FastQCFastQC Report
Tue 31 May 2016
SRR1567938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10361752
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980811.911655480656167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG29650.076.572621
TCGACGG36850.062.2558142
CGACGGC62500.036.5540353
ATCTCGA33750.031.38351
ACGGCGC77250.029.6974355
GACGGCG78300.029.4811744
CGGCGCG85000.026.822096
GGGCGCG38400.026.3462438
TCTCGAG40850.024.9986572
CGCGCCG118450.024.3007939
GGCGCGC121500.023.924647
GCGCGCC124000.023.5188988
TCGAGGG44400.022.6789634
CTCGAGG46050.022.0726493
AGGGCGC46800.021.92197
CGAGGGC52700.019.3775185
GAGGGCG61850.016.8180126
CGCCGGA119150.016.4242699
TATCTCG17250.015.6948092
GCGCCGG128650.015.2848388
Produced by FastQC (version 0.11.5)