##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567934_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7223526 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12313515587817 34.0 31.0 34.0 30.0 34.0 2 32.278457085916216 34.0 31.0 34.0 30.0 34.0 3 32.25117567237939 34.0 31.0 34.0 30.0 34.0 4 35.63926647457211 37.0 35.0 37.0 33.0 37.0 5 35.43140109137837 37.0 35.0 37.0 33.0 37.0 6 35.54417247200328 37.0 35.0 37.0 33.0 37.0 7 35.42591789660617 37.0 35.0 37.0 32.0 37.0 8 35.35388174694741 37.0 35.0 37.0 32.0 37.0 9 36.839833067673595 39.0 37.0 39.0 32.0 39.0 10-11 36.85750504670434 39.0 37.0 39.0 32.0 39.0 12-13 36.772395987776605 39.0 37.0 39.0 32.0 39.0 14-15 38.09623444284689 40.5 38.0 41.0 32.0 41.0 16-17 38.10581293678461 41.0 38.0 41.0 32.0 41.0 18-19 38.21855669931831 41.0 38.0 41.0 32.0 41.0 20-21 38.29725953779359 41.0 38.0 41.0 32.5 41.0 22-23 38.3664401567877 41.0 38.0 41.0 33.0 41.0 24-25 38.426424712806465 40.5 38.0 41.0 33.0 41.0 26-27 38.438221237107754 40.0 38.0 41.0 33.0 41.0 28-29 38.38435010824354 40.0 38.0 41.0 33.0 41.0 30-31 38.34477407570762 40.0 38.0 41.0 33.0 41.0 32-33 38.33069237931725 40.0 38.0 41.0 33.0 41.0 34-35 38.24611256331049 40.0 38.0 41.0 33.0 41.0 36-37 38.181931995537916 40.0 38.0 41.0 33.0 41.0 38-39 38.148759483941774 40.0 38.0 41.0 33.0 41.0 40-41 38.03427398198608 40.0 38.0 41.0 33.0 41.0 42-43 37.90335419295231 40.0 37.0 41.0 32.0 41.0 44-45 37.806951757355066 40.0 37.0 41.0 32.0 41.0 46-47 37.71221713606347 40.0 37.0 41.0 32.0 41.0 48-49 37.72908521406305 40.0 37.0 41.0 32.0 41.0 50-51 37.94541329262191 40.0 37.0 41.0 33.0 41.0 52-53 37.85578801820607 40.0 36.5 41.0 32.5 41.0 54-55 37.652022987111835 40.0 36.0 41.0 32.0 41.0 56-57 37.39098239557801 39.0 35.0 41.0 32.0 41.0 58-59 37.11389389337008 39.0 35.0 41.0 32.0 41.0 60-61 36.816478545242305 38.5 35.0 40.5 31.0 41.0 62-63 36.46951904097805 37.5 35.0 40.0 31.0 41.0 64-65 36.13867258178347 37.0 35.0 40.0 31.0 41.0 66-67 35.78002813307518 36.5 35.0 39.0 31.0 41.0 68-69 35.39238129689019 36.0 34.5 39.0 30.0 41.0 70-71 35.027582651464115 35.5 34.0 38.5 30.0 40.0 72-73 34.66877733948767 35.0 34.0 37.0 30.0 39.5 74-75 34.32185459012676 35.0 34.0 37.0 30.0 39.0 76-77 34.0328682280648 35.0 34.0 36.0 30.0 39.0 78-79 33.738124774521474 35.0 34.0 36.0 29.5 37.5 80-81 33.51969426011618 35.0 34.0 35.5 29.0 37.0 82-83 33.32414045439859 35.0 34.0 35.0 29.0 37.0 84-85 33.178473435272466 35.0 33.5 35.0 29.0 36.0 86-87 33.054405632374 35.0 33.0 35.0 29.0 36.0 88-89 32.92952458397741 35.0 33.0 35.0 29.0 36.0 90-91 32.81583384624074 35.0 33.0 35.0 29.0 35.5 92-93 32.72002924887375 35.0 33.0 35.0 29.0 35.0 94-95 32.62955390483816 35.0 33.0 35.0 29.0 35.0 96-97 32.53065615047277 35.0 33.0 35.0 29.0 35.0 98-99 32.41862478241236 35.0 33.0 35.0 29.0 35.0 100-101 31.976077195541347 34.5 32.5 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35999764834441805 1101 2 0.40406482715096104 1101 3 0.39150647349148926 1101 4 0.3660640105947266 1101 5 0.4359744307827995 1101 6 0.40714015426318184 1101 7 0.4311525460105514 1101 8 0.45120556002962786 1101 9 0.5973885975336799 1101 10-11 0.5969816116899196 1101 12-13 0.5749572158245329 1101 14-15 0.6658304356409062 1101 16-17 0.6618773178706405 1101 18-19 0.6719139797927483 1101 20-21 0.5654836677516926 1101 22-23 0.5274563197518916 1101 24-25 0.5374001378742292 1101 26-27 0.49813517946363106 1101 28-29 0.5271191926659782 1101 30-31 0.5151043170090759 1101 32-33 0.4530599250518321 1101 34-35 0.46597372427715555 1101 36-37 0.47162929819693744 1101 38-39 0.5301582990897415 1101 40-41 0.4807820268557421 1101 42-43 0.5048642457999648 1101 44-45 0.4613886340339093 1101 46-47 0.4710337777779543 1101 48-49 0.48765200669237885 1101 50-51 0.4579222608114719 1101 52-53 0.3855255777829427 1101 54-55 0.3852946555385728 1101 56-57 0.4226076706911783 1101 58-59 0.3922204067066133 1101 60-61 0.3684717739990546 1101 62-63 0.44300041845935567 1101 64-65 0.37938114528022737 1101 66-67 0.40363766976787474 1101 68-69 0.44806708591197975 1101 70-71 0.43286398016254424 1101 72-73 0.4227537159307886 1101 74-75 0.44030959634262956 1101 76-77 0.4031049911107303 1101 78-79 0.428667525254383 1101 80-81 0.44615553184151935 1101 82-83 0.45688400758798764 1101 84-85 0.4139543312323255 1101 86-87 0.36586559297361987 1101 88-89 0.36619830970894185 1101 90-91 0.38644123364481686 1101 92-93 0.3627541346462664 1101 94-95 0.38354099796031704 1101 96-97 0.41747872090405735 1101 98-99 0.4336636220020509 1101 100-101 0.43260315962766427 1102 1 0.3598925357364138 1102 2 0.32568355737964083 1102 3 0.3828673896217367 1102 4 0.39158552100565913 1102 5 0.3904687964479834 1102 6 0.3694048657002469 1102 7 0.3938614924843691 1102 8 0.44605748168404347 1102 9 0.5195313956576655 1102 10-11 0.5548960225242041 1102 12-13 0.6120663830289246 1102 14-15 0.6701515415758408 1102 16-17 0.6720056248946733 1102 18-19 0.6208803724806984 1102 20-21 0.6105167675014513 1102 22-23 0.582341616577466 1102 24-25 0.5283481284784273 1102 26-27 0.4986418171192497 1102 28-29 0.4852959128693186 1102 30-31 0.534916735366302 1102 32-33 0.49770525115076225 1102 34-35 0.4760140931844461 1102 36-37 0.4554891716604743 1102 38-39 0.42015898764322657 1102 40-41 0.4517864985188069 1102 42-43 0.49358498227677217 1102 44-45 0.4280230337726536 1102 46-47 0.39262300840795916 1102 48-49 0.4491222651163369 1102 50-51 0.4055464070051471 1102 52-53 0.38649529716789033 1102 54-55 0.4389331787290516 1102 56-57 0.43393404110934597 1102 58-59 0.3690421421761627 1102 60-61 0.37557346547987436 1102 62-63 0.4103133384769819 1102 64-65 0.40506772163490723 1102 66-67 0.3838983364450357 1102 68-69 0.37329329540536094 1102 70-71 0.3829022145540151 1102 72-73 0.37731614018989745 1102 74-75 0.353122396355225 1102 76-77 0.3131475240432664 1102 78-79 0.343111606842065 1102 80-81 0.3429672439395972 1102 82-83 0.36251835224244644 1102 84-85 0.33791375969358484 1102 86-87 0.3232678085420062 1102 88-89 0.33064378807655714 1102 90-91 0.36667272532709205 1102 92-93 0.395038528876583 1102 94-95 0.4496282632619355 1102 96-97 0.41608180326292654 1102 98-99 0.39626317699976 1102 100-101 0.4371213885894072 1103 1 0.27310947669391794 1103 2 0.31906065586471755 1103 3 0.330036984642355 1103 4 0.27819311483268194 1103 5 0.3296680762448645 1103 6 0.30625795572900927 1103 7 0.29758206986068814 1103 8 0.3237383332500485 1103 9 0.4512048226578642 1103 10-11 0.4381124914384742 1103 12-13 0.4254527200745457 1103 14-15 0.5465951340946233 1103 16-17 0.5718622923368386 1103 18-19 0.5117543605380064 1103 20-21 0.4498478822315519 1103 22-23 0.4331563294908847 1103 24-25 0.46117833115356177 1103 26-27 0.4752359914313331 1103 28-29 0.45242349604165355 1103 30-31 0.39664174994906887 1103 32-33 0.4546551774782941 1103 34-35 0.40409658611222454 1103 36-37 0.39166464361942843 1103 38-39 0.370747794149338 1103 40-41 0.3817448912790198 1103 42-43 0.40981278360051476 1103 44-45 0.367866484004125 1103 46-47 0.3443425607760986 1103 48-49 0.38651810371801076 1103 50-51 0.38661865322289657 1103 52-53 0.39410857911594377 1103 54-55 0.4159983847590709 1103 56-57 0.4166303858088085 1103 58-59 0.39398831238821685 1103 60-61 0.3985847304546084 1103 62-63 0.41470920742429485 1103 64-65 0.38296082407165954 1103 66-67 0.3754906057254743 1103 68-69 0.39122035123796195 1103 70-71 0.4068309629988036 1103 72-73 0.40364539709269565 1103 74-75 0.4099933228749535 1103 76-77 0.40100788690964606 1103 78-79 0.40251285371056866 1103 80-81 0.3977621242935001 1103 82-83 0.3575623365571232 1103 84-85 0.3363548031788781 1103 86-87 0.35608934585197005 1103 88-89 0.36888556188018384 1103 90-91 0.36343713866163796 1103 92-93 0.3923570535391576 1103 94-95 0.41408408170062216 1103 96-97 0.3937967430942706 1103 98-99 0.3809478352573237 1103 100-101 0.3937868751479776 1104 1 0.21034000249304796 1104 2 0.21680935755060915 1104 3 0.2270536954407234 1104 4 0.23991728663764178 1104 5 0.23712822023911428 1104 6 0.27216049393869923 1104 7 0.25468119539305434 1104 8 0.3045682702330552 1104 9 0.4072563417411317 1104 10-11 0.4084059676594265 1104 12-13 0.43689671390445284 1104 14-15 0.5201295079860486 1104 16-17 0.4941663557389333 1104 18-19 0.5231669006369088 1104 20-21 0.48927423314305685 1104 22-23 0.49921434305312573 1104 24-25 0.4838593087145284 1104 26-27 0.4444647936170725 1104 28-29 0.46107244919281243 1104 30-31 0.44145120049423525 1104 32-33 0.43513902410757765 1104 34-35 0.4629930802682978 1104 36-37 0.41577264446681284 1104 38-39 0.4174125750108928 1104 40-41 0.4165290857692483 1104 42-43 0.41166077896924946 1104 44-45 0.4057875107083788 1104 46-47 0.4114796061434163 1104 48-49 0.41551215863653823 1104 50-51 0.3750292656918788 1104 52-53 0.34469688164221424 1104 54-55 0.34910862785881847 1104 56-57 0.3383532149069097 1104 58-59 0.3473825516082911 1104 60-61 0.35338286730413415 1104 62-63 0.35459509777145115 1104 64-65 0.3673774937035219 1104 66-67 0.32418000806259073 1104 68-69 0.33007161671647367 1104 70-71 0.3494569883237588 1104 72-73 0.2979225404140564 1104 74-75 0.31050741332356324 1104 76-77 0.3324967802666592 1104 78-79 0.3322254696105418 1104 80-81 0.28504489137738176 1104 82-83 0.3023503270601111 1104 84-85 0.2817436493254988 1104 86-87 0.3093112067086068 1104 88-89 0.31449235670327624 1104 90-91 0.3537315841739286 1104 92-93 0.35427150835732135 1104 94-95 0.3439659547097236 1104 96-97 0.28533362375015514 1104 98-99 0.35040545375957066 1104 100-101 0.3983792759379092 1105 1 0.12771194845097966 1105 2 0.1619073540625493 1105 3 0.15934172969113547 1105 4 0.11913537827410181 1105 5 0.11377052611267402 1105 6 0.13860170730890786 1105 7 0.20554938426708702 1105 8 0.20924890189242262 1105 9 0.19680591672262437 1105 10-11 0.2551847170278805 1105 12-13 0.24969383602911677 1105 14-15 0.2913252516884981 1105 16-17 0.2967672897716085 1105 18-19 0.24699657369997396 1105 20-21 0.2993549763791634 1105 22-23 0.344957679745292 1105 24-25 0.3204252171591975 1105 26-27 0.3230977086235072 1105 28-29 0.2771680927510545 1105 30-31 0.2968553963095957 1105 32-33 0.3166202937726794 1105 34-35 0.3376296620010919 1105 36-37 0.3539027747373993 1105 38-39 0.36824065577507525 1105 40-41 0.31343827585475736 1105 42-43 0.30473453926568084 1105 44-45 0.31813825381537697 1105 46-47 0.26772507475826046 1105 48-49 0.2517022561700415 1105 50-51 0.17815594153893954 1105 52-53 0.21417849755320617 1105 54-55 0.14790096782218853 1105 56-57 0.22243653802964758 1105 58-59 0.1761580381300547 1105 60-61 0.21998301685265176 1105 62-63 0.21626019031603505 1105 64-65 0.22857221994842547 1105 66-67 0.20574829639365078 1105 68-69 0.23603040170591782 1105 70-71 0.25933628217053695 1105 72-73 0.26173877705250703 1105 74-75 0.2741077450280258 1105 76-77 0.2621735064074855 1105 78-79 0.2714919270555711 1105 80-81 0.2260265627885829 1105 82-83 0.25186298650959316 1105 84-85 0.20568353033062436 1105 86-87 0.19543020690250756 1105 88-89 0.2275336935759853 1105 90-91 0.2239251017169508 1105 92-93 0.24138395664557066 1105 94-95 0.2575625291363295 1105 96-97 0.3047352395680534 1105 98-99 0.3253176163062861 1105 100-101 0.33808650696323284 1106 1 0.13445118808726164 1106 2 0.15105618162652235 1106 3 0.19318148723132111 1106 4 0.14522804756845886 1106 5 0.16731943944946437 1106 6 0.11997383587354449 1106 7 0.17523856792180936 1106 8 0.14326133317445056 1106 9 0.249183495073801 1106 10-11 0.2271820999823717 1106 12-13 0.21940120940455898 1106 14-15 0.3939168018028667 1106 16-17 0.3931349049751276 1106 18-19 0.36607574063513937 1106 20-21 0.3326086873800165 1106 22-23 0.3592943203498038 1106 24-25 0.3301334469992909 1106 26-27 0.29298768766223304 1106 28-29 0.28724633517369114 1106 30-31 0.3342852343466305 1106 32-33 0.26395321412579875 1106 34-35 0.314587058254709 1106 36-37 0.24338058309761124 1106 38-39 0.23311511827356668 1106 40-41 0.23665850351010675 1106 42-43 0.28882844750067704 1106 44-45 0.22420236646655667 1106 46-47 0.26352856494646204 1106 48-49 0.24766007695576064 1106 50-51 0.20626832865302447 1106 52-53 0.23677424154662674 1106 54-55 0.22148874877012048 1106 56-57 0.20654380765464708 1106 58-59 0.2226719111426121 1106 60-61 0.2564950388546805 1106 62-63 0.24030844961706066 1106 64-65 0.23260066323863526 1106 66-67 0.18414454874261565 1106 68-69 0.2192249724239943 1106 70-71 0.2288518173923535 1106 72-73 0.21500892423266293 1106 74-75 0.22950291727488548 1106 76-77 0.23291234660093352 1106 78-79 0.2569873553032451 1106 80-81 0.21542755105311784 1106 82-83 0.1904120451475464 1106 84-85 0.12048110955901592 1106 86-87 0.20448797485636305 1106 88-89 0.1813237120897071 1106 90-91 0.21739529882295017 1106 92-93 0.20333636753488804 1106 94-95 0.19779075034137605 1106 96-97 0.2741127567302897 1106 98-99 0.27002229123147004 1106 100-101 0.21833098565422304 1107 1 0.06896919915560318 1107 2 0.012281615294575943 1107 3 0.09828194656928702 1107 4 0.12775047474048762 1107 5 0.15798612015779412 1107 6 0.08229645015076414 1107 7 0.10625186713389212 1107 8 0.0872892374734775 1107 9 0.14434535159255546 1107 10-11 0.09129401731908615 1107 12-13 0.1511917586618452 1107 14-15 0.13086726807390647 1107 16-17 0.102592229721985 1107 18-19 0.15307759062451964 1107 20-21 0.1408733043146171 1107 22-23 0.19889901237220897 1107 24-25 0.1685979558130697 1107 26-27 0.11290973886868017 1107 28-29 0.1667644603305689 1107 30-31 0.22282297922010486 1107 32-33 0.1703489293116789 1107 34-35 0.16844531291083342 1107 36-37 0.234641533402403 1107 38-39 0.19982408545644148 1107 40-41 0.14022043515176819 1107 42-43 0.1629808136090105 1107 44-45 0.1592840626294958 1107 46-47 0.1240857469948935 1107 48-49 0.2327489694720768 1107 50-51 0.1846454368774033 1107 52-53 0.14464665722204728 1107 54-55 0.12004706468301407 1107 56-57 0.04511411163914403 1107 58-59 0.09889138587902835 1107 60-61 5.779250487449872E-4 1107 62-63 0.06128954953580035 1107 64-65 0.07726009142692902 1107 66-67 0.06916858631811351 1107 68-69 0.06577638334805869 1107 70-71 0.07491944907246051 1107 72-73 0.06825232947686999 1107 74-75 0.13725913817134483 1107 76-77 0.11601710795405751 1107 78-79 0.13661733109892538 1107 80-81 0.14037296407992272 1107 82-83 0.1428505558183346 1107 84-85 0.07828511599712584 1107 86-87 0.11033606510214611 1107 88-89 0.13604315962039237 1107 90-91 0.10640730093622608 1107 92-93 0.06398661949501872 1107 94-95 0.03890654493287826 1107 96-97 0.016997318446037468 1107 98-99 0.043675300623590374 1107 100-101 0.044114861610157874 1108 1 0.03612086594552011 1108 2 0.017913347983707695 1108 3 0.09701423923738872 1108 4 0.01647350289966454 1108 5 0.025010733759984305 1108 6 -0.049187833051512087 1108 7 0.01609186434016152 1108 8 -0.004321349128112217 1108 9 -0.0573414155663059 1108 10-11 -0.005047913930447123 1108 12-13 -0.010861963164593647 1108 14-15 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64-65 -0.28883074016419386 2214 66-67 -0.356663745763349 2214 68-69 -0.3243588514306097 2214 70-71 -0.2913021018256572 2214 72-73 -0.27465461335316377 2214 74-75 -0.24111745306581156 2214 76-77 -0.25713487117403844 2214 78-79 -0.24254398075354544 2214 80-81 -0.23258037252307417 2214 82-83 -0.19045741171794361 2214 84-85 -0.23240293574550464 2214 86-87 -0.22914938475733493 2214 88-89 -0.2038502568084155 2214 90-91 -0.23354975485931107 2214 92-93 -0.1739710358148301 2214 94-95 -0.21220996615465282 2214 96-97 -0.23740454683954226 2214 98-99 -0.2985460123577397 2214 100-101 -0.23765892495240237 2215 1 -0.41593191287093845 2215 2 -0.41503169197087075 2215 3 -0.46869138833334745 2215 4 -0.46476437916162894 2215 5 -0.4861826062209076 2215 6 -0.4796958689760089 2215 7 -0.5311121809740058 2215 8 -0.5193088157172241 2215 9 -0.7330523057847032 2215 10-11 -0.7021409502704117 2215 12-13 -0.6915594717163955 2215 14-15 -0.782891390345192 2215 16-17 -0.7714924516874291 2215 18-19 -0.7632373992839803 2215 20-21 -0.7071895855594477 2215 22-23 -0.6886841934551455 2215 24-25 -0.6813923450138688 2215 26-27 -0.6079088277461153 2215 28-29 -0.6539539541488821 2215 30-31 -0.6360638608546978 2215 32-33 -0.5988998969793968 2215 34-35 -0.5660869036008691 2215 36-37 -0.6781118983611876 2215 38-39 -0.6655063838963571 2215 40-41 -0.6084916454407292 2215 42-43 -0.47622749411414134 2215 44-45 -0.5090791964670203 2215 46-47 -0.5150515061121155 2215 48-49 -0.5337845242790351 2215 50-51 -0.5351853397975077 2215 52-53 -0.4736783500795809 2215 54-55 -0.438825172142451 2215 56-57 -0.4695805714703454 2215 58-59 -0.5556703858656533 2215 60-61 -0.5457679932340938 2215 62-63 -0.5254764192263224 2215 64-65 -0.4837301614490386 2215 66-67 -0.5229308378343518 2215 68-69 -0.4419626849210445 2215 70-71 -0.3958325310845652 2215 72-73 -0.41064619319740103 2215 74-75 -0.377382471934979 2215 76-77 -0.4048731671537311 2215 78-79 -0.3652111264928024 2215 80-81 -0.3328503435778387 2215 82-83 -0.4044079565682708 2215 84-85 -0.342448991526382 2215 86-87 -0.36046975091434774 2215 88-89 -0.3396269551567883 2215 90-91 -0.350587001616951 2215 92-93 -0.3508551859025175 2215 94-95 -0.33887112386542384 2215 96-97 -0.35727600643048874 2215 98-99 -0.29242338682764313 2215 100-101 -0.23516824962301186 2216 1 -0.5603190235984634 2216 2 -0.5283190359099201 2216 3 -0.4846616849792227 2216 4 -0.505703713560024 2216 5 -0.5166124254666684 2216 6 -0.5192134904224872 2216 7 -0.5149896745316127 2216 8 -0.5278868375203558 2216 9 -0.6660961140493242 2216 10-11 -0.6526402738957842 2216 12-13 -0.7292372623483558 2216 14-15 -0.9326545061980624 2216 16-17 -0.8905691748104587 2216 18-19 -0.9096281805936002 2216 20-21 -0.8256680282859179 2216 22-23 -0.8893827568476667 2216 24-25 -0.8705315333235006 2216 26-27 -0.8899350135440898 2216 28-29 -0.9646893865899742 2216 30-31 -0.8583327049784018 2216 32-33 -0.8685254731220411 2216 34-35 -0.8523856245080808 2216 36-37 -0.7615607152666755 2216 38-39 -0.8935053861672557 2216 40-41 -0.789791162082814 2216 42-43 -0.8475659915626892 2216 44-45 -0.8056511094748373 2216 46-47 -0.9267922169277796 2216 48-49 -0.7812086897266042 2216 50-51 -0.7356570677459615 2216 52-53 -0.6592593793524273 2216 54-55 -0.6592773943850787 2216 56-57 -0.603516877995574 2216 58-59 -0.5851683281769127 2216 60-61 -0.5609719359983103 2216 62-63 -0.4864126727025635 2216 64-65 -0.5426206112024587 2216 66-67 -0.5053741723306899 2216 68-69 -0.5364856826786948 2216 70-71 -0.4685163305348894 2216 72-73 -0.5076147494283063 2216 74-75 -0.46538401443639543 2216 76-77 -0.5016372807357001 2216 78-79 -0.4539699433370856 2216 80-81 -0.46871095123362494 2216 82-83 -0.4664314083028671 2216 84-85 -0.508693255604264 2216 86-87 -0.5451604752983172 2216 88-89 -0.5370506105179018 2216 90-91 -0.6020711491502766 2216 92-93 -0.5978928588473309 2216 94-95 -0.5778304556929825 2216 96-97 -0.6276667632559558 2216 98-99 -0.5960898372176544 2216 100-101 -0.6511012589494314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 74.0 11 962.0 12 3533.0 13 6804.0 14 9116.0 15 10474.0 16 11053.0 17 11622.0 18 13277.0 19 17373.0 20 18239.0 21 23247.0 22 32209.0 23 41335.0 24 48717.0 25 54013.0 26 58822.0 27 66268.0 28 82844.0 29 103187.0 30 110066.0 31 127128.0 32 173400.0 33 236719.0 34 322707.0 35 471124.0 36 831333.0 37 1852658.0 38 2125982.0 39 359046.0 40 194.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.279084634979903 26.473254784848134 25.54899100558055 25.69866957459141 2 25.49131822879851 26.695190132907392 25.955911282107934 21.857580356186162 3 24.334500907174693 25.435583674787075 25.183878344177067 25.04603707386116 4 25.39899489529075 24.473726542965306 24.9629336144149 25.16434494732905 5 25.66426737538835 25.49360270516238 25.024814764875085 23.81731515457418 6 25.55318552186287 24.815830939073244 24.04832487624465 25.582658662819235 7 26.24566398888519 25.265670448342547 24.27224854596452 24.21641701680774 8 25.3346813525637 25.249168055432886 24.642517870299432 24.773632721703986 9 24.43314308433732 24.929494168767675 24.935446948549707 25.701915798345293 10-11 25.09881283393588 25.21136207422851 24.54420672512859 25.145618366707023 12-13 24.93631129648355 25.369029002344597 24.57491026406343 25.119749437108425 14-15 24.841021191129386 25.60989926277579 24.68709883245492 24.861980713639902 16-17 24.715839538241454 25.804160038142093 24.807955350826063 24.67204507279039 18-19 24.385269433199657 25.463940838955573 25.100950450472588 25.049839277372183 20-21 24.29384901501012 25.72666728132494 25.08230744929831 24.897176254366634 22-23 24.355176405539343 25.58022910141114 25.57215825069364 24.49243624235588 24-25 24.330203269452657 25.16644593854605 25.953079290529313 24.55027150147198 26-27 24.348991491687748 25.20531177175277 26.04592629396513 24.39977044259435 28-29 24.33960174540944 25.292045983121803 26.02777454758899 24.34057772387977 30-31 24.34046650941091 25.225628876509194 26.022738080290143 24.411166533789753 32-33 24.35262992826238 25.260406121623014 26.05168024011707 24.335283709997533 34-35 24.381012080904284 25.321272479555923 25.99592478609003 24.30179065344976 36-37 24.420490653092912 25.258252678257957 25.8905906842142 24.430665984434928 38-39 24.4616483711895 25.309017597007955 25.814443319784107 24.414890712018437 40-41 24.589348747412274 25.477232310093434 25.46496683198759 24.468452110506696 42-43 24.58029840001938 25.458574587891647 25.42500372048273 24.536123291606245 44-45 24.55539718414525 25.596633832286336 25.398987973463377 24.448981010105037 46-47 24.56417017799773 25.682233271622874 25.292347123334206 24.461249427045185 48-49 24.60664762230419 25.60501613833212 25.28545432017925 24.50288191918444 50-51 24.533067156012077 25.664717637685502 25.32516157881731 24.477053627485116 52-53 24.545856317485697 25.70233168373105 25.320451270043304 24.43136072873995 54-55 24.530914828835062 25.584252436622005 25.373847972563933 24.510984761979 56-57 24.526756610892274 25.618779267849767 25.4356056948479 24.418858426410054 58-59 24.562192749612482 25.673473605998083 25.410579170030516 24.353754474358915 60-61 24.506824752051372 25.59002204256995 25.389655808506166 24.51349739687251 62-63 24.53604375480894 25.640615123417565 25.395492450639757 24.427848671133738 64-65 24.527924451299825 25.716914426555675 25.347254235673827 24.40790688647068 66-67 24.514703761016435 25.674808950642664 25.29663491209141 24.513852376249492 68-69 24.504736329598593 25.75654881009634 25.31360031098386 24.425114549321204 70-71 24.579374394167058 25.773569583607784 25.257028215860235 24.390027806364927 72-73 24.51728560262675 25.74314123047387 25.25065321284924 24.488919954050143 74-75 24.52980026651804 25.79554638551865 25.276236286821703 24.398417061141608 76-77 24.539158577127015 25.833768716275124 25.24461045755217 24.382462249045687 78-79 24.581928548467882 25.777459650591695 25.22935475002097 24.411257050919456 80-81 24.47414877443509 25.835208456368814 25.299445174005047 24.39119759519105 82-83 24.54652497496547 25.856965786849035 25.262968965214828 24.333540272970662 84-85 24.451683755973367 25.84358075692429 25.255065983731207 24.449669503371137 86-87 24.48418586219368 25.907193498872584 25.274088071864035 24.3345325670697 88-89 24.507723531089322 25.962319578922887 25.215307792197006 24.314649097790785 90-91 24.504410839912936 25.91153262713287 25.216395747292047 24.36766078566215 92-93 24.47373588872358 25.974480938230077 25.211025832836043 24.340757340210295 94-95 24.50348852252313 26.046665776513194 25.201649871076565 24.248195829887102 96-97 24.428026472687385 26.06083141869674 25.159733401462624 24.35140870715325 98-99 24.39769029039986 26.100388516351238 25.21907818856094 24.282843004687958 100-101 24.452268726455397 26.15595933508354 25.150861269374893 24.24091066908617 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14467.0 1 8196.5 2 1598.5 3 1051.0 4 745.0 5 569.5 6 424.0 7 355.0 8 311.5 9 280.5 10 295.5 11 328.0 12 347.5 13 387.5 14 497.0 15 670.5 16 1690.5 17 1891.0 18 1202.0 19 1540.0 20 2221.0 21 2986.0 22 4048.0 23 5754.0 24 7601.5 25 9833.0 26 12785.0 27 16705.0 28 21941.5 29 28129.0 30 35689.5 31 43357.5 32 51135.0 33 60625.0 34 71180.0 35 82759.5 36 97020.5 37 114078.0 38 131504.0 39 149196.5 40 164966.0 41 177425.0 42 191643.5 43 207934.0 44 225608.0 45 247675.5 46 271760.5 47 293222.0 48 309094.0 49 319168.0 50 321418.0 51 319564.0 52 323057.0 53 326504.0 54 318686.0 55 303907.5 56 285253.5 57 262939.5 58 241934.0 59 219207.5 60 190928.0 61 162517.5 62 135075.0 63 105753.5 64 79559.0 65 58023.0 66 41627.5 67 31118.0 68 23720.0 69 18668.0 70 15111.5 71 12076.5 72 10177.5 73 8170.5 74 6157.5 75 4842.0 76 4127.0 77 3225.0 78 1834.0 79 843.5 80 391.0 81 206.0 82 109.5 83 38.0 84 16.0 85 6.5 86 5.0 87 3.0 88 1.5 89 1.0 90 3.5 91 3.5 92 2.5 93 3.5 94 2.5 95 1.5 96 1.5 97 3.0 98 5.0 99 6.0 100 16.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.506517731091437E-4 2 0.0 3 0.0 4 0.0 5 5.537461898801222E-5 6 0.0 7 1.245928927230275E-4 8 0.001218241617736269 9 1.3843654747003057E-4 10-11 2.907167496870642E-4 12-13 0.0015228020221703363 14-15 0.0010936487250132415 16-17 1.453583748435321E-4 18-19 7.821664932056726E-4 20-21 0.0 22-23 0.0 24-25 0.0011697888261217582 26-27 8.375411121936848E-4 28-29 8.306192848201834E-5 30-31 6.783390826031497E-4 32-33 6.852609099766512E-4 34-35 0.0014051309568208102 36-37 0.0024087959259785317 38-39 0.0015297238495438378 40-41 0.0 42-43 1.3843654747003056E-5 44-45 0.0 46-47 9.690558322902139E-5 48-49 5.675898446271253E-4 50-51 0.0037516304364378283 52-53 0.013525250687821986 54-55 0.010666535982565855 56-57 6.852609099766512E-4 58-59 0.00393851977552237 60-61 4.8452791614510694E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.460913686750764E-5 84-85 4.153096424100917E-5 86-87 0.0022634375511349997 88-89 0.002021173593062446 90-91 0.0017789096349898927 92-93 0.0024987796818340515 94-95 7.267918742176605E-4 96-97 7.614010110851681E-5 98-99 2.8379492231356263E-4 100-101 2.768730949400611E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7223526.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.67090789033755 #Duplication Level Percentage of deduplicated Percentage of total 1 79.40255187298774 44.99814703451839 2 10.763982107991582 12.200092771504648 3 3.5162164751401783 5.978015399554693 4 1.659300031901596 3.7613615708131807 5 0.9395084197437855 2.662139755874833 6 0.6067828386759179 2.0632160616024295 7 0.436349781423923 1.7309836789730846 8 0.3328497208645921 1.509031669795349 9 0.25644696655336074 1.3079774178271784 >10 2.022958662521786 21.086046848015965 >50 0.05943614067239381 2.1024582478439218 >100 0.0034455189400262863 0.2656718479116693 >500 9.798278067989181E-5 0.038979741784588774 >1k 4.8986534933506435E-5 0.062420865033243525 >5k 0.0 0.0 >10k+ 2.4493267466753065E-5 0.23345708894687936 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16819 0.2328364291898444 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.3843654747003056E-5 0.0 0.0 0.0 0.0 3 2.768730949400611E-5 0.0 0.0 0.0 1.3843654747003056E-5 4 5.537461898801222E-5 0.0 0.0 0.0 1.3843654747003056E-5 5 6.921827373501529E-5 0.0 0.0 0.0 2.768730949400611E-5 6 8.306192848201834E-5 0.0 0.0 0.0 2.768730949400611E-5 7 1.1074923797602445E-4 0.0 0.0 0.0 2.768730949400611E-5 8 1.245928927230275E-4 0.0 0.0 0.0 2.768730949400611E-5 9 2.0765482120504585E-4 0.0 0.0 0.0 4.153096424100917E-5 10-11 5.122152256391131E-4 0.0 0.0 0.0 6.229644636151375E-5 12-13 9.067593859287002E-4 0.0 0.0 0.0 7.614010110851681E-5 14-15 0.001010586796531223 0.0 0.0 0.0 9.69055832290214E-5 16-17 0.0010451959333987308 0.0 0.0 0.0 1.3151472009652904E-4 18-19 0.001363599992579801 0.0 0.0 0.0 1.5228020221703362E-4 20-21 0.0017996751171103973 1.3843654747003056E-5 0.0 0.0 1.7304568433753822E-4 22-23 0.0022357502416409936 1.3843654747003056E-5 0.0 0.0 2.007329938315443E-4 24-25 0.00249185785446055 1.3843654747003056E-5 0.0 0.0 2.2842030332555043E-4 26-27 0.0026510598840510853 1.3843654747003056E-5 0.0 0.0 2.49185785446055E-4 28-29 0.0029417766337381497 2.0765482120504583E-5 0.0 0.0 2.630294401930581E-4 30-31 0.0031494314549431954 2.768730949400611E-5 0.0 0.0 2.9071674968706416E-4 32-33 0.0031701969370636998 2.768730949400611E-5 0.0 0.0 3.391695413015749E-4 34-35 0.0032186497286782103 2.768730949400611E-5 0.0 0.0 3.460913686750764E-4 36-37 0.0033363207940277366 2.768730949400611E-5 0.0 0.0 3.460913686750764E-4 38-39 0.0034124608951362533 2.768730949400611E-5 0.0 0.0 3.5993502342207946E-4 40-41 0.0035162883057387765 2.768730949400611E-5 0.0 1.3843654747003056E-5 3.5993502342207946E-4 42-43 0.0035924284068472928 2.768730949400611E-5 0.0 1.3843654747003056E-5 3.7377867816908253E-4 44-45 0.0036270375437148007 4.153096424100917E-5 0.0 1.3843654747003056E-5 3.8070050554258407E-4 46-47 0.003647803025835305 4.153096424100917E-5 0.0 1.3843654747003056E-5 3.876223329160856E-4 48-49 0.0038969888112813603 4.153096424100917E-5 0.0 1.3843654747003056E-5 4.2223146978359323E-4 50-51 0.005461321797692705 4.153096424100917E-5 0.0 1.3843654747003056E-5 4.429969519040978E-4 52-53 0.005745116720006269 4.153096424100917E-5 0.0 1.3843654747003056E-5 4.5684060665110085E-4 54-55 0.006042755297066834 5.537461898801223E-5 0.0 1.3843654747003056E-5 4.8452791614510694E-4 56-57 0.006381924838368409 6.921827373501529E-5 0.0 1.3843654747003056E-5 5.122152256391131E-4 58-59 0.006707250724922981 6.921827373501529E-5 0.0 2.0765482120504583E-5 5.329807077596177E-4 60-61 0.007177934986321084 6.921827373501529E-5 0.0 2.768730949400611E-5 5.606680172536238E-4 62-63 0.007780133967815718 6.921827373501529E-5 0.0 4.153096424100917E-5 5.814334993741283E-4 64-65 0.008728424317985428 6.921827373501529E-5 0.0 4.153096424100917E-5 6.091208088681345E-4 66-67 0.009773620251384157 6.921827373501529E-5 0.0 5.537461898801222E-5 6.229644636151375E-4 68-69 0.01119259486295197 6.921827373501529E-5 0.0 5.537461898801222E-5 6.506517731091437E-4 70-71 0.012826146123098332 6.921827373501529E-5 0.0 6.229644636151375E-5 6.852609099766514E-4 72-73 0.015214176566956358 6.921827373501529E-5 0.0 6.921827373501529E-5 7.129482194706574E-4 74-75 0.018197484164935517 7.614010110851681E-5 0.0 6.921827373501529E-5 7.198700468441589E-4 76-77 0.022115238458337384 8.306192848201834E-5 0.0 6.921827373501529E-5 7.267918742176605E-4 78-79 0.02712664147675249 8.998375585551988E-5 0.0 6.921827373501529E-5 7.406355289646635E-4 80-81 0.034228436361965056 9.69055832290214E-5 0.0 6.921827373501529E-5 8.029319753261772E-4 82-83 0.04255539469228739 9.69055832290214E-5 0.0 6.921827373501529E-5 8.167756300731803E-4 84-85 0.053062728645262715 9.69055832290214E-5 0.0 6.921827373501529E-5 8.51384766940688E-4 86-87 0.06664335395207271 9.69055832290214E-5 0.0 6.921827373501529E-5 8.859939038081956E-4 88-89 0.08349800360654894 9.69055832290214E-5 0.0 6.921827373501529E-5 8.998375585551986E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4230 0.0 84.33063 1 TCGACGG 4920 0.0 72.50327 2 CGACGGC 6965 0.0 51.283722 3 ACGGCGC 7865 0.0 45.53637 5 GACGGCG 7970 0.0 44.757355 4 CGGCGCG 8995 0.0 39.71106 6 CGCGCCG 11590 0.0 33.89377 9 GCGCGCC 12500 0.0 31.654087 8 GGCGCGC 12520 0.0 31.565584 7 ATCTCGA 3010 0.0 29.351631 1 TATCTCG 1535 0.0 25.6834 2 TCTCGAG 3480 0.0 24.977825 2 GGGCGCG 3650 0.0 24.07541 8 TCGAGGG 4020 0.0 21.622597 4 AGGGCGC 4320 0.0 20.451447 7 CTCGAGG 4340 0.0 20.247192 3 CGCCGGA 11910 0.0 19.12382 10-11 CCATATT 11235 0.0 18.560097 18-19 GCCGGAT 11480 0.0 18.495325 12-13 CGAGGGC 4835 0.0 18.27268 5 >>END_MODULE