FastQCFastQC Report
Tue 31 May 2016
SRR1567935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8460082
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1308661.5468644393754103No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC272490.3220890766779802No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC228730.2703638097124827No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT153540.1814876026024334No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT113170.13376938899646598No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCA93900.11099183199406341No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA93780.1108499893972659No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCG93470.11048356268887227No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG90840.10737484577572652No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA84680.10009359247345356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1000950.093.5218351
TCGACGG1021950.091.587222
ATATCTC495550.089.844851
CGACGGC1050950.088.983123
TATCTCG500300.088.666172
ACGGCGC1058900.088.288155
GACGGCG1063400.087.9547354
CGGCGCG1068150.087.461336
ATCTCGA545200.081.337933
TCTCGAG554450.078.378944
TCGAGGG575200.075.411076
CTCGAGG578750.075.0475
CGAGGGC590350.073.4999547
AGGGCGC599300.073.060149
GAGGGCG607500.071.941068
CGCGCCG1653150.057.6779449
GGCGCGC1660900.057.5203487
GCGCGCC1661200.057.4413268
TGTCGAC19400.050.0081141
CCATATT1095000.042.8253718-19
Produced by FastQC (version 0.11.5)