##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567936_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7098967 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09997229174329 33.0 31.0 34.0 30.0 34.0 2 32.26568865582838 34.0 31.0 34.0 30.0 34.0 3 32.29812844601194 34.0 31.0 34.0 30.0 34.0 4 35.7491933685563 37.0 35.0 37.0 33.0 37.0 5 35.66271149027739 37.0 35.0 37.0 33.0 37.0 6 35.64917177386513 37.0 35.0 37.0 33.0 37.0 7 35.56413996571614 37.0 35.0 37.0 33.0 37.0 8 35.50023503419582 37.0 35.0 37.0 33.0 37.0 9 37.04897008254863 39.0 37.0 39.0 33.0 39.0 10-11 37.056052944041014 39.0 37.0 39.0 33.0 39.0 12-13 36.98009703383605 39.0 37.0 39.0 33.0 39.0 14-15 38.300205931370016 40.0 38.0 41.0 33.0 41.0 16-17 38.31362316517318 40.0 38.0 41.0 33.0 41.0 18-19 38.40672318944432 40.5 38.0 41.0 33.0 41.0 20-21 38.46572029423436 41.0 38.0 41.0 33.0 41.0 22-23 38.48949375310521 40.0 38.0 41.0 33.5 41.0 24-25 38.52203855856774 40.0 38.0 41.0 34.0 41.0 26-27 38.51647408700449 40.0 38.0 41.0 34.0 41.0 28-29 38.4457441484092 40.0 38.0 41.0 34.0 41.0 30-31 38.39454810819659 40.0 38.0 41.0 33.5 41.0 32-33 38.36437047812731 40.0 38.0 41.0 33.0 41.0 34-35 38.26593869220691 40.0 38.0 41.0 33.0 41.0 36-37 38.20648250653933 40.0 38.0 41.0 33.0 41.0 38-39 38.173158855365855 40.0 38.0 41.0 33.0 41.0 40-41 38.05538643861847 40.0 38.0 41.0 33.0 41.0 42-43 37.91941524731697 40.0 37.0 41.0 32.5 41.0 44-45 37.82125272592477 40.0 37.0 41.0 32.0 41.0 46-47 37.72981491532501 40.0 37.0 41.0 32.0 41.0 48-49 37.74906475829512 40.0 37.0 41.0 32.5 41.0 50-51 37.96751795014683 40.0 37.0 41.0 33.0 41.0 52-53 37.88540065054536 40.0 36.5 41.0 33.0 41.0 54-55 37.68497099648442 40.0 36.0 41.0 32.5 41.0 56-57 37.43860170078266 39.0 35.5 41.0 32.0 41.0 58-59 37.16374741564512 39.0 35.0 41.0 32.0 41.0 60-61 36.86818258205736 38.5 35.0 40.5 32.0 41.0 62-63 36.52013673256968 37.5 35.0 40.0 31.0 41.0 64-65 36.188095112993196 37.0 35.0 40.0 31.0 41.0 66-67 35.82666985210665 36.5 35.0 39.0 31.0 41.0 68-69 35.43946464323612 36.0 34.5 39.0 30.5 41.0 70-71 35.07186094821965 35.5 34.0 38.5 30.0 40.0 72-73 34.71503318722287 35.0 34.0 37.0 30.0 39.5 74-75 34.366331326797265 35.0 34.0 37.0 30.0 39.0 76-77 34.077525997796585 35.0 34.0 36.5 30.0 39.0 78-79 33.783358550617294 35.0 34.0 36.0 30.0 37.5 80-81 33.564982482662614 35.0 34.0 35.5 30.0 37.0 82-83 33.36338899730059 35.0 34.0 35.0 29.0 37.0 84-85 33.214028745309 35.0 34.0 35.0 29.0 36.0 86-87 33.08599490601942 35.0 34.0 35.0 29.0 36.0 88-89 32.959883811264376 35.0 33.0 35.0 29.0 36.0 90-91 32.84611239917019 35.0 33.0 35.0 29.0 35.5 92-93 32.75077423236367 35.0 33.0 35.0 29.0 35.0 94-95 32.65801848353429 35.0 33.0 35.0 29.0 35.0 96-97 32.55867262096021 35.0 33.0 35.0 29.0 35.0 98-99 32.44682282647602 35.0 33.0 35.0 29.0 35.0 100-101 31.991958618768052 34.5 32.5 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3277639178940177 1101 2 0.35698082373563267 1101 3 0.3665891703867956 1101 4 0.3261543802537048 1101 5 0.35789376084846936 1101 6 0.36422825312313734 1101 7 0.376045524835277 1101 8 0.4091854563772799 1101 9 0.5278621138667106 1101 10-11 0.5283945899119473 1101 12-13 0.5156709979296963 1101 14-15 0.6366487397226663 1101 16-17 0.6753703943634122 1101 18-19 0.6614692782004212 1101 20-21 0.5735050148071892 1101 22-23 0.5280331868000374 1101 24-25 0.5674335884768738 1101 26-27 0.5163051607306528 1101 28-29 0.4976094476085251 1101 30-31 0.48004237225313773 1101 32-33 0.44510771068735977 1101 34-35 0.4115134987218667 1101 36-37 0.4702786438439901 1101 38-39 0.5171945762592927 1101 40-41 0.4718235635513608 1101 42-43 0.4683195463836256 1101 44-45 0.41508251610579805 1101 46-47 0.44565029086321317 1101 48-49 0.42818008269873076 1101 50-51 0.4232642368653714 1101 52-53 0.3851358614180711 1101 54-55 0.3723987682519052 1101 56-57 0.39075430699066516 1101 58-59 0.36050090568163995 1101 60-61 0.3141101427316215 1101 62-63 0.346617568977706 1101 64-65 0.30355079150729125 1101 66-67 0.33246550664595276 1101 68-69 0.35102009027723824 1101 70-71 0.3135934886816898 1101 72-73 0.3382599390631782 1101 74-75 0.3582479317395908 1101 76-77 0.3091094488685826 1101 78-79 0.35559049350709415 1101 80-81 0.3515622208570406 1101 82-83 0.35811716121861537 1101 84-85 0.31698999380825654 1101 86-87 0.26873495991891616 1101 88-89 0.3149985782795426 1101 90-91 0.305742447967738 1101 92-93 0.3006566396745569 1101 94-95 0.29933796891567965 1101 96-97 0.35161488624880377 1101 98-99 0.33298991064693695 1101 100-101 0.3389624634922086 1102 1 0.3121444110609062 1102 2 0.31986941382422174 1102 3 0.37573358770237064 1102 4 0.3235289826806351 1102 5 0.31735709286083846 1102 6 0.3608991761494451 1102 7 0.32776308481700767 1102 8 0.3782548306742797 1102 9 0.46723970286394945 1102 10-11 0.5166365659722416 1102 12-13 0.528404235592312 1102 14-15 0.6148851663307795 1102 16-17 0.5592447531140863 1102 18-19 0.6056085469863106 1102 20-21 0.6374365473202914 1102 22-23 0.6222306548234471 1102 24-25 0.6021993701659198 1102 26-27 0.5567060540020705 1102 28-29 0.5321412134625447 1102 30-31 0.5909571443931725 1102 32-33 0.5622550696457509 1102 34-35 0.5403549153442029 1102 36-37 0.4920071617020625 1102 38-39 0.47360098009944096 1102 40-41 0.48088782659036156 1102 42-43 0.5029405930411315 1102 44-45 0.48446790898867675 1102 46-47 0.4222624626280691 1102 48-49 0.43513851549878524 1102 50-51 0.44376350926157215 1102 52-53 0.4403002517247714 1102 54-55 0.4634558547784309 1102 56-57 0.44284251880184655 1102 58-59 0.4283130203100214 1102 60-61 0.4803782373807479 1102 62-63 0.4783589949280298 1102 64-65 0.4289340451967334 1102 66-67 0.43038365620534336 1102 68-69 0.5019044265581201 1102 70-71 0.4777882801949431 1102 72-73 0.46623721663027595 1102 74-75 0.4694797197350056 1102 76-77 0.46247511757404425 1102 78-79 0.45437074728901905 1102 80-81 0.45524325841220303 1102 82-83 0.4354662547006498 1102 84-85 0.4214763567019375 1102 86-87 0.40607158148817746 1102 88-89 0.4091616052483289 1102 90-91 0.4329252643897661 1102 92-93 0.45868140540184754 1102 94-95 0.4972565314856823 1102 96-97 0.47072695173898893 1102 98-99 0.48859579921104057 1102 100-101 0.5001694809250807 1103 1 0.26510284663530115 1103 2 0.3145354041590096 1103 3 0.3365895639941101 1103 4 0.2405117761978417 1103 5 0.29288850481191986 1103 6 0.263390992700252 1103 7 0.2794567464544926 1103 8 0.3117755379241913 1103 9 0.44543814463526843 1103 10-11 0.4335728075326699 1103 12-13 0.3953039202484021 1103 14-15 0.5563883722438518 1103 16-17 0.5356489580326524 1103 18-19 0.5113197199556865 1103 20-21 0.46120353266254455 1103 22-23 0.4979570952354422 1103 24-25 0.48448663796835234 1103 26-27 0.44927930524270465 1103 28-29 0.42244556280961376 1103 30-31 0.3958452747686394 1103 32-33 0.4685118975159597 1103 34-35 0.42513644848529 1103 36-37 0.4002139493421808 1103 38-39 0.42299220644240165 1103 40-41 0.4033129761413292 1103 42-43 0.4567935358344499 1103 44-45 0.40626929230393927 1103 46-47 0.33993119160722074 1103 48-49 0.35509528689694747 1103 50-51 0.38836195802942797 1103 52-53 0.357351286462567 1103 54-55 0.36686254346753344 1103 56-57 0.3674566348144239 1103 58-59 0.3671741090710796 1103 60-61 0.3569144519545375 1103 62-63 0.31497182157883685 1103 64-65 0.2645387458698991 1103 66-67 0.28539954833873793 1103 68-69 0.34536121544761755 1103 70-71 0.32514428897059844 1103 72-73 0.29914902528499 1103 74-75 0.32958254383706986 1103 76-77 0.2863284351818294 1103 78-79 0.3208364717554062 1103 80-81 0.316310854951908 1103 82-83 0.2868729333946902 1103 84-85 0.2855923193004486 1103 86-87 0.2650995626375092 1103 88-89 0.2797133571311434 1103 90-91 0.31998771847499086 1103 92-93 0.3080995434235305 1103 94-95 0.3226334449135351 1103 96-97 0.362099572291811 1103 98-99 0.3402772918721766 1103 100-101 0.3558973706196866 1104 1 0.21969050204909024 1104 2 0.2216842253015372 1104 3 0.23444129360363064 1104 4 0.2172022823240951 1104 5 0.2044963534751858 1104 6 0.1955160421935105 1104 7 0.190374140593498 1104 8 0.20866391784982596 1104 9 0.3066450954238391 1104 10-11 0.27613291641287674 1104 12-13 0.34262736948444683 1104 14-15 0.4300275681260217 1104 16-17 0.43417744779115 1104 18-19 0.426331509026582 1104 20-21 0.4574745939667082 1104 22-23 0.4397348434633628 1104 24-25 0.46503142105639483 1104 26-27 0.4187807605181675 1104 28-29 0.4016579030294949 1104 30-31 0.34502654463416604 1104 32-33 0.33371940699533553 1104 34-35 0.3600023279569271 1104 36-37 0.327645529563938 1104 38-39 0.361198997463255 1104 40-41 0.36891005090369333 1104 42-43 0.34923995659114837 1104 44-45 0.30508014420323804 1104 46-47 0.29403986143682914 1104 48-49 0.3194010118404478 1104 50-51 0.31141761971088755 1104 52-53 0.31289263107159826 1104 54-55 0.23085968352626196 1104 56-57 0.23858569636269777 1104 58-59 0.24877977953479302 1104 60-61 0.21171935176231926 1104 62-63 0.24628276527014492 1104 64-65 0.22758102080334197 1104 66-67 0.22383320951012564 1104 68-69 0.2426870463141455 1104 70-71 0.2391283499085901 1104 72-73 0.21959032156676983 1104 74-75 0.23247706340762164 1104 76-77 0.2530747210787112 1104 78-79 0.274805508574417 1104 80-81 0.316049490853203 1104 82-83 0.29517209694621727 1104 84-85 0.2502785891014767 1104 86-87 0.2655531590483946 1104 88-89 0.26658799802592625 1104 90-91 0.3069584680832662 1104 92-93 0.3429101095271676 1104 94-95 0.29985757519666123 1104 96-97 0.30247355278030597 1104 98-99 0.3273115913205302 1104 100-101 0.30885186758460037 1105 1 0.13754531078436827 1105 2 0.17184347553951085 1105 3 0.20397667787802476 1105 4 0.14845082008831412 1105 5 0.1897097888521131 1105 6 0.16467570688423905 1105 7 0.17453402583731759 1105 8 0.18734535798821383 1105 9 0.25086449259224963 1105 10-11 0.295837093937692 1105 12-13 0.28844751054801776 1105 14-15 0.34017601438019085 1105 16-17 0.3672035810716068 1105 18-19 0.2656578269014034 1105 20-21 0.34090796332110784 1105 22-23 0.3623622105318063 1105 24-25 0.3536241154653652 1105 26-27 0.3775015619784625 1105 28-29 0.33006491044522335 1105 30-31 0.35700322009333973 1105 32-33 0.3929828197012881 1105 34-35 0.355450205807621 1105 36-37 0.33578865339677577 1105 38-39 0.3379209832078871 1105 40-41 0.3043861353897057 1105 42-43 0.3169506342751873 1105 44-45 0.2690735087506795 1105 46-47 0.21964229868090968 1105 48-49 0.2214475613225133 1105 50-51 0.14763166468718936 1105 52-53 0.21465242257645656 1105 54-55 0.16792505561645754 1105 56-57 0.22270392590905175 1105 58-59 0.1618685763888763 1105 60-61 0.18809449245259202 1105 62-63 0.22272752046323774 1105 64-65 0.20357806492440034 1105 66-67 0.18172078012023007 1105 68-69 0.19999991247708238 1105 70-71 0.14882585083171307 1105 72-73 0.1954496563942456 1105 74-75 0.21165482592073914 1105 76-77 0.1870269435134304 1105 78-79 0.18470756663864307 1105 80-81 0.16202507508118202 1105 82-83 0.20176114373449394 1105 84-85 0.17599153668979994 1105 86-87 0.18271513494840974 1105 88-89 0.20485026027087372 1105 90-91 0.22667739850523105 1105 92-93 0.2256085617121215 1105 94-95 0.2373349256417967 1105 96-97 0.2691099888669015 1105 98-99 0.23117384602146984 1105 100-101 0.24039215109896617 1106 1 0.13763053423227944 1106 2 0.16426560640412902 1106 3 0.1913638745662638 1106 4 0.06847808243808373 1106 5 0.13411290243519858 1106 6 0.09100700622049374 1106 7 0.10702608989239337 1106 8 0.07682256009612587 1106 9 0.17149680518934218 1106 10-11 0.18631716377934993 1106 12-13 0.1706342652719215 1106 14-15 0.290818727708654 1106 16-17 0.28027390977209876 1106 18-19 0.26812354777658953 1106 20-21 0.2332800356601581 1106 22-23 0.24841413180662641 1106 24-25 0.2741990657596318 1106 26-27 0.23193289522102134 1106 28-29 0.22722358853400948 1106 30-31 0.2836132452999607 1106 32-33 0.2215164843934545 1106 34-35 0.24251571210812273 1106 36-37 0.20431200507917424 1106 38-39 0.21660680674786903 1106 40-41 0.22903348499578158 1106 42-43 0.23410789356232442 1106 44-45 0.1697639636100874 1106 46-47 0.23297849059441944 1106 48-49 0.21488388745008535 1106 50-51 0.19057480468591592 1106 52-53 0.20587770653701654 1106 54-55 0.17810282154546542 1106 56-57 0.19965175558115078 1106 58-59 0.17525427380044079 1106 60-61 0.22141991177140596 1106 62-63 0.24229416809812676 1106 64-65 0.21673403661091584 1106 66-67 0.16703831898757926 1106 68-69 0.20477091903675415 1106 70-71 0.23103020708670385 1106 72-73 0.26116387618111503 1106 74-75 0.2580741920291487 1106 76-77 0.2707788233302537 1106 78-79 0.2770094521573725 1106 80-81 0.2690402291794314 1106 82-83 0.2844807881492386 1106 84-85 0.2115577853820767 1106 86-87 0.22111344710841507 1106 88-89 0.17416556643262737 1106 90-91 0.23394726945149102 1106 92-93 0.21165853785565503 1106 94-95 0.2520862005609317 1106 96-97 0.29553069749729843 1106 98-99 0.3071093111424048 1106 100-101 0.27280262452484294 1107 1 0.09454933497718088 1107 2 0.06457650862218856 1107 3 0.12012274624760266 1107 4 0.12296149790118704 1107 5 0.1149263759136332 1107 6 0.027892097353067413 1107 7 0.08524858814305247 1107 8 0.06404770920700997 1107 9 0.13836827178887035 1107 10-11 0.11553674275165804 1107 12-13 0.19028941279081124 1107 14-15 0.17599121873978163 1107 16-17 0.118363180082099 1107 18-19 0.1699197740030769 1107 20-21 0.20998106601547306 1107 22-23 0.18814232607469705 1107 24-25 0.17982848771660542 1107 26-27 0.16479180725762888 1107 28-29 0.18467098784019242 1107 30-31 0.2222349090479554 1107 32-33 0.2137471221919114 1107 34-35 0.24455818881128266 1107 36-37 0.2422710926056979 1107 38-39 0.28598353989584524 1107 40-41 0.19849671356583087 1107 42-43 0.21401559475558685 1107 44-45 0.1571111041241977 1107 46-47 0.12930125504179557 1107 48-49 0.2451955116203024 1107 50-51 0.14729900230088333 1107 52-53 0.15553214135955074 1107 54-55 0.13268823589046974 1107 56-57 0.09380051845133863 1107 58-59 0.13035569882293174 1107 60-61 0.0640100447216696 1107 62-63 0.05782886622957051 1107 64-65 0.14948970023304753 1107 66-67 0.061363867578656084 1107 68-69 0.07950439836485401 1107 70-71 0.08448294328538708 1107 72-73 0.07230096008676412 1107 74-75 0.0800661968717904 1107 76-77 0.0977939491535551 1107 78-79 0.08924020102538321 1107 80-81 0.09387746599085034 1107 82-83 0.13458445307457367 1107 84-85 0.0854164993446318 1107 86-87 0.0481881339848087 1107 88-89 0.07298059303379034 1107 90-91 0.03721359390049628 1107 92-93 0.013814675744818317 1107 94-95 0.007317623250962413 1107 96-97 -0.03819640828905335 1107 98-99 0.0010276972276059837 1107 100-101 0.03872129939091451 1108 1 0.11188309796468587 1108 2 0.1071690384682853 1108 3 0.17019579681910102 1108 4 0.10604481312257263 1108 5 0.10777036931678907 1108 6 0.03393744032207735 1108 7 0.07532546416985753 1108 8 0.09325628061451852 1108 9 0.05165404822287201 1108 10-11 0.11258810058639313 1108 12-13 0.10527758706022894 1108 14-15 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54-55 -0.39174835625131266 2214 56-57 -0.3619328844555625 2214 58-59 -0.35212892481145275 2214 60-61 -0.3578254848020208 2214 62-63 -0.30710030459195536 2214 64-65 -0.3181619705680845 2214 66-67 -0.3341826029014143 2214 68-69 -0.3472173356988577 2214 70-71 -0.4036651200863943 2214 72-73 -0.3106372122075953 2214 74-75 -0.2721417886721369 2214 76-77 -0.2951280984304958 2214 78-79 -0.2997750008833435 2214 80-81 -0.2716216861989338 2214 82-83 -0.2755587082472033 2214 84-85 -0.26247446291055354 2214 86-87 -0.2606330772103007 2214 88-89 -0.2653795797789229 2214 90-91 -0.27516232521426787 2214 92-93 -0.22761778151794942 2214 94-95 -0.18956691735952802 2214 96-97 -0.2570361665093941 2214 98-99 -0.29019665117909454 2214 100-101 -0.25676980613227585 2215 1 -0.3519425733934689 2215 2 -0.340940691378659 2215 3 -0.4329403531943896 2215 4 -0.3337013249291232 2215 5 -0.3433307356353055 2215 6 -0.3716883478333415 2215 7 -0.3944713658694283 2215 8 -0.37739953658562087 2215 9 -0.553961716367958 2215 10-11 -0.5207672866367545 2215 12-13 -0.5515976308157278 2215 14-15 -0.6786334222838448 2215 16-17 -0.6580693754201619 2215 18-19 -0.6397058404009712 2215 20-21 -0.6115389597245269 2215 22-23 -0.6101671613204402 2215 24-25 -0.6639272919648675 2215 26-27 -0.5502358689663822 2215 28-29 -0.5901848118358615 2215 30-31 -0.5734220376662051 2215 32-33 -0.5763011470880244 2215 34-35 -0.5287490326325397 2215 36-37 -0.6054018243225698 2215 38-39 -0.5785760555272077 2215 40-41 -0.5535635819684259 2215 42-43 -0.46527546209916437 2215 44-45 -0.5042294627882171 2215 46-47 -0.47160784312163173 2215 48-49 -0.4566253797582718 2215 50-51 -0.49775820130139437 2215 52-53 -0.42284091538017066 2215 54-55 -0.4082582196045905 2215 56-57 -0.41885245584690267 2215 58-59 -0.47765628346741096 2215 60-61 -0.4761181877971552 2215 62-63 -0.4355294535456977 2215 64-65 -0.4338237702678853 2215 66-67 -0.5026701325315486 2215 68-69 -0.4467453716523764 2215 70-71 -0.41735205194633096 2215 72-73 -0.4179228037351592 2215 74-75 -0.3696550120712132 2215 76-77 -0.4441345101711818 2215 78-79 -0.4273525512126355 2215 80-81 -0.4368637281910921 2215 82-83 -0.471046910221105 2215 84-85 -0.4418431547371924 2215 86-87 -0.43507862103137995 2215 88-89 -0.4380042835102529 2215 90-91 -0.3936931502330978 2215 92-93 -0.4041867717539134 2215 94-95 -0.4072070682408935 2215 96-97 -0.4199317792019457 2215 98-99 -0.3640125734700135 2215 100-101 -0.30676093570130547 2216 1 -0.5608260982351467 2216 2 -0.5385837135886327 2216 3 -0.541081292965945 2216 4 -0.5066020674038612 2216 5 -0.5115605886784138 2216 6 -0.5282594487480097 2216 7 -0.4825652049071252 2216 8 -0.46175317837821694 2216 9 -0.6430563063653665 2216 10-11 -0.6748302524418364 2216 12-13 -0.7587580442714383 2216 14-15 -0.9721949727603487 2216 16-17 -0.9347754815214842 2216 18-19 -0.9227051257807872 2216 20-21 -0.8440516503782618 2216 22-23 -0.9028099575288167 2216 24-25 -0.8987328817999227 2216 26-27 -0.9210538265193833 2216 28-29 -0.9590827814726239 2216 30-31 -0.9167117414848036 2216 32-33 -0.952966383273143 2216 34-35 -0.9104926075931346 2216 36-37 -0.8223382465720377 2216 38-39 -0.9525151107384531 2216 40-41 -0.9206388081622379 2216 42-43 -0.9124214617897621 2216 44-45 -0.8537706709138391 2216 46-47 -0.9552775033872578 2216 48-49 -0.8244579957649236 2216 50-51 -0.7486523557001021 2216 52-53 -0.6590087639266216 2216 54-55 -0.707169905521738 2216 56-57 -0.6775951494529622 2216 58-59 -0.6729948995494368 2216 60-61 -0.649376119686309 2216 62-63 -0.6375204638880234 2216 64-65 -0.6532739130212519 2216 66-67 -0.6238142840082688 2216 68-69 -0.6099787360811888 2216 70-71 -0.5351156004149189 2216 72-73 -0.5705237236220242 2216 74-75 -0.5550786481980765 2216 76-77 -0.5956999686308677 2216 78-79 -0.5780388186139405 2216 80-81 -0.5680189749501849 2216 82-83 -0.5495752184648168 2216 84-85 -0.5793385222214908 2216 86-87 -0.5489527612038785 2216 88-89 -0.5699616463339225 2216 90-91 -0.7036404806542293 2216 92-93 -0.6585660484377698 2216 94-95 -0.696242518566244 2216 96-97 -0.7560599990309314 2216 98-99 -0.7758414267137468 2216 100-101 -0.7649366928213297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 56.0 11 863.0 12 3205.0 13 6455.0 14 9123.0 15 10714.0 16 11293.0 17 11864.0 18 13331.0 19 16912.0 20 17264.0 21 21588.0 22 28439.0 23 34880.0 24 41057.0 25 46518.0 26 51992.0 27 60365.0 28 75933.0 29 93206.0 30 104077.0 31 124639.0 32 169381.0 33 230069.0 34 319092.0 35 471621.0 36 854588.0 37 1877577.0 38 2054407.0 39 338290.0 40 166.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.444461223223723 26.44366448163761 25.11737710866761 25.994497186471055 2 25.34457478109139 26.66209041399967 25.877736859461383 22.115597945447558 3 24.066431073704102 25.39601888556462 25.295919814812496 25.241630225918787 4 25.33865842734584 24.35734663930682 25.14310884949881 25.16088608384854 5 25.720073278287753 25.364528491125114 25.170711505127862 23.744686725459275 6 25.56452227486055 24.71632844609645 24.15638782374957 25.562761455293426 7 26.346018533713767 25.198314399795464 24.307281846412607 24.14838522007817 8 25.451235313457993 25.175303140820354 24.674432999391733 24.699028546329924 9 24.425221254582898 24.82559037964634 24.97323195306798 25.775956412702783 10-11 25.14751333457155 25.136187691265498 24.563277770769684 25.153021203393273 12-13 25.022755489579176 25.29163053691968 24.560919243597173 25.12469472990398 14-15 24.8954111195076 25.514848039472216 24.690864379545662 24.89887646147452 16-17 24.77726127776667 25.693656525434406 24.80527243687153 24.72380975992739 18-19 24.424017143531294 25.35433192877053 25.134677792823172 25.086973134875006 20-21 24.41157283869611 25.637878018027134 25.034783229729058 24.915765913547702 22-23 24.432308249918616 25.455619106272785 25.531179395537407 24.58089324827119 24-25 24.42265868249302 25.04035497445582 25.889186416386707 24.647799926664458 26-27 24.401877108646396 25.11424134722911 26.012486588584576 24.47139495553992 28-29 24.457068206348033 25.16830639088159 25.97153694950724 24.40308845326314 30-31 24.44434427257142 25.07960377015626 25.989334982412792 24.486716974859522 32-33 24.452803780182776 25.116602309423016 25.992433210929665 24.438160699464536 34-35 24.484348361690596 25.217452244956316 25.9268269577182 24.37137243563489 36-37 24.53111675701466 25.14290709125001 25.807044629160703 24.518931522574626 38-39 24.584495026787508 25.19996247269973 25.734627893715473 24.48091460679729 40-41 24.721336216945367 25.33912328371156 25.358020399305985 24.58152010003709 42-43 24.749662468425857 25.314068648453077 25.32759174558603 24.60867713753504 44-45 24.673302796963473 25.43589971864214 25.327700870260482 24.563096614133904 46-47 24.67942478318649 25.537656371540724 25.219532218672093 24.563386626600693 48-49 24.710205796179896 25.458129740071616 25.185412084688412 24.646252379060073 50-51 24.65248534670112 25.484262341153947 25.276441355284433 24.586810956860504 52-53 24.676728479818 25.50320101639068 25.27526543175612 24.544805072035196 54-55 24.654680643519804 25.400643147880057 25.3011744366046 24.64350177199554 56-57 24.616668236156553 25.455867552614947 25.394294715265776 24.533169495962724 58-59 24.710750129232075 25.47449953079196 25.365929797911956 24.44882054206401 60-61 24.653203015763143 25.372357044673834 25.340070652542916 24.63436928702011 62-63 24.6911064666169 25.422635434141334 25.342335018602004 24.54392308063976 64-65 24.701785816287483 25.5169467273863 25.257676592782907 24.52359086354331 66-67 24.710778342820863 25.44806871196894 25.226029364554027 24.61512358065617 68-69 24.673963127311342 25.54453345113451 25.219598851494872 24.56190457005928 70-71 24.714715535372964 25.569163795239504 25.188439388434965 24.527681280952567 72-73 24.723899970235106 25.49655463956939 25.131233882338094 24.64831150785741 74-75 24.718687944316407 25.54701268508503 25.210336940571775 24.523962430026792 76-77 24.688683578892533 25.5864339135539 25.196588461391634 24.52829404616193 78-79 24.751735005952273 25.475452977876923 25.20516013104442 24.567651885126384 80-81 24.675702817043664 25.526453355819235 25.263591167560012 24.534252659577092 82-83 24.743858733743313 25.575663264080735 25.208799848034154 24.4716781541418 84-85 24.68657398459832 25.5212591583786 25.202283037355873 24.589883819667207 86-87 24.690603334415204 25.55422622589457 25.236884573427453 24.51828586626277 88-89 24.709233529384587 25.602966068746035 25.181241287289275 24.506559114580103 90-91 24.70921479489711 25.555458747869984 25.172529960706957 24.562796496525944 92-93 24.71041361494289 25.581379132509724 25.199313939272876 24.508893313274513 94-95 24.72066869908907 25.64191472559621 25.19104319841418 24.446373376900535 96-97 24.663560110523154 25.597576548130608 25.148440652968425 24.590422688377814 98-99 24.66991761806944 25.640568549070125 25.18824797619647 24.501265856663963 100-101 24.73246626321346 25.672349819470146 25.134961584890302 24.46022233242609 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9485.0 1 5350.5 2 992.0 3 660.0 4 482.0 5 354.0 6 249.0 7 200.0 8 179.5 9 167.0 10 176.5 11 161.0 12 166.5 13 214.0 14 265.5 15 439.0 16 2831.0 17 3052.5 18 946.5 19 1102.5 20 1593.0 21 2260.0 22 3237.0 23 4667.5 24 6442.0 25 8500.0 26 11192.0 27 14897.0 28 19660.0 29 25059.0 30 31474.5 31 38489.0 32 46102.0 33 54743.5 34 64102.0 35 74621.0 36 88097.0 37 105128.5 38 123516.5 39 141202.5 40 156763.0 41 170312.0 42 185438.5 43 202030.0 44 219700.5 45 242250.5 46 268651.5 47 292660.5 48 310433.5 49 321418.0 50 322640.0 51 320374.0 52 323520.0 53 327679.0 54 321804.0 55 307631.5 56 288898.0 57 266269.0 58 243795.0 59 220255.0 60 192592.0 61 164096.0 62 135652.0 63 104660.0 64 77536.0 65 56319.0 66 40641.0 67 30843.0 68 23475.5 69 18195.0 70 14380.5 71 10710.5 72 8184.5 73 6142.5 74 4585.5 75 3674.0 76 2920.5 77 1997.5 78 1078.0 79 504.5 80 239.5 81 126.0 82 56.0 83 17.5 84 3.5 85 4.0 86 4.5 87 3.5 88 1.0 89 1.0 90 1.5 91 1.0 92 0.5 93 1.0 94 2.5 95 1.5 96 0.5 97 2.0 98 4.5 99 6.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.465874964625135E-4 2 0.0 3 0.0 4 0.0 5 2.8173113074057112E-5 6 0.0 7 1.6903867844434266E-4 8 0.0017044733409804552 9 2.53558017666514E-4 10-11 4.578130874534281E-4 12-13 0.0018382956280822266 14-15 0.0014156989319713698 16-17 1.4790884363879983E-4 18-19 0.0011198812446937701 20-21 0.0 22-23 0.0 24-25 0.0012677900883325702 26-27 9.931022358605132E-4 28-29 1.26779008833257E-4 30-31 8.029337226106277E-4 32-33 7.184143833884564E-4 34-35 0.001542477940804627 36-37 0.0029793067075815395 38-39 0.0019016851324988552 40-41 0.0 42-43 2.1129834805542833E-5 44-45 7.043278268514278E-6 46-47 1.26779008833257E-4 48-49 7.043278268514279E-4 50-51 0.004359789248210338 52-53 0.012804679892158957 54-55 0.01063535018545656 56-57 8.099770008791421E-4 58-59 0.004275269908988167 60-61 1.4086556537028556E-5 62-63 0.0 64-65 7.043278268514278E-6 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.930294787959995E-5 84-85 4.2259669611085665E-5 86-87 0.0021270700370913122 88-89 0.002014377584795084 90-91 0.001796035958471141 92-93 0.002514450341859597 94-95 6.902412703143992E-4 96-97 2.8173113074057112E-5 98-99 2.7468785247205683E-4 100-101 3.521639134257139E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7098967.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.83968022253671 #Duplication Level Percentage of deduplicated Percentage of total 1 79.55575309967871 45.21923566048821 2 10.75837137463123 12.230047772986634 3 3.5476878843732687 6.0494833463143305 4 1.6409317868381255 3.730801521234993 5 0.927179383402349 2.6350289830759133 6 0.5927466760003669 2.0214918910099455 7 0.42530331885679057 1.6921873248982477 8 0.30854068940802265 1.402988329727442 9 0.2516030537208448 1.2870933404855887 >10 1.9313722810498737 21.245686213718123 >50 0.0581334096754074 2.0596411801934513 >100 0.002228148754948482 0.1537124497023746 >500 7.444932789543038E-5 0.0285213527595107 >1k 4.962952147340982E-5 0.09180704090428987 >5k 0.0 0.0 >10k+ 2.4814760736013148E-5 0.1522735925010433 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10796 0.1520784643737603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.8173113074057112E-5 0.0 0.0 0.0 0.0 3 2.8173113074057112E-5 0.0 0.0 0.0 1.4086556537028556E-5 4 4.225966961108567E-5 0.0 0.0 0.0 5.6346226148114225E-5 5 9.86058957591999E-5 0.0 0.0 0.0 7.043278268514278E-5 6 1.26779008833257E-4 0.0 0.0 0.0 7.043278268514278E-5 7 1.690386784443427E-4 0.0 0.0 0.0 7.043278268514278E-5 8 1.972117915183998E-4 0.0 0.0 1.4086556537028556E-5 8.451933922217134E-5 9 5.916353745551993E-4 0.0 0.0 1.4086556537028556E-5 8.451933922217134E-5 10-11 0.0010564917402771418 0.0 0.0 1.4086556537028556E-5 9.156261749068562E-5 12-13 0.0016622136713693697 0.0 0.0 2.1129834805542836E-5 1.26779008833257E-4 14-15 0.001873512019424798 0.0 0.0 2.8173113074057112E-5 1.8312523498137124E-4 16-17 0.0021059402022857695 0.0 0.0 2.8173113074057112E-5 1.972117915183998E-4 18-19 0.002606012959350283 0.0 0.0 2.8173113074057112E-5 2.1834162632394262E-4 20-21 0.003493466021183082 0.0 0.0 2.8173113074057112E-5 2.3242818286097118E-4 22-23 0.004190750569765996 0.0 0.0 2.8173113074057112E-5 2.6764457420354256E-4 24-25 0.004676736770293481 0.0 0.0 2.8173113074057112E-5 2.6764457420354256E-4 26-27 0.005057073796793252 0.0 0.0 2.8173113074057112E-5 2.887744090090854E-4 28-29 0.005634622614811423 0.0 0.0 2.8173113074057112E-5 3.310340786201711E-4 30-31 0.006106522258801879 7.043278268514278E-6 0.0 2.8173113074057112E-5 3.662504699627425E-4 32-33 0.006444599615690565 1.4086556537028556E-5 0.0 2.8173113074057112E-5 4.0851013957382814E-4 34-35 0.006747460581236679 1.4086556537028556E-5 0.0 4.225966961108567E-5 4.578130874534281E-4 36-37 0.007099624494662392 1.4086556537028556E-5 0.0 4.930294787959995E-5 4.930294787959995E-4 38-39 0.007367269068865935 2.8173113074057112E-5 0.0 5.6346226148114225E-5 4.930294787959995E-4 40-41 0.007606740529995421 2.8173113074057112E-5 0.0 5.6346226148114225E-5 5.352891484070851E-4 42-43 0.007789865764976792 4.225966961108567E-5 0.0 5.6346226148114225E-5 5.916353745551993E-4 44-45 0.008043423782643305 4.225966961108567E-5 0.0 5.6346226148114225E-5 6.268517658977708E-4 46-47 0.008311068356846849 4.225966961108567E-5 0.0 5.6346226148114225E-5 6.620681572403422E-4 48-49 0.008916790287939076 4.225966961108567E-5 0.0 5.6346226148114225E-5 7.113711051199422E-4 50-51 0.0120862655087705 4.225966961108567E-5 0.0 5.6346226148114225E-5 7.465874964625135E-4 52-53 0.012311650413362959 4.225966961108567E-5 0.0 5.6346226148114225E-5 7.958904443421135E-4 54-55 0.012600424822372044 4.225966961108567E-5 0.0 5.6346226148114225E-5 8.029337226106277E-4 56-57 0.012868069396575586 4.225966961108567E-5 0.0 5.6346226148114225E-5 8.029337226106277E-4 58-59 0.013192060196927244 4.225966961108567E-5 0.0 7.043278268514278E-5 8.240635574161706E-4 60-61 0.013649873284380672 4.225966961108567E-5 0.0 7.043278268514278E-5 8.87453061832799E-4 62-63 0.01417811915451924 4.225966961108567E-5 0.0 7.043278268514278E-5 9.085828966383419E-4 64-65 0.014889490259639183 4.225966961108567E-5 0.0 7.043278268514278E-5 9.297127314438847E-4 66-67 0.015713553817055352 4.225966961108567E-5 0.0 7.043278268514278E-5 9.437992879809133E-4 68-69 0.016622136713693697 4.225966961108567E-5 0.0 7.043278268514278E-5 9.931022358605132E-4 70-71 0.01773497468011895 4.225966961108567E-5 0.0 7.043278268514278E-5 0.0010283186272030845 72-73 0.01917884672516438 4.225966961108567E-5 0.0 7.043278268514278E-5 0.001112837966425256 74-75 0.021193224309959464 4.225966961108567E-5 0.0 7.043278268514278E-5 0.0011339678012307988 76-77 0.023996449060828144 4.225966961108567E-5 0.0 7.043278268514278E-5 0.0011480543577678274 78-79 0.02739130918625203 4.225966961108567E-5 0.0 7.043278268514278E-5 0.0011550976360363415 80-81 0.03211030562615659 4.225966961108567E-5 0.0 7.043278268514278E-5 0.001190314027378913 82-83 0.038364736728597276 4.225966961108567E-5 0.0 7.043278268514278E-5 0.00121848714045297 84-85 0.04601373692820378 4.225966961108567E-5 0.0 7.043278268514278E-5 0.0012607468100640559 86-87 0.05597997567815148 4.225966961108567E-5 0.0 7.043278268514278E-5 0.0013452661492862272 88-89 0.06890439130087518 4.930294787959995E-5 0.0 7.043278268514278E-5 0.0013804825406287984 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4385 0.0 86.87451 1 TCGACGG 4950 0.0 76.86258 2 CGACGGC 7030 0.0 54.120876 3 ACGGCGC 7695 0.0 49.44376 5 GACGGCG 7920 0.0 48.099083 4 CGGCGCG 8265 0.0 45.977028 6 TATCTCG 2135 0.0 43.161 2 CGCGCCG 11020 0.0 37.50001 9 GCGCGCC 11780 0.0 35.1613 8 GGCGCGC 11835 0.0 35.07817 7 ATATCTC 3005 0.0 31.61354 1 ATCTCGA 3200 0.0 28.944912 3 TCTCGAG 3900 0.0 23.506084 4 TCGAGGG 4075 0.0 22.03068 6 CCATATT 10490 0.0 20.398836 18-19 AGGGCGC 4455 0.0 20.258142 9 ATCCATA 10765 0.0 19.767284 16-17 CGCCGGA 11545 0.0 19.666529 10-11 CTCGAGG 4720 0.0 19.422401 5 GCCGGAT 11315 0.0 19.184649 12-13 >>END_MODULE