FastQCFastQC Report
Tue 31 May 2016
SRR1567936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7098967
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1084081.5270954210661918No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC229670.32352594398593487No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC188930.2661373126540805No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT103360.14559864836672717No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT90330.12724386519897896No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCG71190.10028219598710629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG637350.094.049691
TCGACGG650650.092.188382
ATATCTC391550.090.248941
TATCTCG396650.088.999442
CGACGGC678850.088.316813
ACGGCGC685150.087.470065
GACGGCG687750.087.160114
CGGCGCG689350.086.8958056
ATCTCGA430350.082.0190053
TCTCGAG443100.078.6405644
TCGAGGG457050.076.1571666
CTCGAGG462350.075.34585
AGGGCGC474600.073.901469
CGAGGGC472350.073.740627
GAGGGCG485200.072.228228
CGCGCCG1150650.053.351029
GGCGCGC1162000.052.9484527
GCGCGCC1160350.052.9050338
CCATATT720700.041.76884518-19
ATCCATA722550.041.6980616-17
Produced by FastQC (version 0.11.5)