##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567937_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12514508 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.719847236503426 31.0 31.0 34.0 29.0 34.0 2 31.818458464367914 33.0 31.0 34.0 28.0 34.0 3 31.837599368668748 34.0 31.0 34.0 28.0 34.0 4 35.369339010371 37.0 35.0 37.0 32.0 37.0 5 35.26967068941104 37.0 35.0 37.0 32.0 37.0 6 35.249833233555805 37.0 35.0 37.0 32.0 37.0 7 35.17079424936242 37.0 35.0 37.0 32.0 37.0 8 35.11900995228898 37.0 35.0 37.0 32.0 37.0 9 36.63872059532824 39.0 37.0 39.0 32.0 39.0 10-11 36.63320383829712 39.0 37.0 39.0 32.0 39.0 12-13 36.53146280301232 39.0 37.0 39.0 32.0 39.0 14-15 37.79588873969317 40.0 38.0 41.0 32.0 41.0 16-17 37.80717100504471 40.0 38.0 41.0 32.0 41.0 18-19 37.83859685095091 40.0 38.0 41.0 32.0 41.0 20-21 37.865274128235804 40.0 38.0 41.0 32.0 41.0 22-23 37.90370824006824 40.0 38.0 41.0 32.0 41.0 24-25 37.89463844683307 40.0 38.0 41.0 32.0 41.0 26-27 37.88662438827 40.0 38.0 41.0 32.0 41.0 28-29 37.82723304024417 40.0 37.5 41.0 32.0 41.0 30-31 37.80483823255376 40.0 37.0 41.0 32.0 41.0 32-33 37.7702925676343 40.0 37.0 41.0 32.0 41.0 34-35 37.74744740264659 40.0 37.0 41.0 32.0 41.0 36-37 37.714565406806244 40.0 37.0 41.0 31.5 41.0 38-39 37.67699856838159 40.0 37.0 41.0 31.0 41.0 40-41 37.60997623718008 40.0 37.0 41.0 31.0 41.0 42-43 37.5405515742209 40.0 37.0 41.0 31.0 41.0 44-45 37.47980336102706 40.0 36.0 41.0 31.0 41.0 46-47 37.42697931073279 40.0 36.0 41.0 31.0 41.0 48-49 37.35281187242839 40.0 36.0 41.0 30.5 41.0 50-51 37.152325045459236 39.5 35.0 41.0 30.0 41.0 52-53 37.0848949475281 39.0 35.0 41.0 30.0 41.0 54-55 37.23628415915352 39.0 35.5 41.0 30.5 41.0 56-57 37.28838504877699 39.0 35.5 41.0 31.0 41.0 58-59 37.15559972473548 39.0 35.0 41.0 31.0 41.0 60-61 36.97733398708124 39.0 35.0 41.0 31.0 41.0 62-63 36.72778370512049 39.0 35.0 40.5 31.0 41.0 64-65 36.43439941865873 38.0 35.0 40.0 30.0 41.0 66-67 36.154646551027014 37.5 35.0 40.0 30.0 41.0 68-69 35.799476495600146 37.0 35.0 40.0 30.0 41.0 70-71 35.45344743077395 36.5 34.0 39.0 30.0 41.0 72-73 35.06562179671786 36.0 34.0 39.0 29.5 40.5 74-75 34.69438718645591 35.5 34.0 38.5 29.0 40.0 76-77 34.33575802580493 35.0 34.0 37.0 29.0 39.0 78-79 33.9985154430362 35.0 34.0 37.0 29.0 39.0 80-81 33.68776083726184 35.0 34.0 36.5 29.0 38.5 82-83 33.39911984554247 35.0 33.5 36.0 29.0 37.0 84-85 33.144132114502625 35.0 33.0 36.0 29.0 37.0 86-87 32.96841230194587 35.0 33.0 35.0 29.0 36.5 88-89 32.80665564319428 35.0 33.0 35.0 28.5 36.0 90-91 32.647526335034506 35.0 33.0 35.0 28.0 36.0 92-93 32.48280100184522 35.0 33.0 35.0 28.0 36.0 94-95 32.37727871523195 35.0 33.0 35.0 28.0 35.5 96-97 32.25432557955934 35.0 33.0 35.0 28.0 35.0 98-99 32.11854405303029 35.0 33.0 35.0 27.5 35.0 100-101 31.70092759539568 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3471470764172828 1101 2 0.36843025775241145 1101 3 0.3661945057901548 1101 4 0.35000388794814086 1101 5 0.39576231070681445 1101 6 0.42414463401615876 1101 7 0.3756284216974066 1101 8 0.42130773579137326 1101 9 0.5076128426326179 1101 10-11 0.49224592911373577 1101 12-13 0.44931946467056605 1101 14-15 0.513783666728024 1101 16-17 0.48669183467455923 1101 18-19 0.5000783493768495 1101 20-21 0.5111185391698001 1101 22-23 0.49313001900067377 1101 24-25 0.45313560540638065 1101 26-27 0.4956777097477527 1101 28-29 0.49028303358746683 1101 30-31 0.44078425750523564 1101 32-33 0.4087841869941755 1101 34-35 0.47748872988748303 1101 36-37 0.4613247468719166 1101 38-39 0.4746962640556518 1101 40-41 0.4332915539975559 1101 42-43 0.4964353772828147 1101 44-45 0.44375609796122006 1101 46-47 0.4399616711885699 1101 48-49 0.45045071864609554 1101 50-51 0.534973448559974 1101 52-53 0.4321650421868384 1101 54-55 0.40173551642168803 1101 56-57 0.4499111857047069 1101 58-59 0.4572984954598738 1101 60-61 0.42301475518407017 1101 62-63 0.4181953414979631 1101 64-65 0.4405790372869802 1101 66-67 0.4465678222162097 1101 68-69 0.4755259374503922 1101 70-71 0.4451087105194844 1101 72-73 0.4403034461620976 1101 74-75 0.4113354512282612 1101 76-77 0.415127113753023 1101 78-79 0.42105263430458706 1101 80-81 0.3698093993519507 1101 82-83 0.3729242395406871 1101 84-85 0.3581926677313234 1101 86-87 0.3559567745393153 1101 88-89 0.3240357146070636 1101 90-91 0.32458118255603097 1101 92-93 0.30199862682984246 1101 94-95 0.3330313899821178 1101 96-97 0.34549447592274873 1101 98-99 0.3636369933662209 1101 100-101 0.3563762612113166 1102 1 0.2628378144983827 1102 2 0.2929202361837575 1102 3 0.3358412413171976 1102 4 0.3273730075777763 1102 5 0.3246537709811008 1102 6 0.373821830892922 1102 7 0.3118835330722476 1102 8 0.3587719670840386 1102 9 0.38912805025286445 1102 10-11 0.41493631650993734 1102 12-13 0.36446550138083467 1102 14-15 0.5385189633740666 1102 16-17 0.5376762072644894 1102 18-19 0.4889383446439126 1102 20-21 0.5060478071240979 1102 22-23 0.4740996243330784 1102 24-25 0.4002756017191942 1102 26-27 0.4538979064588631 1102 28-29 0.4668401265788802 1102 30-31 0.45688764844062035 1102 32-33 0.41212850503566045 1102 34-35 0.45807663906466445 1102 36-37 0.41405543858574134 1102 38-39 0.40087668026449563 1102 40-41 0.4147489162971283 1102 42-43 0.42144944233040604 1102 44-45 0.4212073851907334 1102 46-47 0.35887891675564276 1102 48-49 0.33735228773630865 1102 50-51 0.4975861659969283 1102 52-53 0.3379080288354288 1102 54-55 0.3685390201679368 1102 56-57 0.38510518827845175 1102 58-59 0.3974923735452336 1102 60-61 0.3667611434361362 1102 62-63 0.3579955613210899 1102 64-65 0.3748081053458989 1102 66-67 0.32505665396558925 1102 68-69 0.3540350646341466 1102 70-71 0.310658107710978 1102 72-73 0.3721977766197142 1102 74-75 0.3321118131474705 1102 76-77 0.331685223174766 1102 78-79 0.3027411769941537 1102 80-81 0.3287770750664478 1102 82-83 0.3686249045119965 1102 84-85 0.36134382494884676 1102 86-87 0.34132637362048257 1102 88-89 0.3630307821670158 1102 90-91 0.292928188731139 1102 92-93 0.34153754320667673 1102 94-95 0.34451778738613825 1102 96-97 0.32192237133804014 1102 98-99 0.31496040372179124 1102 100-101 0.29970017022723283 1103 1 0.1563214668501054 1103 2 0.20939043049078876 1103 3 0.27240997258068944 1103 4 0.2574259766273883 1103 5 0.2687457271148048 1103 6 0.23001566800405726 1103 7 0.25393503551662633 1103 8 0.29014620451890494 1103 9 0.3378301918799309 1103 10-11 0.3323254725440208 1103 12-13 0.28525157919344224 1103 14-15 0.38749069386343393 1103 16-17 0.3935261216057455 1103 18-19 0.39120133766098775 1103 20-21 0.3719113437998729 1103 22-23 0.36692595320351984 1103 24-25 0.3953253840787383 1103 26-27 0.398627162253419 1103 28-29 0.4202205273248083 1103 30-31 0.34466423428730764 1103 32-33 0.3340341693872375 1103 34-35 0.34088815662871497 1103 36-37 0.38637532662220764 1103 38-39 0.3717662980430916 1103 40-41 0.3476646166390509 1103 42-43 0.30459419419077705 1103 44-45 0.2869404003701277 1103 46-47 0.30312920252004005 1103 48-49 0.250962762301711 1103 50-51 0.36273913626404664 1103 52-53 0.2644544176145871 1103 54-55 0.29946927556289893 1103 56-57 0.30981724046316117 1103 58-59 0.3289303414064335 1103 60-61 0.30303302294248624 1103 62-63 0.28764520196377674 1103 64-65 0.3120727778969723 1103 66-67 0.2694411033156854 1103 68-69 0.24538519211279208 1103 70-71 0.2887247754470579 1103 72-73 0.3040762685303733 1103 74-75 0.30539439866587514 1103 76-77 0.2746757694646007 1103 78-79 0.2733602752906563 1103 80-81 0.27378570396458457 1103 82-83 0.24571421497311263 1103 84-85 0.2603464607291883 1103 86-87 0.2932983090855359 1103 88-89 0.2847942761662239 1103 90-91 0.28324264745873506 1103 92-93 0.296952854731785 1103 94-95 0.28792950279756724 1103 96-97 0.24418086884481482 1103 98-99 0.27169137628740003 1103 100-101 0.2180594183811948 1104 1 0.10718598089621878 1104 2 0.11924776210414478 1104 3 0.12502349334574703 1104 4 0.19147400114441382 1104 5 0.1768732847268737 1104 6 0.22047206707259193 1104 7 0.18508939264307855 1104 8 0.20996775596957917 1104 9 0.1982885404752608 1104 10-11 0.2507271727883733 1104 12-13 0.29927800132502114 1104 14-15 0.4034770555726297 1104 16-17 0.39122619752513543 1104 18-19 0.3749074805372885 1104 20-21 0.36623415764567824 1104 22-23 0.3680752354260619 1104 24-25 0.36100081937323836 1104 26-27 0.31840233334486356 1104 28-29 0.38654893703725435 1104 30-31 0.35739881210023583 1104 32-33 0.35022587689660867 1104 34-35 0.3500374511560338 1104 36-37 0.3505893321785507 1104 38-39 0.34969465534710054 1104 40-41 0.3201332553674803 1104 42-43 0.2742112673462245 1104 44-45 0.2818450641178387 1104 46-47 0.2656590521914808 1104 48-49 0.2858272883380266 1104 50-51 0.37984861288320104 1104 52-53 0.2775507023389565 1104 54-55 0.2559436276764515 1104 56-57 0.2433659596253861 1104 58-59 0.24769189891343046 1104 60-61 0.20883508806643647 1104 62-63 0.17723303711741778 1104 64-65 0.22403019287791892 1104 66-67 0.24490007211049658 1104 68-69 0.19762555145294414 1104 70-71 0.2400903295553647 1104 72-73 0.28524705108250714 1104 74-75 0.3068272170747335 1104 76-77 0.2309976593994918 1104 78-79 0.24959231107212076 1104 80-81 0.2662469574673665 1104 82-83 0.2605917069415611 1104 84-85 0.3200112969886604 1104 86-87 0.2935093459964051 1104 88-89 0.2479329657904472 1104 90-91 0.24949110182990353 1104 92-93 0.23960745838934372 1104 94-95 0.259165883726169 1104 96-97 0.23719894376399253 1104 98-99 0.19508652783736835 1104 100-101 0.19376681482028602 1105 1 0.10676158215853704 1105 2 0.13350258725031594 1105 3 0.1756517254917398 1105 4 0.2008013370863182 1105 5 0.17117059988552796 1105 6 0.16912884778492554 1105 7 0.18468172582645792 1105 8 0.17612718592777554 1105 9 0.2629015151988625 1105 10-11 0.25551072633976446 1105 12-13 0.31546526577812983 1105 14-15 0.43051045834759094 1105 16-17 0.3710066361710673 1105 18-19 0.3387168366744717 1105 20-21 0.3363218481637986 1105 22-23 0.34860838384459214 1105 24-25 0.3612727618781193 1105 26-27 0.34814855156442803 1105 28-29 0.322020252405828 1105 30-31 0.34540621293546536 1105 32-33 0.3697442302498004 1105 34-35 0.3475986363056549 1105 36-37 0.34041503618021096 1105 38-39 0.32131500394510937 1105 40-41 0.23162539505743496 1105 42-43 0.24066244460335184 1105 44-45 0.2555777719641412 1105 46-47 0.23253774008942685 1105 48-49 0.18658712711592784 1105 50-51 0.3013551180667591 1105 52-53 0.24502059028522183 1105 54-55 0.23422570522802033 1105 56-57 0.2094442459211976 1105 58-59 0.18716310565812933 1105 60-61 0.2219890080496043 1105 62-63 0.16532688671182427 1105 64-65 0.11724934993976888 1105 66-67 0.2050951501575753 1105 68-69 0.2202624055371416 1105 70-71 0.21000041341853404 1105 72-73 0.221687701010012 1105 74-75 0.20739931637269393 1105 76-77 0.2084857002641698 1105 78-79 0.2012723679792714 1105 80-81 0.16127524561260032 1105 82-83 0.15752599297460534 1105 84-85 0.1673655878662501 1105 86-87 0.19941524426729274 1105 88-89 0.223237977332559 1105 90-91 0.24204198782609865 1105 92-93 0.16671530965640358 1105 94-95 0.19525091352959123 1105 96-97 0.19370087731715557 1105 98-99 0.20957117896435307 1105 100-101 0.1808556382933233 1106 1 0.060123998147425084 1106 2 0.078815238058354 1106 3 0.06628041109302885 1106 4 0.11979199728712331 1106 5 0.07735008057621684 1106 6 0.09764313114513357 1106 7 0.12203644986981743 1106 8 0.11189140749346649 1106 9 0.12650001565202018 1106 10-11 0.1489950099353763 1106 12-13 0.17992788892576073 1106 14-15 0.210425402614824 1106 16-17 0.2305029520410784 1106 18-19 0.14476528357238294 1106 20-21 0.1399956790704735 1106 22-23 0.16939483672186384 1106 24-25 0.14611779006818892 1106 26-27 0.17624166118200435 1106 28-29 0.19695089542187105 1106 30-31 0.20119588795517274 1106 32-33 0.1852182677267038 1106 34-35 0.14647384375866324 1106 36-37 0.20908530654590862 1106 38-39 0.2085972077139786 1106 40-41 0.11086229167815986 1106 42-43 0.1044768433133143 1106 44-45 0.10844302167281938 1106 46-47 0.15926753034118946 1106 48-49 0.12409604318131784 1106 50-51 0.23022042368476292 1106 52-53 0.181697899340179 1106 54-55 0.15439952436431525 1106 56-57 0.14908354252308698 1106 58-59 0.18828489675736648 1106 60-61 0.19619980384522506 1106 62-63 0.17383040017125495 1106 64-65 0.19043790265342864 1106 66-67 0.10731277866073441 1106 68-69 0.15235550338434223 1106 70-71 0.1162821346016969 1106 72-73 0.12880663484867938 1106 74-75 0.14164190691636946 1106 76-77 0.15236470366580335 1106 78-79 0.13572111678752208 1106 80-81 0.1193144321614028 1106 82-83 0.15371975996809795 1106 84-85 0.13457830223435252 1106 86-87 0.12849522696482296 1106 88-89 0.12142010047431029 1106 90-91 0.082579349965485 1106 92-93 0.08368015446390586 1106 94-95 0.10116754684683826 1106 96-97 0.10240094146574563 1106 98-99 0.055887357944804705 1106 100-101 0.05929859313036445 1107 1 0.03487739973830628 1107 2 0.0037159653639662338 1107 3 0.06402514598485354 1107 4 0.10071829114713893 1107 5 0.0966179938109093 1107 6 0.07672355898950656 1107 7 0.06376258928828804 1107 8 0.06916969623065938 1107 9 0.07959680518542456 1107 10-11 0.028344730182055855 1107 12-13 0.05493025872068813 1107 14-15 0.11896957030371169 1107 16-17 0.12834462852874395 1107 18-19 0.17789703600097795 1107 20-21 0.11263983275701861 1107 22-23 0.16570393737931965 1107 24-25 0.14346365701541686 1107 26-27 0.14092389153238116 1107 28-29 0.04296069535156022 1107 30-31 0.15651541040745798 1107 32-33 0.10397561806809819 1107 34-35 0.11140993525524578 1107 36-37 0.14655288077165807 1107 38-39 0.11065369525510249 1107 40-41 0.14656403689918562 1107 42-43 0.19670372104436495 1107 44-45 0.16403698027945524 1107 46-47 0.07327706140269896 1107 48-49 0.013564922478281005 1107 50-51 0.1489094147225316 1107 52-53 0.03418531119304902 1107 54-55 0.05656677527463927 1107 56-57 0.062248234169558714 1107 58-59 0.04960322220165381 1107 60-61 0.05404210520775621 1107 62-63 0.09319497122834264 1107 64-65 0.07366853866281531 1107 66-67 0.06539374925765173 1107 68-69 0.11626891837276787 1107 70-71 0.10353384035958157 1107 72-73 0.11158736382253664 1107 74-75 0.10419813239700915 1107 76-77 0.08737141462119524 1107 78-79 0.14730965044842037 1107 80-81 0.12503071718862202 1107 82-83 0.10721391258690716 1107 84-85 0.10910029804453814 1107 86-87 0.12302722077283335 1107 88-89 0.06855079405531228 1107 90-91 0.11167989992702587 1107 92-93 0.08093778076780467 1107 94-95 0.14016885062817153 1107 96-97 0.13763562282327513 1107 98-99 0.1002917740902447 1107 100-101 0.12477080576208976 1108 1 -0.06991431305631934 1108 2 0.03768489827771404 1108 3 0.02226920923173381 1108 4 0.08875991659136417 1108 5 0.04015543301706259 1108 6 0.04691673594187051 1108 7 0.0945017894926039 1108 8 0.028233621213104243 1108 9 0.05771714989582932 1108 10-11 0.11101794795634135 1108 12-13 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-0.26852288904285615 2214 72-73 -0.3943610501283672 2214 74-75 -0.29918197089205023 2214 76-77 -0.34919272799803736 2214 78-79 -0.4285837273897073 2214 80-81 -0.34092378546512236 2214 82-83 -0.46551272177748615 2214 84-85 -0.3872468848945658 2214 86-87 -0.44541047572335657 2214 88-89 -0.3751216241018085 2214 90-91 -0.3603669118222257 2214 92-93 -0.31255131606597075 2214 94-95 -0.329752330222874 2214 96-97 -0.31295442375828486 2214 98-99 -0.3858880194477692 2214 100-101 -0.39525522256602486 2215 1 -0.20900058005031497 2215 2 -0.22959173754103546 2215 3 -0.18780195030027613 2215 4 -0.27210807014171934 2215 5 -0.2693436717037514 2215 6 -0.25432221752199524 2215 7 -0.3046554821537768 2215 8 -0.3235345709377313 2215 9 -0.405199833494855 2215 10-11 -0.383410463828497 2215 12-13 -0.3429204279198643 2215 14-15 -0.47303989930594526 2215 16-17 -0.4974064289502351 2215 18-19 -0.5218304873322239 2215 20-21 -0.5353104352302225 2215 22-23 -0.47196885025454094 2215 24-25 -0.4660517224230105 2215 26-27 -0.5345804077650413 2215 28-29 -0.5589935609674868 2215 30-31 -0.5564188867732156 2215 32-33 -0.5398904057949352 2215 34-35 -0.5460022777759406 2215 36-37 -0.5423924787625367 2215 38-39 -0.5963656915427649 2215 40-41 -0.48932664051828567 2215 42-43 -0.4552557922716076 2215 44-45 -0.44936548388429287 2215 46-47 -0.49863069104017654 2215 48-49 -0.45773326488070154 2215 50-51 -0.32158508464910796 2215 52-53 -0.5000520663750194 2215 54-55 -0.3702003627004373 2215 56-57 -0.3201514934939311 2215 58-59 -0.41134443059839043 2215 60-61 -0.35209666929644357 2215 62-63 -0.2935829931958125 2215 64-65 -0.3226949916856512 2215 66-67 -0.2799968154543393 2215 68-69 -0.2679820698567994 2215 70-71 -0.2533743577245886 2215 72-73 -0.26507997706645625 2215 74-75 -0.29600950357153266 2215 76-77 -0.2240584032737587 2215 78-79 -0.19872362689980605 2215 80-81 -0.23077358492221833 2215 82-83 -0.16266006008443412 2215 84-85 -0.148695203190087 2215 86-87 -0.17189640094699854 2215 88-89 -0.16701561236065743 2215 90-91 -0.21187544481053067 2215 92-93 -0.14488482881279907 2215 94-95 -0.20247704288746604 2215 96-97 -0.17381983430569647 2215 98-99 -0.2410547774936802 2215 100-101 -0.1828248202520939 2216 1 -0.2093570535046112 2216 2 -0.22857572948603533 2216 3 -0.20733991844815236 2216 4 -0.24687370821189347 2216 5 -0.21252230563284513 2216 6 -0.16002580561480784 2216 7 -0.2280481461896926 2216 8 -0.2542804722945107 2216 9 -0.31201892048567004 2216 10-11 -0.3455835231364972 2216 12-13 -0.40578065091577287 2216 14-15 -0.5542616891775509 2216 16-17 -0.6070484537957626 2216 18-19 -0.5627195246171368 2216 20-21 -0.47178753974066723 2216 22-23 -0.5618310206917272 2216 24-25 -0.5092373727287978 2216 26-27 -0.47571887427604054 2216 28-29 -0.5128018653326123 2216 30-31 -0.5552923952311133 2216 32-33 -0.5227473011816173 2216 34-35 -0.6095362099923705 2216 36-37 -0.6641293977224478 2216 38-39 -0.6190811309562605 2216 40-41 -0.5529202067268812 2216 42-43 -0.5644365809192422 2216 44-45 -0.5005296998726081 2216 46-47 -0.47319146838353987 2216 48-49 -0.5304159744277683 2216 50-51 -0.47467034955191423 2216 52-53 -0.4478515321894321 2216 54-55 -0.437634343176299 2216 56-57 -0.45075833209197214 2216 58-59 -0.3652141683349015 2216 60-61 -0.3923046936445047 2216 62-63 -0.3950124228532772 2216 64-65 -0.4736666111792829 2216 66-67 -0.4565708701163942 2216 68-69 -0.39176002787173303 2216 70-71 -0.3029943456649846 2216 72-73 -0.3080628906342753 2216 74-75 -0.3238058989797423 2216 76-77 -0.28980651770667265 2216 78-79 -0.2958246638704196 2216 80-81 -0.33377666187671196 2216 82-83 -0.2805974756072871 2216 84-85 -0.29430749390715505 2216 86-87 -0.2605489998184609 2216 88-89 -0.31673517850812516 2216 90-91 -0.28885528433330876 2216 92-93 -0.32080694714364455 2216 94-95 -0.3356035473979233 2216 96-97 -0.36031682816819455 2216 98-99 -0.3887664608533363 2216 100-101 -0.37651760374996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 273.0 11 2459.0 12 8789.0 13 16443.0 14 22445.0 15 26024.0 16 27629.0 17 28283.0 18 29928.0 19 32651.0 20 36342.0 21 55492.0 22 84882.0 23 104036.0 24 112415.0 25 113123.0 26 113425.0 27 120614.0 28 144994.0 29 176766.0 30 198200.0 31 228476.0 32 297791.0 33 401814.0 34 563998.0 35 898742.0 36 1299027.0 37 2668633.0 38 3798986.0 39 901320.0 40 504.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.104889461095873 29.518811286868008 27.341090836331723 23.035208415704396 2 22.838045251159695 29.639862789651815 26.790130303164933 20.731961656023554 3 22.137610204092724 28.298172009638733 26.593542470866616 22.97067531540193 4 22.940646168431073 27.595227874719484 26.553860527317575 22.91026542953187 5 23.414871763236718 28.337973814072438 26.518789232465235 21.728365190225617 6 23.282841003417794 28.108967607835645 25.537679947146145 23.070511441600424 7 23.55908837966303 28.516758309635502 25.873250470573833 22.050902840127634 8 22.887252139676605 28.518348464038702 26.067225335586503 22.52717406069819 9 22.2367031928063 28.26655270826468 26.432145794305296 23.064598304623722 10-11 22.52607134055929 28.5131584877328 26.101793214723262 22.858976956984645 12-13 22.48306125977945 28.728272817437166 26.111901482663164 22.67676444012022 14-15 22.294233786690285 28.94688960223897 26.24831513395664 22.51056147711411 16-17 22.17791941960483 28.93166874798434 26.356757293215203 22.533654539195627 18-19 22.023794303379727 28.756635898111217 26.582846884591866 22.63672291391719 20-21 21.96889002747851 28.85150578832184 26.657799891134353 22.521804293065298 22-23 22.05966772634377 28.60310724260426 27.045858149871542 22.29136688118043 24-25 22.037338583346624 28.370500062807103 27.31489324230725 22.277268111539023 26-27 22.070324298805836 28.36387974661089 27.37560278038897 22.190193174194302 28-29 22.056963805528753 28.43725458483865 27.38058899319094 22.12519261644165 30-31 22.07005261413393 28.352460999665347 27.40494872031725 22.172537665883468 32-33 22.107261428096095 28.354206973218606 27.404133666301544 22.13439793238376 34-35 22.10438476686419 28.387028878802106 27.41211640122009 22.09646995311362 36-37 22.128660456798478 28.368003809983467 27.316746427541194 22.18658930567686 38-39 22.174494672862345 28.411150458926294 27.19429638959979 22.22005847861158 40-41 22.20748878450018 28.502613100777836 27.07879722724747 22.211100887474522 42-43 22.226857024384294 28.518704842892795 27.0148953351795 22.23954279754341 44-45 22.21646220883182 28.557294040799352 27.019714068673554 22.206529681695265 46-47 22.20472879512085 28.627343507800703 26.99305368791112 22.174874009167322 48-49 22.23082690539129 28.570707122767878 26.981263259116243 22.21720271272459 50-51 22.298302358657807 28.54382200676135 27.00196965678557 22.155905977795275 52-53 22.25496003895294 28.571307559854464 26.993087738973742 22.180644662218853 54-55 22.200689852689504 28.557534391947055 26.994098438417975 22.24767731694547 56-57 22.204976444717534 28.596048590528593 26.990754282443564 22.208220682310312 58-59 22.247294899647674 28.631708893389973 26.97705335279661 22.14394285416574 60-61 22.21792099217964 28.62864445010543 26.948162884230047 22.205271673484887 62-63 22.227178247838427 28.61437301410491 26.963684868793887 22.19476386926278 64-65 22.20197949451948 28.686229614460274 26.953205032111534 22.158585858908715 66-67 22.20422444161999 28.657311762845456 26.944227762566097 22.194236032968462 68-69 22.24270822312791 28.702958198596377 26.924893891154174 22.12943968712154 70-71 22.231055083680896 28.735747039135788 26.901715249624335 22.13148262755898 72-73 22.221225599888513 28.69444880383479 26.88999281792661 22.19433277835009 74-75 22.198770213190826 28.717033447466772 26.953799111594677 22.130397227747725 76-77 22.232935269832556 28.786423729267273 26.88149630004311 22.099144700857057 78-79 22.213654337609448 28.75345096523946 26.90429814501791 22.128596552133185 80-81 22.19985902596225 28.800431759959615 26.907638352449638 22.092070861628493 82-83 22.2153115106139 28.859487267017194 26.869042507788432 22.056158714580473 84-85 22.170447136253486 28.84552572281921 26.863666367291177 22.12036077363613 86-87 22.164795432677728 28.92382397053097 26.872349380400372 22.039031216390924 88-89 22.177007090898528 28.96047007257905 26.846612963066104 22.01590987345632 90-91 22.165972144145208 28.987315604730025 26.7903142895202 22.056397961604567 92-93 22.18187792340791 29.01572447457766 26.81769489226261 21.984702709751822 94-95 22.151769769934223 29.106593723061263 26.77283038214527 21.968806124859245 96-97 22.126307322669017 29.14574428335497 26.756804981865844 21.97114341211017 98-99 22.106785979920264 29.209526255446878 26.733460076896353 21.950227687736508 100-101 22.13674720572315 29.305790527282415 26.647567766946974 21.909894500047464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 193214.0 1 102626.5 2 9124.0 3 5299.0 4 3848.5 5 2920.0 6 2263.0 7 1792.5 8 1457.5 9 1270.0 10 1194.0 11 1130.5 12 1119.0 13 1604.5 14 2348.0 15 3704.5 16 12191.5 17 11605.0 18 3763.5 19 4629.5 20 6712.0 21 10072.0 22 14315.0 23 19846.5 24 27020.0 25 36208.0 26 47882.5 27 61278.0 28 76795.5 29 94733.0 30 115688.0 31 138955.0 32 163625.5 33 191280.0 34 219877.5 35 251520.0 36 290011.0 37 329941.5 38 370445.0 39 409372.0 40 443703.5 41 473284.0 42 499676.5 43 523675.0 44 539035.0 45 546403.0 46 548862.0 47 544911.5 48 532846.5 49 513629.0 50 490489.5 51 467352.0 52 444773.0 53 418876.0 54 385676.5 55 344295.0 56 299214.5 57 257018.5 58 220604.0 59 185712.5 60 149855.5 61 118714.0 62 94176.5 63 73812.5 64 57594.5 65 44516.5 66 33622.0 67 25333.0 68 19420.5 69 15541.0 70 12914.5 71 10848.5 72 8975.0 73 7202.0 74 5459.0 75 3811.0 76 2651.5 77 1817.0 78 1105.0 79 551.0 80 208.5 81 84.5 82 34.5 83 15.5 84 7.5 85 4.5 86 3.5 87 2.5 88 1.5 89 0.5 90 0.5 91 1.0 92 2.5 93 2.0 94 0.5 95 1.5 96 2.5 97 2.5 98 1.5 99 4.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012785161030701327 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.196290257675332E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.4383306159538992E-4 38-39 0.0015102471467515943 40-41 0.008166521608360473 42-43 0.003911460202830187 44-45 5.553554322710889E-4 46-47 0.00480642147497928 48-49 3.9953628220941646E-5 50-51 0.0011586552184073076 52-53 0.007567217185046348 54-55 0.004251066042708191 56-57 9.189334490816579E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.598145128837666E-5 68-69 0.0 70-71 2.3972176932564984E-5 72-73 1.2785161030701327E-4 74-75 4.994203527617705E-4 76-77 0.0013823955364445808 78-79 5.953090604920306E-4 80-81 0.0011746366696956845 82-83 9.508963516584113E-4 84-85 9.868546170572585E-4 86-87 0.0017339874647888673 88-89 1.1986088466282494E-4 90-91 0.0012385624748491911 92-93 3.196290257675332E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2514508E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.62307213352055 #Duplication Level Percentage of deduplicated Percentage of total 1 81.85025827310682 52.894151445302704 2 10.057708673062198 12.999200661544673 3 3.307816465278436 6.4128378612040615 4 1.5501199514950148 4.00694053764287 5 0.8639240769489784 2.7914713971279497 6 0.534822714338884 2.0737132108420173 7 0.3685547928262263 1.6672000073374764 8 0.25029869653790143 1.2940056577035983 9 0.1822861052118338 1.0601899313438568 >10 0.9992021195860136 11.204668866347557 >50 0.03028564112358829 1.3007793932514669 >100 0.004549538207361269 0.3825513152739046 >500 3.712787655766339E-5 0.01948207495087767 >1k 1.1112988577631372E-4 0.1582303231183146 >5k 1.2347257222997887E-5 0.06825387955955202 >10k+ 1.2347257222997887E-5 1.6663234374490865 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 208834 1.6687351991784256 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.9953628220941646E-5 0.0 0.0 0.0 0.0 2 6.392580515350663E-5 0.0 0.0 0.0 0.0 3 1.0387943337444828E-4 0.0 0.0 0.0 0.0 4 1.358423359512016E-4 0.0 0.0 0.0 0.0 5 1.5981451288376658E-4 0.0 0.0 7.99072564418833E-6 7.99072564418833E-6 6 1.8378668981633157E-4 0.0 0.0 7.99072564418833E-6 7.99072564418833E-6 7 2.3972176932564987E-4 0.0 0.0 7.99072564418833E-6 7.99072564418833E-6 8 2.796753975465915E-4 0.0 0.0 7.99072564418833E-6 7.99072564418833E-6 9 3.276197514117215E-4 0.0 0.0 7.99072564418833E-6 7.99072564418833E-6 10-11 3.6757337963266314E-4 0.0 0.0 7.99072564418833E-6 7.99072564418833E-6 12-13 4.394899104303581E-4 0.0 0.0 1.1986088466282494E-5 2.7967539754659152E-5 14-15 5.473647066269005E-4 3.995362822094165E-6 0.0 1.598145128837666E-5 3.196290257675332E-5 16-17 6.951931310443846E-4 7.99072564418833E-6 0.0 1.598145128837666E-5 3.196290257675332E-5 18-19 8.470169182839629E-4 1.9976814110470823E-5 0.0 1.598145128837666E-5 3.196290257675332E-5 20-21 0.0010108267939898238 3.196290257675332E-5 0.0 1.598145128837666E-5 3.196290257675332E-5 22-23 0.0011466691299410253 4.394899104303581E-5 0.0 1.598145128837666E-5 3.196290257675332E-5 24-25 0.0013024882800026978 4.7944353865129975E-5 0.0 1.598145128837666E-5 3.196290257675332E-5 26-27 0.0014702935185306526 5.5935079509318304E-5 0.0 1.9976814110470823E-5 3.196290257675332E-5 28-29 0.0016540802083469842 5.5935079509318304E-5 0.0 3.9953628220941646E-5 3.196290257675332E-5 30-31 0.0018338715353412216 5.5935079509318304E-5 0.0 3.9953628220941646E-5 3.196290257675332E-5 32-33 0.0020256489508017417 5.993044233141247E-5 0.0 4.7944353865129975E-5 3.196290257675332E-5 34-35 0.0026768930908030904 6.79211679756008E-5 0.0 5.193971668722414E-5 3.196290257675332E-5 36-37 0.006396575878172757 7.191653079769496E-5 0.0 6.392580515350663E-5 3.595826539884748E-5 38-39 0.006540408939768148 7.191653079769496E-5 0.0 6.392580515350663E-5 3.9953628220941646E-5 40-41 0.006688237364185631 7.191653079769496E-5 0.0 7.591189361978913E-5 3.9953628220941646E-5 42-43 0.0068320704257810215 7.191653079769496E-5 0.0 7.990725644188329E-5 3.9953628220941646E-5 44-45 0.0069838942130206 7.591189361978913E-5 0.0 9.189334490816579E-5 3.9953628220941646E-5 46-47 0.007131722637438083 8.789798208607162E-5 0.0 1.0387943337444828E-4 4.7944353865129975E-5 48-49 0.007291537150321851 8.789798208607162E-5 0.0 1.0387943337444828E-4 4.7944353865129975E-5 50-51 0.007435370211917241 8.789798208607162E-5 0.0 1.1187015901863661E-4 4.7944353865129975E-5 52-53 0.007551235733757971 8.789798208607162E-5 0.0 1.1187015901863661E-4 5.193971668722414E-5 54-55 0.007758994600506868 8.789798208607162E-5 0.0 1.1187015901863661E-4 5.5935079509318304E-5 56-57 0.007938785927501104 8.789798208607162E-5 0.0 1.1986088466282494E-4 5.5935079509318304E-5 58-59 0.008138554068605813 1.0787479619654244E-4 0.0 1.238562474849191E-4 5.5935079509318304E-5 60-61 0.00837428047510937 1.1986088466282494E-4 0.0 1.2785161030701327E-4 5.5935079509318304E-5 62-63 0.008645965147011773 1.2785161030701327E-4 0.0 1.2785161030701327E-4 5.5935079509318304E-5 64-65 0.009005547801000248 1.2785161030701327E-4 0.0 1.3184697312910743E-4 5.5935079509318304E-5 66-67 0.009433051622964324 1.2785161030701327E-4 0.0 1.358423359512016E-4 5.5935079509318304E-5 68-69 0.009896513710327246 1.2785161030701327E-4 0.0 1.358423359512016E-4 5.5935079509318304E-5 70-71 0.010415910877199487 1.2785161030701327E-4 0.0 1.358423359512016E-4 5.5935079509318304E-5 72-73 0.011143066910820626 1.358423359512016E-4 0.0 1.478284244174841E-4 5.5935079509318304E-5 74-75 0.012237796324074426 1.358423359512016E-4 0.0 1.5182378723957825E-4 5.5935079509318304E-5 76-77 0.013668136214384136 1.358423359512016E-4 0.0 1.5182378723957825E-4 5.5935079509318304E-5 78-79 0.015741729519051006 1.358423359512016E-4 0.0 1.5182378723957825E-4 5.5935079509318304E-5 80-81 0.018250817371326146 1.3983769877329576E-4 0.0 1.5182378723957825E-4 5.5935079509318304E-5 82-83 0.02172678302654807 1.4383306159538992E-4 0.0 1.5981451288376658E-4 5.5935079509318304E-5 84-85 0.026577153492570383 1.4383306159538992E-4 0.0 1.678052385279549E-4 6.392580515350663E-5 86-87 0.03337726101577465 1.5182378723957825E-4 0.0 1.7180060135004908E-4 6.79211679756008E-5 88-89 0.04257458623223542 1.5182378723957825E-4 0.0 1.7579596417214324E-4 7.591189361978913E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3665 0.0 79.055115 1 TCGACGG 4755 0.0 61.832134 2 ATCTCGA 4545 0.0 37.726578 1 CGACGGC 7840 0.0 37.622673 3 TCTCGAG 5505 0.0 30.11218 2 ACGGCGC 10005 0.0 29.528908 5 GACGGCG 10000 0.0 29.496174 4 GGGCGCG 5480 0.0 29.469479 8 TCGAGGG 5765 0.0 28.177399 4 CGGCGCG 11045 0.0 26.87747 6 CTCGAGG 6210 0.0 26.158245 3 AGGGCGC 6515 0.0 25.006552 7 TATCTCG 2270 0.0 24.899761 2 CGAGGGC 6735 0.0 23.978136 5 CGCGCCG 15935 0.0 22.952047 9 GGCGCGC 16425 0.0 22.440392 7 GCGCGCC 16645 0.0 22.257938 8 GAGGGCG 7925 0.0 20.73724 6 CGCCGGA 15890 0.0 16.35107 9 GCGCCGG 17150 0.0 15.232553 8 >>END_MODULE