##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567938_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10361752 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.842958217876667 33.0 31.0 34.0 30.0 34.0 2 32.05064404166399 34.0 31.0 34.0 30.0 34.0 3 32.007855042274706 34.0 31.0 34.0 30.0 34.0 4 35.34905187848541 37.0 35.0 37.0 32.0 37.0 5 35.22203996003765 37.0 35.0 37.0 32.0 37.0 6 35.27056978395159 37.0 35.0 37.0 32.0 37.0 7 35.24445132444784 37.0 35.0 37.0 32.0 37.0 8 35.20772963877151 37.0 35.0 37.0 32.0 37.0 9 36.77298781132766 39.0 37.0 39.0 32.0 39.0 10-11 36.742400126928345 39.0 37.0 39.0 32.0 39.0 12-13 36.68367801120891 39.0 37.0 39.0 32.0 39.0 14-15 37.94606182429381 40.0 38.0 41.0 32.0 41.0 16-17 37.963749807947536 40.0 38.0 41.0 32.0 41.0 18-19 37.98275127603903 40.0 38.0 41.0 32.0 41.0 20-21 37.977051950287944 40.0 38.0 41.0 32.0 41.0 22-23 38.006056552984475 40.0 38.0 41.0 32.0 41.0 24-25 38.01707495991025 40.0 38.0 41.0 32.5 41.0 26-27 38.01828730315105 40.0 38.0 41.0 32.5 41.0 28-29 37.93859272061327 40.0 38.0 41.0 32.0 41.0 30-31 37.931064264035655 40.0 38.0 41.0 32.5 41.0 32-33 37.93097571723392 40.0 38.0 41.0 33.0 41.0 34-35 37.859836106866865 40.0 37.0 41.0 32.0 41.0 36-37 37.82482682465282 40.0 37.0 41.0 32.0 41.0 38-39 37.812730076921355 40.0 37.0 41.0 32.0 41.0 40-41 37.74305175418211 40.0 37.0 41.0 32.0 41.0 42-43 37.63690855561878 40.0 37.0 41.0 31.0 41.0 44-45 37.57089211361168 40.0 36.5 41.0 31.0 41.0 46-47 37.515345233122744 40.0 36.0 41.0 31.0 41.0 48-49 37.43807919741758 40.0 36.0 41.0 31.0 41.0 50-51 37.23397336666618 39.5 36.0 41.0 30.0 41.0 52-53 37.17128445073767 39.0 35.0 41.0 30.0 41.0 54-55 37.321930789310535 39.0 36.0 41.0 31.0 41.0 56-57 37.37548182006286 39.0 35.5 41.0 31.0 41.0 58-59 37.24006056118695 39.0 35.0 41.0 31.0 41.0 60-61 37.053027470643954 39.0 35.0 41.0 31.0 41.0 62-63 36.7975718295516 39.0 35.0 40.5 31.0 41.0 64-65 36.49922098116225 38.0 35.0 40.0 31.0 41.0 66-67 36.21300147890048 37.0 35.0 40.0 30.5 41.0 68-69 35.85493891380531 37.0 35.0 39.5 30.0 41.0 70-71 35.506021568553265 36.5 34.5 39.0 30.0 41.0 72-73 35.12069701147065 36.0 34.0 39.0 30.0 40.5 74-75 34.753231355083585 35.5 34.0 37.5 30.0 40.0 76-77 34.40151308388775 35.0 34.0 37.0 29.0 39.0 78-79 34.07600799555905 35.0 34.0 37.0 29.0 39.0 80-81 33.77558548978976 35.0 34.0 36.0 29.0 38.0 82-83 33.50088686739463 35.0 34.0 36.0 29.0 37.0 84-85 33.26123810915374 35.0 33.5 35.5 29.0 37.0 86-87 33.09894861409538 35.0 33.0 35.0 29.0 36.5 88-89 32.95003504233647 35.0 33.0 35.0 29.0 36.0 90-91 32.802217858524315 35.0 33.0 35.0 29.0 36.0 92-93 32.65329072728241 35.0 33.0 35.0 28.5 36.0 94-95 32.55700623794122 35.0 33.0 35.0 28.0 35.5 96-97 32.445652434067135 35.0 33.0 35.0 28.0 35.0 98-99 32.31985358267598 35.0 33.0 35.0 28.0 35.0 100-101 31.918153416526472 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3119805588494522 1101 2 0.35747116859029404 1101 3 0.3799144470071596 1101 4 0.33173563555369867 1101 5 0.34731720815050693 1101 6 0.3568330566684139 1101 7 0.3494541988762876 1101 8 0.36570416461029254 1101 9 0.45438234334696403 1101 10-11 0.42394583180978884 1101 12-13 0.4052025592000277 1101 14-15 0.4890898098428167 1101 16-17 0.4339705343886564 1101 18-19 0.4595400365065103 1101 20-21 0.46695207982114084 1101 22-23 0.44723827881006173 1101 24-25 0.45360699384739434 1101 26-27 0.47049395303598374 1101 28-29 0.4653330346433435 1101 30-31 0.44563705919070884 1101 32-33 0.4042832449074396 1101 34-35 0.4525375136886467 1101 36-37 0.42804849916096543 1101 38-39 0.41347740477824857 1101 40-41 0.38400489493987067 1101 42-43 0.44568568364032757 1101 44-45 0.4209331390522948 1101 46-47 0.3872040490294637 1101 48-49 0.3790114087590837 1101 50-51 0.47413503000537105 1101 52-53 0.4298234270896586 1101 54-55 0.43911689175660484 1101 56-57 0.4541608954571146 1101 58-59 0.4339996792140539 1101 60-61 0.41925136769550164 1101 62-63 0.38947587115047355 1101 64-65 0.4071533866949082 1101 66-67 0.38852132503585324 1101 68-69 0.3848348395344914 1101 70-71 0.38771525184167643 1101 72-73 0.40717926261596205 1101 74-75 0.38071020641692854 1101 76-77 0.3805914096728955 1101 78-79 0.3626065376154841 1101 80-81 0.347860344159308 1101 82-83 0.34876811201522884 1101 84-85 0.3463869082768767 1101 86-87 0.3472782600098583 1101 88-89 0.34874131907996997 1101 90-91 0.3216848509607644 1101 92-93 0.3253484391748529 1101 94-95 0.3115620464815976 1101 96-97 0.32836860448227156 1101 98-99 0.33998947847009475 1101 100-101 0.3611869301798656 1102 1 0.22683430983483888 1102 2 0.3377249808303944 1102 3 0.3700573066484907 1102 4 0.3330466272468797 1102 5 0.26694793504263714 1102 6 0.33772251128517894 1102 7 0.2943768315517161 1102 8 0.30784668463818576 1102 9 0.3968551411155872 1102 10-11 0.3763958176101241 1102 12-13 0.3762221594464208 1102 14-15 0.5107430411490697 1102 16-17 0.46709649448236235 1102 18-19 0.43666746006153545 1102 20-21 0.46301385481071833 1102 22-23 0.43657534234503004 1102 24-25 0.3889734986185758 1102 26-27 0.4041808761911625 1102 28-29 0.4110877309897134 1102 30-31 0.43556629477919273 1102 32-33 0.40633564773385444 1102 34-35 0.44854645355302125 1102 36-37 0.4467807875844798 1102 38-39 0.4578597427767761 1102 40-41 0.44392308807673686 1102 42-43 0.44001941421917934 1102 44-45 0.42118355068046043 1102 46-47 0.37123483616437625 1102 48-49 0.3812796408331991 1102 50-51 0.5073510135405854 1102 52-53 0.3428683586084844 1102 54-55 0.421923670381382 1102 56-57 0.43539454628805885 1102 58-59 0.4598291465759061 1102 60-61 0.375499863181723 1102 62-63 0.35617434844306217 1102 64-65 0.39170446772530454 1102 66-67 0.35846573048429065 1102 68-69 0.36290911778630885 1102 70-71 0.3647288000304556 1102 72-73 0.41552778328663464 1102 74-75 0.4183722858872798 1102 76-77 0.41955063176505547 1102 78-79 0.393830486932373 1102 80-81 0.3911439578189544 1102 82-83 0.4076011925742762 1102 84-85 0.389815760322648 1102 86-87 0.39091849285443914 1102 88-89 0.3869460287188531 1102 90-91 0.3566085530549401 1102 92-93 0.40357336962211576 1102 94-95 0.3948179621775836 1102 96-97 0.3959227067773625 1102 98-99 0.4120112137301746 1102 100-101 0.43040103151299647 1103 1 0.14655717170791505 1103 2 0.23452891549030142 1103 3 0.2568174310648672 1103 4 0.2445085111818841 1103 5 0.2533406046150972 1103 6 0.22952293646888222 1103 7 0.29479715690911945 1103 8 0.268100214084086 1103 9 0.359743295758598 1103 10-11 0.3356579497627692 1103 12-13 0.3552969545539426 1103 14-15 0.44605950775574144 1103 16-17 0.45151861923057623 1103 18-19 0.4396984460928124 1103 20-21 0.41859765151049544 1103 22-23 0.40229281182896415 1103 24-25 0.431541473436063 1103 26-27 0.39562448686768903 1103 28-29 0.4561562755836306 1103 30-31 0.35861618855400934 1103 32-33 0.41220650935583336 1103 34-35 0.40181851384507894 1103 36-37 0.39195810944854514 1103 38-39 0.36302702191387937 1103 40-41 0.37249275878659205 1103 42-43 0.3493466891972332 1103 44-45 0.30551932753738953 1103 46-47 0.36022817603428336 1103 48-49 0.2825653724347674 1103 50-51 0.4270769262477998 1103 52-53 0.32396110584987525 1103 54-55 0.3539439273541092 1103 56-57 0.32895763953214896 1103 58-59 0.33367136717920687 1103 60-61 0.31465783402047265 1103 62-63 0.3097726556528073 1103 64-65 0.31693696803677796 1103 66-67 0.28318299133540137 1103 68-69 0.2332975288541448 1103 70-71 0.27407261141384254 1103 72-73 0.27069926815333645 1103 74-75 0.29379606111907464 1103 76-77 0.28062717412507965 1103 78-79 0.3199612301906427 1103 80-81 0.2969048673785508 1103 82-83 0.26747342257936424 1103 84-85 0.2976048246244787 1103 86-87 0.33591851482928803 1103 88-89 0.3488753835681564 1103 90-91 0.3132395861557242 1103 92-93 0.3182754273266184 1103 94-95 0.3405830228170643 1103 96-97 0.30028715448923293 1103 98-99 0.3387291342335246 1103 100-101 0.23564563337812316 1104 1 0.15204666889120588 1104 2 0.2050677921218167 1104 3 0.2173801144232499 1104 4 0.1735103583913613 1104 5 0.16571750124961682 1104 6 0.24561814748872735 1104 7 0.21893691089228895 1104 8 0.24016986752545222 1104 9 0.2983488527049758 1104 10-11 0.2763654732834553 1104 12-13 0.3350221578922117 1104 14-15 0.4264982491739957 1104 16-17 0.40739608318748566 1104 18-19 0.4197989028473401 1104 20-21 0.38144245470142835 1104 22-23 0.341316398546347 1104 24-25 0.3830109085089646 1104 26-27 0.3466033657354899 1104 28-29 0.35413142273056764 1104 30-31 0.3467977310900068 1104 32-33 0.31734693110220746 1104 34-35 0.34098659161536204 1104 36-37 0.38682490768190547 1104 38-39 0.3675132803104191 1104 40-41 0.33269834320181246 1104 42-43 0.2751322876006128 1104 44-45 0.2686942429394392 1104 46-47 0.2758714667557527 1104 48-49 0.320165507354055 1104 50-51 0.4410168570355495 1104 52-53 0.3432217148594816 1104 54-55 0.29309021191739504 1104 56-57 0.2811252150265062 1104 58-59 0.29711584286319237 1104 60-61 0.2858526038702891 1104 62-63 0.23066580973884498 1104 64-65 0.27684440491770346 1104 66-67 0.22904432740453728 1104 68-69 0.20101387160751472 1104 70-71 0.21214302623251768 1104 72-73 0.25436898316444 1104 74-75 0.265096543701425 1104 76-77 0.2160916087283553 1104 78-79 0.24575693473648386 1104 80-81 0.25012964205829036 1104 82-83 0.20082882872079466 1104 84-85 0.2626512708742794 1104 86-87 0.23598876264509272 1104 88-89 0.23661783609910714 1104 90-91 0.24290005726710717 1104 92-93 0.23003400703979793 1104 94-95 0.25180302418345235 1104 96-97 0.2492325821169672 1104 98-99 0.23631399613886828 1104 100-101 0.22710796815102086 1105 1 0.0699666901470124 1105 2 0.1595684821606369 1105 3 0.16786876242547777 1105 4 0.179655888200962 1105 5 0.15607677679118126 1105 6 0.18493018112017268 1105 7 0.12921545057407258 1105 8 0.1755712114669592 1105 9 0.26360340770880697 1105 10-11 0.2593681054435635 1105 12-13 0.2764899642874141 1105 14-15 0.3631357512071105 1105 16-17 0.3756418958729526 1105 18-19 0.3094589463573456 1105 20-21 0.31620279202104484 1105 22-23 0.32738404544469546 1105 24-25 0.33059082375137194 1105 26-27 0.32254737059935934 1105 28-29 0.30233767277280066 1105 30-31 0.3318409142613845 1105 32-33 0.3382428871514094 1105 34-35 0.3115775407725181 1105 36-37 0.32905765160332834 1105 38-39 0.3122900490269487 1105 40-41 0.30966725540487516 1105 42-43 0.1997485832469721 1105 44-45 0.22865019563753464 1105 46-47 0.22713759085334573 1105 48-49 0.216670735441582 1105 50-51 0.3218357740805118 1105 52-53 0.2488741031359325 1105 54-55 0.22211157698684758 1105 56-57 0.19171958704342273 1105 58-59 0.2122772680034828 1105 60-61 0.2101254131780692 1105 62-63 0.18188022039334584 1105 64-65 0.18805867283345634 1105 66-67 0.23226118799889406 1105 68-69 0.2367228815049458 1105 70-71 0.25444021265848704 1105 72-73 0.22840927181821513 1105 74-75 0.23431810757700333 1105 76-77 0.2591955300856128 1105 78-79 0.27477830111068613 1105 80-81 0.24493107430993888 1105 82-83 0.22493436490485408 1105 84-85 0.20725653127505694 1105 86-87 0.2571267352594475 1105 88-89 0.24888653244306624 1105 90-91 0.2589054103097652 1105 92-93 0.2731853296683511 1105 94-95 0.2924725321081567 1105 96-97 0.2636369060105679 1105 98-99 0.22366494145173021 1105 100-101 0.2156991945397273 1106 1 0.06688475838552321 1106 2 0.14178252953329462 1106 3 0.14319358040850005 1106 4 0.1335770205623703 1106 5 0.05043776818926915 1106 6 0.1134008060990439 1106 7 0.12958040439371388 1106 8 0.18144071694877795 1106 9 0.19216223263273946 1106 10-11 0.2324473303085668 1106 12-13 0.24751267450668735 1106 14-15 0.24897589739128279 1106 16-17 0.27680909177767177 1106 18-19 0.18971082513515825 1106 20-21 0.1764649861240173 1106 22-23 0.24855237640876027 1106 24-25 0.22508119170071694 1106 26-27 0.2250358449237666 1106 28-29 0.25751595500377533 1106 30-31 0.24761305002317613 1106 32-33 0.23327789679800048 1106 34-35 0.18490311394359082 1106 36-37 0.19511878739395883 1106 38-39 0.1904490086125179 1106 40-41 0.16205371511920674 1106 42-43 0.15648881306885443 1106 44-45 0.13278714791670865 1106 46-47 0.18555525083128543 1106 48-49 0.16275201341810686 1106 50-51 0.27029681297658215 1106 52-53 0.22537615498086438 1106 54-55 0.21886992835357688 1106 56-57 0.17908227102583396 1106 58-59 0.18354042990063846 1106 60-61 0.22144344659717063 1106 62-63 0.22694092495866158 1106 64-65 0.2614694739670895 1106 66-67 0.15923362291893994 1106 68-69 0.18333979439582038 1106 70-71 0.15692449064086844 1106 72-73 0.1625146566026956 1106 74-75 0.19072060223070508 1106 76-77 0.22069992586903453 1106 78-79 0.15343832696373028 1106 80-81 0.16505753542698187 1106 82-83 0.17744145793829347 1106 84-85 0.18198745811369577 1106 86-87 0.1750377324825223 1106 88-89 0.15268206496104142 1106 90-91 0.10394218165239266 1106 92-93 0.14883141601519867 1106 94-95 0.14869120523145796 1106 96-97 0.12129786700820233 1106 98-99 0.09626706781499905 1106 100-101 0.07803647338130659 1107 1 0.0899935230074469 1107 2 0.08229438757213359 1107 3 0.1324500087013476 1107 4 0.15913728208236222 1107 5 0.12561605831761824 1107 6 0.061779502642878015 1107 7 0.11044665444786972 1107 8 0.1042904965483018 1107 9 0.141772281229251 1107 10-11 0.08388821304567529 1107 12-13 0.10004234550518021 1107 14-15 0.2062804307273396 1107 16-17 0.18030636974491188 1107 18-19 0.17652343638568624 1107 20-21 0.13175881523157784 1107 22-23 0.21762179462491105 1107 24-25 0.15636533458502555 1107 26-27 0.14588156512957084 1107 28-29 0.12518673900463995 1107 30-31 0.1788095369797702 1107 32-33 0.11562582080154016 1107 34-35 0.1440945443672348 1107 36-37 0.20333304311481726 1107 38-39 0.1548292890696672 1107 40-41 0.16382242315984996 1107 42-43 0.18999859471067992 1107 44-45 0.23011552275428215 1107 46-47 0.1560511411872838 1107 48-49 0.04292619047653545 1107 50-51 0.15578065806879948 1107 52-53 0.09541296591642379 1107 54-55 0.1426662491396229 1107 56-57 0.1315778442928064 1107 58-59 0.11653120440664111 1107 60-61 0.1258825336349716 1107 62-63 0.14492645573781715 1107 64-65 0.12516365970976295 1107 66-67 0.08661176632685397 1107 68-69 0.11936760408501357 1107 70-71 0.09725586999947922 1107 72-73 0.1528097512304214 1107 74-75 0.12082178443375113 1107 76-77 0.10518721342037196 1107 78-79 0.103972440230919 1107 80-81 0.11129672766016796 1107 82-83 0.13938226016112054 1107 84-85 0.1627135311349619 1107 86-87 0.15343846524426397 1107 88-89 0.0758364719386293 1107 90-91 0.1346979835815887 1107 92-93 0.12386619427302747 1107 94-95 0.1457476966150395 1107 96-97 0.12399019259044763 1107 98-99 0.11054280183687837 1107 100-101 0.11897432121952178 1108 1 0.052216994923725224 1108 2 0.1487388563294516 1108 3 0.15701891863820805 1108 4 0.15690637010400366 1108 5 0.1084216346267155 1108 6 0.17567054931011228 1108 7 0.16093088477133932 1108 8 0.1312971111664254 1108 9 0.16138640735901788 1108 10-11 0.21993676097086023 1108 12-13 0.17101348034247366 1108 14-15 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72-73 -0.4219743775280733 2214 74-75 -0.3807320892715964 2214 76-77 -0.4452359740596705 2214 78-79 -0.5051172504201702 2214 80-81 -0.43081812610630976 2214 82-83 -0.5151652942486962 2214 84-85 -0.4533578974364687 2214 86-87 -0.5250092009509331 2214 88-89 -0.4300279825943818 2214 90-91 -0.3772288312197034 2214 92-93 -0.3406852954381563 2214 94-95 -0.3354044911843843 2214 96-97 -0.3798872199203842 2214 98-99 -0.47433908208883935 2214 100-101 -0.48506335501354414 2215 1 -0.25409511235945814 2215 2 -0.2857140298465026 2215 3 -0.24249098640572342 2215 4 -0.31036950738729274 2215 5 -0.2213958406183707 2215 6 -0.25619991892097715 2215 7 -0.33567731786638433 2215 8 -0.3282924534825611 2215 9 -0.4628613963296786 2215 10-11 -0.44075890004678087 2215 12-13 -0.44548841218320234 2215 14-15 -0.5970259596844798 2215 16-17 -0.5244094037486846 2215 18-19 -0.5737872449490666 2215 20-21 -0.627240821448801 2215 22-23 -0.5264353228496077 2215 24-25 -0.5028928336832124 2215 26-27 -0.5410131019385531 2215 28-29 -0.5891773964758968 2215 30-31 -0.5946757084226846 2215 32-33 -0.6160164635829233 2215 34-35 -0.6003257037310163 2215 36-37 -0.5753766785226588 2215 38-39 -0.5602822164623333 2215 40-41 -0.5526826075518656 2215 42-43 -0.4770078097671231 2215 44-45 -0.4808524595280872 2215 46-47 -0.4836694783806337 2215 48-49 -0.4733152253463402 2215 50-51 -0.3754765898919672 2215 52-53 -0.5440366589721606 2215 54-55 -0.4214212226488172 2215 56-57 -0.33642868494072786 2215 58-59 -0.4350571506298522 2215 60-61 -0.40100807142815853 2215 62-63 -0.3872336585075402 2215 64-65 -0.40346485837891777 2215 66-67 -0.38724411429905103 2215 68-69 -0.3876253384710253 2215 70-71 -0.4163944477121859 2215 72-73 -0.4278453502222703 2215 74-75 -0.44434858558081913 2215 76-77 -0.42612555146155984 2215 78-79 -0.35835637167468803 2215 80-81 -0.3960412677965266 2215 82-83 -0.35569603827493523 2215 84-85 -0.3346286406163088 2215 86-87 -0.325878973849683 2215 88-89 -0.3467572389643294 2215 90-91 -0.37332861818286034 2215 92-93 -0.3460603339306587 2215 94-95 -0.379413730284476 2215 96-97 -0.34560891825444884 2215 98-99 -0.40760025886980245 2215 100-101 -0.3295036313131483 2216 1 -0.32446115132612263 2216 2 -0.39773534700993807 2216 3 -0.3511989337991217 2216 4 -0.2771357930817331 2216 5 -0.21361324860349384 2216 6 -0.21668115133137178 2216 7 -0.2869879582507764 2216 8 -0.29915143553477463 2216 9 -0.39375054736041193 2216 10-11 -0.4379887440241461 2216 12-13 -0.5044382585299871 2216 14-15 -0.6687444201175268 2216 16-17 -0.7290763942988718 2216 18-19 -0.6146219490196501 2216 20-21 -0.5981691925824038 2216 22-23 -0.6959643003417639 2216 24-25 -0.666724176327719 2216 26-27 -0.6269385602115563 2216 28-29 -0.628174624122579 2216 30-31 -0.685640296319022 2216 32-33 -0.701063513523394 2216 34-35 -0.75867628867978 2216 36-37 -0.8203606190113462 2216 38-39 -0.7940275190092407 2216 40-41 -0.7030489219209386 2216 42-43 -0.7172502562645278 2216 44-45 -0.6501843684392483 2216 46-47 -0.6062632489439181 2216 48-49 -0.6409112913734276 2216 50-51 -0.6010408614081939 2216 52-53 -0.6146048850677204 2216 54-55 -0.5967944450382348 2216 56-57 -0.5917614646990685 2216 58-59 -0.5288549026467919 2216 60-61 -0.5577080682777549 2216 62-63 -0.5334819575065382 2216 64-65 -0.6273179957464734 2216 66-67 -0.5980907417741577 2216 68-69 -0.5429767804370158 2216 70-71 -0.48346147636519277 2216 72-73 -0.4913578390438431 2216 74-75 -0.5321718928171251 2216 76-77 -0.4933563856131755 2216 78-79 -0.5039891630465405 2216 80-81 -0.5282566265077264 2216 82-83 -0.4834699946922356 2216 84-85 -0.4797203183112657 2216 86-87 -0.4443374588050446 2216 88-89 -0.4692501926818551 2216 90-91 -0.47724268499740674 2216 92-93 -0.5168558902640541 2216 94-95 -0.5568817700011621 2216 96-97 -0.5604499052393557 2216 98-99 -0.6405009815701739 2216 100-101 -0.6257436289009988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 184.0 11 1724.0 12 6147.0 13 11740.0 14 16291.0 15 19006.0 16 20190.0 17 20786.0 18 21906.0 19 23797.0 20 28482.0 21 46027.0 22 68218.0 23 83442.0 24 89173.0 25 87610.0 26 87378.0 27 94082.0 28 113004.0 29 136952.0 30 152543.0 31 179217.0 32 234587.0 33 318677.0 34 458522.0 35 760021.0 36 1088017.0 37 2278397.0 38 3183668.0 39 731543.0 40 420.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.356731178279503 29.081143806568623 27.775457277881195 22.786667737270687 2 23.055512233838446 29.17399489970422 26.08140978475455 21.689083081702783 3 22.815350145419426 28.011701110005333 25.840987122641035 23.331961621934205 4 23.638521748059592 27.172248476898503 25.841363506866404 23.347866268175498 5 23.94534244787947 27.952714946275492 25.826047564157108 22.27589504168793 6 23.839124889304433 27.82731144308414 24.796588453381244 23.53697521423018 7 24.050855492391634 28.14998853475744 25.313576314121395 22.485579658729527 8 23.437378157670633 28.21969682347155 25.37489799022405 22.968027028633767 9 22.80692492929767 27.99726339715523 25.69605024324072 23.499761430306382 10-11 22.992602988374937 28.240277319897256 25.47698497319758 23.290134718530226 12-13 22.9418055942663 28.39422329351253 25.55578921402481 23.108181898196367 14-15 22.740269986609242 28.636058533289905 25.70444307722003 22.919228402880822 16-17 22.608734507446233 28.601972909600615 25.837035088274646 22.952257494678506 18-19 22.42036385352593 28.49858305815464 26.080208250496632 23.000844837822793 20-21 22.364133015343352 28.587178114280288 26.143773755635145 22.904915114741215 22-23 22.42135157530401 28.38039071793499 26.535667830498838 22.662589876262164 24-25 22.41900307978805 28.154094983165006 26.78884806353211 22.638053873514828 26-27 22.444650286939893 28.170279504855934 26.85361510292854 22.531455105275633 28-29 22.403938059895662 28.261933889172408 26.846352817554408 22.487775233377523 30-31 22.392024051531052 28.198339431401177 26.890394597361528 22.519241919706243 32-33 22.41069849963597 28.212381458270762 26.894713365075713 22.482206677017555 34-35 22.438058737557125 28.217380612853887 26.919125259898134 22.425435389690858 36-37 22.442872153439517 28.23076728203161 26.819343763533865 22.507016800995004 38-39 22.502363535501672 28.253707247416326 26.706672408381877 22.537256808700125 40-41 22.514264899633798 28.36682010187694 26.612173624834334 22.50674137365493 42-43 22.51352505591708 28.374686635155257 26.584958970015897 22.526829338911767 44-45 22.510057831718083 28.39490695615966 26.589913828341803 22.505121383780455 46-47 22.505851967692923 28.464047103930472 26.579884094195698 22.450216834180907 48-49 22.513351878151404 28.413656600005915 26.567041872148074 22.505949649694603 50-51 22.582103082340545 28.40209449489603 26.595536671370233 22.420265751393192 52-53 22.513691602274818 28.443878449708055 26.599369186866063 22.443060761151067 54-55 22.49482087439758 28.416574939160654 26.591249847388916 22.49735433905285 56-57 22.475293614892745 28.44953376335423 26.58023463134031 22.494937990412716 58-59 22.497512968849286 28.500638695077825 26.594937805884566 22.40691053018833 60-61 22.466837654481598 28.4762991818372 26.560735095763725 22.49612806791747 62-63 22.485507277147725 28.49166820437316 26.569541521549638 22.453282996929477 64-65 22.483871453399 28.522618568751696 26.586382302915567 22.40712767493374 66-67 22.4639048716812 28.5174229472691 26.5782961500283 22.440376031021398 68-69 22.479446526031506 28.544762507344316 26.53977821511266 22.43601275151152 70-71 22.473585786803792 28.579098262446184 26.564337436790364 22.38297851395966 72-73 22.45429772495695 28.55881899349318 26.54196139682358 22.444921884726288 74-75 22.47134981722136 28.573375987795067 26.562907449481816 22.392366745501757 76-77 22.46650762829725 28.642256366396452 26.520991998330768 22.37024400697553 78-79 22.461438537383568 28.606107778594353 26.54764346215222 22.384810221869856 80-81 22.46395148422632 28.64172066621365 26.529067106132885 22.36526074342714 82-83 22.454342666078794 28.69074055192162 26.53657535240095 22.318341429598636 84-85 22.41348518751911 28.690298905565285 26.526232476187072 22.36998343072853 86-87 22.421418761828566 28.744553190298383 26.52914085413697 22.304887193736082 88-89 22.420453803541793 28.786345099402073 26.521371116533093 22.27182998052304 90-91 22.406892052691976 28.793569882997495 26.480494281996858 22.319043782313674 92-93 22.40155861702898 28.82143460322822 26.51024199580187 22.266764783940936 94-95 22.405955093308545 28.899832769593402 26.46094502165271 22.23326711544534 96-97 22.384901703881738 28.936670169291833 26.432513536320883 22.245914590505546 98-99 22.365291120652184 28.997094313780142 26.412425234651437 22.22518933091624 100-101 22.39813788247393 29.064968935755264 26.351045653283343 22.18584752848746 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 186170.0 1 98091.5 2 7453.5 3 4200.5 4 3112.0 5 2441.5 6 1919.0 7 1515.0 8 1300.5 9 1149.5 10 1088.5 11 1071.5 12 1073.5 13 1462.5 14 2076.0 15 2973.5 16 9642.0 17 9256.0 18 2989.5 19 3691.0 20 5151.5 21 7496.5 22 10557.0 23 14549.5 24 19682.5 25 26460.5 26 35373.0 27 45601.5 28 57156.5 29 70283.5 30 85768.0 31 103111.0 32 120701.5 33 140121.5 34 160530.0 35 183906.0 36 212928.0 37 243254.5 38 274893.5 39 306846.5 40 335776.0 41 361055.0 42 385998.5 43 409878.0 44 427595.5 45 439804.5 46 448087.5 47 451799.0 48 448169.5 49 437380.5 50 423606.5 51 410095.0 52 395533.5 53 374885.5 54 347136.0 55 313271.5 56 276184.0 57 240593.5 58 208658.5 59 177119.5 60 143801.0 61 115198.0 62 92696.0 63 73400.0 64 57412.0 65 44064.0 66 33278.0 67 25342.0 68 19679.5 69 15774.5 70 12788.5 71 10622.0 72 8866.0 73 7101.0 74 5315.0 75 3632.5 76 2478.0 77 1688.0 78 1026.5 79 545.5 80 218.0 81 75.5 82 33.5 83 18.0 84 10.5 85 5.0 86 4.0 87 3.0 88 1.5 89 0.0 90 1.0 91 1.5 92 1.5 93 2.0 94 1.0 95 0.5 96 3.0 97 3.5 98 4.0 99 5.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012256614518471394 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.790526544159714E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.3993772481719308E-4 38-39 0.0015055369014815255 40-41 0.008864331051351163 42-43 0.004120924723926996 44-45 4.873693174667759E-4 46-47 0.004762708082571365 48-49 7.238158180199642E-5 50-51 0.001090549165816746 52-53 0.00809708628425 54-55 0.004313942275398986 56-57 7.238158180199642E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.825438786799761E-6 68-69 0.0 70-71 9.650877573599522E-6 72-73 1.3511228603039333E-4 74-75 3.667333477967818E-4 76-77 9.795640737203515E-4 78-79 4.535912459591776E-4 80-81 8.782298591975565E-4 82-83 7.86546522248361E-4 84-85 9.795640737203515E-4 86-87 0.001756459718395113 88-89 2.0749386783238975E-4 90-91 0.001317344788796335 92-93 1.9301755147199045E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0361752E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.94981370409977 #Duplication Level Percentage of deduplicated Percentage of total 1 82.0834265707378 54.13386690534311 2 10.105433818785714 13.329029554960547 3 3.328403717130131 6.58522815230296 4 1.471513866861052 3.8818426152994334 5 0.8083995881690141 2.6656901119108727 6 0.47821651309368446 1.8922973969251609 7 0.3199736037365931 1.4771539689660431 8 0.22940681284474043 1.210346925564953 9 0.17184595929431767 1.0199888101136307 >10 0.987979535114845 10.977956093631997 >50 0.014159641589306922 0.585417305901212 >100 0.0010500970432976424 0.10341216905490352 >500 5.858038260101166E-5 0.029560836700179276 >1k 1.0242984408597297E-4 0.1271907569497154 >5k 1.4632686403206444E-5 0.0694913020737253 >10k+ 1.4632686403206444E-5 1.9115270943015665 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198081 1.911655480656167 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.650877573599522E-6 9.650877573599522E-6 0.0 0.0 0.0 2 9.650877573599522E-6 9.650877573599522E-6 0.0 0.0 0.0 3 9.650877573599522E-6 9.650877573599522E-6 0.0 0.0 0.0 4 9.650877573599522E-6 9.650877573599522E-6 0.0 0.0 0.0 5 1.9301755147199045E-5 9.650877573599522E-6 0.0 0.0 0.0 6 2.895263272079857E-5 9.650877573599522E-6 0.0 9.650877573599522E-6 0.0 7 4.825438786799761E-5 9.650877573599522E-6 0.0 9.650877573599522E-6 0.0 8 6.755614301519665E-5 9.650877573599522E-6 0.0 9.650877573599522E-6 0.0 9 1.351122860303933E-4 9.650877573599522E-6 0.0 9.650877573599522E-6 0.0 10-11 1.9784299025879022E-4 9.650877573599522E-6 0.0 9.650877573599522E-6 0.0 12-13 3.0400264356838494E-4 9.650877573599522E-6 0.0 9.650877573599522E-6 4.825438786799761E-6 14-15 4.4394036838557805E-4 1.4476316360399284E-5 0.0 9.650877573599522E-6 9.650877573599522E-6 16-17 5.64576338055572E-4 1.9301755147199045E-5 0.0 1.9301755147199045E-5 9.650877573599522E-6 18-19 7.093395016595649E-4 1.9301755147199045E-5 0.0 1.9301755147199045E-5 9.650877573599522E-6 20-21 8.299754713295589E-4 1.9301755147199045E-5 0.0 1.9301755147199045E-5 9.650877573599522E-6 22-23 9.747386349335518E-4 1.9301755147199045E-5 0.0 1.9301755147199045E-5 9.650877573599522E-6 24-25 0.0011725816251923421 1.9301755147199045E-5 0.0 1.9301755147199045E-5 1.4476316360399284E-5 26-27 0.001317344788796335 1.9301755147199045E-5 0.0 3.377807150759833E-5 1.9301755147199045E-5 28-29 0.0015489658505627234 1.9301755147199045E-5 0.0 3.860351029439809E-5 1.9301755147199045E-5 30-31 0.0017805869123291118 1.9301755147199045E-5 0.0 5.790526544159714E-5 1.9301755147199045E-5 32-33 0.002007382535308701 3.377807150759833E-5 0.0 5.790526544159714E-5 1.9301755147199045E-5 34-35 0.0025526571182170736 3.860351029439809E-5 0.0 5.790526544159714E-5 1.9301755147199045E-5 36-37 0.005742272156291715 3.860351029439809E-5 0.0 5.790526544159714E-5 1.9301755147199045E-5 38-39 0.005906337075042908 3.860351029439809E-5 0.0 6.755614301519665E-5 1.9301755147199045E-5 40-41 0.0060655765550073 3.860351029439809E-5 0.0 7.238158180199642E-5 1.9301755147199045E-5 42-43 0.006157259891956495 3.860351029439809E-5 0.0 7.720702058879618E-5 1.9301755147199045E-5 44-45 0.006287546739200089 3.860351029439809E-5 0.0 8.68578981623957E-5 1.9301755147199045E-5 46-47 0.0064371353415908815 3.860351029439809E-5 0.0 9.650877573599522E-5 1.9301755147199045E-5 48-49 0.006630152893062872 3.860351029439809E-5 0.0 1.0615965330959475E-4 1.9301755147199045E-5 50-51 0.006794217811814064 3.860351029439809E-5 0.0 1.1581053088319428E-4 1.9301755147199045E-5 52-53 0.0069872353632860545 4.3428949081197846E-5 0.0 1.2063596966999404E-4 1.9301755147199045E-5 54-55 0.007223681863839242 5.790526544159714E-5 0.0 1.351122860303933E-4 1.9301755147199045E-5 56-57 0.007450477486818831 5.790526544159714E-5 0.0 1.351122860303933E-4 1.9301755147199045E-5 58-59 0.007701400303732419 5.790526544159714E-5 0.0 1.3993772481719305E-4 1.9301755147199045E-5 60-61 0.007947497681859206 7.720702058879618E-5 0.0 1.5441404117759236E-4 2.895263272079857E-5 62-63 0.008227373131493593 7.720702058879618E-5 0.0 1.5441404117759236E-4 2.895263272079857E-5 64-65 0.00865683718351877 7.720702058879618E-5 0.0 1.592394799643921E-4 3.860351029439809E-5 66-67 0.009057348602823152 8.68578981623957E-5 0.0 1.6406491875119189E-4 3.860351029439809E-5 68-69 0.009636401257239123 9.168333694919547E-5 0.0 1.7854123511159116E-4 3.860351029439809E-5 70-71 0.010365042514045887 9.650877573599522E-5 0.0 1.8336667389839094E-4 4.3428949081197846E-5 72-73 0.011291526761111441 1.0615965330959475E-4 0.0 1.8336667389839094E-4 4.825438786799761E-5 74-75 0.012642649621415373 1.0615965330959475E-4 0.0 1.8336667389839094E-4 4.825438786799761E-5 76-77 0.01457282513613528 1.0615965330959475E-4 0.0 1.8336667389839094E-4 4.825438786799761E-5 78-79 0.017062751550123954 1.1098509209639451E-4 0.0 1.8336667389839094E-4 5.790526544159714E-5 80-81 0.02045503501724419 1.1581053088319428E-4 0.0 1.8336667389839094E-4 5.790526544159714E-5 82-83 0.024575959741171185 1.1581053088319428E-4 0.0 1.8336667389839094E-4 6.755614301519665E-5 84-85 0.030260326632021304 1.2063596966999404E-4 0.0 1.8336667389839094E-4 6.755614301519665E-5 86-87 0.03810166466057091 1.351122860303933E-4 0.0 1.8336667389839094E-4 6.755614301519665E-5 88-89 0.0483846747152412 1.351122860303933E-4 0.0 1.9301755147199044E-4 7.720702058879618E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2965 0.0 76.57262 1 TCGACGG 3685 0.0 62.255814 2 CGACGGC 6250 0.0 36.554035 3 ATCTCGA 3375 0.0 31.3835 1 ACGGCGC 7725 0.0 29.697435 5 GACGGCG 7830 0.0 29.481174 4 CGGCGCG 8500 0.0 26.82209 6 GGGCGCG 3840 0.0 26.346243 8 TCTCGAG 4085 0.0 24.998657 2 CGCGCCG 11845 0.0 24.300793 9 GGCGCGC 12150 0.0 23.92464 7 GCGCGCC 12400 0.0 23.518898 8 TCGAGGG 4440 0.0 22.678963 4 CTCGAGG 4605 0.0 22.072649 3 AGGGCGC 4680 0.0 21.9219 7 CGAGGGC 5270 0.0 19.377518 5 GAGGGCG 6185 0.0 16.818012 6 CGCCGGA 11915 0.0 16.424269 9 TATCTCG 1725 0.0 15.694809 2 GCGCCGG 12865 0.0 15.284838 8 >>END_MODULE