FastQCFastQC Report
Tue 31 May 2016
SRR2048236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199427
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT151320.29103206949535015No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT111850.21511985839978137No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94570.1818854269903203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG35150.065.724761
TATCTCG32750.062.0880782
TCGACGG42800.056.944182
ATATCTC37450.052.2954031
CGACGGC51750.048.013453
CGGCGCG59650.044.3546
ATCTCGA64100.044.198371
GACGGCG62650.042.0054784
ACGGCGC63450.041.8473745
TCTCGAG64450.039.9532472
TCGAGGG72050.037.3823284
GGGCGCG76400.037.116678
CTCGAGG69950.036.6077163
CGAGGGC78200.036.201585
AGGGCGC80000.035.505797
GAGGGCG81850.034.645256
GGCGCGC136500.025.124227
CGCGCCG136850.025.0252559
GCGCGCC138300.024.7972248
TCGCGAG1951.569424E-824.3635832
Produced by FastQC (version 0.11.5)