FastQCFastQC Report
Tue 31 May 2016
SRR1567937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12514508
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2088341.6687351991784256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG36650.079.0551151
TCGACGG47550.061.8321342
ATCTCGA45450.037.7265781
CGACGGC78400.037.6226733
TCTCGAG55050.030.112182
ACGGCGC100050.029.5289085
GACGGCG100000.029.4961744
GGGCGCG54800.029.4694798
TCGAGGG57650.028.1773994
CGGCGCG110450.026.877476
CTCGAGG62100.026.1582453
AGGGCGC65150.025.0065527
TATCTCG22700.024.8997612
CGAGGGC67350.023.9781365
CGCGCCG159350.022.9520479
GGCGCGC164250.022.4403927
GCGCGCC166450.022.2579388
GAGGGCG79250.020.737246
CGCCGGA158900.016.351079
GCGCCGG171500.015.2325538
Produced by FastQC (version 0.11.5)