FastQCFastQC Report
Tue 31 May 2016
SRR1567936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7098967
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107960.1520784643737603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG43850.086.874511
TCGACGG49500.076.862582
CGACGGC70300.054.1208763
ACGGCGC76950.049.443765
GACGGCG79200.048.0990834
CGGCGCG82650.045.9770286
TATCTCG21350.043.1612
CGCGCCG110200.037.500019
GCGCGCC117800.035.16138
GGCGCGC118350.035.078177
ATATCTC30050.031.613541
ATCTCGA32000.028.9449123
TCTCGAG39000.023.5060844
TCGAGGG40750.022.030686
CCATATT104900.020.39883618-19
AGGGCGC44550.020.2581429
ATCCATA107650.019.76728416-17
CGCCGGA115450.019.66652910-11
CTCGAGG47200.019.4224015
GCCGGAT113150.019.18464912-13
Produced by FastQC (version 0.11.5)