##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048236_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5199427 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.774734600562716 31.0 28.0 33.0 16.0 34.0 2 30.664529956858708 31.0 30.0 33.0 28.0 34.0 3 28.476704836898374 31.0 28.0 33.0 16.0 34.0 4 32.9005855837576 35.0 33.0 37.0 19.0 37.0 5 34.180582591120135 35.0 33.0 37.0 30.0 37.0 6 34.813833716676854 35.0 35.0 37.0 32.0 37.0 7 35.009735688182566 35.0 35.0 37.0 33.0 37.0 8 35.17512525899488 35.0 35.0 37.0 33.0 37.0 9 36.93398849527073 39.0 37.0 39.0 33.0 39.0 10-11 36.946968098600095 39.0 37.0 39.0 33.5 39.0 12-13 36.90546717936419 39.0 37.0 39.0 33.5 39.0 14-15 37.8956423467432 39.0 37.0 40.0 34.0 41.0 16-17 37.850601422041315 39.0 37.0 40.0 34.0 41.0 18-19 37.9981764336724 39.0 37.0 40.0 34.0 41.0 20-21 38.07122159807225 39.0 37.0 40.0 34.0 41.0 22-23 38.04452394465775 39.0 37.0 40.0 34.0 41.0 24-25 37.99732970575411 39.0 37.0 40.0 34.0 41.0 26-27 38.24920121005641 39.0 37.5 40.0 34.5 41.0 28-29 38.45337592007735 39.0 38.0 40.0 35.0 41.0 30-31 38.404464953541996 39.0 38.0 40.0 35.0 41.0 32-33 38.377865676352414 39.0 38.0 40.0 35.0 41.0 34-35 38.36098266212797 39.0 38.0 40.0 35.0 41.0 36-37 38.356524670891616 40.0 38.0 40.0 35.0 41.0 38-39 38.29258070168117 39.0 38.0 40.0 35.0 41.0 40-41 38.20990274505249 39.0 38.0 40.0 35.0 41.0 42-43 38.13375916230769 39.0 37.5 40.0 34.5 41.0 44-45 38.03114458573992 39.0 37.0 40.0 34.0 41.0 46-47 37.91596968281313 39.0 37.0 40.0 34.0 41.0 48-49 37.817307464841804 39.0 37.0 40.0 34.0 41.0 50-51 37.688841385791164 39.0 37.0 40.0 33.5 41.0 52-53 37.542145797988894 39.0 36.0 40.0 33.0 41.0 54-55 37.3571166592011 39.0 36.0 40.0 33.0 41.0 56-57 37.115080084786264 38.0 36.0 40.0 33.0 41.0 58-59 36.8489263336133 38.0 35.0 40.0 33.0 40.0 60-61 36.50282406118982 38.0 35.0 40.0 32.0 40.0 62-63 36.206926840207586 37.5 35.0 39.0 32.0 40.0 64-65 35.89731435790905 37.0 34.5 39.0 31.5 40.0 66-67 35.54687910802479 36.0 34.0 39.0 31.0 40.0 68-69 35.18530609238287 36.0 34.0 38.0 31.0 40.0 70-71 34.80915127763117 35.5 34.0 38.0 31.0 39.5 72-73 34.42584375162879 35.0 33.5 37.0 30.0 39.0 74-75 34.031090925980884 35.0 33.0 36.5 30.0 39.0 76-77 33.63870249548653 34.5 33.0 36.0 30.0 38.0 78-79 33.25542391498141 34.0 32.5 35.5 29.0 37.0 80-81 32.82553135182012 34.0 32.0 35.0 29.0 37.0 82-83 32.56095565915244 34.0 32.0 35.0 29.0 36.0 84-85 32.22971069696719 34.0 31.5 35.0 28.5 36.0 86-87 31.974290628563494 34.0 31.0 35.0 28.0 35.0 88-89 31.688589338786755 34.0 31.0 34.0 27.0 35.0 90-91 31.41865026665438 33.5 31.0 34.0 27.0 35.0 92-93 31.135927862820267 33.0 30.5 34.0 26.0 35.0 94-95 30.838700976088326 33.0 30.0 34.0 25.5 35.0 96-97 30.476726570062432 33.0 30.0 34.0 25.0 35.0 98-99 29.987910783245923 32.0 30.0 34.0 24.5 35.0 100-101 27.850954345546153 30.5 26.5 33.0 20.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.026278330463448 1101 2 1.5028797581828144 1101 3 2.6557031906979844 1101 4 1.7322767915707615 1101 5 0.5784458692964733 1101 6 0.6654950073231021 1101 7 0.25552076471138463 1101 8 0.4860699652621463 1101 9 0.6191494341110086 1101 10-11 0.5297645415156396 1101 12-13 0.3971126586578393 1101 14-15 0.2703500872457596 1101 16-17 0.19507986482486928 1101 18-19 0.17328960576355001 1101 20-21 0.15279586234029097 1101 22-23 0.14105182146900574 1101 24-25 0.14616809758670968 1101 26-27 0.1407133311047133 1101 28-29 0.15264321255097713 1101 30-31 0.14624481849265436 1101 32-33 0.13381048195397938 1101 34-35 0.10108200615227503 1101 36-37 0.08625770968461666 1101 38-39 0.12002367423948357 1101 40-41 0.09656028103672298 1101 42-43 0.1132994795046045 1101 44-45 0.10495568686857837 1101 46-47 0.11310160297324501 1101 48-49 0.10928209156508473 1101 50-51 0.11317775955527054 1101 52-53 0.02587735512836531 1101 54-55 0.0392753768516485 1101 56-57 0.058117259619130834 1101 58-59 0.10724424105899999 1101 60-61 0.09656328617869292 1101 62-63 0.12984896357517073 1101 64-65 0.1260346394554972 1101 66-67 0.03940455246567609 1101 68-69 0.04696632405784129 1101 70-71 0.04394041577817376 1101 72-73 0.07571227244716994 1101 74-75 0.06900982985466442 1101 76-77 0.027902900032437117 1101 78-79 0.052584622358651245 1101 80-81 -0.018898873445209574 1101 82-83 0.07933928933426415 1101 84-85 0.14557483550741068 1101 86-87 0.049667314849259014 1101 88-89 0.014130213545151094 1101 90-91 0.05833930281658439 1101 92-93 0.10100817569339782 1101 94-95 0.045790983534729435 1101 96-97 0.049771990662193843 1101 98-99 0.13474827248166577 1101 100-101 0.18119332250411446 1102 1 0.5640467940258311 1102 2 0.7776865052100845 1102 3 -0.8652906638025719 1102 4 1.2268867319984835 1102 5 1.1174012285265675 1102 6 0.8631837378546408 1102 7 0.8717376975470472 1102 8 0.6545361948248711 1102 9 0.7536024344546064 1102 10-11 0.6038709965650142 1102 12-13 0.4369356362676342 1102 14-15 0.27649087990064913 1102 16-17 0.16777498874682095 1102 18-19 0.12288144858185035 1102 20-21 0.07306538100819893 1102 22-23 0.08697629003313523 1102 24-25 0.0680359638573762 1102 26-27 0.019409730566884775 1102 28-29 0.01720472794924177 1102 30-31 -8.333628662597903E-4 1102 32-33 0.046008835518669855 1102 34-35 0.06047162171731202 1102 36-37 0.019200065621532758 1102 38-39 0.09683233693048265 1102 40-41 0.06453920089106191 1102 42-43 0.06287073011452549 1102 44-45 0.03156211446166424 1102 46-47 0.027484691788814075 1102 48-49 -0.04162722113368744 1102 50-51 -0.012522375529620433 1102 52-53 -0.05893940167334222 1102 54-55 -0.010885869945781224 1102 56-57 0.04208142054743291 1102 58-59 0.02249318320784255 1102 60-61 0.03555582905363508 1102 62-63 0.006863077670907103 1102 64-65 0.0483663207053695 1102 66-67 -0.05605386539377122 1102 68-69 -0.12509726625056317 1102 70-71 -0.12597668195205358 1102 72-73 -0.04742638803163857 1102 74-75 0.01708530167118738 1102 76-77 -0.009579626007884201 1102 78-79 -3.759399710645539E-6 1102 80-81 -0.028158503146315184 1102 82-83 0.00689707695445918 1102 84-85 0.02145500532840572 1102 86-87 -0.0026006129234730224 1102 88-89 1.2751184741333077E-4 1102 90-91 0.04087789457625135 1102 92-93 0.03402525192928252 1102 94-95 -0.004203564432955886 1102 96-97 -0.10347640079215736 1102 98-99 0.006250146568746118 1102 100-101 -0.03326256467097721 1103 1 0.29889707408656463 1103 2 0.703910196362461 1103 3 1.1641890786533153 1103 4 2.017447098662366 1103 5 1.3703460972425319 1103 6 0.9423681940945059 1103 7 0.7756841854246588 1103 8 0.590028154777734 1103 9 0.6228909455045013 1103 10-11 0.4193014774141446 1103 12-13 0.24965907499755957 1103 14-15 0.1174566146874696 1103 16-17 0.07490803221829623 1103 18-19 -0.029673880495003857 1103 20-21 -0.005344716145494033 1103 22-23 0.05332239895360402 1103 24-25 0.004994764088387171 1103 26-27 -0.052594429698039846 1103 28-29 -0.06927064128731786 1103 30-31 0.025851283126392843 1103 32-33 0.05832395766954335 1103 34-35 0.04379668473107756 1103 36-37 -0.0025971316599537886 1103 38-39 -0.03109162969421675 1103 40-41 0.05959957182787434 1103 42-43 0.015157854844282781 1103 44-45 0.03385301827354681 1103 46-47 0.008794594866202488 1103 48-49 0.06102711882999046 1103 50-51 0.0424236283219912 1103 52-53 0.025965192543090154 1103 54-55 -0.06300801158396041 1103 56-57 0.0337353462896246 1103 58-59 0.05864526188537411 1103 60-61 -0.01646590534086556 1103 62-63 0.0788925294329843 1103 64-65 -0.06836871420262014 1103 66-67 -0.10175257907621216 1103 68-69 -0.08613174811870294 1103 70-71 -0.09485647974573652 1103 72-73 -0.08762710295037834 1103 74-75 -0.04296809373759913 1103 76-77 -0.06209129663613311 1103 78-79 -0.034693723315669445 1103 80-81 -0.14764638440598787 1103 82-83 -0.22108235525463016 1103 84-85 -0.13755750919722942 1103 86-87 -0.10925220745519582 1103 88-89 -0.2575780019998035 1103 90-91 -0.12076790407612137 1103 92-93 -0.15349095293194992 1103 94-95 -0.20299780694875835 1103 96-97 -0.2597816202836185 1103 98-99 -0.30193420054496656 1103 100-101 -0.5165368849546681 1104 1 -0.020993080973429556 1104 2 -0.3116361249964683 1104 3 0.9626355673392517 1104 4 -0.06974600467750491 1104 5 0.050699813759138124 1104 6 0.2135634127758479 1104 7 0.35031183775497254 1104 8 0.26342699994067686 1104 9 0.2371856100584111 1104 10-11 0.16443082926102903 1104 12-13 0.038596318753100434 1104 14-15 0.0345851802812831 1104 16-17 -0.021860141986728365 1104 18-19 -0.07189914447639012 1104 20-21 0.013398677280257232 1104 22-23 -0.014439098852733423 1104 24-25 -0.08529092046853037 1104 26-27 -0.09703386001194048 1104 28-29 -0.06113756615282995 1104 30-31 -0.05524627539222848 1104 32-33 -0.02431204914291385 1104 34-35 -0.025158777123763798 1104 36-37 -0.03905921918772037 1104 38-39 0.04893320606721829 1104 40-41 -0.008355278058253646 1104 42-43 -0.027332301280146964 1104 44-45 -0.020803418136566165 1104 46-47 -0.02520090762648408 1104 48-49 0.04092499367066438 1104 50-51 0.09054565284086635 1104 52-53 0.056609114585540965 1104 54-55 0.06348329858812463 1104 56-57 0.0672463993325394 1104 58-59 0.04452228798227509 1104 60-61 -0.04775380484801417 1104 62-63 -0.027745794331352158 1104 64-65 0.025752979625679018 1104 66-67 0.008215335026648063 1104 68-69 -0.06678896255795053 1104 70-71 0.005972431945480139 1104 72-73 0.008487831538410262 1104 74-75 0.028774350042631625 1104 76-77 0.020206957738189146 1104 78-79 0.03402235024990574 1104 80-81 -0.08227009859471224 1104 82-83 -0.014399619441029188 1104 84-85 -0.03883522682882301 1104 86-87 -0.09022400151316745 1104 88-89 -0.032531466921472685 1104 90-91 0.05765172091724935 1104 92-93 0.09740486219576283 1104 94-95 -0.0031586648222088343 1104 96-97 -0.011969983647343696 1104 98-99 -0.12512244603674105 1104 100-101 -0.3491457066806163 1105 1 -0.8840039699976927 1105 2 -0.5264081006452486 1105 3 -1.545103272220686 1105 4 -0.33944961250365324 1105 5 -0.3374293032860436 1105 6 -0.4602089914359766 1105 7 -0.5216580938675008 1105 8 -0.3962109142902861 1105 9 -0.5364428501105252 1105 10-11 -0.40069158736285715 1105 12-13 -0.42168939591645227 1105 14-15 -0.39531981784459447 1105 16-17 -0.3206180107312022 1105 18-19 -0.23608111076426042 1105 20-21 -0.12835090188157494 1105 22-23 -0.12151695716002564 1105 24-25 -0.18703120588764222 1105 26-27 -0.06725023583739898 1105 28-29 -0.018967499617325245 1105 30-31 -0.014835601231652618 1105 32-33 -0.059231384202938386 1105 34-35 -0.10594146371401791 1105 36-37 -0.006522834991017135 1105 38-39 -0.03604818928091902 1105 40-41 0.007323053952269731 1105 42-43 -0.004577842530203213 1105 44-45 -0.07063949389027613 1105 46-47 0.00123152069892285 1105 48-49 -0.02927117057810591 1105 50-51 -0.01686635328351116 1105 52-53 0.011314284554607923 1105 54-55 0.07466458463851211 1105 56-57 0.06940494795623664 1105 58-59 0.05217820633944825 1105 60-61 0.08392558759855717 1105 62-63 0.06777050472296509 1105 64-65 0.12808862584606118 1105 66-67 0.06466082026943099 1105 68-69 0.10210279370117803 1105 70-71 0.020549531572285673 1105 72-73 0.1067050182153082 1105 74-75 0.05291828751750671 1105 76-77 0.05979483861408852 1105 78-79 0.10544901738030887 1105 80-81 -0.02250286908390109 1105 82-83 0.014888507630963943 1105 84-85 0.0524856773302389 1105 86-87 0.0687370786627568 1105 88-89 0.040329789262354865 1105 90-91 0.09114212437405556 1105 92-93 0.08332524500362481 1105 94-95 0.03691723457429674 1105 96-97 0.019044591228116303 1105 98-99 0.12303809896528861 1105 100-101 0.06346545139346915 1106 1 0.23697482020110883 1106 2 0.4507028848884822 1106 3 1.2550728248207115 1106 4 1.0703095639997215 1106 5 0.9537980687453995 1106 6 0.7182756205837961 1106 7 0.6391623297065436 1106 8 0.4984587896940056 1106 9 0.5667414639744095 1106 10-11 0.4118433936893737 1106 12-13 0.23637954974778097 1106 14-15 0.06052291299147328 1106 16-17 0.03389699506095667 1106 18-19 -0.10474298807697835 1106 20-21 -0.13214603147975623 1106 22-23 -0.04663978725041318 1106 24-25 -0.0366315554219554 1106 26-27 -0.07923579084691568 1106 28-29 -0.03962955247467903 1106 30-31 -0.02687660215521248 1106 32-33 -0.01063658007841184 1106 34-35 -0.0160832170243026 1106 36-37 -0.0014594568331105506 1106 38-39 -0.05996921383957954 1106 40-41 -0.06974491496364976 1106 42-43 -0.061806537079682755 1106 44-45 -0.08987612641234932 1106 46-47 -0.07614827521919665 1106 48-49 -0.09479278364091215 1106 50-51 -0.06924461472060983 1106 52-53 -0.17635064657993382 1106 54-55 -0.1248772091002337 1106 56-57 -0.0841767864819829 1106 58-59 -0.18019449413845479 1106 60-61 -0.1589468503807865 1106 62-63 -0.16005741080120117 1106 64-65 -0.26369642773596524 1106 66-67 -0.2478785800881198 1106 68-69 -0.2032208733565426 1106 70-71 -0.1815361247948033 1106 72-73 -0.21039273190816488 1106 74-75 -0.19521913896551268 1106 76-77 -0.4116914658359505 1106 78-79 -0.2913610932537196 1106 80-81 -0.3347015737364103 1106 82-83 -0.2826020941843055 1106 84-85 -0.24662466126629567 1106 86-87 -0.4509363183058497 1106 88-89 -0.29170885469835994 1106 90-91 -0.2719846446268086 1106 92-93 -0.2987800261228024 1106 94-95 -0.3226392267569871 1106 96-97 -0.4099392079259161 1106 98-99 -0.23635535956260156 1106 100-101 -0.40051827410547247 1107 1 -2.1333356193642032 1107 2 -0.5701570212571561 1107 3 -1.6723222392161325 1107 4 -3.430549206904608 1107 5 -1.8995478837492783 1107 6 -0.346648316107661 1107 7 -0.2918582583472329 1107 8 0.054084176454388455 1107 9 0.2942795739211874 1107 10-11 0.3304994468896183 1107 12-13 0.1616802663627439 1107 14-15 -0.018947633337901948 1107 16-17 -0.05781399556125422 1107 18-19 -0.05555417028951126 1107 20-21 -0.0913621433798042 1107 22-23 -0.09091066598040243 1107 24-25 -0.13540040120756203 1107 26-27 -0.154960094375447 1107 28-29 -0.13290745593842956 1107 30-31 -0.04623033331600368 1107 32-33 -0.0280491388199664 1107 34-35 -0.0020802203787013696 1107 36-37 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-0.03697458363581063 2214 28-29 -0.07604474315224508 2214 30-31 -0.03249106302290983 2214 32-33 -0.010633841800029131 2214 34-35 -0.060787445307525445 2214 36-37 -0.07127924779880601 2214 38-39 -0.09555316588527774 2214 40-41 -0.007628548987661077 2214 42-43 0.0323515611390377 2214 44-45 -0.051653219696596864 2214 46-47 0.038900356604898434 2214 48-49 0.15535159942154309 2214 50-51 0.04221876721162232 2214 52-53 0.05439120046511903 2214 54-55 -0.02342344488184267 2214 56-57 -0.06589717686659213 2214 58-59 0.011159553227699348 2214 60-61 0.07270238143922114 2214 62-63 0.11171417609860157 2214 64-65 0.1495906207060358 2214 66-67 0.11306028706459159 2214 68-69 0.059188784238372705 2214 70-71 0.06433451472913276 2214 72-73 0.08535242111905461 2214 74-75 0.11065797414191536 2214 76-77 -0.02505170454733019 2214 78-79 0.16205632951191262 2214 80-81 0.1254495689618338 2214 82-83 0.08845040858746955 2214 84-85 0.08084014623322844 2214 86-87 0.20177986314929797 2214 88-89 0.18163629120933678 2214 90-91 0.1828686506418471 2214 92-93 0.21727036817461354 2214 94-95 0.22600421057257236 2214 96-97 0.21928761814636744 2214 98-99 0.1792276252840601 2214 100-101 0.2852002252045338 2215 1 -1.287217593541957 2215 2 0.07976457241132451 2215 3 -0.24480695142621656 2215 4 -3.9858763332905838 2215 5 -1.5073942132641065 2215 6 -0.33861860547172995 2215 7 0.028088258001503164 2215 8 0.07083981176226217 2215 9 0.11081315305767703 2215 10-11 0.08595653096273992 2215 12-13 0.07635822008350601 2215 14-15 -0.10565992238368693 2215 16-17 -0.14886003669346337 2215 18-19 -0.19094668781335145 2215 20-21 -0.23271691475387257 2215 22-23 -0.2074649156783579 2215 24-25 -0.17966606985979894 2215 26-27 -0.1314443137748711 2215 28-29 -0.06307600806236024 2215 30-31 -0.07034286013272606 2215 32-33 -0.06304996219449777 2215 34-35 -0.11338305966837936 2215 36-37 -0.06354233381544816 2215 38-39 -0.0017825440725260933 2215 40-41 -0.018228514995023204 2215 42-43 -0.0233998820918373 2215 44-45 -0.03551984312062473 2215 46-47 0.005230145817222365 2215 48-49 0.02188295684471342 2215 50-51 -0.02551051590030795 2215 52-53 -0.19819590409203158 2215 54-55 -0.14245527535226898 2215 56-57 -0.12427223683702238 2215 58-59 -0.114365467109252 2215 60-61 0.023499585440724502 2215 62-63 -0.11619191604346923 2215 64-65 0.012890562635945457 2215 66-67 -0.0065857478055662 2215 68-69 -0.08219576160093567 2215 70-71 -0.11805928761810236 2215 72-73 -0.09856443033432072 2215 74-75 0.058783480236485275 2215 76-77 0.08229644107249356 2215 78-79 -0.05555794701928107 2215 80-81 0.07023171604586764 2215 82-83 0.006730670431643659 2215 84-85 -0.05157758217250574 2215 86-87 0.10654760878816205 2215 88-89 0.16526483812230808 2215 90-91 0.1991429320195941 2215 92-93 -0.0074233313018226 2215 94-95 -0.0379696674358172 2215 96-97 0.057449630988251954 2215 98-99 0.06969199036457852 2215 100-101 0.042097980145943126 2216 1 1.3458508941737009 2216 2 0.6069352821189753 2216 3 0.7615178487480208 2216 4 2.1841714936546524 2216 5 1.4693505121276829 2216 6 1.09062078655613 2216 7 0.9363038450288457 2216 8 0.741048155507066 2216 9 0.7656685244994534 2216 10-11 0.5518092601774711 2216 12-13 0.49274584398868626 2216 14-15 0.42091393205319605 2216 16-17 0.3441122097266671 2216 18-19 0.10785121057921288 2216 20-21 0.09457320253051904 2216 22-23 0.05120763291144925 2216 24-25 0.1708853786962763 2216 26-27 0.12108081270438475 2216 28-29 0.11853007847658859 2216 30-31 0.07088037709971218 2216 32-33 0.07187408633259196 2216 34-35 0.04970091301832724 2216 36-37 -0.0189583970730709 2216 38-39 0.02779827637954213 2216 40-41 0.036882115898087875 2216 42-43 0.009305354949837863 2216 44-45 0.03292442586657529 2216 46-47 0.09218812846009428 2216 48-49 0.08084515395142944 2216 50-51 0.052008628482454355 2216 52-53 -0.050749962552643524 2216 54-55 0.016365506271412755 2216 56-57 0.034120926084725056 2216 58-59 0.09854850203414856 2216 60-61 0.16464099684540656 2216 62-63 0.11649305704576562 2216 64-65 0.05799179626568218 2216 66-67 0.13428069412135102 2216 68-69 0.13347167632146295 2216 70-71 0.11434062662685562 2216 72-73 0.1873559210714646 2216 74-75 0.1708850773268935 2216 76-77 0.15984378394119148 2216 78-79 0.050040239195666913 2216 80-81 0.08364498861210024 2216 82-83 0.16563428225442323 2216 84-85 0.1964827180906923 2216 86-87 0.2074330412464107 2216 88-89 0.2851072163470043 2216 90-91 0.34447757850249516 2216 92-93 0.23398708726719164 2216 94-95 0.18277221966346957 2216 96-97 0.26294695701698245 2216 98-99 0.259769127300018 2216 100-101 0.4418413754457404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 37.0 13 130.0 14 461.0 15 1084.0 16 1933.0 17 3338.0 18 5829.0 19 10871.0 20 11840.0 21 12126.0 22 12084.0 23 12925.0 24 14277.0 25 17049.0 26 21503.0 27 28331.0 28 37621.0 29 51315.0 30 73040.0 31 107347.0 32 164286.0 33 269799.0 34 486216.0 35 966402.0 36 1657820.0 37 1138281.0 38 93362.0 39 115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.58031122780037 41.15301456229597 11.617527865718067 22.64914634418559 2 23.575105915985755 27.871136768752724 31.621528159766005 16.932229155495516 3 23.407105478040997 27.552939071857875 23.58776724409055 25.452188206010582 4 22.842750210509834 27.671743906177817 25.462073417060278 24.02343246625207 5 23.04276990522225 28.473849137606894 25.731777751663788 22.751603205507067 6 23.24873490867359 26.85507460725961 26.9126771084583 22.983513375608506 7 23.567539269231013 28.259960184074128 25.383700934737618 22.78879961195724 8 23.06179123199537 26.356865862334445 27.515243506640253 23.066099399029934 9 22.615242025707833 26.296705386958983 27.654970441935234 23.433082145397947 10-11 23.66628091903204 26.821917107404335 26.34638393807625 23.165418035487374 12-13 23.51046567246737 27.070030986106737 26.099876005567534 23.319627335858357 14-15 23.30324091481619 27.045912943868622 26.2473249456142 23.40352119570099 16-17 23.438928943516277 27.248781452263877 26.147698582940006 23.164591021279847 18-19 23.082322340519447 27.25439745571964 26.64419560078447 23.019084602976445 20-21 22.619617507852308 27.42155049008285 26.87740399086284 23.081428011202004 22-23 22.411633051103514 27.35835121831694 27.389133456436642 22.840882274142903 24-25 22.43442402403188 26.98734879824258 28.072776096289072 22.505451081436473 26-27 22.370561217611098 26.901416252213945 28.53335569477175 22.19466683540321 28-29 22.441059370580643 26.96875059501749 28.497467124742787 22.092722909659084 30-31 22.440011178154823 27.008322263203233 28.419073871024636 22.132592687617308 32-33 22.382091334295108 26.990474142631488 28.531951693907807 22.0954828291656 34-35 22.50288349081543 27.108227502761363 28.42057403633131 21.968314970091896 36-37 22.465254344372948 27.114458958650633 28.237861595133463 22.182425101842952 38-39 22.540378007038083 27.098111003385565 28.261152623164055 22.100358366412298 40-41 22.60693341785547 27.298402304715502 27.906950131235615 22.187714146193414 42-43 22.686201768002512 27.356370230796585 27.67698248287744 22.28044551832346 44-45 22.67534480241765 27.32055859232181 27.74533616877398 22.258760436486558 46-47 22.731783713859237 27.58248168500106 27.505194322374372 22.180540278765335 48-49 22.79601194516242 27.562441015134937 27.30653781657094 22.335009223131703 50-51 22.68343223205172 27.479403018832656 27.516339781287435 22.320824967828184 52-53 22.717185951451956 27.63958413109752 27.389056524882456 22.254173392568067 54-55 22.74248681633572 27.581241163689768 27.298258058051395 22.378013961923113 56-57 22.756247948091204 27.58038530014942 27.358937821417626 22.304428930341746 58-59 22.801926058390663 27.752481186869243 27.15607892946665 22.28951382527344 60-61 22.829179061461964 27.714698177318386 27.06160698092309 22.394515780296558 62-63 22.778394619253238 27.647911971838436 27.20747882410889 22.36621458479944 64-65 22.780625634324707 27.840856309743362 27.07148306919205 22.307034986739886 66-67 22.84302674121591 27.716909959501308 27.00141765621481 22.438645643067975 68-69 22.806724664083177 27.79672644697195 26.941074468398153 22.455474420546725 70-71 22.83069845965719 27.845472202994674 26.92463034869035 22.39919898865779 72-73 22.811177077781842 27.810824154276997 26.8098869356181 22.568111832323062 74-75 22.82846744458572 27.797582310512293 26.912754040012487 22.4611962048895 76-77 22.851181485959895 27.946291004758795 26.789625087533686 22.412902421747628 78-79 22.890608907481536 27.899978209137277 26.69134502705779 22.5180678563234 80-81 22.816773848349058 27.88758261246864 26.766545621277118 22.529097917905187 82-83 22.84319022076856 27.99796015984069 26.649099987363993 22.50974963202676 84-85 22.852412390826913 27.97013978655725 26.658783746747478 22.51866407586836 86-87 22.87637656995665 27.909815831629135 26.760612275160323 22.453195323253887 88-89 22.859316997815334 27.99757550206975 26.652215715308632 22.490891784806287 90-91 22.877309365051186 27.969485868346645 26.629068933942147 22.524135832660022 92-93 22.892436031893514 27.92502904646993 26.696662920741076 22.485872000895483 94-95 22.909014781821153 28.117223301721516 26.525297883786042 22.448464032671293 96-97 22.95786631873089 28.015481321307135 26.497467894058325 22.529184465903647 98-99 22.906254862314633 27.965658523525764 26.642685819033517 22.48540079512608 100-101 22.859586258254996 28.103192909526374 26.480177527254444 22.557043304964182 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8176.0 1 5394.5 2 1812.0 3 867.5 4 678.5 5 584.0 6 519.5 7 487.0 8 453.5 9 414.0 10 388.0 11 377.5 12 396.5 13 484.0 14 572.0 15 764.0 16 2226.5 17 2562.5 18 1706.0 19 2062.0 20 2808.0 21 3872.5 22 5373.0 23 7370.0 24 9728.5 25 13238.5 26 18249.0 27 24134.0 28 31147.5 29 39871.0 30 51067.0 31 64079.0 32 77117.5 33 91225.0 34 105289.5 35 120766.0 36 135131.5 37 147725.0 38 160993.0 39 173015.0 40 180889.0 41 182698.5 42 185188.5 43 192651.0 44 199543.0 45 204967.5 46 210714.5 47 213647.0 48 211997.0 49 208048.5 50 204253.5 51 200767.0 52 198332.0 53 191520.5 54 176358.5 55 159307.5 56 141993.0 57 122109.0 58 104041.0 59 88623.0 60 73093.0 61 57865.5 62 45056.0 63 35134.5 64 26893.5 65 19863.0 66 14559.0 67 10404.5 68 7398.5 69 5634.5 70 4436.0 71 3539.0 72 2813.0 73 2122.5 74 1502.0 75 946.5 76 570.0 77 400.0 78 265.0 79 139.5 80 52.5 81 21.0 82 16.0 83 7.5 84 2.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05229422395967863 2 4.4235643658426205E-4 3 0.014655461072922074 4 0.002788768839335565 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5199427.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.66230538165062 #Duplication Level Percentage of deduplicated Percentage of total 1 81.87031644957615 51.30182771055715 2 10.183499924836875 12.762431642882888 3 3.308482093739888 6.219513459229551 4 1.4763059983062337 3.7003494921051123 5 0.7900550020643521 2.4753333903828523 6 0.5039983159210581 1.8949017830449784 7 0.34271421981634975 1.5032684170536375 8 0.24453133940692773 1.225831797224077 9 0.18193200034600207 1.0260250707938174 >10 1.0030412515286184 11.128483908093484 >50 0.0655908319160872 2.8220373794904634 >100 0.029099056408826766 2.9313761843022124 >500 2.167585055938236E-4 0.09468995185762329 >1k 1.23861501202301E-4 0.21978478146298083 >5k 3.0965375300207475E-5 0.1835000157398799 >10k+ 6.193075060041495E-5 0.510645015779467 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15132 0.29103206949535015 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 11185 0.21511985839978137 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9457 0.1818854269903203 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.923288854714183E-5 0.0 0.0 0.0 0.0 2 1.923288854714183E-5 0.0 0.0 0.0 0.0 3 1.923288854714183E-5 0.0 0.0 0.0 0.0 4 1.923288854714183E-5 1.923288854714183E-5 0.0 1.923288854714183E-5 0.0 5 1.923288854714183E-5 1.923288854714183E-5 0.0 1.923288854714183E-5 0.0 6 1.923288854714183E-5 1.923288854714183E-5 0.0 1.923288854714183E-5 0.0 7 1.923288854714183E-5 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 8 1.923288854714183E-5 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 9 1.923288854714183E-5 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 10-11 1.923288854714183E-5 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 12-13 1.923288854714183E-5 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 14-15 6.73151099149964E-5 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 16-17 1.4424666410356373E-4 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 18-19 2.9810977248069835E-4 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 20-21 5.57753767867113E-4 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 22-23 6.923839876971058E-4 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 24-25 7.693155418856732E-4 3.846577709428366E-5 0.0 1.923288854714183E-5 0.0 26-27 9.424115388099497E-4 6.73151099149964E-5 0.0 1.923288854714183E-5 0.0 28-29 0.0012116719784699354 1.0578088700928005E-4 0.0 1.923288854714183E-5 0.0 30-31 0.001461699529582779 2.692604396599856E-4 0.0 1.923288854714183E-5 0.0 32-33 0.0015867133051392008 4.2312354803712026E-4 0.0 1.923288854714183E-5 0.0 34-35 0.0016540284150541973 4.51972880857833E-4 0.0 1.923288854714183E-5 0.0 36-37 0.0017405764135163355 5.192879907728295E-4 0.0 1.923288854714183E-5 0.0 38-39 0.0020098368531763213 5.385208793199712E-4 0.0 1.923288854714183E-5 9.616444273570915E-6 40-41 0.002346412402751303 5.385208793199712E-4 0.0 1.923288854714183E-5 1.923288854714183E-5 42-43 0.0026445221752320015 5.385208793199712E-4 0.0 1.923288854714183E-5 1.923288854714183E-5 44-45 0.003058029278995551 5.385208793199712E-4 0.0 1.923288854714183E-5 1.923288854714183E-5 46-47 0.0033946048285705328 5.385208793199712E-4 0.0 3.846577709428366E-5 1.923288854714183E-5 48-49 0.0038754270422490782 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 50-51 0.0043466328116540534 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 52-53 0.014395817077535658 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 54-55 0.030878402562436208 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 56-57 0.050553647546162296 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 58-59 0.07334462047452536 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 60-61 0.09770307381948048 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 62-63 0.12672550263711752 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 64-65 0.16079656469837927 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 66-67 0.19763716271042944 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 68-69 0.24090154549722498 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 70-71 0.2896953837413238 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 72-73 0.3457977196333365 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 74-75 0.4066890447735876 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 76-77 0.47831232172314375 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 78-79 0.5556285336826539 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 80-81 0.6430323956851399 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 82-83 0.7380524815522942 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 84-85 0.843544875233367 5.385208793199712E-4 0.0 5.7698665641425486E-5 1.923288854714183E-5 86-87 0.9607404815953758 5.385208793199712E-4 9.616444273570915E-6 7.693155418856732E-5 1.923288854714183E-5 88-89 1.0873698197897577 5.385208793199712E-4 1.923288854714183E-5 7.693155418856732E-5 1.923288854714183E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3515 0.0 65.72476 1 TATCTCG 3275 0.0 62.088078 2 TCGACGG 4280 0.0 56.94418 2 ATATCTC 3745 0.0 52.295403 1 CGACGGC 5175 0.0 48.01345 3 CGGCGCG 5965 0.0 44.354 6 ATCTCGA 6410 0.0 44.19837 1 GACGGCG 6265 0.0 42.005478 4 ACGGCGC 6345 0.0 41.847374 5 TCTCGAG 6445 0.0 39.953247 2 TCGAGGG 7205 0.0 37.382328 4 GGGCGCG 7640 0.0 37.11667 8 CTCGAGG 6995 0.0 36.607716 3 CGAGGGC 7820 0.0 36.20158 5 AGGGCGC 8000 0.0 35.50579 7 GAGGGCG 8185 0.0 34.64525 6 GGCGCGC 13650 0.0 25.12422 7 CGCGCCG 13685 0.0 25.025255 9 GCGCGCC 13830 0.0 24.797224 8 TCGCGAG 195 1.569424E-8 24.363583 2 >>END_MODULE