FastQCFastQC Report
Tue 31 May 2016
SRR1567933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16809518
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1366080.8126824338449203No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1061720.6316183486046417No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT783570.4661466200280103No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509830.3032984051059644No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298490.17757201604471942No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT189910.11297765944270383No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT175380.10433374710684745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG62850.084.280111
TCGACGG79100.067.6797942
ATCTCGA232450.063.3968621
TCTCGAG286750.051.1381262
CGACGGC126700.042.215643
CTCGAGG378600.038.6188773
TCGAGGG387250.037.793044
CGAGGGC409400.035.827935
TATCTCG65100.033.057711
GGGCGCG445400.032.9412848
AGGGCGC448100.032.7972647
ACGGCGC167250.032.1777765
GACGGCG171900.031.1429444
GAGGGCG480300.030.6578276
CGGCGCG177650.030.3742356
ATATCTC70700.030.1033021
GGCGCGC633200.023.4188129
CGCGCCG616750.022.5955969
GCGCGCC638200.021.9180228
GGATCCT530400.019.11489716-17
Produced by FastQC (version 0.11.5)