##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048235_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5173292 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14221138107031 31.0 30.0 34.0 28.0 34.0 2 30.666997532712244 31.0 30.0 34.0 27.0 34.0 3 31.365341256592515 31.0 31.0 34.0 28.0 34.0 4 34.241463462723544 37.0 35.0 37.0 30.0 37.0 5 34.237926643228334 35.0 35.0 37.0 30.0 37.0 6 33.971222965956684 36.0 35.0 37.0 29.0 37.0 7 34.06221531666877 35.0 35.0 37.0 29.0 37.0 8 33.79646809033783 35.0 35.0 37.0 28.0 37.0 9 36.019631793449896 39.0 35.0 39.0 31.0 39.0 10-11 35.141373230043854 37.5 34.5 39.0 27.5 39.0 12-13 34.87053514473956 37.0 34.0 39.0 27.0 39.0 14-15 35.837759013023046 39.0 34.0 40.0 27.0 41.0 16-17 35.79088905091767 39.0 34.0 40.5 27.0 41.0 18-19 35.811366147513034 39.0 34.0 40.0 27.0 41.0 20-21 36.158120593231544 39.0 34.5 41.0 28.0 41.0 22-23 35.83504816662195 39.0 34.0 40.5 27.0 41.0 24-25 35.561360831748914 39.0 34.0 40.0 26.0 41.0 26-27 35.14633573747625 38.0 33.0 40.0 25.0 41.0 28-29 34.991082370761205 38.0 33.0 40.0 25.0 41.0 30-31 34.83408466021249 38.0 33.0 40.0 24.5 41.0 32-33 34.79000334796489 38.0 33.0 40.0 25.0 41.0 34-35 34.60593486700538 38.0 33.0 40.0 24.5 41.0 36-37 34.4371462117352 38.0 32.5 40.0 24.0 41.0 38-39 34.67383708478083 38.0 33.0 40.0 24.0 41.0 40-41 34.56387451162625 38.0 32.5 40.0 23.5 41.0 42-43 34.331459639239384 38.0 32.0 40.0 23.0 41.0 44-45 34.09201355732482 38.0 31.5 40.0 23.0 41.0 46-47 34.01422498478725 37.0 31.0 40.0 22.5 41.0 48-49 33.96528709378863 37.0 31.0 40.0 23.0 41.0 50-51 33.90253826770265 37.0 31.0 40.0 23.0 41.0 52-53 33.719612579378854 37.0 31.0 40.0 22.0 41.0 54-55 33.51888565346785 37.0 31.0 40.0 22.0 41.0 56-57 33.869985011478185 37.0 31.0 40.0 23.0 41.0 58-59 33.82789189166202 37.0 31.0 40.0 23.0 41.0 60-61 33.74080672422898 36.5 31.0 40.0 23.0 41.0 62-63 33.56491843104932 36.0 31.0 40.0 23.0 41.0 64-65 33.30938114454007 36.0 31.0 39.5 23.0 41.0 66-67 32.995163717802896 35.0 31.0 39.0 22.0 41.0 68-69 32.757167293089196 35.0 31.0 39.0 22.0 40.5 70-71 32.4917875310344 35.0 31.0 38.5 22.0 40.0 72-73 32.219253137074034 35.0 31.0 37.0 22.0 40.0 74-75 31.911617882771743 35.0 31.0 37.0 21.0 39.0 76-77 31.66873414452538 35.0 30.0 36.5 21.0 39.0 78-79 31.503291231192826 35.0 30.0 36.0 22.0 39.0 80-81 31.29489452750782 34.5 30.0 35.5 22.0 37.0 82-83 31.16406042806012 34.0 30.0 35.0 23.0 37.0 84-85 31.067778795397594 34.0 31.0 35.0 23.0 36.5 86-87 30.92410354953867 34.0 30.0 35.0 23.0 36.0 88-89 30.818350868267245 34.0 30.5 35.0 23.0 36.0 90-91 30.79523270675616 34.0 31.0 35.0 23.5 36.0 92-93 30.77306693687501 34.0 31.0 35.0 24.0 35.5 94-95 30.68517705940434 34.0 31.0 35.0 24.0 35.0 96-97 30.60564626933875 34.0 31.0 35.0 24.0 35.0 98-99 30.482983175896507 34.0 31.0 35.0 23.0 35.0 100-101 29.6676871322941 33.5 30.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29991661198862474 1101 2 0.37844631099180503 1101 3 0.3612869117215993 1101 4 0.08178964119432663 1101 5 0.07348247820613096 1101 6 0.1752120290443102 1101 7 -0.05957234328199945 1101 8 0.2681794309873027 1101 9 0.9655285786033048 1101 10-11 0.546183145104024 1101 12-13 0.22112244604635833 1101 14-15 0.09940131442589006 1101 16-17 -0.003029248614637936 1101 18-19 0.21951514100510394 1101 20-21 1.1083591769130763 1101 22-23 0.6051967017036688 1101 24-25 0.4524053059135582 1101 26-27 1.0034121773164344 1101 28-29 1.0494637688663033 1101 30-31 1.000410435429238 1101 32-33 0.9795089437972138 1101 34-35 0.9200152492106 1101 36-37 1.112459741785635 1101 38-39 1.0644096058106243 1101 40-41 0.8810171023486362 1101 42-43 0.8414830544526737 1101 44-45 0.9722150510522738 1101 46-47 0.9054923690944676 1101 48-49 0.7807706731746649 1101 50-51 0.4926954476262324 1101 52-53 0.46754166357091265 1101 54-55 0.5873637841960253 1101 56-57 0.7524043687645374 1101 58-59 0.7791724895349859 1101 60-61 0.8169698390543481 1101 62-63 0.7555558572133627 1101 64-65 0.750642621974805 1101 66-67 1.435314196282711 1101 68-69 1.3958571701100837 1101 70-71 2.0356292088467 1101 72-73 1.4393785020779895 1101 74-75 0.969899978960747 1101 76-77 1.412877381808137 1101 78-79 1.6437804480902187 1101 80-81 2.2314722844302217 1101 82-83 2.094692208702476 1101 84-85 2.047507431078575 1101 86-87 1.9141281513632578 1101 88-89 1.9092676378647084 1101 90-91 1.8044308780899563 1101 92-93 1.6957564307250266 1101 94-95 1.663957804282198 1101 96-97 1.684339649808006 1101 98-99 1.7383457863602736 1101 100-101 1.9377065414308916 1102 1 1.3928648991966952 1102 2 2.473558974842245 1102 3 1.4500352959706113 1102 4 2.3138416900648835 1102 5 2.182947183067128 1102 6 2.6360305292664847 1102 7 2.427933817654896 1102 8 2.7716936628978672 1102 9 2.25878851868886 1102 10-11 3.057160785116693 1102 12-13 3.2089459994377876 1102 14-15 3.6407625263258296 1102 16-17 3.4739673955241983 1102 18-19 3.530247077263283 1102 20-21 3.5476740595364546 1102 22-23 3.7503947497105514 1102 24-25 3.7575529879459566 1102 26-27 4.442832652211479 1102 28-29 4.438222525127507 1102 30-31 4.394157784394594 1102 32-33 4.3102774740726915 1102 34-35 4.221067379184099 1102 36-37 4.570996318585209 1102 38-39 4.662628877556294 1102 40-41 4.607761642100364 1102 42-43 4.672758331753876 1102 44-45 4.705928206388947 1102 46-47 4.771019624586771 1102 48-49 4.547497150062604 1102 50-51 4.5211940120154 1102 52-53 4.510051714340385 1102 54-55 4.549358579801481 1102 56-57 4.48882727662663 1102 58-59 4.548848288510953 1102 60-61 4.3881624951085705 1102 62-63 4.3273150328361005 1102 64-65 4.317043558772276 1102 66-67 4.248627559548105 1102 68-69 4.150351671722433 1102 70-71 4.080837299851872 1102 72-73 3.911172911457445 1102 74-75 3.902844925163741 1102 76-77 3.7897356443102908 1102 78-79 3.596329632743277 1102 80-81 3.415233578741084 1102 82-83 3.2243079711171347 1102 84-85 3.0217507838571045 1102 86-87 2.90272964099292 1102 88-89 2.8458652894562597 1102 90-91 2.7198852983797543 1102 92-93 2.5876621344092854 1102 94-95 2.560885991122049 1102 96-97 2.574261147290404 1102 98-99 2.5332576134576925 1102 100-101 2.5983207129118924 1103 1 1.249405292007193 1103 2 2.346132068011894 1103 3 1.3369352587951902 1103 4 2.215863351309892 1103 5 2.1082040990366835 1103 6 2.4092163602391494 1103 7 2.281908917129769 1103 8 2.723569263277909 1103 9 2.1232894912607563 1103 10-11 2.969810796234306 1103 12-13 3.189869634889945 1103 14-15 3.38703803627682 1103 16-17 3.2695360035704226 1103 18-19 3.3764588725207005 1103 20-21 3.4129892291485575 1103 22-23 3.58901837591263 1103 24-25 3.5995509615356553 1103 26-27 4.398706251130186 1103 28-29 4.390758124089395 1103 30-31 4.384901481644313 1103 32-33 4.340990360081314 1103 34-35 4.367536418686917 1103 36-37 4.419288233720216 1103 38-39 4.494282660490384 1103 40-41 4.46478797434586 1103 42-43 4.496252508578131 1103 44-45 4.531386348155081 1103 46-47 4.62879601242031 1103 48-49 4.412231617946425 1103 50-51 4.3150823948731585 1103 52-53 4.312003573198702 1103 54-55 4.27626189395767 1103 56-57 4.318053131427746 1103 58-59 4.37710180850339 1103 60-61 4.277795755550077 1103 62-63 4.205702237694236 1103 64-65 4.27237803756946 1103 66-67 4.105212780248685 1103 68-69 4.033857918884671 1103 70-71 3.9902189419664182 1103 72-73 3.799667091199698 1103 74-75 3.7846668619270147 1103 76-77 3.644345866353156 1103 78-79 3.4400417715960856 1103 80-81 3.345458110663497 1103 82-83 3.200945235940317 1103 84-85 3.0640593574591755 1103 86-87 2.9068256481081853 1103 88-89 2.8492852854235657 1103 90-91 2.7037814715880124 1103 92-93 2.5572262037866693 1103 94-95 2.597289593577379 1103 96-97 2.6554189236636496 1103 98-99 2.6790330608174173 1103 100-101 2.7768628129026744 1104 1 -0.2329180576147003 1104 2 -0.2245222216484528 1104 3 -0.14092701239298577 1104 4 -0.5470788276147758 1104 5 -0.40359133883895737 1104 6 -0.9435123387728268 1104 7 -0.5171386421960733 1104 8 -0.4200579310549841 1104 9 0.3068592177615628 1104 10-11 -0.5497298786084883 1104 12-13 -1.0833392150367516 1104 14-15 -1.3038932424283445 1104 16-17 -1.271613779418331 1104 18-19 -1.100622937354359 1104 20-21 -0.5751992689239813 1104 22-23 -1.0829779201206975 1104 24-25 -1.3267616429221505 1104 26-27 -1.2446605175501162 1104 28-29 -1.0710044419252824 1104 30-31 -1.2758745027475094 1104 32-33 -1.0737196857186504 1104 34-35 -1.074177795914217 1104 36-37 -1.230641294583922 1104 38-39 -1.0968109508500277 1104 40-41 -1.0512236688567853 1104 42-43 -0.996756559841792 1104 44-45 -1.19263749941371 1104 46-47 -1.3684925636444376 1104 48-49 -1.5370958512614656 1104 50-51 -1.6723198632514311 1104 52-53 -1.442776837019121 1104 54-55 -1.452696078280109 1104 56-57 -1.1589961079601352 1104 58-59 -0.7966218707827082 1104 60-61 -0.8743912835415877 1104 62-63 -0.9615486378933618 1104 64-65 -0.7643846279815207 1104 66-67 -0.5651918336787887 1104 68-69 -0.672924721941488 1104 70-71 -0.7758463817123342 1104 72-73 -0.8389058989855762 1104 74-75 -0.6952509719685835 1104 76-77 -0.6007561876556764 1104 78-79 -0.6716003748576398 1104 80-81 -0.5730045778250634 1104 82-83 -0.6759930674834678 1104 84-85 -0.4482490932385126 1104 86-87 -0.47259177260119856 1104 88-89 -0.4755952184910832 1104 90-91 -0.23118123769257792 1104 92-93 -0.03873860316601352 1104 94-95 0.03934619920396898 1104 96-97 0.2345760735546918 1104 98-99 0.3537843140899497 1104 100-101 0.48314806257508636 1105 1 -0.909691335832747 1105 2 -1.5863929964690122 1105 3 -0.9226684069915905 1105 4 -1.693781095073426 1105 5 -1.7130896790461705 1105 6 -1.8511091405844944 1105 7 -1.518969737618292 1105 8 -1.8761996506920653 1105 9 -1.4697883037526367 1105 10-11 -2.3245588000956374 1105 12-13 -2.6113491594376654 1105 14-15 -2.7482270187207547 1105 16-17 -2.935082376893579 1105 18-19 -2.4721489171998883 1105 20-21 -2.435664846468761 1105 22-23 -2.9665969961388186 1105 24-25 -2.88385449504635 1105 26-27 -3.877641581328085 1105 28-29 -3.3899401307485206 1105 30-31 -3.333500716346286 1105 32-33 -3.2154738645860306 1105 34-35 -3.430194544021173 1105 36-37 -3.442742717113614 1105 38-39 -3.4596879785245314 1105 40-41 -3.4854963228910236 1105 42-43 -3.514549167571875 1105 44-45 -3.555820378618776 1105 46-47 -3.997470266878892 1105 48-49 -3.826299311275939 1105 50-51 -3.5771242591929777 1105 52-53 -3.471836438559869 1105 54-55 -3.345967781140846 1105 56-57 -2.9285595021536146 1105 58-59 -2.978024158228127 1105 60-61 -2.9589376760873094 1105 62-63 -3.1549134131370913 1105 64-65 -2.9194964868176 1105 66-67 -2.8555848025770594 1105 68-69 -2.654437909314936 1105 70-71 -2.6427295385466216 1105 72-73 -2.5718710571362635 1105 74-75 -2.3578661031919133 1105 76-77 -2.541758014825234 1105 78-79 -2.6816158222715956 1105 80-81 -2.602491073628162 1105 82-83 -2.7301329046958607 1105 84-85 -2.6424400440879836 1105 86-87 -2.6018710792248356 1105 88-89 -2.65374561230551 1105 90-91 -2.571151613205423 1105 92-93 -2.6844977392921052 1105 94-95 -2.660321338321914 1105 96-97 -2.5582670035598554 1105 98-99 -2.608503264924746 1105 100-101 -2.6646998114235174 1106 1 0.9663123154712032 1106 2 1.9683130542501068 1106 3 0.9761175538612257 1106 4 1.8217257369176494 1106 5 1.6248405041229432 1106 6 2.064355447785964 1106 7 1.9302537880522692 1106 8 2.386614058517523 1106 9 1.9175631959725408 1106 10-11 2.5656756785670964 1106 12-13 2.610165637121085 1106 14-15 2.5970586841639474 1106 16-17 2.4934329458199898 1106 18-19 2.6797763199708626 1106 20-21 2.9415216466495835 1106 22-23 2.806875297656063 1106 24-25 2.8624168923193736 1106 26-27 3.6589775985632187 1106 28-29 3.8640844937875443 1106 30-31 3.7641300746139024 1106 32-33 3.7405709941742487 1106 34-35 3.865417688562353 1106 36-37 4.066681687498111 1106 38-39 4.068059192895156 1106 40-41 3.8375288721485035 1106 42-43 4.02429168546314 1106 44-45 4.00035457291704 1106 46-47 4.02653368444264 1106 48-49 3.847584188502779 1106 50-51 3.856311208873805 1106 52-53 3.916705791725498 1106 54-55 3.9354948005043475 1106 56-57 3.930918483596166 1106 58-59 4.001097100889282 1106 60-61 3.9429893544446877 1106 62-63 3.9427644139589724 1106 64-65 3.925196487181317 1106 66-67 3.959864008301814 1106 68-69 3.7331739088028755 1106 70-71 3.6919562344171126 1106 72-73 3.4913964797653385 1106 74-75 3.5252610651274594 1106 76-77 3.4748488884608904 1106 78-79 3.2702157207149725 1106 80-81 3.033140143038178 1106 82-83 2.922054858081374 1106 84-85 2.7846149409811325 1106 86-87 2.6950127537818567 1106 88-89 2.6739967155060107 1106 90-91 2.4700163176531937 1106 92-93 2.4315333264253383 1106 94-95 2.436801149682232 1106 96-97 2.458414096854785 1106 98-99 2.4592718727118985 1106 100-101 2.4373155996297022 1107 1 -0.9041363293794618 1107 2 -2.4296126976187686 1107 3 -1.014924753520969 1107 4 -2.1037022322588967 1107 5 -2.1255698993694594 1107 6 -2.0557936013633977 1107 7 -2.460916374473502 1107 8 -2.146676426064989 1107 9 -0.38126721742575853 1107 10-11 -1.7337087737867023 1107 12-13 -2.2729696935169237 1107 14-15 -2.889045650240604 1107 16-17 -3.0147964332186774 1107 18-19 -2.84368751775677 1107 20-21 -1.8440721886591689 1107 22-23 -2.656916701606754 1107 24-25 -2.898826067008862 1107 26-27 -3.1419320159132127 1107 28-29 -3.093594417024857 1107 30-31 -3.216884588412917 1107 32-33 -3.2399634633628693 1107 34-35 -3.562231159013585 1107 36-37 -3.1764938426399922 1107 38-39 -3.3742754881005013 1107 40-41 -3.8277232098170018 1107 42-43 -3.97609752402348 1107 44-45 -4.259128938376833 1107 46-47 -4.442030076111166 1107 48-49 -4.186085369302717 1107 50-51 -4.112576221007444 1107 52-53 -4.537350326315902 1107 54-55 -4.500940344686104 1107 56-57 -4.26221442816075 1107 58-59 -4.332290714861095 1107 60-61 -3.8476468112832194 1107 62-63 -3.7298114640372724 1107 64-65 -3.9199917823972186 1107 66-67 -3.8607441675162946 1107 68-69 -3.662940331495683 1107 70-71 -3.7458646992709888 1107 72-73 -3.822988490920128 1107 74-75 -3.7961378544389888 1107 76-77 -3.7294045411669465 1107 78-79 -3.5431179581728074 1107 80-81 -3.282947489568336 1107 82-83 -3.253787430871249 1107 84-85 -3.1381598611889494 1107 86-87 -3.066637729418435 1107 88-89 -2.6218566311887628 1107 90-91 -2.3772148697870534 1107 92-93 -2.0968622642999826 1107 94-95 -1.3428567514200864 1107 96-97 -0.850682739018378 1107 98-99 -0.31441519318533295 1107 100-101 0.015621461384885293 1108 1 -0.22231642063128731 1108 2 -0.9061311043439773 1108 3 -0.45364746100816333 1108 4 -0.9557636339786555 1108 5 -0.1412265221194815 1108 6 -1.1411797900553964 1108 7 -0.3435046399312469 1108 8 -1.2121602763560517 1108 9 0.10702838566569284 1108 10-11 -0.45982699715362685 1108 12-13 0.3061502255257267 1108 14-15 -1.4215115473462987 1108 16-17 -0.19788910879298527 1108 18-19 -1.209942269242191 1108 20-21 -0.1212986614011271 1108 22-23 -0.8129550747680696 1108 24-25 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1.5333724560476192 2216 90-91 1.413744747208316 2216 92-93 1.3855249084625036 2216 94-95 1.4007037239181912 2216 96-97 1.3614245645246932 2216 98-99 1.363397313966253 2216 100-101 1.453153122887155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 111.0 11 1520.0 12 6756.0 13 14343.0 14 19330.0 15 21206.0 16 21735.0 17 22524.0 18 24988.0 19 37842.0 20 44609.0 21 54387.0 22 74418.0 23 96461.0 24 117404.0 25 134979.0 26 145667.0 27 149626.0 28 151635.0 29 154176.0 30 156099.0 31 163104.0 32 185107.0 33 219800.0 34 280000.0 35 395587.0 36 643639.0 37 957385.0 38 757128.0 39 121608.0 40 118.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.646095631939843 42.59429411183627 11.682183641517382 21.0774266147065 2 22.849415232092902 25.226763210705315 33.94035500734886 17.983466549852917 3 21.366104986921286 29.423392300299305 24.144625897784238 25.06587681499517 4 23.27196489505999 23.157395614279427 27.694605888053037 25.876033602607546 5 22.51914254985027 26.196104917333102 25.036398486688938 26.248354046127687 6 22.001387124484758 22.22629227192279 28.253556922748608 27.518763680843843 7 24.215296565513796 24.76353548185565 24.89911646201297 26.122051490617583 8 25.211026170569916 21.994853567129017 27.173722264275824 25.62039799802524 9 21.361001853365323 26.556011143388 27.101137921462776 24.9818490817839 10-11 25.05766154317212 22.076561694178483 25.967614045369952 26.898162717279444 12-13 24.109126258482995 22.381338613787893 25.70636840139702 27.80316672633209 14-15 23.937997313896066 22.389379915148808 25.83239067116258 27.84023209979255 16-17 24.07598488544625 23.502839197942045 25.702734351743533 26.718441564868172 18-19 22.940953265348256 22.58034149241914 26.961014379238595 27.51769086299401 20-21 22.670891289074607 25.904530969390205 26.055686031248378 25.368891710286807 22-23 21.57125340681097 23.58204269670002 28.32678631526632 26.519917581222685 24-25 21.570742575520576 22.685178025906907 29.46721352670601 26.2768658718665 26-27 21.55275596274094 22.833758465595988 30.780961136545166 24.832524435117907 28-29 21.64396481002812 22.684008557800333 30.91582690480259 24.756199727368955 30-31 21.62763091663877 22.71029742763409 30.879206122523144 24.782865533204003 32-33 21.549740474730598 22.854509275718442 30.99508011533082 24.600670134220145 34-35 21.676777572191945 22.986562521504684 30.880665541399942 24.45599436490343 36-37 21.661223964333708 22.92172792235808 30.69330375575456 24.72374435755365 38-39 21.739716219382167 22.91595950895484 30.59953893961524 24.744785332047755 40-41 22.048156183722085 23.211796279815637 29.602253265425578 25.1377942710367 42-43 22.116226959545298 23.27510219798148 29.370022028526517 25.238648813946707 44-45 22.210974100547382 23.398329175459196 29.15805054424509 25.232646179748336 46-47 22.21743581456325 23.756450052133935 28.942607052404945 25.08350708089787 48-49 22.252183943706758 23.84716235918595 28.695442483884705 25.20521121322259 50-51 22.202651585076634 23.925165122418395 28.719896050535027 25.152287241969944 52-53 22.250106895183958 24.208965973697214 28.586507392198236 24.954419738920596 54-55 22.28091258771741 24.115374184585473 28.41181886416939 25.191894363527727 56-57 22.287292557097597 24.395640995258145 28.479936659275495 24.83712978836876 58-59 22.419980934621098 24.702819880301586 28.22951870791513 24.647680477162186 60-61 22.371596267908327 24.854811984322556 28.057733837564165 24.715857910204953 62-63 22.396640969226862 24.85435733397664 28.16960542232604 24.57939627447046 64-65 22.413523149282895 25.120097609027287 28.049122299688477 24.41725694200134 66-67 22.48122119937056 24.942399949425052 27.93246001974086 24.643918831463534 68-69 22.534140183693804 24.985697701357953 27.786734870516717 24.693427244431525 70-71 22.578690633478086 25.024032211765686 27.740923768645143 24.656353386111093 72-73 22.5350333479953 25.020560094772154 27.593411065907414 24.850995491325133 74-75 22.56524472231608 25.07936919083632 27.6248373376179 24.730548749229698 76-77 22.606059200238146 25.261004119235537 27.563088479478242 24.569848201048075 78-79 22.659761198455566 25.304470084471365 27.457093805752102 24.578674911320963 80-81 22.605586539480083 25.364013861966423 27.51669536534957 24.513704233203924 82-83 22.686788998220763 25.594338844880255 27.388873677207958 24.32999847969102 84-85 22.688294549832978 25.696766333075022 27.387923857366616 24.227015259725384 86-87 22.731144611866593 25.685642129211733 27.51107929319107 24.072133965730604 88-89 22.702830285789013 25.794226459398278 27.44243562297613 24.06050763183658 90-91 22.74877513093428 25.983278067797805 27.433258988719956 23.834687812547966 92-93 22.763056754909684 25.9513010193455 27.580705474418938 23.704936751325878 94-95 22.839532065577625 26.223056550677914 27.408028045601164 23.529383338143298 96-97 22.892357635381615 26.23120037415374 27.303577260398615 23.572864730066033 98-99 22.92270760890755 26.279842105665026 27.401999127620364 23.39545115780706 100-101 23.016687639780486 26.45853992333699 27.098152239677265 23.42662019720526 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5854.0 1 3912.5 2 1565.0 3 939.5 4 619.5 5 460.5 6 374.5 7 321.0 8 307.5 9 297.5 10 291.5 11 284.0 12 258.5 13 343.5 14 502.5 15 796.0 16 6397.5 17 7268.5 18 2868.0 19 3076.5 20 3635.0 21 4683.0 22 6085.5 23 7913.0 24 9997.5 25 12996.0 26 17208.0 27 22099.5 28 27946.0 29 35001.5 30 43980.0 31 54450.5 32 64944.0 33 75259.5 34 85845.5 35 98853.5 36 111055.0 37 121354.0 38 131455.0 39 141672.5 40 150782.0 41 154608.5 42 158420.0 43 166556.5 44 173958.5 45 180433.0 46 188145.5 47 193164.5 48 193342.0 49 192691.5 50 192213.0 51 192115.5 52 193740.0 53 191515.0 54 180492.5 55 166569.0 56 153812.5 57 138040.0 58 122303.5 59 108948.5 60 95727.0 61 82601.5 62 70552.5 63 60733.0 64 52160.0 65 44452.0 66 38221.5 67 33142.5 68 28894.0 69 25400.0 70 22738.0 71 20170.0 72 17565.0 73 15272.5 74 13099.0 75 10880.0 76 8898.5 77 7296.5 78 5896.5 79 4662.5 80 3540.5 81 2692.0 82 1986.5 83 1434.5 84 1056.0 85 742.5 86 491.5 87 316.5 88 207.5 89 129.5 90 66.5 91 41.0 92 28.5 93 19.0 94 14.0 95 10.0 96 8.5 97 7.0 98 6.0 99 5.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006862168228663682 2 0.007442069769114134 3 0.0 4 5.7990154045045205E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0024645815469144213 22-23 0.0038466802183213323 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.73202053933936E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.011143774602322853 46-47 0.0015077440051711752 48-49 4.735862580345358E-4 50-51 1.7397046213513562E-4 52-53 0.0 54-55 5.7990154045045205E-5 56-57 1.93300513483484E-5 58-59 7.828670796081103E-4 60-61 0.0 62-63 2.7062071887687763E-4 64-65 0.0 66-67 0.014893804563902442 68-69 0.003334433857590099 70-71 5.219113864054069E-4 72-73 8.215271823048071E-4 74-75 0.0 76-77 3.86601026966968E-5 78-79 9.6650256741742E-6 80-81 0.0 82-83 2.8995077022522603E-5 84-85 1.93300513483484E-5 86-87 0.015560691335420464 88-89 0.003721034884557067 90-91 0.0016623844159579628 92-93 4.155961039894907E-4 94-95 5.7990154045045205E-5 96-97 3.38275898596097E-4 98-99 0.0016527193902837883 100-101 3.86601026966968E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5173292.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.61616428881747 #Duplication Level Percentage of deduplicated Percentage of total 1 87.34752663598282 61.681472911484235 2 7.343982689385939 10.372077762558183 3 2.208142536973957 4.677916684922374 4 0.9935470429313087 2.8064192484922432 5 0.5392544660368723 1.9040040983569158 6 0.32935222088598376 1.395455432338292 7 0.22518586042410965 1.1131233200659354 8 0.1641614432646769 0.9273961157974251 9 0.1275873454511685 0.810875605279844 >10 0.6525171897122011 8.121272731236058 >50 0.04841708282622655 2.360421426298697 >100 0.019693200953712256 2.043508737015808 >500 2.474168993612372E-4 0.10867346156271397 >1k 3.298870899650681E-4 0.43317723670935043 >5k 2.7490590830068683E-5 0.15446765584248845 >10k+ 2.7490590830068683E-5 1.0897375720394733 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 56135 1.0850924324395375 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7957 0.15380921857880825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.86601026966968E-5 0.0 0.0 0.0 0.0 2 1.1598030809009041E-4 0.0 0.0 0.0 0.0 3 1.3531035943843881E-4 0.0 0.0 0.0 0.0 4 1.7397046213513562E-4 0.0 0.0 0.0 1.93300513483484E-5 5 2.7062071887687763E-4 0.0 0.0 0.0 1.93300513483484E-5 6 2.89950770225226E-4 0.0 0.0 0.0 1.93300513483484E-5 7 2.89950770225226E-4 0.0 0.0 0.0 1.93300513483484E-5 8 3.4794092427027125E-4 0.0 0.0 0.0 3.86601026966968E-5 9 4.2526112966366486E-4 0.0 0.0 1.93300513483484E-5 3.86601026966968E-5 10-11 5.509064634279295E-4 0.0 0.0 2.89950770225226E-5 6.765517971921941E-5 12-13 7.442069769114135E-4 0.0 0.0 3.86601026966968E-5 9.6650256741742E-5 14-15 8.988473876982007E-4 0.0 0.0 3.86601026966968E-5 1.3531035943843881E-4 16-17 0.0010341577471366394 0.0 0.0 3.86601026966968E-5 1.546404107867872E-4 18-19 0.001585064210564569 0.0 0.0 5.7990154045045205E-5 1.546404107867872E-4 20-21 0.0026965421630946017 0.0 0.0 5.7990154045045205E-5 1.546404107867872E-4 22-23 0.0036340496534895 0.0 0.0 5.7990154045045205E-5 1.546404107867872E-4 24-25 0.004194621142591603 0.0 0.0 6.765517971921941E-5 2.0296553915765824E-4 26-27 0.004813182785738752 9.6650256741742E-6 0.0 7.73202053933936E-5 2.5129066752852924E-4 28-29 0.006272601662539056 1.93300513483484E-5 0.0 7.73202053933936E-5 2.89950770225226E-4 30-31 0.007616040231249271 1.93300513483484E-5 0.0 7.73202053933936E-5 2.89950770225226E-4 32-33 0.007905991001474495 1.93300513483484E-5 0.0 7.73202053933936E-5 2.9961579589940024E-4 34-35 0.008060631412261283 1.93300513483484E-5 0.0 7.73202053933936E-5 3.092808215735744E-4 36-37 0.008273261977093115 1.93300513483484E-5 0.0 7.73202053933936E-5 3.2861087292192286E-4 38-39 0.00883383346619522 1.93300513483484E-5 0.0 7.73202053933936E-5 3.5760594994444547E-4 40-41 0.009442730083668194 1.93300513483484E-5 0.0 7.73202053933936E-5 3.769360012927938E-4 42-43 0.009655360648500026 2.89950770225226E-5 0.0 7.73202053933936E-5 3.86601026966968E-4 44-45 0.009810001059286814 3.86601026966968E-5 0.0 7.73202053933936E-5 3.86601026966968E-4 46-47 0.010196602086253781 3.86601026966968E-5 0.0 1.1598030809009041E-4 3.86601026966968E-4 48-49 0.010795833678052584 3.86601026966968E-5 0.0 1.3531035943843881E-4 3.86601026966968E-4 50-51 0.011482050500918951 3.86601026966968E-5 0.0 1.44975385112613E-4 3.86601026966968E-4 52-53 0.019591007041551103 3.86601026966968E-5 0.0 1.546404107867872E-4 3.9626605264114225E-4 54-55 0.03225219067471931 4.8325128370871E-5 0.0 1.546404107867872E-4 4.2526112966366486E-4 56-57 0.04692369964811574 5.7990154045045205E-5 0.0 1.546404107867872E-4 4.3492615533783903E-4 58-59 0.06388581970629147 5.7990154045045205E-5 0.0 1.7397046213513562E-4 4.4459118101201325E-4 60-61 0.08374744746671944 5.7990154045045205E-5 0.0 1.7397046213513562E-4 4.4459118101201325E-4 62-63 0.10548409020793723 5.7990154045045205E-5 0.0 1.7397046213513562E-4 4.735862580345358E-4 64-65 0.1299172751122496 5.7990154045045205E-5 0.0 1.7397046213513562E-4 4.929163093828843E-4 66-67 0.15796517961870316 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.025813350570585E-4 68-69 0.1916574591188744 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.025813350570585E-4 70-71 0.22880981781040002 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.025813350570585E-4 72-73 0.26927728030816744 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.025813350570585E-4 74-75 0.3130115214838056 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.31576412079581E-4 76-77 0.36340496534894995 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.412414377537553E-4 78-79 0.42061225231438704 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.412414377537553E-4 80-81 0.4834349191965194 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.605714891021037E-4 82-83 0.5537383159504625 7.73202053933936E-5 0.0 1.7397046213513562E-4 5.605714891021037E-4 84-85 0.6310101962154853 7.73202053933936E-5 0.0 1.93300513483484E-4 5.605714891021037E-4 86-87 0.7185559987721551 7.73202053933936E-5 0.0 1.93300513483484E-4 5.605714891021037E-4 88-89 0.8136791814573776 7.73202053933936E-5 0.0 2.0296553915765824E-4 5.605714891021037E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 13490 0.0 84.96236 2 ATATCTC 13735 0.0 83.553 1 GTCGACG 12110 0.0 83.11523 1 ATCTCGA 14740 0.0 77.01314 3 TCTCGAG 13085 0.0 72.99662 4 TCGACGG 14020 0.0 71.9933 2 TCGAGGG 13325 0.0 71.64621 6 CTCGAGG 13340 0.0 71.494446 5 AGGGCGC 13615 0.0 70.12015 9 GAGGGCG 13845 0.0 69.16111 8 CGAGGGC 13840 0.0 69.15178 7 CGACGGC 14970 0.0 67.45371 3 CGGCGCG 16400 0.0 64.584076 6 ACGGCGC 15885 0.0 63.538376 5 GACGGCG 16005 0.0 63.151016 4 CTCGACG 3025 0.0 59.194366 5 TCTCGAC 3065 0.0 58.57681 4 GGCGCGC 30025 0.0 36.542088 7 CGCGCCG 30265 0.0 36.2837 9 GCGCGCC 30645 0.0 35.83378 8 >>END_MODULE