##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567934_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7223526 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.870167145518685 34.0 31.0 34.0 31.0 34.0 2 33.056116362009355 34.0 33.0 34.0 31.0 34.0 3 33.14270980681734 34.0 33.0 34.0 31.0 34.0 4 36.552984511995945 37.0 37.0 37.0 35.0 37.0 5 36.40953573088821 37.0 37.0 37.0 35.0 37.0 6 36.425441674882876 37.0 37.0 37.0 35.0 37.0 7 36.391041023455855 37.0 37.0 37.0 35.0 37.0 8 36.38475863449512 37.0 37.0 37.0 35.0 37.0 9 38.18439166689509 39.0 39.0 39.0 37.0 39.0 10-11 38.19903368521135 39.0 39.0 39.0 37.0 39.0 12-13 38.18678011264859 39.0 39.0 39.0 37.0 39.0 14-15 39.75237577880941 41.0 40.0 41.0 37.5 41.0 16-17 39.66076864677998 41.0 39.5 41.0 37.5 41.0 18-19 39.7425128669849 41.0 40.0 41.0 37.5 41.0 20-21 39.795957195973266 41.0 40.0 41.0 38.0 41.0 22-23 39.787890706007005 41.0 40.0 41.0 37.5 41.0 24-25 39.778587562362205 41.0 40.0 41.0 37.0 41.0 26-27 39.75538656606206 41.0 40.0 41.0 37.0 41.0 28-29 39.55119743460465 41.0 39.5 41.0 37.0 41.0 30-31 38.94549400112909 40.5 38.0 41.0 35.5 41.0 32-33 38.5095142593797 40.0 36.0 41.0 35.0 41.0 34-35 38.37880676279147 40.0 35.0 41.0 35.0 41.0 36-37 38.300828916515286 40.0 35.0 41.0 35.0 41.0 38-39 37.98290225576817 40.0 35.0 41.0 34.0 41.0 40-41 37.056425698474676 40.0 35.0 41.0 30.5 41.0 42-43 37.287953279326466 40.0 35.0 41.0 31.0 41.0 44-45 37.22945504729961 40.0 35.0 41.0 31.0 41.0 46-47 37.06658763324171 40.0 35.0 41.0 31.0 41.0 48-49 36.58655405961022 40.0 35.0 41.0 28.5 41.0 50-51 36.44522377298843 40.0 35.0 41.0 28.5 41.0 52-53 36.459039532771115 39.0 35.0 41.0 29.0 41.0 54-55 36.20581133092067 39.0 35.0 41.0 29.0 41.0 56-57 35.870543692927804 39.0 35.0 41.0 29.0 41.0 58-59 35.531971159237195 38.0 35.0 41.0 28.0 41.0 60-61 35.1853949165546 37.5 35.0 40.5 27.0 41.0 62-63 34.81497803150428 37.0 34.5 40.0 26.5 41.0 64-65 34.4325047075348 36.5 34.0 39.5 26.0 41.0 66-67 34.08783868155247 36.0 34.0 39.0 25.5 41.0 68-69 33.71290828052671 35.0 34.0 39.0 25.0 41.0 70-71 33.352411066285356 35.0 34.0 37.5 25.0 40.0 72-73 33.02298780401704 35.0 34.0 37.0 25.0 39.0 74-75 32.7032427792189 35.0 34.0 36.5 25.0 39.0 76-77 32.40720459786536 35.0 34.0 36.0 25.0 38.5 78-79 32.15723013940837 35.0 33.5 36.0 24.5 37.0 80-81 31.933339756789138 35.0 33.0 35.0 24.0 37.0 82-83 31.732508473008888 35.0 33.0 35.0 24.0 36.5 84-85 31.558112132495957 35.0 33.0 35.0 24.0 36.0 86-87 31.40172486400686 35.0 33.0 35.0 23.0 36.0 88-89 31.279289989404067 35.0 33.0 35.0 23.0 36.0 90-91 31.160612767227526 35.0 33.0 35.0 21.0 35.0 92-93 31.039158577127015 35.0 33.0 35.0 20.0 35.0 94-95 30.917464476489737 35.0 33.0 35.0 19.0 35.0 96-97 30.76599945788248 35.0 33.0 35.0 17.5 35.0 98-99 30.59381830978389 35.0 33.0 35.0 6.0 35.0 100-101 29.921164331657423 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18788063893968854 1101 2 0.19445965715322444 1101 3 0.20432798385173356 1101 4 0.11017768892644852 1101 5 0.15240092386907378 1101 6 0.15778697029040245 1101 7 0.13160056464516856 1101 8 0.13650704527240265 1101 9 0.19132123845752602 1101 10-11 0.19237769554534623 1101 12-13 0.145591615278434 1101 14-15 0.20930768696944568 1101 16-17 0.23867975204256453 1101 18-19 0.23040067590023483 1101 20-21 0.23727845640290468 1101 22-23 0.2471217072226679 1101 24-25 0.23226817855196202 1101 26-27 0.2283875139604632 1101 28-29 0.25045855618766666 1101 30-31 0.2943795952466459 1101 32-33 0.30365916524221603 1101 34-35 0.32804091839614813 1101 36-37 0.3003234062070774 1101 38-39 0.2738602221012556 1101 40-41 0.11892654292682181 1101 42-43 0.11850595245348927 1101 44-45 0.16771683097938705 1101 46-47 0.17737267074188878 1101 48-49 0.25994034241669794 1101 50-51 0.2664190395106516 1101 52-53 0.24169343369555207 1101 54-55 0.1912620704239245 1101 56-57 0.20455331705326074 1101 58-59 0.24527582595067088 1101 60-61 0.20542235316948165 1101 62-63 0.15344949113149653 1101 64-65 0.10393839210856015 1101 66-67 0.14098402825010936 1101 68-69 0.16619496683340884 1101 70-71 0.17164357329917834 1101 72-73 0.1791832683247918 1101 74-75 0.14417295710831723 1101 76-77 0.08449984546209777 1101 78-79 0.06371842758005641 1101 80-81 0.0804483768296862 1101 82-83 0.054798375980414704 1101 84-85 0.09002490919847261 1101 86-87 0.11318977968136679 1101 88-89 0.05631776028809554 1101 90-91 0.02362524221261353 1101 92-93 0.015136867673327004 1101 94-95 0.044012691709458096 1101 96-97 0.08639332468499816 1101 98-99 0.1048589030089353 1101 100-101 0.23068079919985252 1102 1 0.1412555543459746 1102 2 0.15918624240008228 1102 3 0.11823782662546023 1102 4 0.06635794829470854 1102 5 0.09401874914173902 1102 6 0.12640677466252015 1102 7 0.0864220325625098 1102 8 0.07610502840087463 1102 9 0.11379853586541344 1102 10-11 0.13901449538481359 1102 12-13 0.16904757343012022 1102 14-15 0.25451520715389364 1102 16-17 0.21437284014593416 1102 18-19 0.21646292703732684 1102 20-21 0.19843564922935997 1102 22-23 0.19458415154820585 1102 24-25 0.2216455589797306 1102 26-27 0.2356931451476143 1102 28-29 0.222077866768565 1102 30-31 0.2510248938034394 1102 32-33 0.29838042895426753 1102 34-35 0.2588411355855911 1102 36-37 0.2874510266449164 1102 38-39 0.23311766634809317 1102 40-41 0.09012895404440968 1102 42-43 0.12443496849088831 1102 44-45 0.20619408510677317 1102 46-47 0.17992814847997352 1102 48-49 0.19287385569199955 1102 50-51 0.24150118394163655 1102 52-53 0.19573681649981722 1102 54-55 0.2127234858538145 1102 56-57 0.23453299488891588 1102 58-59 0.2663234600808906 1102 60-61 0.20744096575387516 1102 62-63 0.1928727095485243 1102 64-65 0.21227403045665483 1102 66-67 0.14781040653745947 1102 68-69 0.1278506428748969 1102 70-71 0.06055421282765394 1102 72-73 0.1248374531561538 1102 74-75 0.11682030755450512 1102 76-77 0.10694001016408805 1102 78-79 0.112767171265034 1102 80-81 0.06698049686619356 1102 82-83 0.041690294723235155 1102 84-85 0.05544073648565018 1102 86-87 0.07927953361202356 1102 88-89 0.0842668643816431 1102 90-91 0.11998837155050168 1102 92-93 0.008630253625266704 1102 94-95 0.07295402105049575 1102 96-97 0.09140569897480333 1102 98-99 0.06192881282809992 1102 100-101 0.18355975936604807 1103 1 0.1734524772983974 1103 2 0.1735198353571974 1103 3 0.18053890254687133 1103 4 0.08921799084603066 1103 5 0.11655625129122171 1103 6 0.10604188523865332 1103 7 0.09545275920564222 1103 8 0.09886890900090606 1103 9 0.155665332006798 1103 10-11 0.1458102995947641 1103 12-13 0.14336123941906465 1103 14-15 0.23873935351426923 1103 16-17 0.22946584952273952 1103 18-19 0.22149406503243085 1103 20-21 0.2097192022313834 1103 22-23 0.21474285922868575 1103 24-25 0.23930695562080473 1103 26-27 0.2646104802612186 1103 28-29 0.23427371076501657 1103 30-31 0.24877446137811177 1103 32-33 0.2612241522964638 1103 34-35 0.2656940907448515 1103 36-37 0.29372933659524847 1103 38-39 0.202770748284685 1103 40-41 0.022685573945075532 1103 42-43 0.029075119814464756 1103 44-45 0.0804028226271285 1103 46-47 0.14259856451865716 1103 48-49 0.1400886319940895 1103 50-51 0.1725113227560655 1103 52-53 0.22561951375487865 1103 54-55 0.16536328651670118 1103 56-57 0.22597377200695945 1103 58-59 0.21770297272996686 1103 60-61 0.224065688007002 1103 62-63 0.19825643012590888 1103 64-65 0.23146555525747203 1103 66-67 0.16169903130691665 1103 68-69 0.129683755359423 1103 70-71 0.1152914027542522 1103 72-73 0.12252922915140374 1103 74-75 0.14220399441450127 1103 76-77 0.13843264460368943 1103 78-79 0.1373632876978732 1103 80-81 0.09257060149121088 1103 82-83 0.09868978559088859 1103 84-85 0.15302707825170003 1103 86-87 0.09812329741507497 1103 88-89 0.0656282033660105 1103 90-91 0.05690420961269638 1103 92-93 0.11142107440730342 1103 94-95 0.14632505040821187 1103 96-97 0.17631059912080005 1103 98-99 0.15971061421253552 1103 100-101 0.22844587893460044 1104 1 0.17760644116936675 1104 2 0.17016434509071132 1104 3 0.15200655207949865 1104 4 0.07325402351387567 1104 5 0.10374370094326224 1104 6 0.09384240598872395 1104 7 0.12125401214522213 1104 8 0.09144946048795788 1104 9 0.13815739366394553 1104 10-11 0.14202084147800065 1104 12-13 0.13830297723472285 1104 14-15 0.19173791699953568 1104 16-17 0.16512754777677685 1104 18-19 0.19801346467788505 1104 20-21 0.1884267004660316 1104 22-23 0.2056983154678207 1104 24-25 0.19157574147160972 1104 26-27 0.1857452366708756 1104 28-29 0.20235634580283346 1104 30-31 0.20712638745591505 1104 32-33 0.27784908779291584 1104 34-35 0.2943594633962334 1104 36-37 0.3084178742881747 1104 38-39 0.3111693995396223 1104 40-41 0.13378611062488943 1104 42-43 0.1551731699895882 1104 44-45 0.11024390084229907 1104 46-47 0.15987893504672712 1104 48-49 0.3490209127496655 1104 50-51 0.305924770175217 1104 52-53 0.29694675791829184 1104 54-55 0.2297545644609329 1104 56-57 0.16376883339948023 1104 58-59 0.18708302806242472 1104 60-61 0.21093702553428528 1104 62-63 0.15299103526254498 1104 64-65 0.13008837840249043 1104 66-67 0.1448046322502421 1104 68-69 0.17507269514368318 1104 70-71 0.13901192825316855 1104 72-73 0.10460951223895876 1104 74-75 0.1509104070201488 1104 76-77 0.12932952057429503 1104 78-79 0.12217686737876221 1104 80-81 0.13904030395792688 1104 82-83 0.18444386446021355 1104 84-85 0.19105743398218777 1104 86-87 0.17324436945129307 1104 88-89 0.16025545494665394 1104 90-91 0.18081565088819573 1104 92-93 0.18700563852215168 1104 94-95 0.18974005221201296 1104 96-97 0.20507800841331658 1104 98-99 0.16044471904048407 1104 100-101 0.2147854540388181 1105 1 0.12366520355418942 1105 2 0.13703089007255898 1105 3 0.16536389652417682 1105 4 0.0802763093253489 1105 5 0.10025073483895142 1105 6 0.11069814741130557 1105 7 0.0929240816975394 1105 8 0.08051389774776396 1105 9 0.14617178370562556 1105 10-11 0.134740949381694 1105 12-13 0.14481679750572596 1105 14-15 0.21725587409995484 1105 16-17 0.18067214885390115 1105 18-19 0.20139507016493496 1105 20-21 0.14930646929257563 1105 22-23 0.19760947258249217 1105 24-25 0.20132895315447996 1105 26-27 0.2176278174153552 1105 28-29 0.211056064987865 1105 30-31 0.22345133025579145 1105 32-33 0.21884677473951086 1105 34-35 0.2489034116047577 1105 36-37 0.257824160657357 1105 38-39 0.24038296360085099 1105 40-41 0.03310026333185334 1105 42-43 0.0650191166099745 1105 44-45 0.08664992769331548 1105 46-47 0.15319943156822546 1105 48-49 0.16315112038249424 1105 50-51 0.22887172954823143 1105 52-53 0.20636729534314213 1105 54-55 0.13247825286201476 1105 56-57 0.09475444163985713 1105 58-59 0.08421811201014862 1105 60-61 0.10412308853828733 1105 62-63 0.025781521746353064 1105 64-65 -0.012608795143677298 1105 66-67 0.017146753014138483 1105 68-69 0.008236147425890294 1105 70-71 -0.022706256622520016 1105 72-73 0.038273109187450416 1105 74-75 0.02937090564417133 1105 76-77 0.01367331781562342 1105 78-79 0.021894497972837712 1105 80-81 -0.01207271479371741 1105 82-83 0.006001262781378358 1105 84-85 0.021735652006160677 1105 86-87 -0.01942263226701968 1105 88-89 0.015962615924788537 1105 90-91 0.04028157212867356 1105 92-93 -0.015972804955111997 1105 94-95 -0.024465240019136303 1105 96-97 -0.01878285061012619 1105 98-99 -0.08007247690637342 1105 100-101 -0.045147378246550574 1106 1 0.11586644765707632 1106 2 0.05349374703285292 1106 3 0.08199796518972136 1106 4 0.06869947939251375 1106 5 0.08894932733090144 1106 6 0.07190325002732578 1106 7 0.05246997132604747 1106 8 0.06531852724151577 1106 9 0.09186403727620274 1106 10-11 0.11167277804472064 1106 12-13 0.0983530759583573 1106 14-15 0.17807159666100603 1106 16-17 0.1469970870287085 1106 18-19 0.18824070274575178 1106 20-21 0.1614443467439557 1106 22-23 0.16863054450079318 1106 24-25 0.11624015609111638 1106 26-27 0.17763476750413787 1106 28-29 0.1235067641211387 1106 30-31 0.17037956429427936 1106 32-33 0.22684099680698466 1106 34-35 0.24954722663944295 1106 36-37 0.25911412366783537 1106 38-39 0.24892805308019206 1106 40-41 0.18478110002110526 1106 42-43 0.18203700598145645 1106 44-45 0.23854879284606767 1106 46-47 0.17776350099005356 1106 48-49 0.2820026608546442 1106 50-51 0.2565399688969876 1106 52-53 0.2506259092205312 1106 54-55 0.2691265420896798 1106 56-57 0.26785471911996694 1106 58-59 0.23944483417645301 1106 60-61 0.22882171947639307 1106 62-63 0.2638790231619126 1106 64-65 0.23717993154338046 1106 66-67 0.20092060609606222 1106 68-69 0.21595097159251964 1106 70-71 0.23895294824255586 1106 72-73 0.22275600940597684 1106 74-75 0.21240012634076066 1106 76-77 0.1800617101836437 1106 78-79 0.20662918435787248 1106 80-81 0.17792162568067482 1106 82-83 0.20720539431629703 1106 84-85 0.20793708148085344 1106 86-87 0.19443742050708224 1106 88-89 0.1721942671398935 1106 90-91 0.1936484980090114 1106 92-93 0.16494447229232634 1106 94-95 0.21152922102814742 1106 96-97 0.2724764989785271 1106 98-99 0.23649025881318408 1106 100-101 0.3292495542201763 1107 1 0.05695973170416835 1107 2 0.09770182344760059 1107 3 0.07943948604246742 1107 4 0.02195802330694363 1107 5 0.04312514302000636 1107 6 0.03351711125706203 1107 7 0.0488085193827672 1107 8 0.019054838812024855 1107 9 0.044823230442119666 1107 10-11 0.05861551262390208 1107 12-13 0.06964945513948351 1107 14-15 0.0941333308417498 1107 16-17 0.0226164561015878 1107 18-19 0.017041298814433503 1107 20-21 0.04441000785187299 1107 22-23 0.03941361306097946 1107 24-25 0.05761708177384861 1107 26-27 0.014138928225037262 1107 28-29 -0.023914878999413247 1107 30-31 0.03519580277792045 1107 32-33 0.0795036914376297 1107 34-35 0.05308778862198693 1107 36-37 0.11319248142488902 1107 38-39 0.07700892605051735 1107 40-41 -0.059922822693572186 1107 42-43 0.01629787905709179 1107 44-45 0.034698963189022436 1107 46-47 0.031025248159345153 1107 48-49 0.050314703513215875 1107 50-51 0.03000789159059991 1107 52-53 -0.06979826315495075 1107 54-55 -0.024886048018323947 1107 56-57 -0.06182269117573469 1107 58-59 -0.029423346181715715 1107 60-61 -0.07913907092893879 1107 62-63 -0.07593642547806212 1107 64-65 -0.12931700649601652 1107 66-67 -0.12081979686462319 1107 68-69 -0.12395175477227838 1107 70-71 -0.10084494831600921 1107 72-73 -0.16029964999939494 1107 74-75 -0.20379026459674776 1107 76-77 -0.08697091738049068 1107 78-79 -0.14634896505598505 1107 80-81 -0.1633126454603726 1107 82-83 -0.10938658824395375 1107 84-85 -0.08604400264100676 1107 86-87 -0.05253695169884409 1107 88-89 -0.09366956732291598 1107 90-91 -0.09471850899251422 1107 92-93 -0.050444534488040205 1107 94-95 -0.08860632492057263 1107 96-97 -0.06698172092448118 1107 98-99 -0.056639505479914476 1107 100-101 -0.04772939907900309 1108 1 0.033160933830693295 1108 2 0.049412676275430556 1108 3 0.0714111522572125 1108 4 0.022292346771827454 1108 5 0.046070168122511745 1108 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-0.04739909871854309 2214 1 -0.1295145641757145 2214 2 -0.1339278637997694 2214 3 -0.10446686019660945 2214 4 -0.06818668991450494 2214 5 -0.10262389345074752 2214 6 -0.1136132367828111 2214 7 -0.1473388028278393 2214 8 -0.0829730532205275 2214 9 -0.1548910445424596 2214 10-11 -0.15075563148822368 2214 12-13 -0.15751209642876063 2214 14-15 -0.188364066802869 2214 16-17 -0.2182368241254835 2214 18-19 -0.22510876281231162 2214 20-21 -0.17728254969284052 2214 22-23 -0.2290262082720531 2214 24-25 -0.17812852086959197 2214 26-27 -0.1578766946773129 2214 28-29 -0.21047860730384826 2214 30-31 -0.20673710918634924 2214 32-33 -0.19380852172142227 2214 34-35 -0.24335269069263177 2214 36-37 -0.20979533961941144 2214 38-39 -0.22044717459233 2214 40-41 -0.05051608101340577 2214 42-43 -0.08350763858844346 2214 44-45 -0.1175577944459647 2214 46-47 -0.11226100103757375 2214 48-49 -0.06258640158550577 2214 50-51 -0.06410374670033292 2214 52-53 -0.14990745301680874 2214 54-55 -0.1123723897284421 2214 56-57 -0.12891169386590207 2214 58-59 -0.16665218457437447 2214 60-61 -0.11786179285886078 2214 62-63 -0.06859728859429026 2214 64-65 -0.1041245536301858 2214 66-67 -0.039024905868963344 2214 68-69 -0.051266153257707 2214 70-71 -0.010611274905585333 2214 72-73 0.03065347432247023 2214 74-75 0.005663644423243852 2214 76-77 -0.01454558513464832 2214 78-79 0.05328976347894354 2214 80-81 0.0367191629792849 2214 82-83 0.01714937485690271 2214 84-85 0.003978334102093584 2214 86-87 -0.03177600252331203 2214 88-89 -0.03943901111709991 2214 90-91 0.0016958624405596368 2214 92-93 -0.09411127064080205 2214 94-95 -0.1219139101708997 2214 96-97 -0.06138043347535671 2214 98-99 -0.04145995364048005 2214 100-101 -0.1211086022286807 2215 1 -0.16514653635918108 2215 2 -0.1897703322971438 2215 3 -0.15985633219720796 2215 4 -0.11749690496277054 2215 5 -0.11266842370376651 2215 6 -0.13000811313167304 2215 7 -0.1615216448275021 2215 8 -0.16381903706471945 2215 9 -0.18180982695518821 2215 10-11 -0.17520179854736284 2215 12-13 -0.21455920131798933 2215 14-15 -0.23893823379832924 2215 16-17 -0.3011660252302306 2215 18-19 -0.2619626070098633 2215 20-21 -0.2557192418026588 2215 22-23 -0.2990331461074618 2215 24-25 -0.23152572928626114 2215 26-27 -0.1820721824501632 2215 28-29 -0.2186307913101686 2215 30-31 -0.26744847514589054 2215 32-33 -0.30806556501723037 2215 34-35 -0.30282075144540954 2215 36-37 -0.307635893342443 2215 38-39 -0.22036063641869674 2215 40-41 -0.01414392460681313 2215 42-43 -0.03439610222459066 2215 44-45 0.017447621651712097 2215 46-47 -0.07439495489120418 2215 48-49 -0.21025063839897484 2215 50-51 -0.20915933087137972 2215 52-53 -0.19063524730061232 2215 54-55 -0.08752625010986037 2215 56-57 -0.07584290032914254 2215 58-59 -0.08606281157201323 2215 60-61 -0.05755830188259381 2215 62-63 -0.11719223953292612 2215 64-65 -0.07134014501764341 2215 66-67 -0.03689633968701145 2215 68-69 0.05404289215601921 2215 70-71 0.025121154708827476 2215 72-73 0.006187854414278604 2215 74-75 -0.050467480476363846 2215 76-77 -0.08594029850786455 2215 78-79 0.010646863209807123 2215 80-81 0.050104798229934744 2215 82-83 0.08379075187226093 2215 84-85 0.08187135082301467 2215 86-87 0.04611529916318702 2215 88-89 0.02418846293850052 2215 90-91 0.03440178690854978 2215 92-93 0.027230475647051833 2215 94-95 0.03337189226244064 2215 96-97 -1.2586589333096754E-4 2215 98-99 0.04273495234721025 2215 100-101 -0.0035254723306437086 2216 1 -0.301650771096881 2216 2 -0.25749144967211635 2216 3 -0.2607149097089234 2216 4 -0.17274477637201358 2216 5 -0.1982386901525217 2216 6 -0.2517305526507272 2216 7 -0.2547555521101046 2216 8 -0.2487899519353718 2216 9 -0.31140636897713136 2216 10-11 -0.2975305778288799 2216 12-13 -0.3053963143919134 2216 14-15 -0.42347608527339986 2216 16-17 -0.45951843579677387 2216 18-19 -0.4564509698976025 2216 20-21 -0.47642569113168776 2216 22-23 -0.5105987952536779 2216 24-25 -0.4329883511714172 2216 26-27 -0.4589468521691842 2216 28-29 -0.4167051639548447 2216 30-31 -0.42416335518375803 2216 32-33 -0.5474563279637294 2216 34-35 -0.5321890297812217 2216 36-37 -0.5473451032593388 2216 38-39 -0.45447496311478375 2216 40-41 -0.22235004237965228 2216 42-43 -0.16268784310884143 2216 44-45 -0.1971292718401898 2216 46-47 -0.3239957612358637 2216 48-49 -0.5385612524818555 2216 50-51 -0.6240836442440099 2216 52-53 -0.5948069893274521 2216 54-55 -0.4352995697320665 2216 56-57 -0.38374852617460675 2216 58-59 -0.40414660041254535 2216 60-61 -0.27054959164638603 2216 62-63 -0.19512268618503015 2216 64-65 -0.13488406244076856 2216 66-67 -0.06297037771497571 2216 68-69 -0.09427994359818115 2216 70-71 -0.1832356936098236 2216 72-73 -0.19631483374131165 2216 74-75 -0.11431642959625776 2216 76-77 -0.12239296350552564 2216 78-79 -0.035972385820521424 2216 80-81 -0.02001418453526327 2216 82-83 -0.09422844982204381 2216 84-85 -0.04778303500229342 2216 86-87 -0.005598234246459555 2216 88-89 -0.011836086502135856 2216 90-91 0.002411256153397545 2216 92-93 0.030272682240223503 2216 94-95 -0.08930619421965602 2216 96-97 -0.1599274830714137 2216 98-99 -0.0854953741285236 2216 100-101 -0.2223299623156052 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 17.0 13 126.0 14 540.0 15 1488.0 16 3151.0 17 7360.0 18 20567.0 19 82466.0 20 96827.0 21 73027.0 22 60715.0 23 51629.0 24 49599.0 25 53213.0 26 64398.0 27 83303.0 28 104675.0 29 120682.0 30 133965.0 31 154562.0 32 185463.0 33 239077.0 34 333686.0 35 486595.0 36 655603.0 37 1649314.0 38 2145688.0 39 365598.0 40 192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.51823411346394 28.417927053013763 21.3567008040502 21.70713802947209 2 20.56433381703063 22.62467110937235 38.721532946652374 18.089462126944653 3 19.845598949875725 27.490328130611008 20.55298202013809 32.111090899375185 4 31.94761118046782 19.93102814331948 26.882384032396367 21.23897664381633 5 21.192932648127798 31.980296049325496 20.60313758128648 26.223633721260224 6 21.57230416281467 20.26131005827348 25.836800476664724 32.32958530224713 7 33.252347953063364 20.56069293583217 19.798419774497937 26.388539336606527 8 39.30012849680336 20.48520348649676 19.966800147185737 20.247867869514142 9 20.87378103159039 26.49426332790939 20.25310077100851 32.37885486949171 10-11 33.20639394113069 20.237588955864492 19.896079836910673 26.659937266094147 12-13 29.837132170632458 20.328846327956736 19.993400729782103 29.8406207716287 14-15 29.57758440960827 20.563600103329037 20.181037626222984 29.677777860839704 16-17 29.467257403102032 26.330562110526078 20.410330079797596 23.791850406574298 18-19 23.330635205023142 20.738500837402675 26.46708131181365 29.463782645760535 20-21 23.035959170078435 29.53959049915512 21.158503478772 26.265946851994443 22-23 19.631195346981517 26.158347045473363 30.753782847877893 23.45667475966723 24-25 19.655843974258556 20.34791596237073 36.78798968813845 23.20825037523226 26-27 19.676387957903106 20.31966798485947 40.31620430244177 19.68773975479565 28-29 19.696218993328188 20.25467202582229 40.38783137210277 19.66127760874675 30-31 19.676775580236022 20.204080389549368 40.38133177619905 19.73781225401556 32-33 19.694862315162982 20.25265085222923 40.379774365040014 19.672712467567777 34-35 19.703016227808966 20.301069034706874 40.33298973382251 19.66292500366165 36-37 19.74624996158386 20.292299079424648 40.19674048380251 19.764710475188988 38-39 20.141368633545444 20.520933959398775 39.00821427098069 20.329483136075098 40-41 22.16793433013185 22.182982382841843 32.91947035284431 22.729612934182004 42-43 22.03653728110067 22.580918238544445 33.75271993206642 21.629824548288468 44-45 22.47554726043763 23.438456510020174 32.45799212185296 21.628004107689236 46-47 22.14602674649472 24.354331942599778 31.62447259136328 21.87516871954223 48-49 22.444025258578705 25.097320892871434 29.753869509156612 22.704784339393257 50-51 22.604237874965772 25.50880968657135 29.053830497737533 22.833121940725345 52-53 22.713055923104587 25.276443941642906 29.12430162222715 22.886198513025356 54-55 22.82192242403502 24.82116074615084 29.26336805598817 23.093548773825965 56-57 22.82338292961083 24.70107396304796 29.39518041466176 23.080362692679447 58-59 22.870735150672957 24.721825601513718 29.36664172040081 23.04079752741251 60-61 22.936977038637362 24.802534108688747 28.99861508077911 23.261873771894777 62-63 23.243323274533793 24.921575695858227 28.41461358344941 23.420487446158567 64-65 23.442014329290156 25.11596137398827 27.77635880316621 23.665665493555363 66-67 23.609093398431735 25.290086863396077 27.249517756286888 23.8513019818853 68-69 23.724438729783763 25.55350392592205 26.81632211194367 23.905735232350516 70-71 23.895788566414794 25.704448215456 26.512869199889362 23.886894018239847 72-73 24.005575670385902 25.738808166538057 26.343145992691102 23.912470170384932 74-75 23.981882255286408 25.783474718579264 26.290076342218466 23.94456668391586 76-77 23.967097232016606 25.823330600595884 26.192457810769977 24.01711435661753 78-79 24.064701919810354 25.713287389011956 26.119204111676208 24.10280657950148 80-81 24.075936045637548 25.73288308230634 26.099054672191947 24.09212619986417 82-83 24.135339168156936 25.72805856862701 26.05020733641715 24.086394926798906 84-85 24.13734649809525 25.642193300058725 26.05211776077223 24.168342441073793 86-87 24.21653912507548 25.632696552902278 25.980982140854756 24.16978218116748 88-89 24.252359581733355 25.658542656314935 25.91090556052543 24.178192201426285 90-91 24.325855544785192 25.624210392542366 25.79877887890208 24.251155183770365 92-93 24.289889729752478 25.661498276603425 25.709376556545934 24.339235437098168 94-95 24.273401936948797 25.70393600023036 25.612394833215802 24.410267229605044 96-97 24.241921466054112 25.647440045207837 25.572469732925445 24.538168755812602 98-99 24.260340448695 25.68217377496807 25.579516153191665 24.477969623145263 100-101 24.311783469734863 25.57084310349267 25.468157794406775 24.64921563236569 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2782.0 1 1633.0 2 379.0 3 231.0 4 168.5 5 141.0 6 127.5 7 141.0 8 170.0 9 164.5 10 165.5 11 175.0 12 181.5 13 243.5 14 332.5 15 634.5 16 6874.5 17 11241.0 18 10627.5 19 10684.5 20 9211.0 21 8808.0 22 9372.5 23 10601.0 24 12269.0 25 14588.0 26 18069.5 27 23156.5 28 30178.0 29 39295.0 30 50956.0 31 64782.0 32 79254.5 33 94571.5 34 110562.0 35 126709.0 36 143317.0 37 160894.5 38 179027.0 39 195033.0 40 206160.5 41 212405.0 42 218919.0 43 227967.0 44 240216.0 45 257104.5 46 273419.5 47 286159.5 48 295230.0 49 297666.0 50 292507.5 51 286403.0 52 286550.0 53 286953.5 54 277216.5 55 260940.0 56 243292.0 57 222870.5 58 202805.5 59 182441.0 60 157450.5 61 133519.5 62 111185.5 63 85765.0 64 63335.5 65 46520.0 66 33301.0 67 24421.0 68 18689.0 69 14760.5 70 11903.5 71 9546.0 72 7914.5 73 6184.5 74 4631.5 75 3545.0 76 2821.0 77 2099.0 78 1231.5 79 604.5 80 273.5 81 130.0 82 67.0 83 28.5 84 7.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10636079942122449 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7223526.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.501809218805676 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9103426568841 43.0075644087608 2 11.209165559896121 12.21839605694931 3 3.737034872907434 6.110254850616741 4 1.7191721278669037 3.7479196530916066 5 1.0222889752160653 2.7858299346857183 6 0.6263739057092139 2.0483106665161053 7 0.4283915202109614 1.6343679033844367 8 0.3428811106183533 1.495011270052296 9 0.25595608994862123 1.2555062984494096 >10 1.6839192157384841 16.574478404274124 >50 0.050865399400295275 1.7524297400638833 >100 0.010674286358148396 1.1221254845228001 >500 0.001264833488790624 0.4863329080355844 >1k 0.0012647316373988122 1.4773663759863296 >5k 2.7824095687142267E-4 1.186067504326694 >10k+ 1.2647316221428302E-4 3.0980385402841626 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 161641 2.237702196960321 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 19350 0.2678747193545091 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 18923 0.26196347877753884 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 14221 0.19687061415713047 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT 10588 0.14657661646126835 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 9902 0.13707986930482427 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 9799 0.13565397286588296 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCG 9170 0.12694631403001802 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCA 8926 0.12356846227174927 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8844 0.12243328258249504 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 8551 0.11837709174162313 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG 8170 0.11310265928301498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3843654747003056E-5 2 2.768730949400611E-5 0.0 0.0 0.0 2.768730949400611E-5 3 2.768730949400611E-5 0.0 0.0 0.0 2.768730949400611E-5 4 2.768730949400611E-5 0.0 0.0 0.0 2.768730949400611E-5 5 2.768730949400611E-5 0.0 0.0 0.0 4.153096424100917E-5 6 4.153096424100917E-5 0.0 0.0 0.0 5.537461898801222E-5 7 4.153096424100917E-5 0.0 0.0 0.0 6.921827373501529E-5 8 4.153096424100917E-5 0.0 0.0 0.0 8.306192848201834E-5 9 1.1074923797602445E-4 0.0 0.0 0.0 8.306192848201834E-5 10-11 3.8070050554258407E-4 0.0 0.0 0.0 8.306192848201834E-5 12-13 7.198700468441589E-4 0.0 0.0 0.0 8.306192848201834E-5 14-15 7.821664932056726E-4 0.0 0.0 0.0 9.69055832290214E-5 16-17 8.098538026996788E-4 0.0 0.0 0.0 1.3843654747003057E-4 18-19 0.001072883242892737 6.921827373501528E-6 0.0 0.0 1.5228020221703362E-4 20-21 0.0015228020221703363 1.3843654747003056E-5 0.0 0.0 1.5228020221703362E-4 22-23 0.0019035025277129203 1.3843654747003056E-5 0.0 0.0 1.661238569640367E-4 24-25 0.0020765482120504585 1.3843654747003056E-5 0.0 0.0 1.661238569640367E-4 26-27 0.0022149847595204887 1.3843654747003056E-5 0.0 0.0 1.7304568433753822E-4 28-29 0.002443405062846039 1.3843654747003056E-5 0.0 0.0 1.938111664580428E-4 30-31 0.0025956852650630734 1.3843654747003056E-5 0.0 0.0 2.214984759520489E-4 32-33 0.002609528919810076 1.3843654747003056E-5 0.0 0.0 2.3534213069905196E-4 34-35 0.002644138056677584 1.3843654747003056E-5 0.0 0.0 2.49185785446055E-4 36-37 0.0027341218125331032 2.0765482120504583E-5 0.0 1.3843654747003056E-5 2.5610761281955654E-4 38-39 0.002782574604147614 2.768730949400611E-5 0.0 1.3843654747003056E-5 3.0456040443406723E-4 40-41 0.0028794801873766357 2.768730949400611E-5 0.0 1.3843654747003056E-5 3.2532588655457184E-4 42-43 0.002921011151617645 2.768730949400611E-5 0.0 1.3843654747003056E-5 3.322477139280734E-4 44-45 0.002921011151617645 2.768730949400611E-5 0.0 1.3843654747003056E-5 3.322477139280734E-4 46-47 0.0029417766337381497 2.768730949400611E-5 0.0 1.3843654747003056E-5 3.5301319604857793E-4 48-49 0.0032048060739312077 2.768730949400611E-5 0.0 1.3843654747003056E-5 3.8762233291608555E-4 50-51 0.004540718757017002 2.768730949400611E-5 0.0 1.3843654747003056E-5 4.153096424100917E-4 52-53 0.00475529540559555 2.768730949400611E-5 0.0 1.3843654747003056E-5 4.2915329715709476E-4 54-55 0.005039090327909113 2.768730949400611E-5 0.0 1.3843654747003056E-5 4.8452791614510694E-4 56-57 0.005378259869210688 2.768730949400611E-5 0.0 1.3843654747003056E-5 5.329807077596177E-4 58-59 0.00566205479152425 2.768730949400611E-5 0.0 1.3843654747003056E-5 5.537461898801223E-4 60-61 0.0060981299160548464 2.768730949400611E-5 0.0 1.3843654747003056E-5 5.952771541211314E-4 62-63 0.006617266969067461 2.768730949400611E-5 0.0 1.3843654747003056E-5 6.229644636151375E-4 64-65 0.007517104527622659 2.768730949400611E-5 0.0 1.3843654747003056E-5 6.368081183621406E-4 66-67 0.008527691324153883 2.768730949400611E-5 0.0 1.3843654747003056E-5 6.437299457356421E-4 68-69 0.009939744108348195 2.768730949400611E-5 0.0 1.3843654747003056E-5 6.783390826031498E-4 70-71 0.01154560805900055 2.768730949400611E-5 0.0 1.3843654747003056E-5 6.921827373501528E-4 72-73 0.013892107538617568 2.768730949400611E-5 0.0 2.768730949400611E-5 6.921827373501528E-4 74-75 0.016785431380741206 2.768730949400611E-5 0.0 2.768730949400611E-5 6.921827373501528E-4 76-77 0.020717029328890073 2.768730949400611E-5 0.0 2.768730949400611E-5 7.198700468441589E-4 78-79 0.025839181585281205 2.768730949400611E-5 0.0 2.768730949400611E-5 7.33713701591162E-4 80-81 0.03301711657160229 2.768730949400611E-5 0.0 2.768730949400611E-5 7.614010110851681E-4 82-83 0.041586338859997185 2.768730949400611E-5 0.0 2.768730949400611E-5 7.614010110851681E-4 84-85 0.05225287484256304 2.768730949400611E-5 0.0 3.460913686750764E-5 7.614010110851681E-4 86-87 0.06602038948845758 2.768730949400611E-5 0.0 4.8452791614510694E-5 7.614010110851681E-4 88-89 0.08337341071382591 2.768730949400611E-5 0.0 6.229644636151375E-5 7.683228384586697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 84630 0.0 93.60673 1 TCGACGG 86795 0.0 91.39806 2 ATATCTC 49055 0.0 90.54425 1 TATCTCG 49930 0.0 88.69178 2 CGACGGC 89405 0.0 88.6555 3 ACGGCGC 90450 0.0 87.63648 5 GACGGCG 90720 0.0 87.41755 4 CGGCGCG 91805 0.0 86.30163 6 ATCTCGA 53565 0.0 82.69962 3 TCTCGAG 54020 0.0 80.49068 4 CTCGAGG 56180 0.0 77.35371 5 TCGAGGG 56250 0.0 77.29122 6 CGAGGGC 57250 0.0 76.04072 7 AGGGCGC 58025 0.0 75.819145 9 GAGGGCG 58715 0.0 74.782524 8 CGCGCCG 148320 0.0 54.5098 9 GGCGCGC 149570 0.0 54.20668 7 GCGCGCC 149340 0.0 54.188385 8 CTCGACG 1865 0.0 42.787773 5 TGTCGAC 1750 0.0 42.654602 1 >>END_MODULE