FastQCFastQC Report
Tue 31 May 2016
SRR1567934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7223526
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168190.2328364291898444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG42300.084.330631
TCGACGG49200.072.503272
CGACGGC69650.051.2837223
ACGGCGC78650.045.536375
GACGGCG79700.044.7573554
CGGCGCG89950.039.711066
CGCGCCG115900.033.893779
GCGCGCC125000.031.6540878
GGCGCGC125200.031.5655847
ATCTCGA30100.029.3516311
TATCTCG15350.025.68342
TCTCGAG34800.024.9778252
GGGCGCG36500.024.075418
TCGAGGG40200.021.6225974
AGGGCGC43200.020.4514477
CTCGAGG43400.020.2471923
CGCCGGA119100.019.1238210-11
CCATATT112350.018.56009718-19
GCCGGAT114800.018.49532512-13
CGAGGGC48350.018.272685
Produced by FastQC (version 0.11.5)