FastQCFastQC Report
Tue 31 May 2016
SRR1567939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9896580
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670280.6772844760513228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG29200.076.777971
TCGACGG35950.062.494212
CGACGGC61950.036.419153
ATCTCGA29950.030.6082151
ACGGCGC77300.029.6787175
GACGGCG77900.029.2672084
CGGCGCG83050.027.5095146
GGGCGCG31750.026.3297338
CGCGCCG109900.025.8025689
GGCGCGC113100.025.7439617
GCGCGCC113300.025.5308288
TCTCGAG35500.025.1539972
TCGAGGG37200.023.366074
AGGGCGC39100.022.2306357
CTCGAGG43550.020.2862743
CGCCGGA111650.019.6973849
CGAGGGC45700.018.9161475
GCGCCGG119450.018.450558
GAGGGCG53400.016.633286
CCGGATC119650.016.25651610-11
Produced by FastQC (version 0.11.5)