FastQCFastQC Report
Tue 31 May 2016
SRR2048235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173292
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT561351.0850924324395375No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT79570.15380921857880825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG134900.084.962362
ATATCTC137350.083.5531
GTCGACG121100.083.115231
ATCTCGA147400.077.013143
TCTCGAG130850.072.996624
TCGACGG140200.071.99332
TCGAGGG133250.071.646216
CTCGAGG133400.071.4944465
AGGGCGC136150.070.120159
GAGGGCG138450.069.161118
CGAGGGC138400.069.151787
CGACGGC149700.067.453713
CGGCGCG164000.064.5840766
ACGGCGC158850.063.5383765
GACGGCG160050.063.1510164
CTCGACG30250.059.1943665
TCTCGAC30650.058.576814
GGCGCGC300250.036.5420887
CGCGCCG302650.036.28379
GCGCGCC306450.035.833788
Produced by FastQC (version 0.11.5)