FastQCFastQC Report
Tue 31 May 2016
SRR1567935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8460082
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175630.20759846062957782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG42750.083.776041
TCGACGG49450.072.328712
CGACGGC77200.046.3297233
ACGGCGC84400.042.715095
GACGGCG89400.040.219844
CGGCGCG91000.039.4611856
CGCGCCG121500.032.721939
GGCGCGC129100.030.8692047
GCGCGCC130850.030.5289578
TATCTCG20700.030.0595862
ATCTCGA35400.023.8836841
GGGCGCG40150.020.5851218
TCTCGAG40800.020.489652
ATATCTC33300.019.5416361
TCGAGGG44400.018.9353054
CGCCGGA127650.018.0658410-11
CCATATT118100.018.0584218-19
ATCCATA119300.017.93655216-17
GCCGGAT125350.017.3362512-13
CTCGAGG48700.017.1658673
Produced by FastQC (version 0.11.5)