##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567936_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7098967 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8426055509203 34.0 31.0 34.0 31.0 34.0 2 33.033071572244246 34.0 31.0 34.0 31.0 34.0 3 33.10923434353195 34.0 33.0 34.0 31.0 34.0 4 36.52513231854719 37.0 37.0 37.0 35.0 37.0 5 36.38902026168033 37.0 37.0 37.0 35.0 37.0 6 36.39917723240578 37.0 37.0 37.0 35.0 37.0 7 36.36723779671042 37.0 37.0 37.0 35.0 37.0 8 36.3575330044498 37.0 37.0 37.0 35.0 37.0 9 38.15273954647204 39.0 39.0 39.0 37.0 39.0 10-11 38.16706747615533 39.0 39.0 39.0 37.0 39.0 12-13 38.14956626788094 39.0 39.0 39.0 37.0 39.0 14-15 39.691964478775574 41.0 40.0 41.0 37.0 41.0 16-17 39.63553894531415 41.0 39.5 41.0 37.0 41.0 18-19 39.70416583145125 41.0 40.0 41.0 37.0 41.0 20-21 39.74781133649445 41.0 40.0 41.0 37.0 41.0 22-23 39.73926734692526 41.0 40.0 41.0 37.5 41.0 24-25 39.720277330490475 41.0 40.0 41.0 37.0 41.0 26-27 39.68940502188558 41.0 40.0 41.0 37.0 41.0 28-29 39.50980213036629 41.0 39.5 41.0 37.0 41.0 30-31 39.026798617319955 40.5 38.0 41.0 35.5 41.0 32-33 38.67584107659607 40.0 36.5 41.0 35.0 41.0 34-35 38.56439281940598 40.0 36.0 41.0 35.0 41.0 36-37 38.489922547886195 40.0 36.0 41.0 35.0 41.0 38-39 38.23171913603768 40.0 36.0 41.0 34.5 41.0 40-41 37.54836175460458 40.0 36.0 41.0 32.0 41.0 42-43 37.72297863900481 40.0 36.0 41.0 33.0 41.0 44-45 37.663197617343485 40.0 35.5 41.0 32.5 41.0 46-47 37.498609868168145 40.0 35.0 41.0 32.0 41.0 48-49 37.096697237781214 40.0 35.0 41.0 30.5 41.0 50-51 36.94456989305627 40.0 35.0 41.0 30.0 41.0 52-53 36.92740007102442 40.0 35.0 41.0 31.0 41.0 54-55 36.70989884866347 39.5 35.0 41.0 31.0 41.0 56-57 36.41902976869733 39.0 35.0 41.0 30.0 41.0 58-59 36.116247053972785 39.0 35.0 41.0 30.0 41.0 60-61 35.79156587148525 38.0 35.0 41.0 29.5 41.0 62-63 35.43627741895405 37.0 35.0 40.0 29.0 41.0 64-65 35.05773896399293 37.0 35.0 40.0 29.0 41.0 66-67 34.71397796327268 36.0 35.0 39.0 29.0 41.0 68-69 34.337871481864894 36.0 34.0 39.0 29.0 41.0 70-71 33.96388601327489 35.0 34.0 38.5 28.0 40.0 72-73 33.62787219605332 35.0 34.0 37.0 28.0 39.5 74-75 33.30392816306936 35.0 34.0 37.0 27.0 39.0 76-77 33.00453558947379 35.0 34.0 36.0 27.0 39.0 78-79 32.755519429798724 35.0 34.0 36.0 27.0 37.0 80-81 32.536429102994845 35.0 34.0 35.5 27.0 37.0 82-83 32.33856566737104 35.0 34.0 35.0 27.0 36.5 84-85 32.16644062438944 35.0 34.0 35.0 26.0 36.0 86-87 32.012711286022316 35.0 33.5 35.0 26.0 36.0 88-89 31.893856740001752 35.0 33.0 35.0 26.0 36.0 90-91 31.780870512568942 35.0 33.0 35.0 25.0 35.0 92-93 31.670933320298573 35.0 33.0 35.0 25.0 35.0 94-95 31.565060592618615 35.0 33.0 35.0 25.0 35.0 96-97 31.428969454288207 35.0 33.0 35.0 25.0 35.0 98-99 31.277208782629923 35.0 33.0 35.0 24.5 35.0 100-101 30.646591187140324 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17948205649787496 1101 2 0.19017582941870614 1101 3 0.1733902378131873 1101 4 0.10069616640516443 1101 5 0.1277919745128031 1101 6 0.13420133654887678 1101 7 0.1068424779143129 1101 8 0.11373256649832086 1101 9 0.17857917448702665 1101 10-11 0.17920376861954423 1101 12-13 0.13816493795146556 1101 14-15 0.17801568531331924 1101 16-17 0.2422271418928048 1101 18-19 0.23405046134468677 1101 20-21 0.23336906416231074 1101 22-23 0.21296251169805913 1101 24-25 0.21857227812255786 1101 26-27 0.2528530588540292 1101 28-29 0.2658586055557919 1101 30-31 0.2919332631326057 1101 32-33 0.28867909075015774 1101 34-35 0.34381232546835605 1101 36-37 0.3325493496338723 1101 38-39 0.35795479372005445 1101 40-41 0.12585075919582778 1101 42-43 0.18926880627663678 1101 44-45 0.16989249528145223 1101 46-47 0.22857088415968718 1101 48-49 0.2621958691010775 1101 50-51 0.29512317285063716 1101 52-53 0.23628476410024746 1101 54-55 0.22070132786945607 1101 56-57 0.2354422324962684 1101 58-59 0.27717650021247664 1101 60-61 0.2805353668497048 1101 62-63 0.23275381910568171 1101 64-65 0.2396063016609915 1101 66-67 0.20111678057278226 1101 68-69 0.24800105901280034 1101 70-71 0.22511703676731543 1101 72-73 0.23748969939167353 1101 74-75 0.18978467184441428 1101 76-77 0.1713999733067979 1101 78-79 0.1341090583504112 1101 80-81 0.10555809970563956 1101 82-83 0.1393720731278023 1101 84-85 0.16861458753507463 1101 86-87 0.13889250727209657 1101 88-89 0.1360405789456287 1101 90-91 0.11655857541080294 1101 92-93 0.10894762954536219 1101 94-95 0.1607053847672013 1101 96-97 0.13939093885096554 1101 98-99 0.1336200062292221 1101 100-101 0.2283124218179715 1102 1 0.1967013749981561 1102 2 0.20753356691037794 1102 3 0.1569863734845498 1102 4 0.11654242613080612 1102 5 0.12124644150449626 1102 6 0.1542452849060325 1102 7 0.10333738791124603 1102 8 0.14320271620761105 1102 9 0.16552109895121703 1102 10-11 0.18482665609550963 1102 12-13 0.18431095976285405 1102 14-15 0.30478498746441574 1102 16-17 0.22698388771579658 1102 18-19 0.2447061348108548 1102 20-21 0.2202406509067245 1102 22-23 0.22257645658793734 1102 24-25 0.2189081413640679 1102 26-27 0.23940721169575596 1102 28-29 0.2506624648074194 1102 30-31 0.2599404698653629 1102 32-33 0.3014482552982187 1102 34-35 0.2952907120192805 1102 36-37 0.3058615264153062 1102 38-39 0.2985669368763766 1102 40-41 0.13213447874107942 1102 42-43 0.15107248803444406 1102 44-45 0.15555764784454595 1102 46-47 0.1456805181255163 1102 48-49 0.21911777564665158 1102 50-51 0.2766685497207959 1102 52-53 0.16027230387266655 1102 54-55 0.12693165823966268 1102 56-57 0.15557614161338051 1102 58-59 0.1887062703186828 1102 60-61 0.12896000843205258 1102 62-63 0.10029322585726419 1102 64-65 0.13130411369201767 1102 66-67 0.08428026749434281 1102 68-69 0.13134694048117979 1102 70-71 0.10326628791865744 1102 72-73 0.10178763778379363 1102 74-75 0.09203352966864031 1102 76-77 0.09397008084871317 1102 78-79 0.0674510313282326 1102 80-81 0.09645419772251529 1102 82-83 0.1036019554318699 1102 84-85 0.10646213847288521 1102 86-87 0.13325606096034193 1102 88-89 0.11240745767141291 1102 90-91 0.14073287656161426 1102 92-93 0.10850022825842132 1102 94-95 0.14380272955227724 1102 96-97 0.14120437269562913 1102 98-99 0.1391620896924195 1102 100-101 0.23043674687302484 1103 1 0.15008345087059638 1103 2 0.13547608536374867 1103 3 0.1441680685454969 1103 4 0.06735032622026438 1103 5 0.0893931089041331 1103 6 0.08986675227967567 1103 7 0.0844212513988225 1103 8 0.11108386503893541 1103 9 0.14359110429857225 1103 10-11 0.14722486898013187 1103 12-13 0.12698805959843895 1103 14-15 0.23513741301376712 1103 16-17 0.20885766019842578 1103 18-19 0.23112535729572414 1103 20-21 0.17002233397360556 1103 22-23 0.1889963037635951 1103 24-25 0.2113099774552083 1103 26-27 0.21765080611651655 1103 28-29 0.1945043130925228 1103 30-31 0.23410973108106958 1103 32-33 0.2738692319147944 1103 34-35 0.287286474117046 1103 36-37 0.30289207129558804 1103 38-39 0.28501477019888455 1103 40-41 0.10342046717680375 1103 42-43 0.06846462892470839 1103 44-45 0.08611914490349903 1103 46-47 0.1960531586684482 1103 48-49 0.14121283859049072 1103 50-51 0.17721697824867988 1103 52-53 0.20152880690366004 1103 54-55 0.2330671961483901 1103 56-57 0.2087938238606739 1103 58-59 0.24677745008469998 1103 60-61 0.17919531695434188 1103 62-63 0.1706511236978301 1103 64-65 0.23033174501450304 1103 66-67 0.21616336036337458 1103 68-69 0.19427709005713467 1103 70-71 0.19419133002281797 1103 72-73 0.2053113185618045 1103 74-75 0.20697835697912836 1103 76-77 0.19571184367611494 1103 78-79 0.18864159200070674 1103 80-81 0.1676548533979556 1103 82-83 0.1708150099698713 1103 84-85 0.14949586177984742 1103 86-87 0.15062186488740537 1103 88-89 0.15741602842888724 1103 90-91 0.14041935675392025 1103 92-93 0.17319980196943874 1103 94-95 0.2035310390390812 1103 96-97 0.24725355335120724 1103 98-99 0.26221270463801716 1103 100-101 0.2952697545342353 1104 1 0.1598180181531461 1104 2 0.16772039500349223 1104 3 0.15977133058537873 1104 4 0.05400350028867251 1104 5 0.09077216226555151 1104 6 0.0830523219805741 1104 7 0.08288227155038896 1104 8 0.08363383710439365 1104 9 0.1402766586181059 1104 10-11 0.15345651429977636 1104 12-13 0.14998353053113078 1104 14-15 0.21240067646279215 1104 16-17 0.21106221149427284 1104 18-19 0.22313473893513702 1104 20-21 0.2138636310492572 1104 22-23 0.21067577500193124 1104 24-25 0.21865201829421466 1104 26-27 0.22302512400265329 1104 28-29 0.2472823659706691 1104 30-31 0.2157875599867154 1104 32-33 0.2496707448455524 1104 34-35 0.2368358795529133 1104 36-37 0.24758157614468246 1104 38-39 0.25869658734325185 1104 40-41 0.06188251440651982 1104 42-43 0.11593221146060273 1104 44-45 0.09803739444479476 1104 46-47 0.13329265046819927 1104 48-49 0.1959885286927303 1104 50-51 0.2527824368663545 1104 52-53 0.2528562979651028 1104 54-55 0.2293902762317046 1104 56-57 0.1885160165881672 1104 58-59 0.19224404622864455 1104 60-61 0.18526465911822498 1104 62-63 0.1352156971428471 1104 64-65 0.12729528026710568 1104 66-67 0.09963092503704729 1104 68-69 0.08487853610377272 1104 70-71 0.1124622365209973 1104 72-73 0.1309280913929598 1104 74-75 0.1317684112902171 1104 76-77 0.10301682535244794 1104 78-79 0.09524615599858777 1104 80-81 0.04855910391373186 1104 82-83 0.12704976629434128 1104 84-85 0.13868069416763973 1104 86-87 0.056848131691545234 1104 88-89 0.08542153197999625 1104 90-91 0.11027835430580168 1104 92-93 0.12540046326883214 1104 94-95 0.151556617750483 1104 96-97 0.13644202268721628 1104 98-99 0.16858229101826794 1104 100-101 0.2732562298501229 1105 1 0.15487918773290943 1105 2 0.16105384679643464 1105 3 0.1597808774945264 1105 4 0.07979266338557522 1105 5 0.0764680088067422 1105 6 0.07101729347467511 1105 7 0.07958722405042806 1105 8 0.07695380632360127 1105 9 0.07341860175156967 1105 10-11 0.09577948615829968 1105 12-13 0.11387943867131156 1105 14-15 0.17731767524499276 1105 16-17 0.16942854985637723 1105 18-19 0.18433343092286236 1105 20-21 0.1650501092279839 1105 22-23 0.20621614097041885 1105 24-25 0.21150767650956936 1105 26-27 0.20423606642832937 1105 28-29 0.19314488362076077 1105 30-31 0.2236658642499023 1105 32-33 0.2866719733963734 1105 34-35 0.24629006678051013 1105 36-37 0.259635172414697 1105 38-39 0.29448648847467496 1105 40-41 0.20183994921983128 1105 42-43 0.18681832361473028 1105 44-45 0.19174476944379393 1105 46-47 0.1385085360885867 1105 48-49 0.1795743134774952 1105 50-51 0.22695042957921885 1105 52-53 0.19104193165152594 1105 54-55 0.22657485375720654 1105 56-57 0.1981158234054874 1105 58-59 0.24668588014547055 1105 60-61 0.20862397796924625 1105 62-63 0.2172001301476314 1105 64-65 0.21682325224330867 1105 66-67 0.17618020524685818 1105 68-69 0.16131264941569157 1105 70-71 0.1552714733777094 1105 72-73 0.23089691391537315 1105 74-75 0.20046300122437088 1105 76-77 0.18587392378723422 1105 78-79 0.15213899322932178 1105 80-81 0.1390968076584116 1105 82-83 0.14202869139498375 1105 84-85 0.19205824254942172 1105 86-87 0.15199738222410275 1105 88-89 0.17658997468349824 1105 90-91 0.2032874216094065 1105 92-93 0.18008870485909867 1105 94-95 0.21997477595609283 1105 96-97 0.28306620054247134 1105 98-99 0.21632320785510473 1105 100-101 0.20052337528064257 1106 1 0.1283362123456797 1106 2 0.05190419716797834 1106 3 0.08399921123405107 1106 4 0.07497857788904128 1106 5 0.09131979171026217 1106 6 0.08105586809772802 1106 7 0.07466934100926181 1106 8 0.06762653951147257 1106 9 0.10011814113685347 1106 10-11 0.12079918537997969 1106 12-13 0.13524247225229402 1106 14-15 0.17264085499304826 1106 16-17 0.11642459971428565 1106 18-19 0.16619760850799992 1106 20-21 0.16187821034888117 1106 22-23 0.15573474004872878 1106 24-25 0.11937387321725623 1106 26-27 0.1513755983229501 1106 28-29 0.11473627942790188 1106 30-31 0.12874082617681637 1106 32-33 0.17448922526151023 1106 34-35 0.18520130243086896 1106 36-37 0.22047161319060393 1106 38-39 0.178633935528012 1106 40-41 0.043421268484145514 1106 42-43 0.09316553488764612 1106 44-45 0.08594261446717866 1106 46-47 0.09203637492187511 1106 48-49 0.10547149574507131 1106 50-51 0.1344677898758846 1106 52-53 0.1324630827039428 1106 54-55 0.11860112180163895 1106 56-57 0.10336280549616106 1106 58-59 0.10702269869637604 1106 60-61 0.1597327027983937 1106 62-63 0.1311176302649102 1106 64-65 0.10135297480274374 1106 66-67 0.0018170450416477024 1106 68-69 0.06110714248205795 1106 70-71 0.02985793274677917 1106 72-73 1.522833358507114E-4 1106 74-75 -0.03251834916205354 1106 76-77 0.0393963276948881 1106 78-79 0.03579736372038411 1106 80-81 0.035739291766496706 1106 82-83 0.046678127249698775 1106 84-85 0.07358816267318247 1106 86-87 0.07906745004816429 1106 88-89 0.0360116280374605 1106 90-91 0.0428393777821654 1106 92-93 0.024464386370169677 1106 94-95 0.052696222477759846 1106 96-97 0.03846496008267053 1106 98-99 0.07690178524710944 1106 100-101 0.15720459610605886 1107 1 0.03993937190451646 1107 2 0.06546940055485351 1107 3 0.0688645023658907 1107 4 0.004678393883800425 1107 5 0.05955861718666 1107 6 0.055030696032467574 1107 7 0.04843137817314158 1107 8 0.03741395236814071 1107 9 0.0890782893776958 1107 10-11 0.06885755058411291 1107 12-13 0.07324205389027583 1107 14-15 0.10615350396509626 1107 16-17 0.05126286391915613 1107 18-19 0.06944306113497589 1107 20-21 0.06764906088256595 1107 22-23 0.0782140625984411 1107 24-25 0.04856031643664949 1107 26-27 0.03462626196358798 1107 28-29 -0.005499669346818337 1107 30-31 0.032047135668456406 1107 32-33 0.08982691375581453 1107 34-35 0.0833220979851248 1107 36-37 0.12893895503680142 1107 38-39 0.1255797076651035 1107 40-41 0.07825657083400728 1107 42-43 0.05566132350005404 1107 44-45 0.05969720071454532 1107 46-47 0.05678590380181703 1107 48-49 0.030480520147051493 1107 50-51 0.09643812689796505 1107 52-53 -0.033914263177784676 1107 54-55 0.025278161131375043 1107 56-57 0.03980496046781923 1107 58-59 0.040461301293184704 1107 60-61 0.014397994670694914 1107 62-63 0.004053217323082947 1107 64-65 0.015669517503440034 1107 66-67 0.0518692477361995 1107 68-69 -0.04341570267585837 1107 70-71 -0.02850343022485191 1107 72-73 -0.08253757157028474 1107 74-75 -0.06468104015541343 1107 76-77 -0.036825159822747366 1107 78-79 -0.032717747293645516 1107 80-81 -0.07997746789008175 1107 82-83 -0.06709857109647288 1107 84-85 -0.027382261353380954 1107 86-87 -0.001572245843377118 1107 88-89 -0.029224570643773262 1107 90-91 -0.02782381857771199 1107 92-93 -0.048880121795296105 1107 94-95 -0.08254559447219734 1107 96-97 -0.024887221138428117 1107 98-99 -0.004327551186300127 1107 100-101 0.09511458162999276 1108 1 0.034152988910250315 1108 2 0.07671521212897403 1108 3 0.09096924364818904 1108 4 0.03860554766429658 1108 5 0.04901882068894281 1108 6 -0.03608762026158985 1108 7 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5 -0.0996165751708773 2214 6 -0.07421936844755095 2214 7 -0.09726239227298805 2214 8 -0.07938771965669389 2214 9 -0.14207539458049467 2214 10-11 -0.13873613213154812 2214 12-13 -0.14425997150387104 2214 14-15 -0.12288973812142956 2214 16-17 -0.1807504123747279 2214 18-19 -0.19754765813566166 2214 20-21 -0.18851727590580225 2214 22-23 -0.2238824870461471 2214 24-25 -0.2015927961438848 2214 26-27 -0.19794646082505807 2214 28-29 -0.21832570132514206 2214 30-31 -0.20989200743450454 2214 32-33 -0.19620283992738763 2214 34-35 -0.23402184939667592 2214 36-37 -0.16916949783819035 2214 38-39 -0.17021079849203602 2214 40-41 -0.04119047616957516 2214 42-43 -0.03776031869140439 2214 44-45 -0.08556424770426219 2214 46-47 -0.029308071034982675 2214 48-49 -0.07123738590603068 2214 50-51 -0.12840798393737884 2214 52-53 -0.12190903355048732 2214 54-55 -0.11747841128046588 2214 56-57 -0.16809107233257947 2214 58-59 -0.19635226894170899 2214 60-61 -0.16756495269667226 2214 62-63 -0.03180138918566655 2214 64-65 -0.058445231507711526 2214 66-67 -0.0548164355668348 2214 68-69 -0.026306111124597464 2214 70-71 -0.06274049045075003 2214 72-73 -0.05922786574348038 2214 74-75 0.00132862588517213 2214 76-77 0.009599697897634485 2214 78-79 0.015814022370562952 2214 80-81 -0.018103222117986206 2214 82-83 -0.03740381415317984 2214 84-85 -0.014929076040644418 2214 86-87 -0.039478548380465384 2214 88-89 -0.055399934634245085 2214 90-91 -0.051667429910384755 2214 92-93 -0.1356213206654644 2214 94-95 -0.13615136083678792 2214 96-97 -0.13243757202894102 2214 98-99 -0.07999911603311105 2214 100-101 -0.18388716419386597 2215 1 -0.17725283737844677 2215 2 -0.1770531482955917 2215 3 -0.15484415380905148 2215 4 -0.12189227172773087 2215 5 -0.10614742915438313 2215 6 -0.12830622819382143 2215 7 -0.13682858591491964 2215 8 -0.15507954563134518 2215 9 -0.2053828297583422 2215 10-11 -0.19314049226630914 2215 12-13 -0.22043902091442646 2215 14-15 -0.214512679700114 2215 16-17 -0.3355131345222233 2215 18-19 -0.27029591331803715 2215 20-21 -0.28549933544120165 2215 22-23 -0.3210977017390064 2215 24-25 -0.2592694976710277 2215 26-27 -0.21278216349082157 2215 28-29 -0.21694054036345278 2215 30-31 -0.2722726972624301 2215 32-33 -0.31218178057141444 2215 34-35 -0.3425679466142171 2215 36-37 -0.334300174193487 2215 38-39 -0.28366543393953947 2215 40-41 -0.11214723825274575 2215 42-43 -0.1804272184348008 2215 44-45 -0.11881079681383966 2215 46-47 -0.15066909485668845 2215 48-49 -0.2746777825244209 2215 50-51 -0.34003869068858705 2215 52-53 -0.3011546483925187 2215 54-55 -0.23116130364038412 2215 56-57 -0.20435587495003915 2215 58-59 -0.19082174508489658 2215 60-61 -0.23697201003586343 2215 62-63 -0.22695616085470505 2215 64-65 -0.20087991064874444 2215 66-67 -0.17979524632730914 2215 68-69 -0.14401896516396562 2215 70-71 -0.09123697377221873 2215 72-73 -0.15432490804914778 2215 74-75 -0.14086237618440123 2215 76-77 -0.12376929034635253 2215 78-79 -0.09910753127094551 2215 80-81 -0.07425044907801492 2215 82-83 -0.11834857824814549 2215 84-85 -0.1266012044956213 2215 86-87 -0.10934574993157042 2215 88-89 -0.08798973332101312 2215 90-91 -0.09329230137801403 2215 92-93 -0.16710194623390606 2215 94-95 -0.12850815101934998 2215 96-97 -0.15458750804851462 2215 98-99 -0.14881402488725826 2215 100-101 -0.16952183029556522 2216 1 -0.2777653352326368 2216 2 -0.2421230093389113 2216 3 -0.2508214493880132 2216 4 -0.1501763982542883 2216 5 -0.1655839890562376 2216 6 -0.21359519942934924 2216 7 -0.20161510886870104 2216 8 -0.23392142032550822 2216 9 -0.30730632923268075 2216 10-11 -0.3083536531516273 2216 12-13 -0.2608182843588267 2216 14-15 -0.35027762651699135 2216 16-17 -0.4064519434589542 2216 18-19 -0.40369115316227777 2216 20-21 -0.43688968144569174 2216 22-23 -0.43889696914903453 2216 24-25 -0.383509500478894 2216 26-27 -0.4112893272244875 2216 28-29 -0.3939017179769593 2216 30-31 -0.4239143170557398 2216 32-33 -0.6111758682936212 2216 34-35 -0.5272893486971313 2216 36-37 -0.5865745502075441 2216 38-39 -0.5673040228634463 2216 40-41 -0.40555233735229024 2216 42-43 -0.339509721225177 2216 44-45 -0.43057323385790625 2216 46-47 -0.553700779660879 2216 48-49 -0.6827527336203261 2216 50-51 -0.750834504566555 2216 52-53 -0.7395822957634124 2216 54-55 -0.6707814068398577 2216 56-57 -0.574857887088335 2216 58-59 -0.6694977956277413 2216 60-61 -0.5911696355606182 2216 62-63 -0.5257531565607465 2216 64-65 -0.464099521905986 2216 66-67 -0.3861180316434769 2216 68-69 -0.38019662262821186 2216 70-71 -0.4582793254383688 2216 72-73 -0.3981345327799488 2216 74-75 -0.3400770490566529 2216 76-77 -0.45567619326968867 2216 78-79 -0.3334554980270923 2216 80-81 -0.2945968493723967 2216 82-83 -0.33274668874372537 2216 84-85 -0.3036347422716261 2216 86-87 -0.3161078678841349 2216 88-89 -0.2935327786276254 2216 90-91 -0.2831804776464395 2216 92-93 -0.2616774802277675 2216 94-95 -0.35391490132695225 2216 96-97 -0.39617236161757674 2216 98-99 -0.38168580985068346 2216 100-101 -0.414259509665591 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 12.0 13 121.0 14 474.0 15 1358.0 16 2774.0 17 6092.0 18 16396.0 19 66461.0 20 71866.0 21 51790.0 22 44169.0 23 41586.0 24 40713.0 25 44211.0 26 53772.0 27 70751.0 28 88835.0 29 103322.0 30 117597.0 31 137209.0 32 168372.0 33 220507.0 34 309310.0 35 459357.0 36 687832.0 37 1734168.0 38 2187845.0 39 371886.0 40 181.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.005386617683826 28.143938704890655 21.332029794134158 23.51864488329136 2 21.18094083265917 23.753455960564406 35.673570534980655 19.39203267179577 3 20.374161480113937 27.244851257936542 21.718286618320665 30.662700643628853 4 30.0267630487647 21.01328263675546 26.70177224376448 22.25818207071536 5 22.241207770088238 30.389773610723925 21.647079075025985 25.72193954416185 6 22.57925695386385 21.37756098880302 25.524699579530374 30.518482477802756 7 31.575706719019824 21.795692246491637 20.77569877420194 25.852902260286605 8 36.13777891910189 21.66640583059479 20.996082951223748 21.19973229907957 9 21.690606534725404 26.256172200828654 21.33678040762832 30.716440856817616 10-11 31.370944533197576 21.426441339986507 20.97019186030869 26.23242226650723 12-13 28.739864546489652 21.527286998235095 20.996005475162796 28.73684298011246 14-15 28.452350884290627 21.80042532948808 21.236723596545808 28.510500189675486 16-17 28.3263043763973 26.11344016671722 21.42665968161283 24.133595775272656 18-19 23.678212618821863 21.885585607032684 26.08425282157249 28.351948952572958 20-21 23.35074948228383 28.643970312863832 22.119957734695767 25.885322470156574 22-23 20.743102482375253 25.891428992415378 29.538283527730165 23.82718499747921 24-25 20.787038452214247 21.397296254511396 34.25871679640151 23.556948496872852 26-27 20.79264490171598 21.426194825247112 36.96008870022921 20.821071572807707 28-29 20.8286783133377 21.376074857088362 36.99149467802851 20.803752151545428 30-31 20.80750621886255 21.318150936608102 36.994657109971065 20.879685734558283 32-33 20.8438002317802 21.37512401452211 37.011201770623806 20.769873983073875 34-35 20.853998898713012 21.44919112879381 36.91132808477628 20.7854818877169 36-37 20.90430903538501 21.391879973522908 36.789958313653244 20.913852677438843 38-39 21.19499217280486 21.62468849341038 35.857245145666965 21.32307418811779 40-41 22.779250840298314 22.90050791896906 31.17033788155375 23.14990335917888 42-43 22.661254799465894 23.203354797958635 31.74640056785727 22.388989834718206 44-45 23.010291497340386 23.859816505697236 30.73175998705164 22.398132009910736 46-47 22.831427445711466 24.58990864445489 29.878276656307882 22.700387253525758 48-49 23.070877777006146 25.168197006691255 28.247588698468384 23.513336517834215 50-51 23.221174291977974 25.459838029955627 27.64412061642208 23.67486706164432 52-53 23.31377227137413 25.279389240716288 27.737514486262576 23.669324001647 54-55 23.39319932040817 24.8651670024667 27.87702069892704 23.864612978198096 56-57 23.34862241224674 24.774477751481307 28.082691467645926 23.794208368626027 58-59 23.377577329208602 24.777273932953907 28.150313982301935 23.69483475553556 60-61 23.44470681438581 24.824118776717796 27.835282231907826 23.895892176988568 62-63 23.695179876170716 24.947023982503367 27.417425662071683 23.940370479254234 64-65 23.868134617332355 25.131740998373424 26.94497664237628 24.055147741917942 66-67 23.976911006911287 25.25119499780743 26.554271910265253 24.21762208501603 68-69 24.056507094623765 25.46538108995295 26.240205088993935 24.237906726429355 70-71 24.191294310848324 25.6489359649087 25.92315896101503 24.236610763227944 72-73 24.294872761065093 25.62472117422154 25.79368237660493 24.286723688108424 74-75 24.28301188046092 25.7060006054402 25.76317089514573 24.247816618953152 76-77 24.26556568018981 25.736455740673254 25.675313042024285 24.322665537112652 78-79 24.329582036372337 25.5917586319249 25.6728267647955 24.40583256690727 80-81 24.283955679748896 25.639582491368113 25.71131123725466 24.365150591628332 82-83 24.351289419995894 25.62075580855637 25.6696502462964 24.358304525151336 84-85 24.374504065168917 25.523868472694687 25.66784716705966 24.433780295076733 86-87 24.446563845134087 25.492363888999627 25.643357688520034 24.417714577346253 88-89 24.49875453710378 25.518959307741536 25.55017511702759 24.432111038127097 90-91 24.574568384386065 25.43374972724905 25.462993418619924 24.528688469744964 92-93 24.486506276194834 25.50959174764441 25.426805054876294 24.577096921284465 94-95 24.47045464502089 25.57022733025805 25.315267700216104 24.64405032450496 96-97 24.44229561850337 25.503330273263703 25.301955904288608 24.75241820394432 98-99 24.46191114848118 25.531003313580698 25.32171934311006 24.685366194828067 100-101 24.450036181320463 25.463451231707374 25.244215109043328 24.84229747792883 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4063.0 1 2431.5 2 623.0 3 375.0 4 275.5 5 208.0 6 177.0 7 183.0 8 179.5 9 162.5 10 157.0 11 153.5 12 152.0 13 195.5 14 264.5 15 476.0 16 4885.0 17 7683.5 18 6942.0 19 7151.0 20 6539.0 21 6530.5 22 7061.5 23 8451.0 24 10116.0 25 12096.5 26 15176.0 27 19529.0 28 25601.0 29 33444.0 30 43240.0 31 54094.0 32 65703.5 33 78820.5 34 92922.5 35 107513.0 36 123364.0 37 140964.5 38 159938.0 39 176048.5 40 187124.5 41 194743.0 42 203940.0 43 215535.0 44 228901.5 45 247588.5 46 268250.0 47 285852.0 48 298895.0 49 306176.5 50 304078.5 51 298596.5 52 298954.0 53 300592.0 54 293248.5 55 278071.5 56 258716.5 57 236673.5 58 214805.0 59 191960.0 60 166193.0 61 141214.5 62 116290.0 63 88937.5 64 65419.5 65 47635.0 66 34639.0 67 26056.0 68 19642.0 69 15135.5 70 11663.5 71 8636.0 72 6669.5 73 4947.0 74 3674.5 75 2786.5 76 2073.0 77 1389.0 78 737.0 79 344.0 80 176.0 81 92.5 82 39.5 83 7.0 84 1.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10570552025386229 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7098967.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.46929699745111 #Duplication Level Percentage of deduplicated Percentage of total 1 79.29627868261474 43.98508833038609 2 11.224315795993443 12.452098129622843 3 3.5605561044441223 5.925046321004957 4 1.6397118814165133 3.638146613621677 5 0.9478884312086359 2.628935245557992 6 0.5930305064279312 1.9736991173759857 7 0.43827761291972256 1.7017665754864606 8 0.30029655030217683 1.3325790828817174 9 0.2355201645653371 1.1757724161446923 >10 1.7010286162334722 17.733910713170747 >50 0.05298941610583399 1.8668401624260815 >100 0.007954797462208348 0.8965697429718607 >500 8.859073104454918E-4 0.366333685870102 >1k 8.858730987436016E-4 1.034019861524202 >5k 2.78417257611103E-4 1.034370848834117 >10k+ 1.0124263913131019E-4 2.254823153120523 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 108408 1.5270954210661918 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 22967 0.32352594398593487 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 18893 0.2661373126540805 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 10336 0.14559864836672717 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT 9033 0.12724386519897896 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCG 7119 0.10028219598710629 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.8173113074057112E-5 0.0 0.0 0.0 0.0 3 4.225966961108567E-5 1.4086556537028556E-5 0.0 0.0 0.0 4 7.043278268514278E-5 1.4086556537028556E-5 0.0 0.0 1.4086556537028556E-5 5 1.4086556537028555E-4 1.4086556537028556E-5 0.0 0.0 1.4086556537028556E-5 6 1.690386784443427E-4 1.4086556537028556E-5 0.0 0.0 1.4086556537028556E-5 7 1.972117915183998E-4 1.4086556537028556E-5 0.0 0.0 1.4086556537028556E-5 8 2.253849045924569E-4 1.4086556537028556E-5 0.0 0.0 2.8173113074057112E-5 9 6.057219310922279E-4 1.4086556537028556E-5 0.0 0.0 2.8173113074057112E-5 10-11 9.719724010549705E-4 1.4086556537028556E-5 0.0 0.0 2.8173113074057112E-5 12-13 0.001549521219073141 1.4086556537028556E-5 0.0 0.0 4.225966961108567E-5 14-15 0.0017256031757859982 1.4086556537028556E-5 0.0 0.0 6.33895044166285E-5 16-17 0.001972117915183998 1.4086556537028556E-5 0.0 0.0 9.156261749068562E-5 18-19 0.0025426234549336545 1.4086556537028556E-5 0.0 0.0 1.1269245229622845E-4 20-21 0.0034793794646460537 1.4086556537028556E-5 0.0 0.0 1.549521219073141E-4 22-23 0.004183707291497481 1.4086556537028556E-5 1.4086556537028556E-5 0.0 1.8312523498137124E-4 24-25 0.004690823326830509 1.4086556537028556E-5 1.4086556537028556E-5 7.043278268514278E-6 1.972117915183998E-4 26-27 0.005085246909867308 1.4086556537028556E-5 1.4086556537028556E-5 1.4086556537028556E-5 2.1834162632394262E-4 28-29 0.005641665893079937 2.1129834805542836E-5 1.4086556537028556E-5 1.4086556537028556E-5 2.3242818286097118E-4 30-31 0.006099478980533365 2.8173113074057112E-5 1.4086556537028556E-5 1.4086556537028556E-5 2.53558017666514E-4 32-33 0.006430513059153537 2.8173113074057112E-5 1.4086556537028556E-5 1.4086556537028556E-5 2.7468785247205683E-4 34-35 0.006747460581236679 2.8173113074057112E-5 1.4086556537028556E-5 1.4086556537028556E-5 3.0286096554611394E-4 36-37 0.007134840886004964 4.225966961108567E-5 1.4086556537028556E-5 2.1129834805542836E-5 3.5216391342571393E-4 38-39 0.007402485460208507 4.930294787959995E-5 1.4086556537028556E-5 2.8173113074057112E-5 4.225966961108567E-4 40-41 0.007634913643069478 5.6346226148114225E-5 1.4086556537028556E-5 3.5216391342571396E-5 4.3668325264788524E-4 42-43 0.007796909043245306 5.6346226148114225E-5 1.4086556537028556E-5 5.6346226148114225E-5 4.578130874534281E-4 44-45 0.00805046706091182 5.6346226148114225E-5 1.4086556537028556E-5 5.6346226148114225E-5 4.859862005274852E-4 46-47 0.00833219819165239 6.33895044166285E-5 1.4086556537028556E-5 5.6346226148114225E-5 5.212025918700566E-4 48-49 0.00892383356620759 7.043278268514278E-5 1.4086556537028556E-5 5.6346226148114225E-5 5.705055397496564E-4 50-51 0.011741144873613302 7.043278268514278E-5 1.4086556537028556E-5 5.6346226148114225E-5 5.775488180181708E-4 52-53 0.011959486499937244 7.043278268514278E-5 1.4086556537028556E-5 5.6346226148114225E-5 5.775488180181708E-4 54-55 0.012220087795872272 8.451933922217134E-5 1.4086556537028556E-5 5.6346226148114225E-5 5.845920962866851E-4 56-57 0.012438429422196215 8.451933922217134E-5 1.4086556537028556E-5 6.33895044166285E-5 6.127652093607422E-4 58-59 0.012727203831205302 8.451933922217134E-5 1.4086556537028556E-5 7.043278268514278E-5 6.409383224347993E-4 60-61 0.013156843805584672 8.451933922217134E-5 1.4086556537028556E-5 7.043278268514278E-5 6.831979920458851E-4 62-63 0.013649873284380672 8.451933922217134E-5 1.4086556537028556E-5 7.043278268514278E-5 7.32500939925485E-4 64-65 0.014354201111232099 8.451933922217134E-5 1.4086556537028556E-5 7.043278268514278E-5 8.029337226106277E-4 66-67 0.015199394503453813 8.451933922217134E-5 1.4086556537028556E-5 8.451933922217134E-5 8.311068356846848E-4 68-69 0.016044587895675526 9.86058957591999E-5 1.4086556537028556E-5 8.451933922217134E-5 8.311068356846848E-4 70-71 0.01715742586210078 9.86058957591999E-5 2.1129834805542836E-5 9.86058957591999E-5 8.451933922217134E-4 72-73 0.01851677856792404 9.86058957591999E-5 2.8173113074057112E-5 9.86058957591999E-5 8.733665052957705E-4 74-75 0.020467766648302493 9.86058957591999E-5 2.8173113074057112E-5 9.86058957591999E-5 8.944963401013133E-4 76-77 0.02321464517302306 9.86058957591999E-5 2.8173113074057112E-5 9.86058957591999E-5 9.226694531753704E-4 78-79 0.02647568301134517 9.86058957591999E-5 2.8173113074057112E-5 9.86058957591999E-5 9.790156793234846E-4 80-81 0.03100451093799985 9.86058957591999E-5 2.8173113074057112E-5 1.0564917402771417E-4 0.0010424051837401131 82-83 0.0373434613796627 9.86058957591999E-5 2.8173113074057112E-5 1.1269245229622845E-4 0.0011057946881567417 84-85 0.04496428846619516 9.86058957591999E-5 2.8173113074057112E-5 1.1269245229622845E-4 0.0011198812446937701 86-87 0.05501504655536503 9.86058957591999E-5 2.8173113074057112E-5 1.1269245229622845E-4 0.001141011079499313 88-89 0.0680239815173109 9.86058957591999E-5 2.8173113074057112E-5 1.1269245229622845E-4 0.0011691841925733702 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 63735 0.0 94.04969 1 TCGACGG 65065 0.0 92.18838 2 ATATCTC 39155 0.0 90.24894 1 TATCTCG 39665 0.0 88.99944 2 CGACGGC 67885 0.0 88.31681 3 ACGGCGC 68515 0.0 87.47006 5 GACGGCG 68775 0.0 87.16011 4 CGGCGCG 68935 0.0 86.895805 6 ATCTCGA 43035 0.0 82.019005 3 TCTCGAG 44310 0.0 78.640564 4 TCGAGGG 45705 0.0 76.157166 6 CTCGAGG 46235 0.0 75.3458 5 AGGGCGC 47460 0.0 73.90146 9 CGAGGGC 47235 0.0 73.74062 7 GAGGGCG 48520 0.0 72.22822 8 CGCGCCG 115065 0.0 53.35102 9 GGCGCGC 116200 0.0 52.948452 7 GCGCGCC 116035 0.0 52.905033 8 CCATATT 72070 0.0 41.768845 18-19 ATCCATA 72255 0.0 41.69806 16-17 >>END_MODULE