FastQCFastQC Report
Tue 31 May 2016
SRR1567939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9896580
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT2242772.266207113972706No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT313810.31708933793290206No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229310.23170630662309608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139120.14057381438840488No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT116420.11763659769334457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG569350.093.648361
TCGACGG582550.091.4918752
TATCTCG414700.089.305382
CGACGGC614650.086.620993
ATATCTC430600.086.4547651
ACGGCGC643400.083.953725
GACGGCG639350.083.9060444
CGGCGCG648450.083.18276
ATCTCGA478950.077.285613
TCTCGAG483500.073.463764
TCGAGGG529550.066.797256
CTCGAGG529300.066.783925
CGAGGGC544500.064.9632347
AGGGCGC567900.063.4574179
GAGGGCG571550.062.794548
TACGGCG7650.050.2930264
CGCGCCG1221100.047.693469
GGCGCGC1231300.047.6378447
GCGCGCC1234700.047.522058
ATCCATA710750.038.41017516-17
Produced by FastQC (version 0.11.5)