FastQCFastQC Report
Tue 31 May 2016
SRR1567938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10361752
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3588543.463256020796483No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT308080.2973242362874541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA267910.2585566610743048No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT232990.22485579658729526No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201190.19416600590324878No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128410.12392691892259147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG895850.094.076231
TCGACGG917300.091.8083652
TATCTCG604100.089.871572
CGACGGC943450.089.127733
ATATCTC614550.088.8261261
ACGGCGC974600.087.068595
GACGGCG971150.087.064874
CGGCGCG980950.086.412976
ATCTCGA662800.081.8620453
TCTCGAG673300.078.14454
CTCGAGG708800.073.982715
TCGAGGG710650.073.810176
CGAGGGC726050.072.2904057
AGGGCGC743450.071.716569
GAGGGCG749900.070.8590248
GGCGCGC1734350.050.989557
CGCGCCG1724450.050.9627659
TACGGCG7000.050.8918384
GCGCGCC1737700.050.861188
CTCGACG15400.043.489395
Produced by FastQC (version 0.11.5)