FastQCFastQC Report
Tue 31 May 2016
SRR1567933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16809518
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT12004107.141251759866048No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT2283471.3584387131147961No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070800.6370200501882327No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT704180.4189174252349175No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445410.264974879113131No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA265900.15818419064722736No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256850.1528003360953003No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT242200.14408503563278852No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172630.10269776920432817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1447700.094.390891
TCGACGG1476650.092.537112
ATATCTC1433000.091.963121
TATCTCG1432500.091.56892
CGACGGC1521850.089.597533
ACGGCGC1565850.087.35615
GACGGCG1568700.087.197224
CGGCGCG1575700.086.818766
ATCTCGA1565350.083.669463
TCTCGAG1600000.080.8840564
CTCGAGG1680050.076.9196855
TCGAGGG1692600.076.358136
CGAGGGC1710000.075.461627
AGGGCGC1765350.073.880659
GAGGGCG1776150.073.302258
CGCGCCG3336150.043.080149
GGCGCGC3356550.043.0410167
GCGCGCC3361000.042.9408348
TCCATAT1647600.041.1189218-19
CCATATT1652600.041.09075518-19
Produced by FastQC (version 0.11.5)