##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567939_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9896580 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.813401397250363 33.0 31.0 34.0 30.0 34.0 2 32.026300196633585 34.0 31.0 34.0 30.0 34.0 3 32.04098526965881 34.0 31.0 34.0 30.0 34.0 4 35.44588029400055 37.0 35.0 37.0 33.0 37.0 5 35.37596563661386 37.0 35.0 37.0 33.0 37.0 6 35.363964824212 37.0 35.0 37.0 32.0 37.0 7 35.289026714279075 37.0 35.0 37.0 32.0 37.0 8 35.2407915663795 37.0 35.0 37.0 32.0 37.0 9 36.80905130863389 39.0 37.0 39.0 32.0 39.0 10-11 36.792203316701325 39.0 37.0 39.0 32.0 39.0 12-13 36.724468654828236 39.0 37.0 39.0 32.0 39.0 14-15 37.96233845429431 40.0 38.0 41.0 32.0 41.0 16-17 37.994328950000906 40.0 38.0 41.0 32.0 41.0 18-19 38.04994927540625 40.0 38.0 41.0 32.0 41.0 20-21 38.06526840585334 40.0 38.0 41.0 32.0 41.0 22-23 38.079462854844806 40.0 38.0 41.0 32.0 41.0 24-25 38.08745627277302 40.0 38.0 41.0 32.5 41.0 26-27 38.07828103243747 40.0 38.0 41.0 33.0 41.0 28-29 37.986399089382395 40.0 38.0 41.0 32.0 41.0 30-31 37.982720343795535 40.0 38.0 41.0 32.5 41.0 32-33 37.97675444446465 40.0 38.0 41.0 32.5 41.0 34-35 37.89280903099859 40.0 37.5 41.0 32.0 41.0 36-37 37.872943127827995 40.0 37.0 41.0 32.0 41.0 38-39 37.85801771925251 40.0 37.5 41.0 32.0 41.0 40-41 37.787458647330695 40.0 37.0 41.0 31.5 41.0 42-43 37.68035018157788 40.0 37.0 41.0 31.0 41.0 44-45 37.616178063533056 40.0 37.0 41.0 31.0 41.0 46-47 37.56377036309513 40.0 37.0 41.0 31.0 41.0 48-49 37.49192756487595 40.0 36.0 41.0 31.0 41.0 50-51 37.29539052884936 40.0 36.0 41.0 30.0 41.0 52-53 37.23923067362665 39.5 36.0 41.0 30.5 41.0 54-55 37.399401560943275 39.0 36.0 41.0 31.0 41.0 56-57 37.45943694690489 40.0 36.0 41.0 31.0 41.0 58-59 37.3311879962573 39.0 36.0 41.0 31.0 41.0 60-61 37.157014089715844 39.0 35.0 41.0 31.0 41.0 62-63 36.913591159774384 39.0 35.0 41.0 31.0 41.0 64-65 36.61878603517579 38.5 35.0 40.0 31.0 41.0 66-67 36.33318762643256 37.5 35.0 40.0 30.5 41.0 68-69 35.97367777555479 37.0 35.0 40.0 30.0 41.0 70-71 35.62316032407155 37.0 35.0 39.0 30.0 41.0 72-73 35.23563276404576 36.0 34.0 39.0 30.0 40.5 74-75 34.86136907901518 36.0 34.0 38.5 30.0 40.0 76-77 34.50219196934699 35.0 34.0 37.0 29.5 39.0 78-79 34.16445767123592 35.0 34.0 37.0 29.0 39.0 80-81 33.85173433650817 35.0 34.0 36.5 29.0 38.5 82-83 33.5647793480172 35.0 34.0 36.0 29.0 37.0 84-85 33.31252291195544 35.0 34.0 36.0 29.0 37.0 86-87 33.139094313389066 35.0 34.0 35.0 29.0 36.5 88-89 32.97956142424959 35.0 33.5 35.0 29.0 36.0 90-91 32.826587821247344 35.0 33.0 35.0 29.0 36.0 92-93 32.67200729949134 35.0 33.0 35.0 29.0 36.0 94-95 32.57445748935491 35.0 33.0 35.0 29.0 35.5 96-97 32.46101249118382 35.0 33.0 35.0 28.0 35.0 98-99 32.33159505606987 35.0 33.0 35.0 28.0 35.0 100-101 31.923909572801918 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3252799154093111 1101 2 0.3867152577965811 1101 3 0.39742775971173216 1101 4 0.34156056620025765 1101 5 0.3582242422317563 1101 6 0.44580334633683805 1101 7 0.38624468952475866 1101 8 0.39462969800386816 1101 9 0.5264703483438069 1101 10-11 0.4892901729074808 1101 12-13 0.4620261312150049 1101 14-15 0.5772293536458477 1101 16-17 0.533313265682267 1101 18-19 0.5072303273920866 1101 20-21 0.5345135780885926 1101 22-23 0.5371880372545093 1101 24-25 0.4775387668895732 1101 26-27 0.5239357408942098 1101 28-29 0.5254818538572223 1101 30-31 0.47631874866218027 1101 32-33 0.43353167914030877 1101 34-35 0.556654474384878 1101 36-37 0.5356827193184088 1101 38-39 0.4960917829407805 1101 40-41 0.49720681597406013 1101 42-43 0.5093587664010357 1101 44-45 0.4422967072075039 1101 46-47 0.42053746714850604 1101 48-49 0.40040496025451233 1101 50-51 0.52772509113273 1101 52-53 0.48056778749065643 1101 54-55 0.4563342278647582 1101 56-57 0.4834941325104438 1101 58-59 0.4820136620374953 1101 60-61 0.4364626391288553 1101 62-63 0.449142025638281 1101 64-65 0.4668518121276364 1101 66-67 0.4563836792043503 1101 68-69 0.4662237401925964 1101 70-71 0.45294843401718055 1101 72-73 0.45415650066865965 1101 74-75 0.42054719962795417 1101 76-77 0.4354542367206591 1101 78-79 0.4088186525988817 1101 80-81 0.3584280934442816 1101 82-83 0.39046944908145775 1101 84-85 0.35658444478618634 1101 86-87 0.35287672625889854 1101 88-89 0.36766524552720625 1101 90-91 0.3255705420484034 1101 92-93 0.38447067581903127 1101 94-95 0.3886642182311846 1101 96-97 0.38896462395224063 1101 98-99 0.41757738097798835 1101 100-101 0.42410920824363174 1102 1 0.2482589712681289 1102 2 0.30965784785578876 1102 3 0.34220255648027376 1102 4 0.3125546450356609 1102 5 0.2703038199449921 1102 6 0.30211060977433135 1102 7 0.2738424175203633 1102 8 0.29407848337864806 1102 9 0.3350990080110634 1102 10-11 0.378306630896887 1102 12-13 0.38562234927145767 1102 14-15 0.5228631931536611 1102 16-17 0.4493560143768818 1102 18-19 0.4597520547862928 1102 20-21 0.45403981049021525 1102 22-23 0.4326529054908548 1102 24-25 0.3875000657052041 1102 26-27 0.3870406605766519 1102 28-29 0.4111672560751458 1102 30-31 0.43285722432328555 1102 32-33 0.40697646910593477 1102 34-35 0.428100562411899 1102 36-37 0.4369572062095344 1102 38-39 0.4442533762019778 1102 40-41 0.4063889504613556 1102 42-43 0.42944330661885033 1102 44-45 0.37535136174503947 1102 46-47 0.3506261332762932 1102 48-49 0.3314678298931355 1102 50-51 0.4766889468712634 1102 52-53 0.3234908768569227 1102 54-55 0.33618177760020274 1102 56-57 0.36009462335231746 1102 58-59 0.3909056654136478 1102 60-61 0.33329301485709095 1102 62-63 0.32105775039171647 1102 64-65 0.349197398148263 1102 66-67 0.3250573616546717 1102 68-69 0.3791578898755503 1102 70-71 0.3534650287099197 1102 72-73 0.4240517923677629 1102 74-75 0.4138539043433056 1102 76-77 0.42885563144197647 1102 78-79 0.40646482220132185 1102 80-81 0.39053369192947684 1102 82-83 0.4358157402034806 1102 84-85 0.45268467090490816 1102 86-87 0.4538595239682337 1102 88-89 0.454847077828191 1102 90-91 0.43571779460917526 1102 92-93 0.4350670025434624 1102 94-95 0.44775732188307416 1102 96-97 0.44361380003555695 1102 98-99 0.43510570784334135 1102 100-101 0.41442256651601284 1103 1 0.1680501664319749 1103 2 0.25035010854136175 1103 3 0.2784655669722653 1103 4 0.25734210798722756 1103 5 0.22957188833106557 1103 6 0.21534800037074575 1103 7 0.22381616265106885 1103 8 0.24606127340139494 1103 9 0.314245694901345 1103 10-11 0.2886187334162784 1103 12-13 0.3263765845859439 1103 14-15 0.4122651136008031 1103 16-17 0.4235285878915178 1103 18-19 0.3984801367476649 1103 20-21 0.3729540751050564 1103 22-23 0.35762928805156946 1103 24-25 0.4087261820347763 1103 26-27 0.4206481572475198 1103 28-29 0.41622066154467774 1103 30-31 0.38254839619813197 1103 32-33 0.4080293709756333 1103 34-35 0.37911461941622093 1103 36-37 0.4318741022828405 1103 38-39 0.3417717337100896 1103 40-41 0.35045737037206237 1103 42-43 0.3130381237205455 1103 44-45 0.3186216356197491 1103 46-47 0.28834177054905297 1103 48-49 0.2851074235895723 1103 50-51 0.42050970712788427 1103 52-53 0.2984880204704581 1103 54-55 0.2964378869828863 1103 56-57 0.2781766145821152 1103 58-59 0.31118552161897384 1103 60-61 0.3124393733126922 1103 62-63 0.29533231780342817 1103 64-65 0.3096568882185906 1103 66-67 0.22438797196592475 1103 68-69 0.21507772593687946 1103 70-71 0.2405341572433244 1103 72-73 0.278524744278954 1103 74-75 0.268936301637801 1103 76-77 0.2645013539286083 1103 78-79 0.3113710988866387 1103 80-81 0.2893772435623987 1103 82-83 0.264613898182553 1103 84-85 0.2716871849230955 1103 86-87 0.2754968604226917 1103 88-89 0.296925721014766 1103 90-91 0.30384870270120956 1103 92-93 0.2936381325379074 1103 94-95 0.3190662137046445 1103 96-97 0.2673391848515223 1103 98-99 0.28608511001259984 1103 100-101 0.21014383893168187 1104 1 0.2231631447905542 1104 2 0.2641479410046088 1104 3 0.28858486392357463 1104 4 0.30145262189687827 1104 5 0.2871506979704108 1104 6 0.2754709534299593 1104 7 0.2691261328120689 1104 8 0.28970545153460847 1104 9 0.30109824822143594 1104 10-11 0.33331337353256885 1104 12-13 0.3860615081724035 1104 14-15 0.513482441588458 1104 16-17 0.4763724229487849 1104 18-19 0.4281629955580044 1104 20-21 0.47040538565543244 1104 22-23 0.4440079365860399 1104 24-25 0.45106482906901846 1104 26-27 0.3750973867286902 1104 28-29 0.4206622619998157 1104 30-31 0.40896478676601333 1104 32-33 0.41967309077694637 1104 34-35 0.4129294680314075 1104 36-37 0.4773723506864087 1104 38-39 0.393584390884115 1104 40-41 0.40158640128760226 1104 42-43 0.34233552190782746 1104 44-45 0.3326850844208309 1104 46-47 0.31273834843598536 1104 48-49 0.33700859822541673 1104 50-51 0.4224424314365507 1104 52-53 0.31667874627851944 1104 54-55 0.35075270530433045 1104 56-57 0.372666598253403 1104 58-59 0.36726036086878366 1104 60-61 0.3546589828454927 1104 62-63 0.29992876416886816 1104 64-65 0.33381825666081255 1104 66-67 0.27677332017054823 1104 68-69 0.3022441274898071 1104 70-71 0.3083565970837725 1104 72-73 0.3413922551930213 1104 74-75 0.3617907796487998 1104 76-77 0.3164466483219357 1104 78-79 0.3090984554228271 1104 80-81 0.2847317285097688 1104 82-83 0.3001046482197083 1104 84-85 0.3529400631487931 1104 86-87 0.35642670045970704 1104 88-89 0.3239748661889905 1104 90-91 0.33034258632587665 1104 92-93 0.318226680687026 1104 94-95 0.3373309496249348 1104 96-97 0.3366996423404274 1104 98-99 0.349045403519753 1104 100-101 0.373525430495274 1105 1 0.0841823636784369 1105 2 0.1783011502440388 1105 3 0.20425870181851735 1105 4 0.19643551386700153 1105 5 0.18256799323746264 1105 6 0.18950485606166012 1105 7 0.15146986240405624 1105 8 0.18746467506048958 1105 9 0.22890628972977112 1105 10-11 0.2542453477863873 1105 12-13 0.3455026406586512 1105 14-15 0.3738846770370401 1105 16-17 0.3693625291313225 1105 18-19 0.3870627937667308 1105 20-21 0.35790927850128185 1105 22-23 0.3752746499644246 1105 24-25 0.3819461635153516 1105 26-27 0.3735329835308008 1105 28-29 0.3570434905470776 1105 30-31 0.3133207342953028 1105 32-33 0.32671587892841103 1105 34-35 0.3475687215495853 1105 36-37 0.3116115540678024 1105 38-39 0.3001032555309564 1105 40-41 0.2578869738256415 1105 42-43 0.20191025164321275 1105 44-45 0.2327341331807986 1105 46-47 0.21703013688902217 1105 48-49 0.2280658695442952 1105 50-51 0.33003612405778426 1105 52-53 0.24098254833562294 1105 54-55 0.20452725206681066 1105 56-57 0.17598995789976613 1105 58-59 0.15740783453075124 1105 60-61 0.21939223160593713 1105 62-63 0.2124037134648944 1105 64-65 0.17699037701972742 1105 66-67 0.21006091515708647 1105 68-69 0.21962961732191388 1105 70-71 0.2668735778444926 1105 72-73 0.237575534205817 1105 74-75 0.24107676817013157 1105 76-77 0.22519722666844189 1105 78-79 0.2108780501140899 1105 80-81 0.21186373827306682 1105 82-83 0.20510000559088581 1105 84-85 0.1807898397702985 1105 86-87 0.25090441654882056 1105 88-89 0.24599974517594347 1105 90-91 0.23239053184247638 1105 92-93 0.1985939277274298 1105 94-95 0.20092660185464695 1105 96-97 0.22537048876355215 1105 98-99 0.217500398318478 1105 100-101 0.21184102103858393 1106 1 0.05686319014744612 1106 2 0.1121455445678734 1106 3 0.1502841739434757 1106 4 0.16434558508632335 1106 5 0.08856639604705663 1106 6 0.1406190190401233 1106 7 0.15361158671164787 1106 8 0.20492245307156054 1106 9 0.1749909290296756 1106 10-11 0.22561522003587697 1106 12-13 0.24159093154890598 1106 14-15 0.2852215575480699 1106 16-17 0.2916853238227546 1106 18-19 0.17432724003824518 1106 20-21 0.1967885074755884 1106 22-23 0.20230037922219424 1106 24-25 0.220401199410297 1106 26-27 0.22744953289963377 1106 28-29 0.23571996686889207 1106 30-31 0.23596313823613002 1106 32-33 0.2272768069496678 1106 34-35 0.18577848278312104 1106 36-37 0.18226878612808406 1106 38-39 0.20521560089228785 1106 40-41 0.12673003113487624 1106 42-43 0.09282218973810075 1106 44-45 0.13315365241469834 1106 46-47 0.11908515494442895 1106 48-49 0.128412960814849 1106 50-51 0.19780861875774747 1106 52-53 0.15841907294971946 1106 54-55 0.13764714328745242 1106 56-57 0.12493296037808932 1106 58-59 0.1402368394933049 1106 60-61 0.14075475788264669 1106 62-63 0.08556126741667214 1106 64-65 0.19326034213752763 1106 66-67 0.09440730806097974 1106 68-69 0.14030371896887317 1106 70-71 0.12254961369321649 1106 72-73 0.1243367789645049 1106 74-75 0.14411166767437322 1106 76-77 0.1522188241136888 1106 78-79 0.1554184660872835 1106 80-81 0.16547696790884459 1106 82-83 0.15030704600923883 1106 84-85 0.14945038412733425 1106 86-87 0.15586088464694825 1106 88-89 0.14427877156305868 1106 90-91 0.15107651371042863 1106 92-93 0.13841863332270776 1106 94-95 0.18065788379298198 1106 96-97 0.20334176120775993 1106 98-99 0.16028089038169213 1106 100-101 0.11387852762618778 1107 1 0.08651228150082702 1107 2 0.09018203394928292 1107 3 0.14061484555836046 1107 4 0.148223715159034 1107 5 0.12046037817973598 1107 6 0.09713250009970409 1107 7 0.10691123719630724 1107 8 0.07008859276356105 1107 9 0.1287144091939254 1107 10-11 0.060311582665740104 1107 12-13 0.08064164744685343 1107 14-15 0.1718932525634429 1107 16-17 0.14977057730418863 1107 18-19 0.18234628229701144 1107 20-21 0.13237641260090527 1107 22-23 0.2011390708433396 1107 24-25 0.15280591081280193 1107 26-27 0.15358600128237043 1107 28-29 0.11753534899609974 1107 30-31 0.16952556826346665 1107 32-33 0.1337568472929931 1107 34-35 0.15079653180928432 1107 36-37 0.17022058008332408 1107 38-39 0.13934559765544918 1107 40-41 0.12643479911410083 1107 42-43 0.16284069083457098 1107 44-45 0.14848753920057334 1107 46-47 0.11008765223806449 1107 48-49 0.03696969681291051 1107 50-51 0.1532550247768114 1107 52-53 0.03086159814171907 1107 54-55 0.07733747623227316 1107 56-57 0.0791198034209657 1107 58-59 0.08756047680615353 1107 60-61 0.03608514158273124 1107 62-63 0.08203347566955443 1107 64-65 0.09904509027708741 1107 66-67 0.06716816272431458 1107 68-69 0.1089556640573548 1107 70-71 0.09794127146476228 1107 72-73 0.12500921058638426 1107 74-75 0.144078744764073 1107 76-77 0.0929174923388274 1107 78-79 0.14900883633616502 1107 80-81 0.1513725764663505 1107 82-83 0.1196216358198896 1107 84-85 0.14963418687807462 1107 86-87 0.14966350025006392 1107 88-89 0.0939734184557608 1107 90-91 0.17448519870439583 1107 92-93 0.17276082769913614 1107 94-95 0.18247826550189927 1107 96-97 0.18380620046173846 1107 98-99 0.11338095605123755 1107 100-101 0.10949858703211746 1108 1 -0.05081204740363532 1108 2 0.05780631197108477 1108 3 0.05135718005912793 1108 4 0.054173054039964086 1108 5 0.03676704800439268 1108 6 0.05753275768108068 1108 7 0.05703009631827172 1108 8 0.02211046936486838 1108 9 0.08278906698949129 1108 10-11 0.07537064836860452 1108 12-13 0.05898744678207635 1108 14-15 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-0.450862829600581 2214 74-75 -0.3535095539356661 2214 76-77 -0.3944654266870202 2214 78-79 -0.45477717978566545 2214 80-81 -0.38701398368884554 2214 82-83 -0.4705530694269129 2214 84-85 -0.4139768880014003 2214 86-87 -0.4930513523091804 2214 88-89 -0.40596149147601324 2214 90-91 -0.4175455349873971 2214 92-93 -0.3361869494346621 2214 94-95 -0.30868693721110674 2214 96-97 -0.3677867912148045 2214 98-99 -0.4329348953442569 2214 100-101 -0.4357608218484934 2215 1 -0.3051562001766044 2215 2 -0.33215608391103757 2215 3 -0.3398943491674693 2215 4 -0.3485945603442673 2215 5 -0.30469875314175 2215 6 -0.3254561530034863 2215 7 -0.41362060102638054 2215 8 -0.45165024187232206 2215 9 -0.5031510549823679 2215 10-11 -0.4906926599653332 2215 12-13 -0.4821326510117103 2215 14-15 -0.6066398440736194 2215 16-17 -0.5905311966022779 2215 18-19 -0.6508494647712197 2215 20-21 -0.6838150887572354 2215 22-23 -0.5822001084678305 2215 24-25 -0.49777321498450533 2215 26-27 -0.5665647017853601 2215 28-29 -0.6209401347984809 2215 30-31 -0.6110721581933518 2215 32-33 -0.6159006358390684 2215 34-35 -0.6189832808194069 2215 36-37 -0.6530813613649826 2215 38-39 -0.6467454533923771 2215 40-41 -0.5656471162534089 2215 42-43 -0.4689397894734242 2215 44-45 -0.45173575222013085 2215 46-47 -0.524557499758032 2215 48-49 -0.49073998933582885 2215 50-51 -0.37964845087213206 2215 52-53 -0.5250167492468947 2215 54-55 -0.38679780349963266 2215 56-57 -0.3531069572698371 2215 58-59 -0.4321619464417523 2215 60-61 -0.4189221009400228 2215 62-63 -0.39147204529616886 2215 64-65 -0.48851355597856383 2215 66-67 -0.4084100471443861 2215 68-69 -0.4501268084249048 2215 70-71 -0.42279962085372347 2215 72-73 -0.41750789216440864 2215 74-75 -0.4290612880308373 2215 76-77 -0.4180055433465313 2215 78-79 -0.35328041758819495 2215 80-81 -0.37327866233408713 2215 82-83 -0.29984444326950666 2215 84-85 -0.34949670339426575 2215 86-87 -0.339507218454699 2215 88-89 -0.35406463369718466 2215 90-91 -0.3802511264426158 2215 92-93 -0.35441354171979356 2215 94-95 -0.3784115448343215 2215 96-97 -0.3379134603206069 2215 98-99 -0.36918901195284803 2215 100-101 -0.292203476569032 2216 1 -0.2937379723347959 2216 2 -0.37205022409825617 2216 3 -0.33743812964108955 2216 4 -0.31885139123156137 2216 5 -0.26475980623845174 2216 6 -0.2394079702413734 2216 7 -0.31886983065159313 2216 8 -0.34881324036143724 2216 9 -0.40895283606587896 2216 10-11 -0.4994049854012488 2216 12-13 -0.5258004140999901 2216 14-15 -0.6879095417249559 2216 16-17 -0.7143580697395606 2216 18-19 -0.6522257453801004 2216 20-21 -0.5823275586095207 2216 22-23 -0.6406189839639893 2216 24-25 -0.6752956658701166 2216 26-27 -0.6036672896831661 2216 28-29 -0.6239292216963506 2216 30-31 -0.6804034961766874 2216 32-33 -0.6672638839945151 2216 34-35 -0.7464774102326501 2216 36-37 -0.8069848556415593 2216 38-39 -0.7802931022130934 2216 40-41 -0.6929173632452716 2216 42-43 -0.7423097044354279 2216 44-45 -0.6868636075798307 2216 46-47 -0.6202954546928225 2216 48-49 -0.6731531394247128 2216 50-51 -0.6530844971101928 2216 52-53 -0.6583950792793374 2216 54-55 -0.595010422034477 2216 56-57 -0.6193274753814819 2216 58-59 -0.5089019524103477 2216 60-61 -0.509140418990981 2216 62-63 -0.5038913369601659 2216 64-65 -0.6196299376493783 2216 66-67 -0.5860018402418916 2216 68-69 -0.514147430385016 2216 70-71 -0.4567125863408492 2216 72-73 -0.4669025009223162 2216 74-75 -0.47726657761260327 2216 76-77 -0.44741922079901286 2216 78-79 -0.47670137465545537 2216 80-81 -0.5184430235201916 2216 82-83 -0.44117444585995713 2216 84-85 -0.4755270852245559 2216 86-87 -0.4406272189679399 2216 88-89 -0.4565178281337765 2216 90-91 -0.47878456380135503 2216 92-93 -0.5424570057364235 2216 94-95 -0.5563442413025541 2216 96-97 -0.5462007625497947 2216 98-99 -0.6288130156311205 2216 100-101 -0.5965359334155345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 148.0 11 1600.0 12 5847.0 13 11589.0 14 15857.0 15 18747.0 16 19936.0 17 20693.0 18 21995.0 19 24003.0 20 26476.0 21 39988.0 22 60332.0 23 74926.0 24 81285.0 25 82161.0 26 82140.0 27 88730.0 28 106572.0 29 129599.0 30 146213.0 31 171038.0 32 223642.0 33 301170.0 34 419790.0 35 647617.0 36 1037370.0 37 2184671.0 38 3128336.0 39 723776.0 40 331.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.412930527515567 29.317390452055154 26.154277538301113 24.115401482128167 2 23.44515984309731 28.93805738952244 26.77303674602742 20.84374602135283 3 22.089388455405807 27.396868413128576 26.975318746476056 23.53842438498956 4 23.069019802800565 26.81985089798698 26.74021732760206 23.370911971610393 5 23.79644281155712 27.395261797509846 26.820719885051197 21.98757550588183 6 23.644693419342843 27.114578975767383 25.80234788179351 23.43837972309626 7 24.02135889367842 27.58291248087723 26.05318200833015 22.342546617114195 8 23.138205319413373 27.502379609925853 26.44463036725818 22.914784703402592 9 22.562077000337492 27.12437023699096 26.825822657928295 23.487730104743253 10-11 22.93241705720562 27.492184168672413 26.273020578826223 23.30237819529575 12-13 22.93110852435892 27.733722154522066 26.24028199640684 23.094887324712175 14-15 22.716617411725334 28.019902856261158 26.419776589003614 22.843703143009897 16-17 22.57037279545055 27.94670987351186 26.541087931386397 22.94182939965119 18-19 22.403784943889708 27.840956168696664 26.74370338035968 23.011555507053952 20-21 22.373269351634605 27.928663235178213 26.79626194099376 22.901805472193423 22-23 22.440631105173704 27.689199157829897 27.18550472332576 22.68466501367064 24-25 22.438716202971126 27.461466486402376 27.442187098977627 22.657630211648872 26-27 22.430253683595748 27.491396017614168 27.505633259166295 22.57271703962379 28-29 22.42760125214973 27.57032732519719 27.46885287644823 22.53321854620485 30-31 22.4231502195708 27.489875290251785 27.515116333117096 22.57185815706032 32-33 22.45179647918776 27.52795410131581 27.506502246230518 22.513747173265916 34-35 22.479639430995356 27.549168500633552 27.48670247701731 22.484489591353782 36-37 22.495056492072933 27.541387969392005 27.410246514308422 22.553309024226635 38-39 22.535047174000933 27.603719799641226 27.257115609268663 22.604117417089185 40-41 22.583794599210268 27.67364354989882 27.17183848662687 22.57072336426404 42-43 22.592262031824834 27.692753861898034 27.110843780733063 22.604140325544066 44-45 22.561310107287067 27.741503009185138 27.12357448815661 22.573612395371185 46-47 22.5700246733088 27.80535480626294 27.099047616545434 22.525572903882825 48-49 22.566678616927714 27.745333335488958 27.08846978995564 22.599518257627686 50-51 22.640946531009604 27.72689910937476 27.117318394545453 22.514835965070187 52-53 22.588747310607175 27.78540052412023 27.11039841969656 22.515453745576036 54-55 22.59023651919603 27.731819778624534 27.096387305659103 22.581556396520334 56-57 22.54519331624896 27.76145085036293 27.12538694599042 22.567968887397686 58-59 22.573262682664115 27.818064422254963 27.11733750447124 22.491335390609684 60-61 22.53053074900622 27.801265689763532 27.081926281604353 22.58627727962589 62-63 22.581497850772692 27.781925675334307 27.07798552631313 22.55859094757987 64-65 22.537856512047597 27.870213750608798 27.086155015166856 22.505774722176753 66-67 22.55424601225878 27.81217349831962 27.066062215431998 22.567518273989602 68-69 22.577264064959817 27.856107867566372 27.060332963508603 22.506295103965208 70-71 22.57118848846657 27.90805287362431 27.0472857337874 22.473472904121717 72-73 22.589205802921065 27.852934868579432 27.018089857922963 22.53976947057654 74-75 22.54783896102636 27.85723206584607 27.078146333222048 22.516782639905518 76-77 22.574840231104346 27.915813922563594 27.02954949041488 22.479796355917184 78-79 22.569502949035574 27.875154959452754 27.041621261490317 22.51372083002136 80-81 22.552984667804107 27.899387611472548 27.05924168101939 22.488386039703954 82-83 22.56197466923417 27.956844958264547 27.048812090492973 22.4323682820083 84-85 22.537042403695207 27.932174175586994 27.026964897474826 22.503818523242973 86-87 22.53387133248764 27.983613673709552 27.049806485616724 22.43270850818609 88-89 22.54154836178334 28.022553673775548 27.014790327092637 22.421107637348477 90-91 22.566710231074595 28.02024219170131 26.974014339271683 22.43903323795241 92-93 22.547930203753257 28.027117049954036 27.034703308557766 22.390249437734944 94-95 22.515030444860752 28.12135606441821 26.995492382216884 22.36812110850415 96-97 22.531152175802145 28.109180141018413 26.966295427309234 22.393372255870208 98-99 22.49164357788246 28.14736504934028 26.996709974556865 22.364281398220395 100-101 22.523856726263013 28.23738604649283 26.89660973790946 22.3421474893347 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 62592.0 1 33459.0 2 3320.0 3 2031.0 4 1509.5 5 1143.0 6 878.0 7 672.0 8 562.5 9 487.0 10 460.0 11 446.5 12 478.0 13 755.0 14 1148.5 15 1812.5 16 5747.5 17 5699.5 18 2427.5 19 3087.5 20 4406.0 21 6711.5 22 9696.5 23 13607.5 24 18759.0 25 25376.5 26 34076.0 27 44305.0 28 55954.5 29 69345.5 30 85430.5 31 104395.0 32 123870.0 33 144652.5 34 166338.5 35 191064.5 36 221983.5 37 254437.0 38 288221.0 39 321273.5 40 350344.0 41 375300.0 42 398854.0 43 420491.0 44 434417.5 45 442381.5 46 446509.5 47 444694.5 48 436217.0 49 421462.0 50 402843.5 51 384627.0 52 367031.0 53 344956.0 54 316066.0 55 282232.0 56 245887.0 57 211624.5 58 181415.5 59 152025.0 60 122668.5 61 97509.0 62 77498.0 63 60567.5 64 46764.0 65 35872.0 66 27128.0 67 20436.5 68 15456.0 69 12101.5 70 9755.0 71 7961.0 72 6388.0 73 5059.5 74 3892.0 75 2651.0 76 1698.0 77 1125.5 78 681.0 79 336.5 80 140.0 81 59.5 82 23.5 83 13.5 84 12.0 85 5.5 86 2.5 87 1.0 88 1.5 89 3.0 90 2.5 91 2.0 92 1.5 93 0.5 94 0.5 95 1.5 96 1.5 97 1.0 98 1.5 99 3.0 100 26.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011215995828862092 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.547025336025172E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.4651526082747778E-4 38-39 0.0013438985993141066 40-41 0.008134123101111697 42-43 0.0038144490318877834 44-45 4.749115350959625E-4 46-47 0.0046581748442391215 48-49 4.041800298689042E-5 50-51 0.001066024828779235 52-53 0.007669316066762457 54-55 0.004107479553542739 56-57 1.1620175858730996E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 1.0104500746722604E-5 72-73 1.2630625933403257E-4 74-75 4.445980328557947E-4 76-77 9.902410731788152E-4 78-79 3.991277794955429E-4 80-81 8.588825634714215E-4 82-83 7.780465574976405E-4 84-85 8.487780627246988E-4 86-87 0.0015965111179821717 88-89 1.0609725784058735E-4 90-91 0.0012327490911001578 92-93 2.020900149344521E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9896580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.03708748538732 #Duplication Level Percentage of deduplicated Percentage of total 1 82.71373281648368 56.276014758780526 2 9.848070757955577 13.400681034426162 3 3.1596582792903907 6.44921840316026 4 1.428273061883453 3.88702157057546 5 0.769886553745309 2.619041940549647 6 0.46900921531989004 1.9146012608503336 7 0.3241953070500245 1.5440113127680166 8 0.22899756865406826 1.2464262089966265 9 0.1659051838927375 1.0158934959710508 >10 0.8740691604542707 9.973385318105736 >50 0.01674453410325684 0.7329072595198414 >100 0.0013238671259907585 0.1254990695545337 >500 5.944152475392832E-5 0.02864294804853658 >1k 5.9402013797864664E-5 0.10941458981607044 >5k 0.0 0.0 >10k+ 1.4850502780072779E-5 0.6772408288772834 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67028 0.6772844760513228 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0209001493445212E-5 0.0 0.0 0.0 0.0 2 4.0418002986890423E-5 0.0 0.0 0.0 0.0 3 5.0522503733613024E-5 0.0 0.0 0.0 0.0 4 7.073150522705824E-5 0.0 0.0 0.0 0.0 5 1.0104500746722605E-4 0.0 0.0 0.0 0.0 6 1.4146301045411648E-4 0.0 0.0 0.0 0.0 7 1.717765126942843E-4 0.0 0.0 0.0 0.0 8 1.8188101344100688E-4 0.0 0.0 1.0104500746722606E-5 0.0 9 2.121945156811747E-4 0.0 0.0 1.0104500746722606E-5 0.0 10-11 2.6271701941478774E-4 0.0 0.0 1.0104500746722606E-5 0.0 12-13 3.940755291221816E-4 1.0104500746722606E-5 0.0 1.0104500746722606E-5 0.0 14-15 5.254340388295755E-4 1.0104500746722606E-5 0.0 1.0104500746722606E-5 0.0 16-17 6.365835470435242E-4 1.5156751120083908E-5 0.0 1.0104500746722606E-5 0.0 18-19 7.376285545107502E-4 2.0209001493445212E-5 0.0 2.0209001493445212E-5 0.0 20-21 8.639348138447828E-4 2.0209001493445212E-5 0.0 2.0209001493445212E-5 0.0 22-23 9.700320716853701E-4 2.0209001493445212E-5 0.0 2.0209001493445212E-5 1.0104500746722606E-5 24-25 0.0011317040836329316 2.0209001493445212E-5 0.0 2.0209001493445212E-5 1.0104500746722606E-5 26-27 0.0013792643519276357 2.0209001493445212E-5 0.0 2.0209001493445212E-5 1.0104500746722606E-5 28-29 0.0016722948735825912 3.0313502240167816E-5 0.0 2.0209001493445212E-5 1.0104500746722606E-5 30-31 0.0019350118929973787 3.0313502240167816E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 32-33 0.002137101907931831 3.0313502240167816E-5 0.0 3.536575261352912E-5 2.0209001493445212E-5 34-35 0.0029808277202831683 3.536575261352912E-5 0.0 4.0418002986890423E-5 2.0209001493445212E-5 36-37 0.007037784770092294 4.0418002986890423E-5 0.0 4.0418002986890423E-5 3.0313502240167816E-5 38-39 0.007270188287266914 4.0418002986890423E-5 0.0 5.0522503733613024E-5 3.0313502240167816E-5 40-41 0.007361128793987417 4.0418002986890423E-5 0.0 6.062700448033563E-5 3.0313502240167816E-5 42-43 0.007482382802948089 4.0418002986890423E-5 0.0 6.062700448033563E-5 3.0313502240167816E-5 44-45 0.007684472817882541 4.0418002986890423E-5 0.0 6.062700448033563E-5 5.0522503733613024E-5 46-47 0.007861301580950187 4.0418002986890423E-5 0.0 6.062700448033563E-5 6.062700448033563E-5 48-49 0.007987607840284218 5.0522503733613024E-5 0.0 6.062700448033563E-5 6.062700448033563E-5 50-51 0.00814422760185842 5.0522503733613024E-5 0.0 6.062700448033563E-5 7.073150522705824E-5 52-53 0.008316004114552704 5.0522503733613024E-5 0.0 6.062700448033563E-5 7.073150522705824E-5 54-55 0.008452414874633458 5.0522503733613024E-5 0.0 7.073150522705824E-5 7.073150522705824E-5 56-57 0.008588825634714215 5.557475410697433E-5 0.0 7.073150522705824E-5 7.073150522705824E-5 58-59 0.008785863399275305 6.062700448033563E-5 0.0 7.073150522705824E-5 7.073150522705824E-5 60-61 0.008987953414209757 6.062700448033563E-5 0.0 7.578375560041954E-5 7.073150522705824E-5 62-63 0.009245618183251182 7.073150522705824E-5 0.0 8.083600597378085E-5 7.073150522705824E-5 64-65 0.00957906670789303 7.073150522705824E-5 0.0 9.094050672050344E-5 7.578375560041954E-5 66-67 0.009963037736268488 7.073150522705824E-5 0.0 9.094050672050344E-5 8.588825634714214E-5 68-69 0.010306590761657058 7.073150522705824E-5 0.0 9.094050672050344E-5 9.599275709386474E-5 70-71 0.010892651804966968 7.073150522705824E-5 0.0 9.094050672050344E-5 1.0104500746722605E-4 72-73 0.011786900121051918 7.578375560041954E-5 0.0 9.094050672050344E-5 1.0104500746722605E-4 74-75 0.012802402446097542 8.083600597378085E-5 0.0 9.094050672050344E-5 1.0609725784058735E-4 76-77 0.013969472282344002 8.083600597378085E-5 0.0 9.094050672050344E-5 1.1114950821394866E-4 78-79 0.015454833892112224 8.083600597378085E-5 0.0 1.0104500746722605E-4 1.1114950821394866E-4 80-81 0.017591935800044055 8.083600597378085E-5 0.0 1.0104500746722605E-4 1.1114950821394866E-4 82-83 0.020496979764726805 9.094050672050344E-5 0.0 1.0104500746722605E-4 1.1114950821394866E-4 84-85 0.024680243073869963 9.599275709386474E-5 0.0 1.0609725784058735E-4 1.2125400896067126E-4 86-87 0.030515592255102267 1.1620175858730996E-4 0.0 1.1114950821394866E-4 1.2630625933403257E-4 88-89 0.03844762534127951 1.2630625933403257E-4 0.0 1.2125400896067126E-4 1.3135850970739387E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2920 0.0 76.77797 1 TCGACGG 3595 0.0 62.49421 2 CGACGGC 6195 0.0 36.41915 3 ATCTCGA 2995 0.0 30.608215 1 ACGGCGC 7730 0.0 29.678717 5 GACGGCG 7790 0.0 29.267208 4 CGGCGCG 8305 0.0 27.509514 6 GGGCGCG 3175 0.0 26.329733 8 CGCGCCG 10990 0.0 25.802568 9 GGCGCGC 11310 0.0 25.743961 7 GCGCGCC 11330 0.0 25.530828 8 TCTCGAG 3550 0.0 25.153997 2 TCGAGGG 3720 0.0 23.36607 4 AGGGCGC 3910 0.0 22.230635 7 CTCGAGG 4355 0.0 20.286274 3 CGCCGGA 11165 0.0 19.697384 9 CGAGGGC 4570 0.0 18.916147 5 GCGCCGG 11945 0.0 18.45055 8 GAGGGCG 5340 0.0 16.63328 6 CCGGATC 11965 0.0 16.256516 10-11 >>END_MODULE