FastQCFastQC Report
Tue 31 May 2016
SRR1567937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12514508
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT4247533.394084689545925No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT348060.278125196771619No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA293200.23428807588760184No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT235910.18850920867204687No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227940.18214060033362878No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126800.10132240116830801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1019800.093.9783551
TCGACGG1045150.091.6257552
TATCTCG752600.089.9488752
ATATCTC767100.088.823231
CGACGGC1081050.088.4160463
GACGGCG1113550.086.4497764
ACGGCGC1122700.086.384065
CGGCGCG1129550.085.725646
ATCTCGA824850.082.012523
TCTCGAG824950.079.014284
CTCGAGG864400.074.869665
TCGAGGG867800.074.642016
CGAGGGC886150.073.219647
AGGGCGC915150.072.098369
GAGGGCG917050.071.5242548
TACGGCG13750.055.9624184
CGCGCCG2042800.049.2033279
GGCGCGC2063100.049.1658367
GCGCGCC2065450.049.0823068
CTCGACG18800.045.7303855
Produced by FastQC (version 0.11.5)