FastQCFastQC Report
Tue 31 May 2016
SRR2048235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173292
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT188990.36531864043243645No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT104080.20118717443361017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98240.1898984244461747No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52380.10125080896264893No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51930.10038095665197325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG37800.060.990441
TCGACGG42150.058.271612
TATCTCG31300.055.8557052
ATATCTC32900.051.1468851
ATCTCGA65500.050.7279931
CGACGGC51100.048.4374053
TCTCGAG71550.044.752192
CGGCGCG60100.043.4687086
ACGGCGC61600.042.2559975
GACGGCG62350.041.5203634
TCGAGGG81150.040.9742134
CTCGAGG78550.040.703613
GGGCGCG85200.040.6421288
CGAGGGC86450.039.889645
GAGGGCG89700.038.8679926
AGGGCGC89750.038.6346447
GGCGCGC146550.025.8321769
CGCGCCG145850.024.1975219
GCGCGCC148250.023.773758
GGTCGAC5300.022.4220831
Produced by FastQC (version 0.11.5)