FastQCFastQC Report
Tue 31 May 2016
SRR1567934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7223526
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1616412.237702196960321No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC193500.2678747193545091No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC189230.26196347877753884No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT142210.19687061415713047No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT105880.14657661646126835No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA99020.13707986930482427No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG97990.13565397286588296No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCG91700.12694631403001802No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCA89260.12356846227174927No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT88440.12243328258249504No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA85510.11837709174162313No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG81700.11310265928301498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG846300.093.606731
TCGACGG867950.091.398062
ATATCTC490550.090.544251
TATCTCG499300.088.691782
CGACGGC894050.088.65553
ACGGCGC904500.087.636485
GACGGCG907200.087.417554
CGGCGCG918050.086.301636
ATCTCGA535650.082.699623
TCTCGAG540200.080.490684
CTCGAGG561800.077.353715
TCGAGGG562500.077.291226
CGAGGGC572500.076.040727
AGGGCGC580250.075.8191459
GAGGGCG587150.074.7825248
CGCGCCG1483200.054.50989
GGCGCGC1495700.054.206687
GCGCGCC1493400.054.1883858
CTCGACG18650.042.7877735
TGTCGAC17500.042.6546021
Produced by FastQC (version 0.11.5)