FastQCFastQC Report
Tue 31 May 2016
SRR2048236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199427
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT643271.2371940215719923No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT71010.13657274157325414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG129250.087.321741
TATCTCG143000.086.0971452
ATATCTC147100.083.9589841
ATCTCGA153500.079.554873
TCTCGAG133200.075.632684
TCGAGGG134650.074.782946
CTCGAGG135550.074.321465
AGGGCGC135650.073.986559
TCGACGG153200.073.884792
CGAGGGC136750.073.3219457
GAGGGCG138450.072.627498
CGACGGC161200.070.392143
GACGGCG170400.066.591634
CGGCGCG178600.066.406436
ACGGCGC171250.066.1778955
CTCGACG33300.063.7582445
TCTCGAC35100.060.4885944
GGCGCGC313550.038.7041177
CGCGCCG315750.038.359239
GCGCGCC319550.037.888218
Produced by FastQC (version 0.11.5)