780452 40.0 38.0 41.0 34.0 41.0 38-39 38.25300388912754 40.0 38.0 41.0 33.5 41.0 40-41 38.132220292805044 40.0 37.5 41.0 33.0 41.0 42-43 38.0567279688468 40.0 37.0 41.0 33.0 41.0 44-45 37.95085276449865 40.0 37.0 41.0 33.0 41.0 46-47 37.83726950480005 40.0 37.0 41.0 33.0 41.0 48-49 37.73189145260284 40.0 37.0 41.0 33.0 41.0 50-51 37.646968512232434 40.0 36.0 41.0 33.0 41.0 52-53 37.61689771199114 40.0 36.0 41.0 33.0 41.0 54-55 37.43649958853126 39.0 36.0 41.0 32.0 41.0 56-57 37.202490008308885 39.0 35.5 41.0 32.0 41.0 58-59 36.96250822048196 39.0 35.0 41.0 31.5 41.0 60-61 36.7077717268149 39.0 35.0 40.5 31.0 41.0 62-63 36.412622057025615 38.0 35.0 40.0 31.0 41.0 64-65 36.11024620958102 37.5 35.0 40.0 31.0 41.0 66-67 35.781909227466144 37.0 35.0 40.0 30.5 41.0 68-69 35.43525587119741 36.5 34.5 39.0 30.0 41.0 70-71 35.08306322306501 36.0 34.0 39.0 30.0 41.0 72-73 34.717790409775986 35.5 34.0 38.5 30.0 40.0 74-75 34.38187593752203 35.0 34.0 37.0 29.5 39.5 76-77 34.134533849763386 35.0 34.0 37.0 29.5 39.0 78-79 33.98033403351718 35.0 34.0 36.5 30.0 39.0 80-81 33.73434216580485 35.0 34.0 36.0 30.0 38.0 82-83 33.501201555651015 35.0 34.0 36.0 30.0 37.0 84-85 33.300498867911735 35.0 34.0 35.0 30.0 37.0 86-87 33.10603349374301 35.0 34.0 35.0 29.0 36.0 88-89 32.971084618183596 35.0 34.0 35.0 29.0 36.0 90-91 32.84481717836442 35.0 34.0 35.0 29.0 36.0 92-93 32.71924472628489 35.0 33.5 35.0 29.0 36.0 94-95 32.61316268906585 35.0 33.0 35.0 29.0 35.0 96-97 32.50807453767047 35.0 33.0 35.0 29.0 35.0 98-99 32.37233787682135 35.0 33.0 35.0 29.0 35.0 100-101 31.92848489324934 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31714036117590894 1101 2 0.344649069324241 1101 3 0.30309588696849943 1101 4 0.20762739666685093 1101 5 0.22356513768510666 1101 6 0.29767770764014756 1101 7 0.275341675356529 1101 8 0.30228383313153273 1101 9 0.3984500403175062 1101 10-11 0.3799069923787499 1101 12-13 0.3835894211294857 1101 14-15 0.4601163472206693 1101 16-17 0.4712908254610966 1101 18-19 0.5429588213076357 1101 20-21 0.4883057457718536 1101 22-23 0.5237263504018728 1101 24-25 0.5097922928872336 1101 26-27 0.5310305244860842 1101 28-29 0.5033009157881594 1101 30-31 0.5639934195023102 1101 32-33 0.5182030749977073 1101 34-35 0.5571653563333072 1101 36-37 0.5695311130959411 1101 38-39 0.5767630814776012 1101 40-41 0.5591257288788753 1101 42-43 0.5165058285078814 1101 44-45 0.5226851644618051 1101 46-47 0.5305920190035778 1101 48-49 0.5931680524018574 1101 50-51 0.6058439410956211 1101 52-53 0.5812063538413028 1101 54-55 0.5955665540626995 1101 56-57 0.5797542777843105 1101 58-59 0.5559089737950274 1101 60-61 0.5675289240306753 1101 62-63 0.6212337422742493 1101 64-65 0.5665738664346165 1101 66-67 0.517324983141819 1101 68-69 0.46050686576782596 1101 70-71 0.426717506531034 1101 72-73 0.40795467710964317 1101 74-75 0.34141647675071596 1101 76-77 0.38716588155848797 1101 78-79 0.39244355858294 1101 80-81 0.40406727737423864 1101 82-83 0.4234977907515969 1101 84-85 0.3974882814524463 1101 86-87 0.3800542615517486 1101 88-89 0.36259265706063104 1101 90-91 0.3326168629826327 1101 92-93 0.42420973270552054 1101 94-95 0.4049194660400559 1101 96-97 0.47967467828328125 1101 98-99 0.48939880273445624 1101 100-101 0.47502569903332414 1102 1 0.3784734187814678 1102 2 0.3770710200551335 1102 3 0.3505165129136216 1102 4 0.22869795281837924 1102 5 0.23495708363385148 1102 6 0.2456007828233595 1102 7 0.24975489219487912 1102 8 0.2544053482987181 1102 9 0.35002912033456113 1102 10-11 0.38856212479270624 1102 12-13 0.36818579767884074 1102 14-15 0.5135562261804907 1102 16-17 0.4852865005202247 1102 18-19 0.47815801577326766 1102 20-21 0.46998755323200925 1102 22-23 0.5267416920673469 1102 24-25 0.49680632269167546 1102 26-27 0.4996174708225638 1102 28-29 0.5070817655251432 1102 30-31 0.5612692479780961 1102 32-33 0.5730304651051128 1102 34-35 0.522383509775814 1102 36-37 0.5855989873640794 1102 38-39 0.5836077406522264 1102 40-41 0.6226370897246696 1102 42-43 0.5789363176446329 1102 44-45 0.5731847056335653 1102 46-47 0.5575852157439556 1102 48-49 0.5967225980326489 1102 50-51 0.5691763346927061 1102 52-53 0.5613078292002882 1102 54-55 0.5493718800246228 1102 56-57 0.6134579587500468 1102 58-59 0.5148283363468877 1102 60-61 0.5211303622310268 1102 62-63 0.5030401522986736 1102 64-65 0.5389747013727728 1102 66-67 0.5375196054809379 1102 68-69 0.5601988126859112 1102 70-71 0.5084225381320167 1102 72-73 0.4674375986823236 1102 74-75 0.4568337519571841 1102 76-77 0.4681309718377946 1102 78-79 0.43955189051609267 1102 80-81 0.4282540689369654 1102 82-83 0.38590016858049836 1102 84-85 0.36846343687243177 1102 86-87 0.4052164670292555 1102 88-89 0.379436136852803 1102 90-91 0.3690893462127818 1102 92-93 0.3981608901159177 1102 94-95 0.39659686293293817 1102 96-97 0.3976680155802015 1102 98-99 0.4723910171946528 1102 100-101 0.4729139582001949 1103 1 0.3032719812058957 1103 2 0.30177341047701134 1103 3 0.27645807519142807 1103 4 0.1903541354310221 1103 5 0.23883683649338394 1103 6 0.2616237259616412 1103 7 0.2423412219562806 1103 8 0.21929732307687289 1103 9 0.3130555821050365 1103 10-11 0.3183907227807836 1103 12-13 0.3651283993817316 1103 14-15 0.4751043971616795 1103 16-17 0.4322067100552758 1103 18-19 0.4562419832848761 1103 20-21 0.43001694205293717 1103 22-23 0.40776872271560194 1103 24-25 0.4336591142939099 1103 26-27 0.4281673888884967 1103 28-29 0.42517598019160374 1103 30-31 0.42889874321978283 1103 32-33 0.39143803421744394 1103 34-35 0.3966401913316844 1103 36-37 0.4359759528344114 1103 38-39 0.44270066359199234 1103 40-41 0.43793989402990974 1103 42-43 0.34916946799800996 1103 44-45 0.4231542791463099 1103 46-47 0.4460601644162523 1103 48-49 0.38842103556937246 1103 50-51 0.4582647216396012 1103 52-53 0.4563558664360414 1103 54-55 0.4954266214802203 1103 56-57 0.33310151796793974 1103 58-59 0.3042365451669298 1103 60-61 0.35330279903131867 1103 62-63 0.34971748555697246 1103 64-65 0.33433929878781754 1103 66-67 0.3328130292186202 1103 68-69 0.27570979779900284 1103 70-71 0.2793224970250776 1103 72-73 0.30346464611869806 1103 74-75 0.2783240294713707 1103 76-77 0.26347005129505163 1103 78-79 0.2742673932670243 1103 80-81 0.22819522254371805 1103 82-83 0.2283707002158124 1103 84-85 0.2336869132656858 1103 86-87 0.2435948279929221 1103 88-89 0.21526599570940164 1103 90-91 0.25877284039506065 1103 92-93 0.30073480625425475 1103 94-95 0.2744841535030247 1103 96-97 0.30816772786362634 1103 98-99 0.3533859265539405 1103 100-101 0.3449366033091934 1104 1 0.25321281717844357 1104 2 0.25153347552107874 1104 3 0.21556620201269538 1104 4 0.13694195705638634 1104 5 0.16660881131056016 1104 6 0.22997237098088874 1104 7 0.20282285646011644 1104 8 0.2260689608711388 1104 9 0.29162386605478474 1104 10-11 0.2416790444317911 1104 12-13 0.26199493258209117 1104 14-15 0.3619156359522222 1104 16-17 0.32611082668361036 1104 18-19 0.3819604801536869 1104 20-21 0.35224514099208903 1104 22-23 0.38332437773414085 1104 24-25 0.39764448066522107 1104 26-27 0.3703081266179282 1104 28-29 0.3687948532943395 1104 30-31 0.4100306729838721 1104 32-33 0.4399227085559474 1104 34-35 0.35480951532242244 1104 36-37 0.40342014793495906 1104 38-39 0.3879667787989973 1104 40-41 0.407202416857146 1104 42-43 0.41838711664638595 1104 44-45 0.424455987499158 1104 46-47 0.4458914598989807 1104 48-49 0.39143556294928317 1104 50-51 0.4480103153562638 1104 52-53 0.4670089791228662 1104 54-55 0.43758508878986646 1104 56-57 0.4341932353709126 1104 58-59 0.39723173282472146 1104 60-61 0.44727563950248594 1104 62-63 0.4376281288588544 1104 64-65 0.39101047003348555 1104 66-67 0.3966013237443349 1104 68-69 0.40286871204852304 1104 70-71 0.31998302074079277 1104 72-73 0.2950765597015703 1104 74-75 0.30977862150665914 1104 76-77 0.35287699394124417 1104 78-79 0.3095814671493713 1104 80-81 0.27524825741346604 1104 82-83 0.27323825059855267 1104 84-85 0.2995265942284888 1104 86-87 0.29009512469792753 1104 88-89 0.3222649292960682 1104 90-91 0.2839312595034116 1104 92-93 0.3596187803419042 1104 94-95 0.35176848763951796 1104 96-97 0.2749423175847383 1104 98-99 0.289864219273241 1104 100-101 0.32497348212310584 1105 1 0.18652758886708654 1105 2 0.21455215428221663 1105 3 0.15618949778147595 1105 4 0.14765827657275565 1105 5 0.15247096122398318 1105 6 0.1776971592119665 1105 7 0.19465183513565165 1105 8 0.16977684823394412 1105 9 0.26262229534174963 1105 10-11 0.2062774200562174 1105 12-13 0.21395221717179425 1105 14-15 0.2956842681802456 1105 16-17 0.2332283994634352 1105 18-19 0.26881684461128685 1105 20-21 0.2863671235404297 1105 22-23 0.31042320255014033 1105 24-25 0.2665025742345577 1105 26-27 0.2901363801310879 1105 28-29 0.17484097692825884 1105 30-31 0.2016439046543752 1105 32-33 0.21793739177648774 1105 34-35 0.26890840637668845 1105 36-37 0.2878833958620035 1105 38-39 0.3079595080712352 1105 40-41 0.33812432728451114 1105 42-43 0.2095816078892767 1105 44-45 0.264712953740073 1105 46-47 0.21132422515002247 1105 48-49 0.2556159211345701 1105 50-51 0.3170388711609391 1105 52-53 0.275832119575945 1105 54-55 0.22754485077614106 1105 56-57 0.23112538120694381 1105 58-59 0.1904471332893536 1105 60-61 0.19285162531043198 1105 62-63 0.03506916305581598 1105 64-65 0.0695891402531501 1105 66-67 0.16393050915504404 1105 68-69 0.11382392572916444 1105 70-71 0.19386692993910515 1105 72-73 0.08702201198389048 1105 74-75 0.06304915618247975 1105 76-77 0.11994987252921874 1105 78-79 0.09562943333340712 1105 80-81 0.1993921921314552 1105 82-83 0.13173036938196248 1105 84-85 0.17467411928415544 1105 86-87 0.22692849515784985 1105 88-89 0.17156984093715977 1105 90-91 0.18480947474758835 1105 92-93 0.10344998417502183 1105 94-95 0.04465070240977553 1105 96-97 0.12195921776130803 1105 98-99 0.13760663590649358 1105 100-101 0.09052897425476658 1106 1 0.09438305305885564 1106 2 0.12955064764777546 1106 3 0.06646273460303576 1106 4 0.07037851732746958 1106 5 0.10080330972250806 1106 6 0.1047215361092384 1106 7 0.09045832326937386 1106 8 0.12993688456574404 1106 9 0.1957857137451171 1106 10-11 0.1629192099944703 1106 12-13 0.11996949477849483 1106 14-15 0.19008743489457913 1106 16-17 0.22126720641261244 1106 18-19 0.17906045125858583 1106 20-21 0.18880305116068286 1106 22-23 0.2353838316110597 1106 24-25 0.19366440703927168 1106 26-27 0.15420528194822936 1106 28-29 0.1950879403395689 1106 30-31 0.176106107899912 1106 32-33 0.2299598949061732 1106 34-35 0.15608929043298048 1106 36-37 0.2076073689276825 1106 38-39 0.2477857071390943 1106 40-41 0.24811078369138784 1106 42-43 0.20190957472556192 1106 44-45 0.2285663809627394 1106 46-47 0.138932676009631 1106 48-49 0.18014177594525194 1106 50-51 0.21486574045755447 1106 52-53 0.10069016789910279 1106 54-55 0.13683386164454703 1106 56-57 0.16719520976666757 1106 58-59 0.17256876551010691 1106 60-61 0.05534750787331433 1106 62-63 0.09190880486676889 1106 64-65 0.013384650361849992 1106 66-67 0.03843389379704121 1106 68-69 0.06566796644321471 1106 70-71 0.036128445014142585 1106 72-73 0.052890059635430475 1106 74-75 0.03499679489562624 1106 76-77 0.09589267533343815 1106 78-79 0.08819485481590306 1106 80-81 0.01961507286444686 1106 82-83 0.046862169604068526 1106 84-85 0.07322641955020259 1106 86-87 0.07505332168495471 1106 88-89 0.05503564663333549 1106 90-91 0.12266220502888103 1106 92-93 0.17091465108070025 1106 94-95 0.1287376243318903 1106 96-97 0.16770700839582986 1106 98-99 0.18325219624102118 1106 100-101 0.1375373820997936 1107 1 0.0031277612831033252 1107 2 0.09837397323591546 1107 3 0.07167909595487743 1107 4 0.08264968484832025 1107 5 0.10248083785722173 1107 6 0.10582136136665099 1107 7 0.0912414983977925 1107 8 0.10880876559362918 1107 9 0.14480077145066161 1107 10-11 0.026108086317712775 1107 12-13 0.09488641485108928 1107 14-15 0.10720559320677836 1107 16-17 0.05540804681842815 1107 18-19 0.04403125486731341 1107 20-21 0.017017325593172927 1107 22-23 0.06091542690352725 1107 24-25 0.09337388244531297 1107 26-27 -0.052251193077552216 1107 28-29 0.04026565433634488 1107 30-31 0.07897999104131515 1107 32-33 0.06958399954649508 1107 34-35 0.09334663331816984 1107 36-37 0.07119501674635131 1107 38-39 0.05989829296618154 1107 40-41 0.0720475209294662 1107 42-43 0.11758509666096018 1107 44-45 0.1235982392073538 1107 46-47 0.16683622979994794 1107 48-49 0.13198263425940837 1107 50-51 0.13451340231419096 1107 52-53 0.17992735900462264 1107 54-55 0.07840799587236091 1107 56-57 0.07664002627665667 1107 58-59 -0.03513036922210233 1107 60-61 0.08502512123596517 1107 62-63 -0.1781522048589963 1107 64-65 -0.030623411579135507 1107 66-67 -0.01851748486689786 1107 68-69 0.0030306201884258144 1107 70-71 0.08797081609030499 1107 72-73 -0.040820794376301706 1107 74-75 0.011462911584381175 1107 76-77 0.02888976129119669 1107 78-79 -0.025779134017923866 1107 80-81 0.017344081100873154 1107 82-83 0.021464457126469938 1107 84-85 0.04168003859610536 1107 86-87 -0.0642398966552804 1107 88-89 -0.032249505916468024 1107 90-91 -0.025536200935292186 1107 92-93 0.028765208056540814 1107 94-95 0.07840364244124487 1107 96-97 0.10717273900949209 1107 98-99 -0.0032386988479373713 1107 100-101 -0.0036939021765718394 1108 1 -0.03867899627004334 1108 2 0.026556565256434794 1108 3 0.027959030506266913 1108 4 -0.013363021386773255 1108 5 -0.013532933695607596 1108 6 0.016572872532577776 1108 7 0.01972145436558037 1108 8 -0.03867001453572527 1108 9 -0.17567843858839183 1108 10-11 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2214 84-85 -0.39578623423618353 2214 86-87 -0.3986672270436884 2214 88-89 -0.34322091836953916 2214 90-91 -0.3958657065157567 2214 92-93 -0.3757308317555541 2214 94-95 -0.3349303709535718 2214 96-97 -0.3469686260972935 2214 98-99 -0.4165099578285947 2214 100-101 -0.6889025193128404 2215 1 -0.5334949873031078 2215 2 -0.9147172400926102 2215 3 -0.5463428350611927 2215 4 -0.3072765523341374 2215 5 -0.3108316556234101 2215 6 -0.3256357844233406 2215 7 -0.30876926461694865 2215 8 -0.4296480433926604 2215 9 -0.44746725741920557 2215 10-11 -0.5083981567249154 2215 12-13 -0.3799307196312256 2215 14-15 -0.5409820087883972 2215 16-17 -0.5258740389123275 2215 18-19 -0.4185254500738722 2215 20-21 -0.44970940525711 2215 22-23 -0.5304793570383382 2215 24-25 -0.49331652925798153 2215 26-27 -0.5853351806009854 2215 28-29 -0.5139755624131865 2215 30-31 -0.518182729259081 2215 32-33 -0.5342784207452453 2215 34-35 -0.6008478961374877 2215 36-37 -0.5475371922136034 2215 38-39 -0.5420172687127902 2215 40-41 -0.5754520959617508 2215 42-43 -0.6212250736013942 2215 44-45 -0.528814429086296 2215 46-47 -0.5340879803546557 2215 48-49 -0.599364731151816 2215 50-51 -0.5732672395401508 2215 52-53 -0.6370874573938892 2215 54-55 -0.6448688575233206 2215 56-57 -0.5521816097782661 2215 58-59 -0.5216677172587509 2215 60-61 -0.48309880347542133 2215 62-63 -0.4599301541019116 2215 64-65 -0.3294375570026844 2215 66-67 -0.33153494604998457 2215 68-69 -0.40679260905355363 2215 70-71 -0.36002360500792463 2215 72-73 -0.3935910433798142 2215 74-75 -0.45300871470951876 2215 76-77 -0.3509400526390891 2215 78-79 -0.2822096202986799 2215 80-81 -0.25140083754468634 2215 82-83 -0.3745944256947311 2215 84-85 -0.34735058176580225 2215 86-87 -0.22611204144743624 2215 88-89 -0.22812834045787156 2215 90-91 -0.19138786469459745 2215 92-93 -0.2460989568080194 2215 94-95 -0.25585137531535906 2215 96-97 -0.3132129484655266 2215 98-99 -0.3117848546907709 2215 100-101 -0.36664685048963364 2216 1 -0.5163271542706376 2216 2 -0.5492600769030567 2216 3 -0.5242924766338213 2216 4 -0.32753923879467806 2216 5 -0.3971063189709483 2216 6 -0.40434555579366815 2216 7 -0.38466581387769594 2216 8 -0.4547455153050848 2216 9 -0.5823119892649729 2216 10-11 -0.6926320920623681 2216 12-13 -0.7374050087525603 2216 14-15 -1.045368931753302 2216 16-17 -1.3019228751567695 2216 18-19 -1.3803695582093027 2216 20-21 -1.678014348779726 2216 22-23 -1.4102794537546544 2216 24-25 -1.5880732753584184 2216 26-27 -1.3005418636434882 2216 28-29 -1.348031348769041 2216 30-31 -1.4783938909264478 2216 32-33 -1.1781997521882062 2216 34-35 -0.7490191128672166 2216 36-37 -1.1252779114313043 2216 38-39 -1.432643513729289 2216 40-41 -1.4667542561426572 2216 42-43 -0.7911279245346208 2216 44-45 -1.2948981532689459 2216 46-47 -0.9230127600640117 2216 48-49 -0.919478221704118 2216 50-51 -1.0353333899285317 2216 52-53 -1.0681722737565238 2216 54-55 -1.328248625678924 2216 56-57 -1.3390179558797968 2216 58-59 -1.0522683338252676 2216 60-61 -1.149197227439096 2216 62-63 -1.0187318540560213 2216 64-65 -1.1042532258701954 2216 66-67 -0.8544411889233388 2216 68-69 -0.8519258481434306 2216 70-71 -0.807344466882185 2216 72-73 -0.7588756215235719 2216 74-75 -0.7245145286789807 2216 76-77 -0.622605228720488 2216 78-79 -0.5317852843303754 2216 80-81 -0.550291690983741 2216 82-83 -0.704464552442559 2216 84-85 -0.588028241670564 2216 86-87 -0.7710363581823856 2216 88-89 -0.7334070386892222 2216 90-91 -0.7312522626492708 2216 92-93 -0.6676387039168361 2216 94-95 -0.8030536429283046 2216 96-97 -0.7620778025255532 2216 98-99 -1.1046946488663671 2216 100-101 -0.8414435951063552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 5.0 12 51.0 13 205.0 14 664.0 15 1426.0 16 2567.0 17 3962.0 18 7465.0 19 16394.0 20 19437.0 21 17296.0 22 15651.0 23 15216.0 24 16316.0 25 19809.0 26 25684.0 27 34490.0 28 45978.0 29 60845.0 30 80911.0 31 105980.0 32 139751.0 33 185048.0 34 252221.0 35 360979.0 36 596374.0 37 1117988.0 38 1328318.0 39 307743.0 40 443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.97176841163026 40.41518481777679 12.430310295338927 22.18273647525402 2 24.80046434345681 27.501615849110138 29.868758946280156 17.829160861152896 3 22.824514706476222 27.070604184238395 24.441912186107317 25.662968923178063 4 23.569962706874694 26.65166931531028 25.963705767791268 23.814662210023762 5 24.01152823859788 27.076672118740692 25.07245427654423 23.839345366117197 6 24.166637198823825 25.618380066542223 26.004154233503744 24.210828501130205 7 24.497318705286908 26.933259625365725 24.746187715529373 23.823233953817997 8 24.111942092654846 25.255036333327123 26.56840937048109 24.064612203536935 9 23.591585323214808 24.951493140827765 26.82776126067407 24.629160275283358 10-11 24.514465851233915 25.621874359859063 25.657340390829386 24.206319398077635 12-13 24.357766673690126 25.809980329430253 25.431205211058455 24.401047785821163 14-15 24.10870934823897 25.819406551820894 25.608169197448703 24.463714902491432 16-17 24.234399288084816 26.007156814886777 25.554290123850727 24.204153773177676 18-19 23.787071948979122 25.94924485585514 26.167000054402184 24.096683140763556 20-21 23.31079688509906 26.122133711749257 26.369956526387877 24.197112876763807 22-23 23.15092773487562 26.18646427942964 26.847952835828266 23.814655149866475 24-25 23.13496278996096 25.732886175382973 27.717979561941164 23.414171472714905 26-27 23.0273736176741 25.655763593985082 28.13949602848248 23.177366759858334 28-29 23.04931390850307 25.821142596908548 28.07468473604264 23.054858758545738 30-31 23.121233498482336 25.76917632680399 28.010818502895713 23.09877167181796 32-33 23.049466868959133 25.786221556283568 28.124312779975135 23.03999879478216 34-35 23.12553656757032 25.912005324717146 28.042770987154604 22.91968712055793 36-37 23.122690915527865 25.84132434972143 27.94950264907189 23.086482085678817 38-39 23.127942034043638 25.875033138259617 27.93051542521906 23.066509402477685 40-41 23.19094805507849 26.08433056802622 27.619111586502893 23.105609790392396 42-43 23.26778317487462 26.10381348152223 27.428298640226622 23.200104703376525 44-45 23.247970138975997 26.096905417248657 27.510999995501344 23.144124448274 46-47 23.294186031426925 26.333012081066048 27.288796860739023 23.084005026768 48-49 23.362512777972892 26.29336729703097 27.11661870382419 23.227501221171945 50-51 23.27857505513718 26.304562683025555 27.175081940732337 23.241780321104937 52-53 23.323300870112522 26.44176205318433 27.095005557471286 23.13993151923186 54-55 23.40590542914436 26.357552872318955 26.956264554671893 23.28027714386479 56-57 23.370031115975692 26.41456749086723 26.95594278309606 23.25945861006102 58-59 23.42706082682907 26.537190317824795 26.804767936886165 23.230980918459966 60-61 23.49155351151499 26.478378726168895 26.641135921141696 23.388931841174422 62-63 23.499668930188893 26.481285009102457 26.696446384842158 23.32259967586649 64-65 23.566455170786053 26.658221059733613 26.520143860581157 23.25517990889918 66-67 23.596997330523386 26.533452990387126 26.453471219326026 23.41607845976347 68-69 23.588318553443887 26.57154655327775 26.437475590767505 23.402659302510855 70-71 23.599793982191784 26.683072485899046 26.387583608423043 23.329549923486123 72-73 23.628154229415774 26.591044811096708 26.291602517399287 23.489198442088224 74-75 23.600419486012463 26.614439458468 26.38220306320203 23.40293799231751 76-77 23.629330872944042 26.737138169557827 26.249001007388383 23.38452995010974 78-79 23.669818717166432 26.650986971296764 26.19876854304795 23.480425768488857 80-81 23.590039421700673 26.674130226885325 26.243882239792033 23.49194811162197 82-83 23.63001233594919 26.834597111623964 26.17266583408687 23.362724718339972 84-85 23.668564395745666 26.76235311152173 26.092746077237273 23.47633641549533 86-87 23.629070511700547 26.737631509402892 26.17564661688589 23.45765136201067 88-89 23.671691106044488 26.818521024358333 26.10640490215269 23.403382967444493 90-91 23.70219934861626 26.744752088237167 26.047963361312505 23.50508520183407 92-93 23.639822752854688 26.720399480875734 26.148609522195386 23.491168244074196 94-95 23.70352825282614 26.92496329422724 25.95193861091588 23.41956984203074 96-97 23.715844376171617 26.823378684802513 25.921521027303573 23.539255911722297 98-99 23.664531032260495 26.824052878921666 26.029634542875197 23.481781545942642 100-101 23.729589408385934 26.906129876342373 25.902739798980896 23.461540916290797 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1068.0 1 731.5 2 300.0 3 173.0 4 126.5 5 109.0 6 102.0 7 96.0 8 98.0 9 109.0 10 109.0 11 105.5 12 123.5 13 188.0 14 281.0 15 393.5 16 1288.0 17 1636.5 18 1325.5 19 1749.5 20 2458.5 21 3590.0 22 4936.0 23 6525.0 24 8677.5 25 11525.0 26 15065.5 27 19378.0 28 24400.5 29 30140.5 30 36989.5 31 44434.5 32 52173.5 33 60934.0 34 70306.0 35 80777.0 36 92183.0 37 104323.0 38 117737.5 39 131568.0 40 143968.5 41 153107.0 42 162108.0 43 172774.5 44 182461.5 45 190925.0 46 197926.5 47 201994.5 48 201834.0 49 199410.5 50 196994.5 51 194905.5 52 193665.0 53 189870.5 54 179386.5 55 165905.0 56 151112.5 57 133680.5 58 117743.5 59 103018.0 60 86029.0 61 69840.5 62 56403.5 63 44577.0 64 35213.5 65 27721.5 66 21368.5 67 16069.5 68 12503.5 69 10347.5 70 8600.5 71 7324.0 72 6344.5 73 5295.5 74 4195.5 75 2842.0 76 1653.0 77 1070.5 78 696.5 79 360.0 80 138.0 81 60.5 82 34.5 83 16.0 84 10.5 85 5.5 86 2.0 87 2.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.040445922044617735 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.0923912076884497E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.230978019221125E-5 28-29 2.406249888841717E-4 30-31 3.138586811532675E-5 32-33 4.1847824153768994E-5 34-35 0.0 36-37 0.0 38-39 4.1847824153768994E-5 40-41 1.046195603844225E-4 42-43 1.1508151642286472E-4 44-45 3.033967251148252E-4 46-47 1.4646738453819147E-4 48-49 1.0461956038442249E-5 50-51 9.415760434598023E-5 52-53 0.0014437499333050303 54-55 8.892662632675912E-4 56-57 8.369564830753799E-5 58-59 9.834238676135715E-4 60-61 3.3478259323015195E-4 62-63 3.661684613454787E-4 64-65 2.197010768072872E-4 66-67 1.4646738453819147E-4 68-69 7.427988787293997E-4 70-71 4.7078802172990117E-4 72-73 6.27717362306535E-5 74-75 8.160325709984953E-4 76-77 6.591032304218616E-4 78-79 8.369564830753799E-5 80-81 1.0461956038442249E-5 82-83 0.0020819292516500074 84-85 0.0 86-87 0.0 88-89 3.033967251148252E-4 90-91 2.197010768072872E-4 92-93 3.8709237342236314E-4 94-95 0.0 96-97 6.277173623065349E-4 98-99 2.0923912076884497E-5 100-101 0.008819428940406816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4779221.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.89380988646803 #Duplication Level Percentage of deduplicated Percentage of total 1 81.90761852944657 55.61020280691592 2 10.792227425290713 14.654508741284284 3 3.2793275746624446 6.679381287287529 4 1.3892261174466796 3.772794156289639 5 0.7369481591142936 2.501710910554422 6 0.43454303386133075 1.770166927708214 7 0.3038697593378748 1.4441612969512727 8 0.20109454718954362 1.0922459964873799 9 0.15198577719617942 0.9287004116164045 >10 0.7789447132554335 9.267078595738665 >50 0.021354018097589623 0.9433782944664258 >100 0.0025213860047915222 0.2606392024709092 >500 9.244339717428484E-5 0.05069186034504624 >1k 1.540723121067585E-4 0.1925579197618835 >5k 3.081446242133436E-5 0.11584020010008186 >10k+ 6.162892484266872E-5 0.7159413920218352 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22693 0.4748263367607398 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 11528 0.24121085842232448 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5537 0.11585570116970946 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.0923912076884497E-5 0.0 0.0 0.0 3 0.0 2.0923912076884497E-5 0.0 0.0 0.0 4 0.0 2.0923912076884497E-5 0.0 2.0923912076884497E-5 0.0 5 0.0 2.0923912076884497E-5 0.0 2.0923912076884497E-5 0.0 6 0.0 2.0923912076884497E-5 0.0 2.0923912076884497E-5 0.0 7 0.0 2.0923912076884497E-5 0.0 4.1847824153768994E-5 0.0 8 8.369564830753799E-5 2.0923912076884497E-5 0.0 4.1847824153768994E-5 0.0 9 1.8831520869196046E-4 2.0923912076884497E-5 0.0 4.1847824153768994E-5 0.0 10-11 3.661684613454787E-4 2.0923912076884497E-5 0.0 4.1847824153768994E-5 0.0 12-13 6.904890985371884E-4 2.0923912076884497E-5 0.0 4.1847824153768994E-5 0.0 14-15 8.997282193060333E-4 6.27717362306535E-5 0.0 4.1847824153768994E-5 0.0 16-17 0.0010775814719595515 6.27717362306535E-5 0.0 4.1847824153768994E-5 0.0 18-19 0.0012972825487668387 8.369564830753799E-5 0.0 4.1847824153768994E-5 0.0 20-21 0.0015274455816125683 1.3600542849974922E-4 0.0 4.1847824153768994E-5 0.0 22-23 0.0019249999110733736 3.033967251148252E-4 0.0 4.1847824153768994E-5 2.0923912076884497E-5 24-25 0.0023225542405341795 4.7078802172990117E-4 0.0 4.1847824153768994E-5 2.0923912076884497E-5 26-27 0.002741032482071869 8.160325709984954E-4 0.0 6.27717362306535E-5 2.0923912076884497E-5 28-29 0.003159510723609559 0.0010357336478057826 0.0 9.415760434598023E-5 2.0923912076884497E-5 30-31 0.0035256791849550377 0.0010880434279979937 0.0 1.0461956038442248E-4 4.1847824153768994E-5 32-33 0.003787228085916094 0.0010880434279979937 0.0 1.0461956038442248E-4 4.1847824153768994E-5 34-35 0.0041010867670693615 0.0011089673400748784 0.0 1.0461956038442248E-4 4.1847824153768994E-5 36-37 0.004477717184453282 0.0011089673400748784 0.0 1.0461956038442248E-4 4.1847824153768994E-5 38-39 0.004990353030336953 0.0011089673400748784 0.0 1.0461956038442248E-4 4.1847824153768994E-5 40-41 0.005555298656412834 0.0011089673400748784 0.0 1.0461956038442248E-4 4.1847824153768994E-5 42-43 0.006319021447219119 0.0011089673400748784 0.0 1.0461956038442248E-4 4.1847824153768994E-5 44-45 0.007176901842371382 0.0011089673400748784 0.0 1.0461956038442248E-4 4.1847824153768994E-5 46-47 0.008202173534138723 0.0011089673400748784 0.0 1.0461956038442248E-4 4.1847824153768994E-5 48-49 0.009593613687251541 0.0011089673400748784 0.0 1.1508151642286474E-4 4.1847824153768994E-5 50-51 0.011204754917171648 0.0011089673400748784 0.0 1.3600542849974922E-4 4.1847824153768994E-5 52-53 0.033436411498861424 0.0011089673400748784 0.0 1.4646738453819147E-4 4.1847824153768994E-5 54-55 0.06712390994264547 0.0011089673400748784 0.0 1.4646738453819147E-4 4.1847824153768994E-5 56-57 0.10575991359261269 0.0011089673400748784 0.0 1.4646738453819147E-4 4.1847824153768994E-5 58-59 0.15163559082118194 0.0011089673400748784 0.0 1.7785325265351823E-4 4.1847824153768994E-5 60-61 0.20407091448585452 0.0011089673400748784 0.0 2.0923912076884496E-4 4.1847824153768994E-5 62-63 0.26586968880493284 0.0011089673400748784 0.0 2.0923912076884496E-4 4.1847824153768994E-5 64-65 0.33480351714222883 0.0011089673400748784 0.0 2.0923912076884496E-4 4.1847824153768994E-5 66-67 0.41150011686004895 0.0011089673400748784 0.0 2.1970107680728722E-4 4.1847824153768994E-5 68-69 0.4975601672322749 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 70-71 0.5948040486095956 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 72-73 0.7014636904215144 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 74-75 0.8216297174790619 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 76-77 0.953962999409318 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 78-79 1.098170601443206 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 80-81 1.2564286104367217 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 82-83 1.429270586147826 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 84-85 1.621163783804934 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 86-87 1.8284360568385516 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 88-89 2.0493716444583754 0.0011089673400748784 0.0 2.51086944922614E-4 4.1847824153768994E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3185 0.0 69.674736 1 TCGACGG 3415 0.0 65.53874 2 TATCTCG 3085 0.0 58.070297 2 ATATCTC 3450 0.0 52.477573 1 CGACGGC 4330 0.0 51.68933 3 ATCTCGA 6900 0.0 49.03416 1 ACGGCGC 4915 0.0 45.710358 5 TCTCGAG 7245 0.0 45.256256 2 GACGGCG 5055 0.0 44.275925 4 CGGCGCG 5040 0.0 44.199696 6 CTCGAGG 8250 0.0 39.68562 3 TCGAGGG 8325 0.0 39.271008 4 GGGCGCG 8400 0.0 38.790184 8 AGGGCGC 8845 0.0 37.053417 7 CGAGGGC 9025 0.0 36.36703 5 GAGGGCG 9450 0.0 34.832005 6 GGCGCGC 13290 0.0 25.410994 9 CGCGCCG 13235 0.0 23.542736 9 GCGCGCC 13330 0.0 23.33932 8 TCCATAT 6705 0.0 18.985107 18-19 >>END_MODULE