##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567939_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9896580 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.691718755368015 34.0 31.0 34.0 31.0 34.0 2 32.90985704152344 34.0 31.0 34.0 31.0 34.0 3 32.97068795482884 34.0 31.0 34.0 31.0 34.0 4 36.41756071289274 37.0 37.0 37.0 35.0 37.0 5 36.290105874958826 37.0 37.0 37.0 35.0 37.0 6 36.292430112220586 37.0 37.0 37.0 35.0 37.0 7 36.243946595692655 37.0 37.0 37.0 35.0 37.0 8 36.24894236190684 37.0 37.0 37.0 35.0 37.0 9 38.02478310689147 39.0 38.0 39.0 35.0 39.0 10-11 38.05743322440682 39.0 38.5 39.0 35.0 39.0 12-13 38.053203278304224 39.0 38.0 39.0 35.0 39.0 14-15 39.58328720628742 41.0 39.5 41.0 37.0 41.0 16-17 39.567089792635436 41.0 39.5 41.0 37.0 41.0 18-19 39.610651709984666 41.0 39.5 41.0 37.0 41.0 20-21 39.658862556559946 41.0 40.0 41.0 37.0 41.0 22-23 39.65520220116444 41.0 40.0 41.0 37.0 41.0 24-25 39.62960765234051 41.0 40.0 41.0 37.0 41.0 26-27 39.57507159038779 41.0 40.0 41.0 37.0 41.0 28-29 39.4189256288536 41.0 39.0 41.0 36.5 41.0 30-31 39.047619329101565 40.0 38.0 41.0 35.5 41.0 32-33 38.78156752130534 40.0 38.0 41.0 35.0 41.0 34-35 38.67569069314854 40.0 37.0 41.0 35.0 41.0 36-37 38.627214603428655 40.0 37.0 41.0 35.0 41.0 38-39 38.56514376683663 40.0 38.0 41.0 35.0 41.0 40-41 38.181952452261285 40.0 37.0 41.0 34.0 41.0 42-43 38.15750183396688 40.0 37.0 41.0 34.0 41.0 44-45 38.091484836175724 40.0 37.0 41.0 33.0 41.0 46-47 37.99168429902047 40.0 37.0 41.0 33.0 41.0 48-49 37.77825905514834 40.0 37.0 41.0 33.0 41.0 50-51 37.750816595227846 40.0 37.0 41.0 33.0 41.0 52-53 37.616962779061055 40.0 36.0 41.0 32.5 41.0 54-55 37.507074059927774 40.0 36.0 41.0 33.0 41.0 56-57 37.30428168114642 40.0 36.0 41.0 32.0 41.0 58-59 37.04085729615686 39.5 35.0 41.0 32.0 41.0 60-61 36.74728411228929 39.0 35.0 41.0 31.5 41.0 62-63 36.42356844485671 39.0 35.0 41.0 31.0 41.0 64-65 36.09423533180149 38.5 35.0 40.5 31.0 41.0 66-67 35.72914491672881 37.5 35.0 40.0 30.5 41.0 68-69 35.333070313178894 37.0 35.0 40.0 30.0 41.0 70-71 34.943652352630906 36.5 35.0 39.0 30.0 41.0 72-73 34.54684552643438 36.0 35.0 39.0 29.0 40.5 74-75 34.16573826513806 35.5 34.0 38.5 29.0 40.0 76-77 33.79052672741493 35.0 34.0 37.0 29.0 39.0 78-79 33.43840872301341 35.0 34.0 37.0 29.0 39.0 80-81 33.10928270170099 35.0 34.0 36.0 29.0 38.5 82-83 32.85086908810923 35.0 34.0 36.0 29.0 37.0 84-85 32.61391955604866 35.0 34.0 35.5 29.0 37.0 86-87 32.3896360156741 35.0 34.0 35.0 28.0 36.5 88-89 32.24708030450924 35.0 34.0 35.0 28.0 36.0 90-91 32.079686821103856 35.0 34.0 35.0 27.0 36.0 92-93 31.924720105329314 35.0 33.0 35.0 27.0 36.0 94-95 31.80547183976687 35.0 33.0 35.0 27.0 35.0 96-97 31.662311727889836 35.0 33.0 35.0 26.5 35.0 98-99 31.527161504277235 35.0 33.0 35.0 26.0 35.0 100-101 30.916316545715794 34.5 32.0 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19767800479350228 1101 2 0.1967758692413355 1101 3 0.2199421260089096 1101 4 0.13793414474995558 1101 5 0.15375021604192796 1101 6 0.17252071280028503 1101 7 0.17681707048568285 1101 8 0.13011497473429756 1101 9 0.21778270705347325 1101 10-11 0.19086843217768745 1101 12-13 0.17825473789812207 1101 14-15 0.23748637509715564 1101 16-17 0.24125312651536746 1101 18-19 0.19452280962593704 1101 20-21 0.23117078369639188 1101 22-23 0.25949747706452087 1101 24-25 0.2543222792451161 1101 26-27 0.22334440586628546 1101 28-29 0.2532654161718213 1101 30-31 0.22233848983837134 1101 32-33 0.24861370692291018 1101 34-35 0.27527651354593274 1101 36-37 0.28841179023450536 1101 38-39 0.2067427740912109 1101 40-41 0.08566409197795366 1101 42-43 0.10095697371382073 1101 44-45 0.1491553453885004 1101 46-47 0.18280778652657403 1101 48-49 0.25179314597368574 1101 50-51 0.1749302298769564 1101 52-53 0.19451584472349026 1101 54-55 0.2686335504778796 1101 56-57 0.25542978649175296 1101 58-59 0.24230047398062737 1101 60-61 0.17508595085840284 1101 62-63 0.1660490447952725 1101 64-65 0.18390632531943396 1101 66-67 0.1868192798003463 1101 68-69 0.17408726343638392 1101 70-71 0.15787334859202673 1101 72-73 0.13545735893865185 1101 74-75 0.09951238979756738 1101 76-77 0.07543338624363827 1101 78-79 0.08301403241703298 1101 80-81 0.09803657812872757 1101 82-83 0.11514160122113282 1101 84-85 0.11383098039866724 1101 86-87 0.08617273673770143 1101 88-89 0.11799920187070256 1101 90-91 0.11646290901164491 1101 92-93 0.10462043590069214 1101 94-95 0.12974645466400858 1101 96-97 0.1454734211384796 1101 98-99 0.15076367354168596 1101 100-101 0.21573248117238109 1102 1 0.1500616593424482 1102 2 0.15872116508371903 1102 3 0.1851834359195763 1102 4 0.13020541477103365 1102 5 0.11567089536072217 1102 6 0.047003941956255346 1102 7 0.12914721969823262 1102 8 0.039655661466333925 1102 9 0.017695703198484125 1102 10-11 0.05569369257148793 1102 12-13 0.14798529836192387 1102 14-15 0.21694277219280167 1102 16-17 0.21400513823761003 1102 18-19 0.21976190648086913 1102 20-21 0.21389654391329316 1102 22-23 0.18915129262567376 1102 24-25 0.18494583995256164 1102 26-27 0.21588618225536038 1102 28-29 0.20040981260056867 1102 30-31 0.18623526666659274 1102 32-33 0.20355578291805188 1102 34-35 0.19966448206496779 1102 36-37 0.21150771627677045 1102 38-39 0.17575500444947778 1102 40-41 0.04926430693216588 1102 42-43 0.13588752296348616 1102 44-45 0.10426820626917532 1102 46-47 0.1429999403012019 1102 48-49 0.20857248992670208 1102 50-51 0.1407407416932358 1102 52-53 0.1511220369129873 1102 54-55 0.09517066654867534 1102 56-57 0.13059052579130537 1102 58-59 0.1032452875157972 1102 60-61 0.0641095814309196 1102 62-63 0.0011160840095314484 1102 64-65 0.012535215720077986 1102 66-67 0.03569112005543218 1102 68-69 0.054711032548176775 1102 70-71 0.04544800821066275 1102 72-73 0.04951523977402417 1102 74-75 -0.02678329727436335 1102 76-77 -0.05279597564705085 1102 78-79 -5.605909789281327E-4 1102 80-81 -0.030903293742291282 1102 82-83 0.009076607842928297 1102 84-85 -0.05349514214408657 1102 86-87 -0.044703035412837266 1102 88-89 -0.05040366160510246 1102 90-91 -0.0662864480361236 1102 92-93 -0.05675332093013097 1102 94-95 -0.07720138420766887 1102 96-97 -0.029542836881990553 1102 98-99 -0.027609479908921486 1102 100-101 -0.034560369119358114 1103 1 0.16925257543634586 1103 2 0.15676496831750342 1103 3 0.18845631736660096 1103 4 0.14171945883701653 1103 5 0.14585653062306392 1103 6 0.125816879495936 1103 7 0.1219897720935208 1103 8 0.1350147400975743 1103 9 0.1422397064788541 1103 10-11 0.14642016022663995 1103 12-13 0.1363177956797088 1103 14-15 0.20049320084981304 1103 16-17 0.2013454244336046 1103 18-19 0.1813066741183107 1103 20-21 0.20973910825165376 1103 22-23 0.16954877820938208 1103 24-25 0.2281725016827565 1103 26-27 0.22704296900968757 1103 28-29 0.24716743576855293 1103 30-31 0.26851207539618116 1103 32-33 0.25918290405869726 1103 34-35 0.25296283212855997 1103 36-37 0.2642789187258927 1103 38-39 0.2448917586788184 1103 40-41 0.20787956660456786 1103 42-43 0.16803885969264343 1103 44-45 0.19267318812564582 1103 46-47 0.25002499887205687 1103 48-49 0.32407157130486297 1103 50-51 0.2679652795458267 1103 52-53 0.2767439329564425 1103 54-55 0.22610106228261628 1103 56-57 0.1899249081251 1103 58-59 0.20689200724954304 1103 60-61 0.16783655865746283 1103 62-63 0.13053953185708878 1103 64-65 0.16468890125481295 1103 66-67 0.12950991965653458 1103 68-69 0.11792628863765486 1103 70-71 0.12083297346624988 1103 72-73 0.12398396184464389 1103 74-75 0.13549914460762125 1103 76-77 0.1273186978192129 1103 78-79 0.14338989102384403 1103 80-81 0.1167220239933755 1103 82-83 0.03606037321715405 1103 84-85 0.09219208254305755 1103 86-87 0.10867361148804378 1103 88-89 0.08590455344198489 1103 90-91 0.1014209684170595 1103 92-93 0.14067912602767052 1103 94-95 0.13996752875316076 1103 96-97 0.10708651761447996 1103 98-99 0.13958170689477 1103 100-101 0.20463330383533673 1104 1 0.16745897213361616 1104 2 0.1634369028230367 1104 3 0.19750974965202772 1104 4 0.1326611612519102 1104 5 0.13317502124712632 1104 6 0.12156216754912919 1104 7 0.14413664082108113 1104 8 0.13786818178498805 1104 9 0.13340924659773634 1104 10-11 0.13051646716606768 1104 12-13 0.1462265171480155 1104 14-15 0.1916739941264467 1104 16-17 0.2187602751464368 1104 18-19 0.20273407620236128 1104 20-21 0.18809527166346385 1104 22-23 0.21647302922232825 1104 24-25 0.21603696846391784 1104 26-27 0.20362915706047602 1104 28-29 0.22645161942803327 1104 30-31 0.1992692115609529 1104 32-33 0.2599152767827988 1104 34-35 0.25570782289177885 1104 36-37 0.27662237340429385 1104 38-39 0.2445721567002792 1104 40-41 0.16694287116844464 1104 42-43 0.1700228172303042 1104 44-45 0.21334067369095777 1104 46-47 0.2310776427132808 1104 48-49 0.27630969918161696 1104 50-51 0.25563720295316017 1104 52-53 0.2620903833213646 1104 54-55 0.2125551578899163 1104 56-57 0.22112002407656206 1104 58-59 0.23312827842168105 1104 60-61 0.22917259474265705 1104 62-63 0.2321197969343558 1104 64-65 0.2022052467431621 1104 66-67 0.20936748995165289 1104 68-69 0.23991734675798426 1104 70-71 0.21666185169697627 1104 72-73 0.1795206000699494 1104 74-75 0.18806061518473882 1104 76-77 0.17919509583035875 1104 78-79 0.15513873868572858 1104 80-81 0.20022003632413998 1104 82-83 0.22908819364023714 1104 84-85 0.21735241113939452 1104 86-87 0.2537322827714519 1104 88-89 0.2450088996865034 1104 90-91 0.2694071086196743 1104 92-93 0.2201662940235991 1104 94-95 0.27896440805451306 1104 96-97 0.28474610096890984 1104 98-99 0.32419046320630684 1104 100-101 0.2922068925998218 1105 1 0.14428455278179797 1105 2 0.11814342481513052 1105 3 0.1429399913912306 1105 4 0.1032717912350094 1105 5 0.13170829597370215 1105 6 0.11661320475256076 1105 7 0.1199586027509909 1105 8 0.09366875020025134 1105 9 0.11072225082282472 1105 10-11 0.10922172822495924 1105 12-13 0.09823160049847246 1105 14-15 0.15642396083935495 1105 16-17 0.18367784585839786 1105 18-19 0.1491028798444063 1105 20-21 0.14744826958290247 1105 22-23 0.17376595970691966 1105 24-25 0.16367250158509705 1105 26-27 0.15567748801943537 1105 28-29 0.16549524472126365 1105 30-31 0.19901062608855113 1105 32-33 0.21920762851836884 1105 34-35 0.21360946200248776 1105 36-37 0.18959652422476836 1105 38-39 0.20302169480616072 1105 40-41 0.18335024246569986 1105 42-43 0.16562263897053953 1105 44-45 0.17193288889698266 1105 46-47 0.2169381093590772 1105 48-49 0.2831064628904798 1105 50-51 0.22063769012302714 1105 52-53 0.22800968701144342 1105 54-55 0.24495841881033442 1105 56-57 0.18176991843851908 1105 58-59 0.19868384893955948 1105 60-61 0.16790383364075723 1105 62-63 0.08641376386857758 1105 64-65 0.18739899812727856 1105 66-67 0.1601419558688164 1105 68-69 0.17498391192157925 1105 70-71 0.176683294489699 1105 72-73 0.14136898353689276 1105 74-75 0.1286397916076112 1105 76-77 0.07312904545187138 1105 78-79 0.13274720155575181 1105 80-81 0.11776266222389609 1105 82-83 0.13507906568210615 1105 84-85 0.10596502161315158 1105 86-87 0.1701000413280127 1105 88-89 0.19059250357354074 1105 90-91 0.18606945003757858 1105 92-93 0.16181284084306924 1105 94-95 0.20259784290555416 1105 96-97 0.2151501553151114 1105 98-99 0.198234921103829 1105 100-101 0.23529267874928195 1106 1 0.05880541562977015 1106 2 0.08746592928120123 1106 3 0.13971955496151622 1106 4 0.06482796210889319 1106 5 0.09528058215264679 1106 6 0.054380177373055005 1106 7 0.09214569426497832 1106 8 0.08820163476834608 1106 9 0.09461518086843057 1106 10-11 0.08612425434571236 1106 12-13 0.10186034463856686 1106 14-15 0.06775248263183897 1106 16-17 0.11254817503214554 1106 18-19 0.14321386308645856 1106 20-21 0.16144523857272475 1106 22-23 0.1369687600963445 1106 24-25 0.12905067036199114 1106 26-27 0.11639263772607933 1106 28-29 0.13649785161444328 1106 30-31 0.1515705906224767 1106 32-33 0.1752565925412668 1106 34-35 0.1718787831471147 1106 36-37 0.16594729884689485 1106 38-39 0.1313550405016457 1106 40-41 0.14905851881426457 1106 42-43 0.12251673219982706 1106 44-45 0.1615242968570314 1106 46-47 0.20787787656639267 1106 48-49 0.21455710185222188 1106 50-51 0.1873621783916306 1106 52-53 0.14964528377925745 1106 54-55 0.11354043971074645 1106 56-57 0.17149526083692734 1106 58-59 0.1262710434968639 1106 60-61 0.1923774102955278 1106 62-63 0.1924031755878559 1106 64-65 0.18478396019353482 1106 66-67 0.18920254336025266 1106 68-69 0.18936004721908972 1106 70-71 0.19157075228482512 1106 72-73 0.1795550522252114 1106 74-75 0.08259878595963244 1106 76-77 0.05683320063837982 1106 78-79 0.13971181612372874 1106 80-81 0.14434970139841852 1106 82-83 0.1521336187302964 1106 84-85 0.1044136187272926 1106 86-87 0.12025577595096593 1106 88-89 0.1125867787547179 1106 90-91 0.1038946075171765 1106 92-93 0.12219471160986473 1106 94-95 0.12004340615671083 1106 96-97 0.09730439107433853 1106 98-99 0.12303273330671871 1106 100-101 0.10776968656767849 1107 1 0.046870227160525246 1107 2 0.09178523883800693 1107 3 0.09268290700661908 1107 4 0.07600245323968835 1107 5 0.07328400172845306 1107 6 0.009304379762760107 1107 7 0.04078395275062263 1107 8 0.04195287814529536 1107 9 0.061886961935080365 1107 10-11 0.05204899628187576 1107 12-13 0.05416341146733572 1107 14-15 0.06861128767231861 1107 16-17 0.10370381986250976 1107 18-19 0.0684504691908856 1107 20-21 0.06366572820562055 1107 22-23 0.02894834253027767 1107 24-25 0.026651541348321928 1107 26-27 0.11361330510230516 1107 28-29 0.06629081957459704 1107 30-31 0.11956243411375311 1107 32-33 0.053536608632178684 1107 34-35 0.14378176922753028 1107 36-37 0.06900862638824634 1107 38-39 0.0740740333081078 1107 40-41 0.028884947688759155 1107 42-43 0.071274315578151 1107 44-45 0.050239452464332146 1107 46-47 0.10841130116087783 1107 48-49 0.1110219763569873 1107 50-51 0.07152831690846995 1107 52-53 0.09088992513033389 1107 54-55 0.0738954717085818 1107 56-57 0.010223174301486893 1107 58-59 0.06916580571706987 1107 60-61 0.04747410633367366 1107 62-63 0.10467558242851283 1107 64-65 0.012352907524878276 1107 66-67 -0.072608353318806 1107 68-69 -0.005371163384054967 1107 70-71 0.015364058765207744 1107 72-73 0.019907912375956016 1107 74-75 -0.022120086172336073 1107 76-77 0.005675303902982876 1107 78-79 -0.037288258939767616 1107 80-81 -0.01439425266624994 1107 82-83 -0.0074154317788384105 1107 84-85 -0.041699487426086534 1107 86-87 0.006441780001310349 1107 88-89 -0.005017097709554719 1107 90-91 -0.06541558094289712 1107 92-93 0.00647462054064718 1107 94-95 -0.026658052418188305 1107 96-97 -0.01893740785257947 1107 98-99 -0.0114608715694402 1107 100-101 0.03387180899184017 1108 1 -0.007453341197852126 1108 2 0.02296021824392369 1108 3 0.05325864621151055 1108 4 0.01653823055018222 1108 5 0.06147842207732879 1108 6 0.04580856240703923 1108 7 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94-95 3.356693611777928E-5 2213 96-97 0.010869870925660052 2213 98-99 0.016774236070848048 2213 100-101 -0.060968061506926574 2214 1 -0.08565710647981462 2214 2 -0.09530251298836845 2214 3 -0.11187742741307716 2214 4 -0.065429624592511 2214 5 -0.10174146605699974 2214 6 -0.09023391388119961 2214 7 -0.0986747190571009 2214 8 -0.10106994658671198 2214 9 -0.17824795712078156 2214 10-11 -0.16338003645066834 2214 12-13 -0.14073016686513284 2214 14-15 -0.2070308868128663 2214 16-17 -0.1991669849965092 2214 18-19 -0.1704401975848029 2214 20-21 -0.17952152340201621 2214 22-23 -0.21431438335228847 2214 24-25 -0.22073916746357725 2214 26-27 -0.2178839124135692 2214 28-29 -0.22770525803446873 2214 30-31 -0.3254172946743594 2214 32-33 -0.23754856460860196 2214 34-35 -0.29987247809705053 2214 36-37 -0.3326108857910839 2214 38-39 -0.2566921739114747 2214 40-41 -0.20020046865159458 2214 42-43 -0.19198762322302798 2214 44-45 -0.1384449199734874 2214 46-47 -0.15400288203928625 2214 48-49 -0.21457829039368193 2214 50-51 -0.18402859286953088 2214 52-53 -0.14747777994734435 2214 54-55 -0.1462454456613571 2214 56-57 -0.19757479347241258 2214 58-59 -0.15989131733486062 2214 60-61 -0.12085671755351513 2214 62-63 -0.1373685244784042 2214 64-65 -0.20885689448001443 2214 66-67 -0.18219156980212148 2214 68-69 -0.16012201829604322 2214 70-71 -0.11947685478531156 2214 72-73 -0.11552244032075976 2214 74-75 -0.09846834805226479 2214 76-77 -0.09555564563854091 2214 78-79 -0.15668725705894104 2214 80-81 -0.12639940967633834 2214 82-83 -0.14550574825660334 2214 84-85 -0.15061493921104585 2214 86-87 -0.09762776704816645 2214 88-89 -0.13369209000909166 2214 90-91 -0.18902582089752684 2214 92-93 -0.21580169607567967 2214 94-95 -0.20383992949711427 2214 96-97 -0.16601431185778992 2214 98-99 -0.18428050188185452 2214 100-101 -0.2339135200527238 2215 1 -0.12483668526404301 2215 2 -0.12838058715962575 2215 3 -0.11708393364708769 2215 4 -0.06568735604531639 2215 5 -0.1499551690641141 2215 6 -0.13401951458155992 2215 7 -0.12437381354178001 2215 8 -0.14463052651591823 2215 9 -0.19312769276714192 2215 10-11 -0.1770964445038672 2215 12-13 -0.21073470383771564 2215 14-15 -0.23569993152631952 2215 16-17 -0.23863317851223087 2215 18-19 -0.23550283418727957 2215 20-21 -0.2669460660748797 2215 22-23 -0.285232368971144 2215 24-25 -0.2761015417777841 2215 26-27 -0.30331513079860883 2215 28-29 -0.2795111686031788 2215 30-31 -0.27900309883649044 2215 32-33 -0.30366561170317397 2215 34-35 -0.3190171372314552 2215 36-37 -0.369905766041164 2215 38-39 -0.2870027166574687 2215 40-41 -0.22696679811625842 2215 42-43 -0.20653913175289063 2215 44-45 -0.20756221048880263 2215 46-47 -0.17668701312305757 2215 48-49 -0.16511620022850337 2215 50-51 -0.2661960129015952 2215 52-53 -0.29463271057569074 2215 54-55 -0.2360369483107121 2215 56-57 -0.22178357147383565 2215 58-59 -0.20345325341680365 2215 60-61 -0.2706689566454301 2215 62-63 -0.186726241174469 2215 64-65 -0.17500096959469857 2215 66-67 -0.1905033831418379 2215 68-69 -0.14678349995028128 2215 70-71 -0.17258272273475228 2215 72-73 -0.18897810508279633 2215 74-75 -0.1607823665310235 2215 76-77 -0.1045405859126376 2215 78-79 -0.10080187428850707 2215 80-81 -0.07832634942027994 2215 82-83 -0.0933591901545725 2215 84-85 -0.07127724806989733 2215 86-87 -0.07173143211304023 2215 88-89 -0.10971658588740496 2215 90-91 -0.11204841822442901 2215 92-93 -0.060243559205350294 2215 94-95 -0.058639834085006726 2215 96-97 -0.02806897115361906 2215 98-99 -0.06993310856038448 2215 100-101 -0.13320820998358585 2216 1 -0.2222294098231643 2216 2 -0.2331344728104554 2216 3 -0.1942412546670269 2216 4 -0.1527928850203253 2216 5 -0.1751926187236279 2216 6 -0.26780535501391256 2216 7 -0.2518240288727327 2216 8 -0.2059163540122384 2216 9 -0.33883865611070974 2216 10-11 -0.31998914655461164 2216 12-13 -0.36187612819853854 2216 14-15 -0.43730777279837696 2216 16-17 -0.49499786433317183 2216 18-19 -0.4930448419315496 2216 20-21 -0.47400868905507565 2216 22-23 -0.48970333075997274 2216 24-25 -0.5167980381847173 2216 26-27 -0.4830617068765264 2216 28-29 -0.5171121128359815 2216 30-31 -0.5276672270117473 2216 32-33 -0.5605409827070744 2216 34-35 -0.5938209339361862 2216 36-37 -0.5938627899594806 2216 38-39 -0.5156082925745125 2216 40-41 -0.43265190893934147 2216 42-43 -0.3819391938318333 2216 44-45 -0.37739704453208134 2216 46-47 -0.41949438161042707 2216 48-49 -0.4771269768419941 2216 50-51 -0.5505379485961512 2216 52-53 -0.463915758502651 2216 54-55 -0.4374171902406303 2216 56-57 -0.4071654035839245 2216 58-59 -0.3874423842749053 2216 60-61 -0.34991551332089443 2216 62-63 -0.3283287914352684 2216 64-65 -0.26004377143299706 2216 66-67 -0.313151246148216 2216 68-69 -0.3152894823119752 2216 70-71 -0.23840638699833505 2216 72-73 -0.2115863854288449 2216 74-75 -0.18991067484589053 2216 76-77 -0.17239553146314535 2216 78-79 -0.1559273352256625 2216 80-81 -0.2012484021613119 2216 82-83 -0.17163132486484756 2216 84-85 -0.14011829142661014 2216 86-87 -0.0859349494873527 2216 88-89 -0.1229638856160733 2216 90-91 -0.1316328085014149 2216 92-93 -0.14155077611682287 2216 94-95 -0.19561267789910985 2216 96-97 -0.1312609720117166 2216 98-99 -0.1789045345601572 2216 100-101 -0.2181162097982252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 21.0 13 180.0 14 711.0 15 1825.0 16 3816.0 17 7747.0 18 19051.0 19 55198.0 20 73713.0 21 69831.0 22 66962.0 23 63813.0 24 62498.0 25 63039.0 26 67069.0 27 79610.0 28 95243.0 29 111712.0 30 133443.0 31 163612.0 32 209220.0 33 278121.0 34 396863.0 35 607617.0 36 883549.0 37 2057760.0 38 3496039.0 39 828009.0 40 308.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.545598992487697 30.176957509241685 23.026849615729017 23.2505938825416 2 20.565151531839273 26.587704605041324 33.59235581388981 19.254788049229596 3 19.84374400045268 28.612227658443622 23.722063581560498 27.821964759543192 4 26.60995010397531 23.68650584343278 27.861483461963626 21.84206059062828 5 21.690261900068116 30.10912578622008 24.01498864100368 24.185623672708125 6 22.297379498776344 23.92206196484038 26.7308403509091 27.049718185474177 7 28.032461719098922 24.493522004571275 23.304070699170826 24.169945577158977 8 31.652671933132453 24.032231336481896 23.56084627214654 20.75425045823911 9 21.297195596862757 27.451220522645198 23.92967065390266 27.321913226589388 10-11 28.092608759793787 23.802636870514863 23.5431583435894 24.561596026101945 12-13 26.210771801976033 23.970644404430622 23.596383801272765 26.222199992320576 14-15 25.70614293018396 24.311428796614585 23.928741039833962 26.053687233367484 16-17 25.639286500993276 27.095723977374004 24.10662572322964 23.158363798403084 18-19 22.291650246852953 24.44520228200045 27.522376416903615 25.740771054242984 20-21 21.951749998484324 29.008854574004356 25.104909979002848 23.934485448508475 22-23 19.967796956120196 26.78715778582096 30.642449209726998 22.602596048331847 24-25 19.973218020770812 23.830909263604195 33.948045688510575 22.24782702711442 26-27 19.98329726026567 23.8242706066136 36.04030382212845 20.152128310992282 28-29 19.987283485810252 23.826599694035718 36.06393824937504 20.122178570778996 30-31 20.005047198122988 23.78357472985617 36.04652819458843 20.164849877432406 32-33 20.00850798962874 23.836557679521615 36.06184661772047 20.093087713129183 34-35 20.025726058901157 23.869978315741395 36.0161894310964 20.088106194261048 36-37 20.06184964907069 23.883361726980432 35.85334529706222 20.20144332688666 38-39 20.28550266859865 24.049636339018125 35.16234396124722 20.50251703113601 40-41 21.194821847547335 24.8991772915492 32.32156462131363 21.584436239589838 42-43 21.129738758237696 25.218186484623985 32.51037732226688 21.14169743487144 44-45 21.2958971685168 25.724053157757528 31.878871286848586 21.101178386877084 46-47 21.237861968478 26.103330645536133 31.535944740506316 21.122862645479547 48-49 21.327261538834627 26.337249837822764 30.98118744050975 21.354301182832856 50-51 21.37211541764933 26.485846625804065 30.750547158715435 21.39149079783117 52-53 21.42701822245665 26.49816401221432 30.589577409569767 21.485240355759263 54-55 21.512719545539973 26.419510578401834 30.39815774742386 21.669612128634334 56-57 21.58072788781579 26.47142750323849 30.203585481044964 21.74425912790075 58-59 21.670718571466104 26.577873366354844 29.995225623397175 21.75618243878188 60-61 21.730021886348617 26.629689246184036 29.70918741625895 21.9311014512084 62-63 21.832900860701372 26.76257858775456 29.44541447651613 21.95910607502794 64-65 21.903010939132507 26.917323964440243 29.162149954832884 22.01751514159437 66-67 21.979208979263543 26.96105119142168 28.90448013354108 22.15525969577369 68-69 22.057058879966096 27.07010052425758 28.708345465066092 22.164495130710232 70-71 22.111881074068013 27.169810176848973 28.49935533285236 22.218953416230658 72-73 22.165384405521905 27.187882076434484 28.31207346376223 22.33466005428138 74-75 22.158710382778697 27.263478898771094 28.243938815227075 22.333871903223134 76-77 22.20105834540821 27.2748565666119 28.16016745178637 22.363917636193513 78-79 22.256900868784975 27.217306382608943 28.041065701484758 22.48472704712133 80-81 22.224884758168983 27.288325866107282 28.027000236445314 22.459789139278417 82-83 22.234221316858953 27.32044807398111 27.993301726455 22.452028882704937 84-85 22.2554357161767 27.299410503426436 27.914102649602185 22.53105113079468 86-87 22.27389663904096 27.331704487812964 27.847018869144698 22.547380004001383 88-89 22.28187919463087 27.34468371902213 27.884895590193786 22.488541496153218 90-91 22.316724565455946 27.30090091728658 27.81060224845351 22.571772268803972 92-93 22.31223311487403 27.27875690390014 27.850525130903808 22.55848485032203 94-95 22.345431452077385 27.34004070092901 27.835338066281484 22.479189780712126 96-97 22.36400352444986 27.30416467102777 27.823914928187314 22.507916876335056 98-99 22.36298296987444 27.338075375533773 27.790135582191017 22.50880607240077 100-101 22.379327637840284 27.384999923709348 27.67393435345894 22.561738084991426 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11239.0 1 6251.0 2 932.0 3 482.5 4 330.0 5 274.5 6 243.5 7 225.5 8 207.5 9 209.0 10 277.5 11 315.0 12 344.0 13 744.5 14 1417.5 15 4571.0 16 31847.0 17 34918.0 18 13584.5 19 13296.0 20 13204.5 21 14865.0 22 18120.5 23 22304.0 24 27736.0 25 35190.5 26 44789.5 27 56650.5 28 71035.5 29 87652.0 30 106708.5 31 127639.0 32 148836.0 33 170240.5 34 191197.0 35 215115.0 36 244182.0 37 274221.0 38 305967.5 39 336740.5 40 362010.0 41 382098.0 42 401353.0 43 418704.0 44 428577.0 45 432001.0 46 431595.0 47 426569.5 48 415007.0 49 398022.5 50 378004.5 51 358429.0 52 339805.0 53 318037.0 54 291408.5 55 260406.5 56 226995.5 57 195007.5 58 166182.5 59 138657.5 60 111656.5 61 88439.5 62 69672.0 63 54223.5 64 42001.0 65 32093.0 66 24060.0 67 18104.5 68 13805.5 69 10656.5 70 8529.5 71 7054.0 72 5753.5 73 4561.0 74 3318.0 75 2197.5 76 1380.0 77 848.0 78 481.5 79 234.0 80 93.5 81 31.5 82 17.0 83 7.0 84 1.5 85 1.0 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18981304652718411 2 1.3135850970739387E-4 3 0.0 4 0.0 5 1.9198551418772952E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.3641076008075517E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0021926766620388055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9896580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.89669123739576 #Duplication Level Percentage of deduplicated Percentage of total 1 82.27710813575627 53.395120825919975 2 10.189973254167752 13.22591095986091 3 3.240436699474023 6.3088085988027425 4 1.4543721654338249 3.7753576545768635 5 0.7802518605940939 2.5317882042189233 6 0.4811549357380563 1.873521798116498 7 0.30145980164570274 1.3694620567521418 8 0.235251203917847 1.2213619795105715 9 0.162400728354606 0.9485342932281343 >10 0.8521601777869768 9.338253815037483 >50 0.018763696444886502 0.8134564878102881 >100 0.005356929822463442 0.6302582139699744 >500 5.464225049820826E-4 0.23917164790341616 >1k 5.924810227468753E-4 0.8371884956295005 >5k 9.354945585618842E-5 0.41435929129948174 >10k+ 7.795787988015703E-5 3.0774456773631482 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 224277 2.266207113972706 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31381 0.31708933793290206 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22931 0.23170630662309608 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13912 0.14057381438840488 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 11642 0.11763659769334457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0209001493445212E-5 1.0104500746722606E-5 0.0 0.0 0.0 2 4.0418002986890423E-5 1.0104500746722606E-5 0.0 0.0 0.0 3 5.0522503733613024E-5 1.0104500746722606E-5 0.0 0.0 0.0 4 6.062700448033563E-5 1.0104500746722606E-5 0.0 0.0 0.0 5 8.083600597378085E-5 1.0104500746722606E-5 0.0 0.0 0.0 6 1.1114950821394866E-4 1.0104500746722606E-5 0.0 0.0 0.0 7 1.3135850970739387E-4 1.0104500746722606E-5 0.0 0.0 0.0 8 1.3135850970739387E-4 1.0104500746722606E-5 0.0 0.0 0.0 9 1.616720119475617E-4 1.0104500746722606E-5 0.0 0.0 0.0 10-11 1.9703776456109082E-4 1.0104500746722606E-5 0.0 0.0 0.0 12-13 3.33448524641846E-4 1.0104500746722606E-5 0.0 0.0 0.0 14-15 4.749115350959624E-4 1.0104500746722606E-5 0.0 0.0 0.0 16-17 5.911132936832725E-4 1.0104500746722606E-5 0.0 0.0 0.0 18-19 6.871060507771371E-4 1.0104500746722606E-5 0.0 0.0 0.0 20-21 8.083600597378084E-4 1.5156751120083908E-5 0.0 0.0 0.0 22-23 9.296140686984797E-4 2.0209001493445212E-5 0.0 0.0 0.0 24-25 0.0010761293295259574 2.0209001493445212E-5 0.0 0.0 0.0 26-27 0.0013236895978206613 2.0209001493445212E-5 0.0 5.052250373361303E-6 0.0 28-29 0.0016167201194756168 2.0209001493445212E-5 0.0 1.0104500746722606E-5 0.0 30-31 0.0018743848885170431 2.5261251866806512E-5 0.0 1.0104500746722606E-5 1.0104500746722606E-5 32-33 0.0020512136515846886 3.0313502240167816E-5 0.0 1.5156751120083908E-5 2.0209001493445212E-5 34-35 0.0028039989572155232 4.0418002986890423E-5 0.0 2.0209001493445212E-5 2.0209001493445212E-5 36-37 0.006375939971181964 4.0418002986890423E-5 0.0 2.0209001493445212E-5 2.0209001493445212E-5 38-39 0.0065931867372365 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 40-41 0.006694231744703726 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 42-43 0.006815485753664398 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 44-45 0.007002419017478765 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 46-47 0.007153986528679605 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 48-49 0.007300501789507082 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 50-51 0.007431860299214476 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 52-53 0.007613741312655483 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 54-55 0.0077552043231096 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 56-57 0.007891615083190355 4.0418002986890423E-5 0.0 3.0313502240167816E-5 2.0209001493445212E-5 58-59 0.008093705098124807 4.0418002986890423E-5 0.0 3.536575261352912E-5 2.0209001493445212E-5 60-61 0.008270533861192452 4.0418002986890423E-5 0.0 4.0418002986890423E-5 2.0209001493445212E-5 62-63 0.008502937378367072 4.0418002986890423E-5 0.0 4.0418002986890423E-5 2.0209001493445212E-5 64-65 0.008826281402262196 5.0522503733613024E-5 0.0 6.062700448033563E-5 2.0209001493445212E-5 66-67 0.009195095679517571 5.0522503733613024E-5 0.0 6.062700448033563E-5 2.5261251866806512E-5 68-69 0.009513387453039333 5.0522503733613024E-5 0.0 6.062700448033563E-5 3.536575261352912E-5 70-71 0.010074187244482436 5.0522503733613024E-5 0.0 6.062700448033563E-5 4.0418002986890423E-5 72-73 0.01093306980795386 6.062700448033563E-5 0.0 6.062700448033563E-5 4.0418002986890423E-5 74-75 0.011928363131506036 6.062700448033563E-5 0.0 6.062700448033563E-5 4.0418002986890423E-5 76-77 0.013055014964765606 6.062700448033563E-5 0.0 6.062700448033563E-5 4.0418002986890423E-5 78-79 0.014499958571546939 6.062700448033563E-5 0.0 6.062700448033563E-5 4.0418002986890423E-5 80-81 0.016566328974251712 6.062700448033563E-5 0.0 6.062700448033563E-5 4.547025336025172E-5 82-83 0.019365275681093874 6.062700448033563E-5 0.0 6.062700448033563E-5 6.567925485369694E-5 84-85 0.0235788524924772 7.073150522705824E-5 0.0 6.567925485369694E-5 7.073150522705824E-5 86-87 0.02936873142034925 7.073150522705824E-5 0.0 7.073150522705824E-5 8.083600597378085E-5 88-89 0.03720477174943263 7.578375560041954E-5 0.0 8.588825634714214E-5 8.083600597378085E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 56935 0.0 93.64836 1 TCGACGG 58255 0.0 91.491875 2 TATCTCG 41470 0.0 89.30538 2 CGACGGC 61465 0.0 86.62099 3 ATATCTC 43060 0.0 86.454765 1 ACGGCGC 64340 0.0 83.95372 5 GACGGCG 63935 0.0 83.906044 4 CGGCGCG 64845 0.0 83.1827 6 ATCTCGA 47895 0.0 77.28561 3 TCTCGAG 48350 0.0 73.46376 4 TCGAGGG 52955 0.0 66.79725 6 CTCGAGG 52930 0.0 66.78392 5 CGAGGGC 54450 0.0 64.963234 7 AGGGCGC 56790 0.0 63.457417 9 GAGGGCG 57155 0.0 62.79454 8 TACGGCG 765 0.0 50.293026 4 CGCGCCG 122110 0.0 47.69346 9 GGCGCGC 123130 0.0 47.637844 7 GCGCGCC 123470 0.0 47.52205 8 ATCCATA 71075 0.0 38.410175 16-17 >>END_MODULE