##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567933_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16809518 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67397024709453 34.0 31.0 34.0 31.0 34.0 2 32.96279221093668 34.0 31.0 34.0 31.0 34.0 3 33.07494950182391 34.0 33.0 34.0 31.0 34.0 4 36.46399379208851 37.0 37.0 37.0 35.0 37.0 5 36.40228054129809 37.0 37.0 37.0 35.0 37.0 6 36.4233547327175 37.0 37.0 37.0 35.0 37.0 7 36.41250296409451 37.0 37.0 37.0 35.0 37.0 8 36.39654105489521 37.0 37.0 37.0 35.0 37.0 9 38.234688882810325 39.0 39.0 39.0 37.0 39.0 10-11 38.23711102245764 39.0 39.0 39.0 37.0 39.0 12-13 38.19263883711598 39.0 39.0 39.0 37.0 39.0 14-15 39.73581714240706 41.0 40.0 41.0 37.0 41.0 16-17 39.70709820471949 41.0 40.0 41.0 37.0 41.0 18-19 39.6761001713434 41.0 40.0 41.0 37.0 41.0 20-21 39.66191796814162 41.0 40.0 41.0 37.0 41.0 22-23 39.62179828713708 41.0 40.0 41.0 37.0 41.0 24-25 39.58714101736885 41.0 40.0 41.0 37.0 41.0 26-27 39.46944962966815 41.0 39.0 41.0 36.0 41.0 28-29 39.29710664517567 41.0 39.0 41.0 35.5 41.0 30-31 39.147991364178324 41.0 39.0 41.0 35.0 41.0 32-33 39.06423149670324 40.0 38.5 41.0 35.0 41.0 34-35 38.98673079144804 40.0 38.0 41.0 35.0 41.0 36-37 38.88737609846993 40.0 38.0 41.0 35.0 41.0 38-39 38.76786565206688 40.0 38.0 41.0 35.0 41.0 40-41 38.65098368674224 40.0 38.0 41.0 35.0 41.0 42-43 38.56910519385505 40.0 38.0 41.0 35.0 41.0 44-45 38.466322562015165 40.0 38.0 41.0 34.5 41.0 46-47 38.31544179315552 40.0 38.0 41.0 34.0 41.0 48-49 38.39429512494053 40.0 38.0 41.0 34.0 41.0 50-51 38.42712256829732 40.0 38.0 41.0 34.5 41.0 52-53 38.45062172514405 40.0 37.0 41.0 35.0 41.0 54-55 38.3215041323612 40.0 37.0 41.0 34.5 41.0 56-57 38.09459256951924 40.0 37.0 41.0 34.0 41.0 58-59 37.82987974432105 40.0 36.0 41.0 34.0 41.0 60-61 37.520854018538785 39.0 35.5 41.0 34.0 41.0 62-63 37.19803072283215 39.0 35.0 41.0 33.0 41.0 64-65 36.85747434875884 38.5 35.0 41.0 33.0 41.0 66-67 36.49728350331044 37.5 35.0 40.0 33.0 41.0 68-69 36.11962291244758 37.0 35.0 40.0 33.0 41.0 70-71 35.752260326560226 36.5 35.0 39.0 32.0 41.0 72-73 35.366306904219385 36.0 35.0 39.0 32.0 41.0 74-75 34.98819371263352 35.5 35.0 38.0 32.0 40.0 76-77 33.91165070884246 35.0 33.5 36.5 30.0 39.0 78-79 34.27582194801778 35.0 34.5 37.0 31.0 39.0 80-81 34.09267151503095 35.0 35.0 36.0 31.0 38.5 82-83 33.83728275254531 35.0 35.0 36.0 31.0 37.0 84-85 33.596486674989734 35.0 34.5 36.0 31.0 37.0 86-87 33.401777968886435 35.0 34.0 35.0 31.0 36.5 88-89 33.21215450675028 35.0 34.0 35.0 31.0 36.0 90-91 33.054996163483096 35.0 34.0 35.0 31.0 36.0 92-93 32.89492328096499 35.0 34.0 35.0 30.0 36.0 94-95 32.785032592844125 35.0 34.0 35.0 30.0 36.0 96-97 32.65784830951131 35.0 34.0 35.0 30.0 35.0 98-99 32.5090321150196 35.0 34.0 35.0 30.0 35.0 100-101 32.119436232496376 35.0 33.5 35.0 28.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39918553910914056 1101 2 0.32206295830637544 1101 3 0.27376167255815886 1101 4 0.15549989406783027 1101 5 0.14421658216446787 1101 6 0.13104946920172011 1101 7 0.17330359114389893 1101 8 0.17245788229062953 1101 9 0.19930346052011316 1101 10-11 0.17131116270457625 1101 12-13 0.20263664829270311 1101 14-15 0.3282116152573664 1101 16-17 0.35073475380090713 1101 18-19 0.3594016227939889 1101 20-21 0.3343691763760859 1101 22-23 0.33580387223882724 1101 24-25 0.38036220149593447 1101 26-27 0.35164216414174376 1101 28-29 0.35604188744415666 1101 30-31 0.3877394416450599 1101 32-33 0.3744780552334035 1101 34-35 0.4226520121407731 1101 36-37 0.42402839183162655 1101 38-39 0.37281643430411293 1101 40-41 0.4013775361045404 1101 42-43 0.43994400982303006 1101 44-45 0.40196557653481335 1101 46-47 0.3761099487419912 1101 48-49 0.3716503596603715 1101 50-51 0.3827893191518825 1101 52-53 0.3306503399744969 1101 54-55 0.35877836468250734 1101 56-57 0.36587610107611823 1101 58-59 0.4267173234999788 1101 60-61 0.4486971192904008 1101 62-63 0.4268274393203768 1101 64-65 0.4122537189313533 1101 66-67 0.38259925554095275 1101 68-69 0.35806765151128417 1101 70-71 0.3314264154590134 1101 72-73 0.3199491048395089 1101 74-75 0.3159957355654299 1101 76-77 0.26575227876763563 1101 78-79 0.3653650024845163 1101 80-81 0.3788181128763384 1101 82-83 0.34282297665064476 1101 84-85 0.3598729462109631 1101 86-87 0.3754128564266068 1101 88-89 0.3759433132803949 1101 90-91 0.3706686707472926 1101 92-93 0.4141930583789417 1101 94-95 0.39296318832978017 1101 96-97 0.36809892010803935 1101 98-99 0.40429378567739604 1101 100-101 0.4677599237229444 1102 1 0.31526721227646703 1102 2 0.2601446122905884 1102 3 0.20416772048194076 1102 4 0.12331557415605232 1102 5 0.12940358991458822 1102 6 0.13196916892253796 1102 7 0.11853234396660639 1102 8 0.13284133294369838 1102 9 0.16784356792854993 1102 10-11 0.11876050898697343 1102 12-13 0.1515745516749405 1102 14-15 0.21057920939274055 1102 16-17 0.25625726241307234 1102 18-19 0.28180832423908697 1102 20-21 0.25968484577730067 1102 22-23 0.2253601150185176 1102 24-25 0.257593903674767 1102 26-27 0.2898694867409759 1102 28-29 0.2990500482152072 1102 30-31 0.300274955964575 1102 32-33 0.2544575472101016 1102 34-35 0.28377471844829927 1102 36-37 0.26137649187216994 1102 38-39 0.25954035992334923 1102 40-41 0.2794886920419586 1102 42-43 0.30045307186538395 1102 44-45 0.25830522560195845 1102 46-47 0.24754382320532642 1102 48-49 0.22508820627895432 1102 50-51 0.1791887570787125 1102 52-53 0.24198272770301799 1102 54-55 0.22988664434099348 1102 56-57 0.26360390328984096 1102 58-59 0.2749307815391049 1102 60-61 0.26777380592345423 1102 62-63 0.24690297151398255 1102 64-65 0.2749579354032221 1102 66-67 0.31233458577014517 1102 68-69 0.20449978465349972 1102 70-71 0.17261494452143467 1102 72-73 0.27901277855041684 1102 74-75 0.245721054641443 1102 76-77 0.22520832632167043 1102 78-79 0.28479186658362465 1102 80-81 0.24849448222876447 1102 82-83 0.27921022535781503 1102 84-85 0.27933198962018224 1102 86-87 0.27187086143002404 1102 88-89 0.2495602893643536 1102 90-91 0.276359741271186 1102 92-93 0.3012108993465574 1102 94-95 0.31309181877873726 1102 96-97 0.3237429077983336 1102 98-99 0.35932118348615205 1102 100-101 0.3818516753251586 1103 1 0.2191328997554649 1103 2 0.19732387278443753 1103 3 0.15504643054867984 1103 4 0.05318468559192269 1103 5 0.09156354909126918 1103 6 0.07144602317152504 1103 7 0.07185201279119724 1103 8 0.0944644750522059 1103 9 0.09178506041017442 1103 10-11 0.10476674709280331 1103 12-13 0.10341317589198695 1103 14-15 0.13690827690746943 1103 16-17 0.12920745504044362 1103 18-19 0.1379303793258302 1103 20-21 0.1593755969065569 1103 22-23 0.1756910941119969 1103 24-25 0.18230073233201693 1103 26-27 0.17129826647492763 1103 28-29 0.2042622446913711 1103 30-31 0.20134680061992327 1103 32-33 0.19046017344652455 1103 34-35 0.14082992830607566 1103 36-37 0.15426013328105626 1103 38-39 0.17023171348473198 1103 40-41 0.1577726217422466 1103 42-43 0.12121482113583681 1103 44-45 0.1852281942885341 1103 46-47 0.16948829119557018 1103 48-49 0.16263370669874888 1103 50-51 0.07944231076444197 1103 52-53 0.17521023995789875 1103 54-55 0.1437092095262713 1103 56-57 0.1285626572845402 1103 58-59 0.11004892756870532 1103 60-61 0.1939920288768917 1103 62-63 0.19268040115430551 1103 64-65 0.16727850078390816 1103 66-67 0.18012242753462715 1103 68-69 0.1477052217254382 1103 70-71 0.14654146414200397 1103 72-73 0.12205189147619677 1103 74-75 0.1283015032735193 1103 76-77 0.09122550556627829 1103 78-79 0.1882513702426465 1103 80-81 0.16239592528577163 1103 82-83 0.1197079144082025 1103 84-85 0.14157652305073753 1103 86-87 0.19017177134197283 1103 88-89 0.20829528742564918 1103 90-91 0.22922938455851494 1103 92-93 0.22629989850922527 1103 94-95 0.1474091419874881 1103 96-97 0.19145783473655342 1103 98-99 0.23075322785983587 1103 100-101 0.19269439832745405 1104 1 0.21212960100688605 1104 2 0.15553138769972463 1104 3 0.11034529771255563 1104 4 0.05974208506699341 1104 5 0.051674818518975485 1104 6 0.036839992998281446 1104 7 0.06074806021241841 1104 8 0.05368039422438642 1104 9 0.04272236558573894 1104 10-11 0.07343307182920711 1104 12-13 0.0664543703821252 1104 14-15 0.07465046569191003 1104 16-17 0.08482215686598238 1104 18-19 0.08349593465133864 1104 20-21 0.09092482677388602 1104 22-23 0.09763727439288772 1104 24-25 0.10560977757251067 1104 26-27 0.046816964631339886 1104 28-29 0.12387262827491696 1104 30-31 0.1456186969852169 1104 32-33 0.13456406896158057 1104 34-35 0.09832457482728074 1104 36-37 0.1165687554361412 1104 38-39 0.11089889504902573 1104 40-41 0.12730911021861857 1104 42-43 0.16672784295288068 1104 44-45 0.14386760897162532 1104 46-47 0.12342718243278483 1104 48-49 0.12553658897898856 1104 50-51 0.11733448608708841 1104 52-53 0.06481984848230837 1104 54-55 0.06366105122664578 1104 56-57 0.08011856525518368 1104 58-59 0.09893339948167323 1104 60-61 0.13971083955252084 1104 62-63 0.15995996779547994 1104 64-65 0.14394596156324724 1104 66-67 0.16399513356545015 1104 68-69 0.15428400314452517 1104 70-71 0.11281251026742467 1104 72-73 0.11540812037944903 1104 74-75 0.0863098444599757 1104 76-77 0.04731503768535106 1104 78-79 0.1325154792012313 1104 80-81 0.1232282070414712 1104 82-83 0.09347513295249854 1104 84-85 0.13082898819899214 1104 86-87 0.1653067018719625 1104 88-89 0.16459685537998325 1104 90-91 0.17536652856053792 1104 92-93 0.21436056103171808 1104 94-95 0.160512290829935 1104 96-97 0.12860444482649314 1104 98-99 0.19795751494445568 1104 100-101 0.24993445342253295 1105 1 0.10734100767515997 1105 2 0.10114948840119098 1105 3 0.06085454102001364 1105 4 0.049925451511519725 1105 5 0.00677296791479165 1105 6 0.009748045025389729 1105 7 0.04184443533874571 1105 8 0.0104191269837699 1105 9 0.009335661434747067 1105 10-11 0.004967971058690068 1105 12-13 -0.007267737721868173 1105 14-15 -0.01603768897622615 1105 16-17 0.03234508991840812 1105 18-19 0.01224000870501385 1105 20-21 0.056948492864904665 1105 22-23 0.05746542545763589 1105 24-25 0.048894701627610004 1105 26-27 0.019613324463172432 1105 28-29 0.048433876039872814 1105 30-31 0.033320733818960946 1105 32-33 0.03307497777465329 1105 34-35 0.0376653371991722 1105 36-37 0.03444272573577223 1105 38-39 0.03148664076534402 1105 40-41 -0.029138128808291697 1105 42-43 -0.120213808876251 1105 44-45 -0.02690049117610016 1105 46-47 0.0031672013182415526 1105 48-49 -0.004828384395629826 1105 50-51 0.0018894406049838608 1105 52-53 -0.005809180282696502 1105 54-55 -0.0018592155984293868 1105 56-57 -0.057622626236572216 1105 58-59 -0.024294652260422822 1105 60-61 0.034253293787458006 1105 62-63 0.07079807174608987 1105 64-65 0.028008879715763157 1105 66-67 0.10701389955159613 1105 68-69 0.08250427798403592 1105 70-71 0.05251275639609787 1105 72-73 0.04157026076104131 1105 74-75 0.04866266935368202 1105 76-77 0.02005172947520606 1105 78-79 0.03304184206321992 1105 80-81 0.031138163864802948 1105 82-83 0.03708366902085203 1105 84-85 0.02352719989540475 1105 86-87 0.05410334826769514 1105 88-89 0.08987669674068144 1105 90-91 0.023350229205249207 1105 92-93 0.04048223762278269 1105 94-95 0.0288902309352963 1105 96-97 0.040934047322600975 1105 98-99 0.1159969115314965 1105 100-101 0.13579055542449936 1106 1 0.012052806388915371 1106 2 0.02524688030486999 1106 3 -0.00541819788620046 1106 4 -0.0032316414088242595 1106 5 -0.03984403376043133 1106 6 -0.02429221180266694 1106 7 -0.05955988537136392 1106 8 -0.063794710378545 1106 9 -0.10254309750677493 1106 10-11 -0.07987497154850587 1106 12-13 -0.05654378601202836 1106 14-15 -0.062820137763687 1106 16-17 -0.0632342325601627 1106 18-19 -0.046272869694135466 1106 20-21 -0.08428378119382529 1106 22-23 -0.1020238040616448 1106 24-25 -0.12732234767188544 1106 26-27 -0.12455770162165436 1106 28-29 -0.18650826026068756 1106 30-31 -0.12981413428996547 1106 32-33 -0.11428184816202958 1106 34-35 -0.13112501616463845 1106 36-37 -0.1736757200133141 1106 38-39 -0.17415456574499188 1106 40-41 -0.2229429300516088 1106 42-43 -0.1914147420624417 1106 44-45 -0.2539295362229339 1106 46-47 -0.2172737873565822 1106 48-49 -0.19444593128537946 1106 50-51 -0.2052574468558177 1106 52-53 -0.20279707485052256 1106 54-55 -0.1855415221306984 1106 56-57 -0.1495649527559877 1106 58-59 -0.16532787932448656 1106 60-61 -0.15801900232749233 1106 62-63 -0.12301166109703132 1106 64-65 -0.1112511232437896 1106 66-67 -0.10765710226539227 1106 68-69 -0.08643234527529842 1106 70-71 -0.06838730797677073 1106 72-73 -0.04367190070253457 1106 74-75 -0.03950596188325761 1106 76-77 -0.10322342545848073 1106 78-79 -0.017256303290849928 1106 80-81 0.016909375046203934 1106 82-83 -0.050067438605488235 1106 84-85 -0.026510937368023235 1106 86-87 -0.03872271077680267 1106 88-89 -0.04323556868100553 1106 90-91 -0.08603869500464612 1106 92-93 -0.05357695216093816 1106 94-95 -0.02551882062854105 1106 96-97 0.004235236377027718 1106 98-99 -0.03481286024762653 1106 100-101 -0.00758185074559492 1107 1 -0.12521524220819913 1107 2 -0.10711746940376088 1107 3 -0.11783398680383073 1107 4 -0.08302440640807163 1107 5 -0.11590178730389056 1107 6 -0.08654067702919832 1107 7 -0.11023365555102771 1107 8 -0.11350214495337951 1107 9 -0.16978755023864522 1107 10-11 -0.1578839857814458 1107 12-13 -0.22191880497779692 1107 14-15 -0.3558117764170561 1107 16-17 -0.3328228204004233 1107 18-19 -0.32543485334829114 1107 20-21 -0.28639368307479174 1107 22-23 -0.31780659421229984 1107 24-25 -0.3515771508809422 1107 26-27 -0.4267369161327039 1107 28-29 -0.3901212902961291 1107 30-31 -0.31382770288410455 1107 32-33 -0.35363854499471614 1107 34-35 -0.29620788784406926 1107 36-37 -0.2896521107553056 1107 38-39 -0.385398302140608 1107 40-41 -0.33551088578617794 1107 42-43 -0.26960860205274173 1107 44-45 -0.28338783088570096 1107 46-47 -0.2965557314374436 1107 48-49 -0.3245949822388994 1107 50-51 -0.2940444352809237 1107 52-53 -0.2895500179713366 1107 54-55 -0.270808592631127 1107 56-57 -0.2931863276322062 1107 58-59 -0.2316961384853684 1107 60-61 -0.24570313561395096 1107 62-63 -0.27709032549810786 1107 64-65 -0.26527934438873046 1107 66-67 -0.2481879525926658 1107 68-69 -0.1882833189411528 1107 70-71 -0.276171169744849 1107 72-73 -0.23026488320438432 1107 74-75 -0.2721690815189248 1107 76-77 -0.3522234800741302 1107 78-79 -0.19716608048151585 1107 80-81 -0.1993223663795689 1107 82-83 -0.18976005226905812 1107 84-85 -0.1765550772567579 1107 86-87 -0.21845275636470518 1107 88-89 -0.19045510432145107 1107 90-91 -0.20380273524480685 1107 92-93 -0.13813810294396234 1107 94-95 -0.17112790474391915 1107 96-97 -0.17545805134509607 1107 98-99 -0.20519943787731876 1107 100-101 -0.19306308053330667 1108 1 -0.29823462494348973 1108 2 -0.24875118714452782 1108 3 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2304 96-97 -0.01565348022685953 2304 98-99 -0.03916004124804573 2304 100-101 -0.005438059110467464 2305 1 0.05442742213920582 2305 2 -0.38464919153991417 2305 3 -0.29204586920109676 2305 4 -0.09509895207341401 2305 5 -0.05218395278401289 2305 6 -0.017211582190427066 2305 7 0.007873442249191953 2305 8 -0.00879866494891246 2305 9 0.017096311909952533 2305 10-11 -1.468281351577616E-4 2305 12-13 0.01008337575995455 2305 14-15 0.014193874397740558 2305 16-17 -0.034336297893901246 2305 18-19 -0.0040414977709346545 2305 20-21 -0.008212608706870128 2305 22-23 -0.010018314846533372 2305 24-25 0.013403999546369505 2305 26-27 -0.0030994088974765077 2305 28-29 0.043612147644502386 2305 30-31 0.05024958197038387 2305 32-33 -0.020992017258201656 2305 34-35 -0.013057729383824324 2305 36-37 0.00415876922851055 2305 38-39 -0.0025884287307391673 2305 40-41 0.005425706645205253 2305 42-43 0.02996502125564149 2305 44-45 0.029279172222295813 2305 46-47 -0.00871015389604679 2305 48-49 -0.019318913926277048 2305 50-51 0.03862946909923437 2305 52-53 -0.03298476487195501 2305 54-55 -0.0945311078232578 2305 56-57 -0.009178268968838665 2305 58-59 -0.065273953364958 2305 60-61 -0.004271560831639931 2305 62-63 -0.041089248811424284 2305 64-65 -0.021025031937710992 2305 66-67 -0.0290166121061759 2305 68-69 -0.01227674500700715 2305 70-71 -0.03573202722451185 2305 72-73 0.021972841905849805 2305 74-75 0.027471271422356836 2305 76-77 0.06002059548077199 2305 78-79 -0.19927859376277723 2305 80-81 -0.20119856363974264 2305 82-83 -0.06274505968685418 2305 84-85 -0.03868493234637782 2305 86-87 -0.015187594354713951 2305 88-89 -0.01139963555744572 2305 90-91 -0.04072055850851797 2305 92-93 -0.0324508097799594 2305 94-95 -0.056435366275437104 2305 96-97 -0.06991339064421709 2305 98-99 -0.11799447638378524 2305 100-101 -0.06633624383675141 2306 1 -0.06217450164918148 2306 2 -0.28741358266211847 2306 3 -0.24331325214744481 2306 4 -0.12765429309838794 2306 5 -0.14007740330906415 2306 6 -0.10896606835107292 2306 7 -0.11356466106542484 2306 8 -0.12313536966849625 2306 9 -0.17287440550821032 2306 10-11 -0.1391544527087447 2306 12-13 -0.15105600782737838 2306 14-15 -0.1718550316063272 2306 16-17 -0.17966754912973215 2306 18-19 -0.1447611043400414 2306 20-21 -0.1523684112994772 2306 22-23 -0.1963905101107244 2306 24-25 -0.21985329708528667 2306 26-27 -0.2023433159442689 2306 28-29 -0.24040630854103995 2306 30-31 -0.38468791809244607 2306 32-33 -0.3114653930101312 2306 34-35 -0.28716587862919596 2306 36-37 -0.2768996865419595 2306 38-39 -0.3726285332809809 2306 40-41 -0.3372494767188101 2306 42-43 -0.35829400890660423 2306 44-45 -0.32003471442390463 2306 46-47 -0.2623561729272055 2306 48-49 -0.3873996630385861 2306 50-51 -0.3576209282219551 2306 52-53 -0.3003540333312884 2306 54-55 -0.23968285178382587 2306 56-57 -0.23279319154726608 2306 58-59 -0.23823916971998216 2306 60-61 -0.26606183571284703 2306 62-63 -0.209152233751432 2306 64-65 -0.22651502876131246 2306 66-67 -0.21640671159151736 2306 68-69 -0.18206407165020977 2306 70-71 -0.16154911497354618 2306 72-73 -0.16066068524660437 2306 74-75 -0.15030605903942273 2306 76-77 -0.08862636780366273 2306 78-79 -0.1449239284004591 2306 80-81 -0.19387768490123136 2306 82-83 -0.17283087318590873 2306 84-85 -0.1834222160784904 2306 86-87 -0.18390406314245666 2306 88-89 -0.2252423978139504 2306 90-91 -0.2734641325267475 2306 92-93 -0.6670732352602187 2306 94-95 -0.42790332487672345 2306 96-97 -0.32967809181344876 2306 98-99 -0.30593757524919596 2306 100-101 -0.2810330276329296 2307 1 -0.46375161573121915 2307 2 -0.3740310834827696 2307 3 -0.3116427080227524 2307 4 -0.178554531459433 2307 5 -0.19552519062332152 2307 6 -0.22284557876489686 2307 7 -0.2172002958802537 2307 8 -0.1965547583031224 2307 9 -0.259829095725685 2307 10-11 -0.32011120166875173 2307 12-13 -0.2928664017565765 2307 14-15 -0.5566824925600642 2307 16-17 -0.5158471797326456 2307 18-19 -0.4562082580139304 2307 20-21 -0.4419470999187922 2307 22-23 -0.3851136303280214 2307 24-25 -0.3728531761567453 2307 26-27 -0.382791034993474 2307 28-29 -0.37347554392284366 2307 30-31 -0.471743669231806 2307 32-33 -0.45891618444280624 2307 34-35 -0.41410006351527784 2307 36-37 -0.43048500815311286 2307 38-39 -0.4631065638128504 2307 40-41 -0.4297311710066154 2307 42-43 -0.41237676135194334 2307 44-45 -0.5401292520362588 2307 46-47 -0.4622284654672413 2307 48-49 -0.3524324380377024 2307 50-51 -0.5159143187848159 2307 52-53 -0.4192936256060307 2307 54-55 -0.43062561391859333 2307 56-57 -0.4912698597927303 2307 58-59 -0.4467710498071753 2307 60-61 -0.38521092812845126 2307 62-63 -0.469499755270796 2307 64-65 -0.4376470365126437 2307 66-67 -0.4369743777914863 2307 68-69 -0.4422837193215017 2307 70-71 -0.43911490888715576 2307 72-73 -0.4786463363236635 2307 74-75 -0.4553552072059901 2307 76-77 -0.4876313643027572 2307 78-79 -0.4157327061267395 2307 80-81 -0.40990546541447515 2307 82-83 -0.406253141809124 2307 84-85 -0.40250427756804896 2307 86-87 -0.39174999297731716 2307 88-89 -0.450234980003934 2307 90-91 -0.45066622626998054 2307 92-93 -0.5123006852205378 2307 94-95 -0.4425913908881469 2307 96-97 -0.47505166165917956 2307 98-99 -0.5225595577792461 2307 100-101 -0.5834347769586969 2308 1 -0.6558334902644845 2308 2 -0.5733162909521994 2308 3 -0.5456404102590895 2308 4 -0.3275987333987658 2308 5 -0.3762119329521525 2308 6 -0.3837554629195452 2308 7 -0.378837694466192 2308 8 -0.37227645039621393 2308 9 -0.38230045705335414 2308 10-11 -0.48035231569832604 2308 12-13 -0.4827833115057203 2308 14-15 -0.6996618251647462 2308 16-17 -0.8570931135149777 2308 18-19 -0.7009974805476986 2308 20-21 -0.709824082920747 2308 22-23 -0.920031864104061 2308 24-25 -0.8597666315227102 2308 26-27 -0.7759629539088522 2308 28-29 -0.9096691646306283 2308 30-31 -0.8957887129315552 2308 32-33 -0.8184639975180303 2308 34-35 -0.8713860696868352 2308 36-37 -0.8389321060783601 2308 38-39 -0.714674752862301 2308 40-41 -0.6818472818884018 2308 42-43 -0.6549088321227359 2308 44-45 -0.6637711086950517 2308 46-47 -0.6653483326188123 2308 48-49 -0.6924522503156609 2308 50-51 -0.6386746184956067 2308 52-53 -0.6731119901742346 2308 54-55 -0.8172384045334695 2308 56-57 -0.6887944332519211 2308 58-59 -0.7684441137815483 2308 60-61 -0.9166463004178027 2308 62-63 -0.8204140542252745 2308 64-65 -0.8169226930837823 2308 66-67 -0.9692243552261033 2308 68-69 -0.7903960078584404 2308 70-71 -0.6176589287217951 2308 72-73 -0.6829732235715582 2308 74-75 -0.6474238186191954 2308 76-77 -0.7187603724491325 2308 78-79 -0.6359240742108128 2308 80-81 -0.6040768619660284 2308 82-83 -0.5424416817928659 2308 84-85 -0.5746068800915154 2308 86-87 -0.5527181426888603 2308 88-89 -0.6235198224255925 2308 90-91 -0.6941444023318226 2308 92-93 -0.6385200830190456 2308 94-95 -0.6476374056356988 2308 96-97 -0.6519057329175908 2308 98-99 -0.7243254857908568 2308 100-101 -0.8117633492181433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 8.0 11 82.0 12 289.0 13 751.0 14 2013.0 15 4197.0 16 7222.0 17 12235.0 18 22731.0 19 49951.0 20 60955.0 21 62012.0 22 60429.0 23 61661.0 24 64266.0 25 71102.0 26 80751.0 27 95386.0 28 116513.0 29 142172.0 30 174560.0 31 218441.0 32 279290.0 33 370011.0 34 538153.0 35 981258.0 36 1769428.0 37 4145878.0 38 5889868.0 39 1526031.0 40 1874.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.121696296167606 28.10370797344114 27.662936472785592 23.111659257605663 2 23.081560101842303 27.68606452606196 28.416376959767675 20.815998412328064 3 22.429393870782018 26.44407174554321 27.147167455961558 23.979366927713215 4 24.072304124767207 25.685058135546708 27.334978107065695 22.907659632620398 5 24.313925120552373 26.673692059471847 26.320915334835384 22.691467485140397 6 24.83933209744622 26.30002240397375 25.401680167152918 23.458965331427112 7 25.026948577432208 27.18381639688343 25.574487034073982 22.214747991610377 8 24.86556128498152 26.41264907179373 25.849206384144985 22.872583259079768 9 23.95386351946558 26.00996649636236 26.22717082072193 23.808999163450135 10-11 23.869836719886912 26.33648091515771 25.872264749054676 23.921417615900705 12-13 23.92401138450252 26.490286634036742 25.885462628970085 23.700239352490655 14-15 23.373614877000044 26.824183774930372 26.149286374540896 23.652914973528688 16-17 23.457812623814387 26.918398689878707 26.265069214695842 23.358719471611064 18-19 22.802848957358563 27.14575456595484 27.014007778212317 23.03738869847428 20-21 22.24875811430167 26.767052452069116 27.43722038906767 23.54696904456154 22-23 22.238261085178053 26.5084073201861 28.401471713823085 22.851859880812764 24-25 22.23989111407002 26.286333730687577 29.10121515679391 22.372559998448498 26-27 22.2586862704148 26.310018945989285 29.16952982338702 22.2617649602089 28-29 22.26519305628047 26.414376639682757 29.140745628199262 22.179684675837514 30-31 22.25734520352084 26.394685459841266 29.09507102141663 22.252898315221262 32-33 22.279211099330748 26.433779957283726 29.05770706810273 22.2293018752828 34-35 22.285555719087245 26.52311029977183 28.998303223209614 22.19303075793131 36-37 22.315546233697404 26.51549558964552 28.87308536943432 22.295872807222757 38-39 22.331620153129077 26.59960787600853 28.747857087063792 22.320914883798597 40-41 22.395679851945772 26.724174368727383 28.593353651784092 22.28679212754275 42-43 22.38982408249473 26.72408905653041 28.531698281195183 22.354388579779673 44-45 22.356762400920715 26.800756571366296 28.533081674322847 22.309399353390145 46-47 22.35723447623645 26.928217330399356 28.468625752944625 22.245922440419566 48-49 22.377393983955944 26.88973191579724 28.42595258373714 22.306921516509675 50-51 22.351234734016572 26.935230559900784 28.436363102027666 22.277171604054978 52-53 22.347025950059493 27.020165221840404 28.404870578683948 22.227938249416155 54-55 22.335016955400057 27.012446135773843 28.326803452878792 22.32573345594731 56-57 22.3325641231727 27.11882259384948 28.25203579721527 22.296577485762555 58-59 22.385321832309106 27.22802200842266 28.161069232102808 22.225586927165427 60-61 22.37264833197867 27.24180267219538 28.017770017482974 22.36777897834298 62-63 22.40467573192759 27.343811999844377 27.92142820513949 22.33008406308854 64-65 22.433581974212466 27.467599011464817 27.784508752719738 22.31431026160298 66-67 22.42265268285193 27.530781764960103 27.634689770264014 22.41187578192395 68-69 22.418445234130076 27.66470119102874 27.501008060797993 22.41584551404319 70-71 22.458367764793906 27.757718682992135 27.41814002977686 22.365773522437095 72-73 22.449001809570028 27.806029298401057 27.28416424551852 22.460804646510386 74-75 22.44601838077689 27.89932465642382 27.23816054689968 22.416496415899612 76-77 22.456212010362226 28.025988014647417 27.134960086303483 22.382839888686874 78-79 22.461286516365313 28.074249362771734 27.052173655425456 22.412290465437497 80-81 22.439980135063955 28.168434097872407 27.002567830915797 22.38901793614784 82-83 22.435598801648908 28.295118972200783 26.951075857356503 22.318206368793803 84-85 22.39684385953244 28.354388269788583 26.874104896999423 22.374662973679555 86-87 22.40870023756779 28.498898659676026 26.78291846321828 22.309482639537908 88-89 22.399841534457615 28.635873508729986 26.680008565801465 22.28427639101093 90-91 22.38588019414617 28.650553582056578 26.624259501578774 22.33930672221848 92-93 22.362506192811775 28.768218318943305 26.558855099879956 22.310420388364964 94-95 22.359551551088487 28.903885413416962 26.499015897696193 22.23754713779836 96-97 22.280984275177858 28.972120627098718 26.435347759911927 22.311547337811493 98-99 22.277649632442373 29.08624500006633 26.40733888563907 22.228766481852226 100-101 22.305294339651375 29.21091763762994 26.295261515207187 22.1885265075115 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 101395.0 1 58711.5 2 12071.0 3 7289.5 4 6281.0 5 6140.0 6 6119.5 7 5895.0 8 5754.5 9 5716.0 10 6058.0 11 6652.0 12 7420.5 13 10457.0 14 15600.5 15 20322.0 16 53831.5 17 55986.0 18 25663.0 19 25057.5 20 26902.5 21 30374.0 22 35266.0 23 42369.0 24 50421.0 25 60467.0 26 74464.0 27 93756.0 28 116525.0 29 143823.5 30 177620.5 31 210812.5 32 244012.0 33 283745.0 34 324327.0 35 366414.0 36 410275.5 37 449624.0 38 482620.0 39 514368.5 40 535969.5 41 538810.0 42 544077.5 43 561905.0 44 578318.0 45 591918.0 46 606329.5 47 624497.0 48 637172.5 49 628646.5 50 606814.5 51 582863.5 52 569128.0 53 558370.0 54 533708.5 55 500139.5 56 458833.0 57 415594.5 58 380909.0 59 346489.0 60 302505.5 61 258118.5 62 215010.0 63 168154.5 64 128362.0 65 95789.5 66 69470.5 67 51645.5 68 39452.5 69 31265.5 70 25615.0 71 20893.0 72 17509.0 73 14115.5 74 10915.0 75 9108.0 76 7784.5 77 5751.0 78 3433.5 79 1836.5 80 1019.5 81 655.0 82 393.0 83 164.0 84 73.5 85 36.0 86 16.0 87 7.0 88 4.5 89 5.0 90 3.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012909352903515733 2 0.0 3 0.0 4 0.00670453489505172 5 1.1303120053769536E-4 6 0.0 7 0.0038847038921639514 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.878188654784748E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0024956099276612215 28-29 3.2422107522654724E-4 30-31 6.5439116100771E-5 32-33 0.0 34-35 0.0 36-37 2.468839380165451E-4 38-39 2.3201141163000627E-4 40-41 8.923515831923319E-6 42-43 6.246461082346324E-4 44-45 0.0 46-47 8.92351583192332E-5 48-49 6.930597296127111E-4 50-51 0.0012463177111919567 52-53 3.420681068903939E-4 54-55 7.376773087723276E-4 56-57 0.0029804542878623884 58-59 0.00621076701901863 60-61 0.0018174227244350492 62-63 0.0 64-65 0.0 66-67 0.002489660917106606 68-69 9.518416887384873E-5 70-71 9.369691623519486E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.175639182515525E-4 84-85 0.0 86-87 0.0 88-89 0.004857367117843593 90-91 0.009914026089266807 92-93 0.004256517051827423 94-95 0.0017252130608385082 96-97 6.841362137807878E-5 98-99 2.6770547495769955E-4 100-101 5.592069921338613E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6809518E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.37464452688058 #Duplication Level Percentage of deduplicated Percentage of total 1 76.357815617325 34.64708740485213 2 11.765126744364315 10.676768876784532 3 4.31956987950788 5.879968433750713 4 2.085176229238235 3.784565207103448 5 1.153046444615121 2.61595362736973 6 0.7224592182674244 1.9668798128431448 7 0.49387458222592184 1.5686568526554003 8 0.36561930637579326 1.327187684717301 9 0.2668622261160901 1.0897900782902652 >10 2.1518655107033715 20.36812840136179 >50 0.23196688708314123 7.348098438289856 >100 0.08625168952211779 5.641290206674887 >500 9.244899011466428E-5 0.02942909719891721 >1k 1.0410090362528921E-4 0.11047236918788608 >5k 3.902648350570072E-5 0.14413235782033987 >10k+ 1.3008827835233572E-4 2.8015911510996916 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136608 0.8126824338449203 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106172 0.6316183486046417 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78357 0.4661466200280103 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50983 0.3032984051059644 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29849 0.17757201604471942 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 18991 0.11297765944270383 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 17538 0.10433374710684745 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.866856860500105E-4 0.0 0.0 5.949010554615546E-6 0.0 2 3.866856860500105E-4 0.0 0.0 5.949010554615546E-6 5.949010554615546E-6 3 3.866856860500105E-4 0.0 0.0 5.949010554615546E-6 5.949010554615546E-6 4 3.9263469660462603E-4 0.0 0.0 5.949010554615546E-6 2.3796042218462183E-5 5 3.9263469660462603E-4 0.0 0.0 5.949010554615546E-6 2.9745052773077728E-5 6 3.985837071592416E-4 0.0 0.0 5.949010554615546E-6 4.164307388230882E-5 7 4.0453271771385713E-4 0.0 0.0 5.949010554615546E-6 4.164307388230882E-5 8 4.0453271771385713E-4 5.949010554615546E-6 0.0 5.949010554615546E-6 4.7592084436924366E-5 9 4.0453271771385713E-4 5.949010554615546E-6 0.0 5.949010554615546E-6 4.7592084436924366E-5 10-11 4.075072229911649E-4 5.949010554615546E-6 0.0 5.949010554615546E-6 6.841362137807878E-5 12-13 0.0013771959433934988 5.949010554615546E-6 0.0 5.949010554615546E-6 8.626065304192542E-5 14-15 0.0016151563655781207 5.949010554615546E-6 0.0 8.923515831923319E-6 9.220966359654097E-5 16-17 0.0017876776716619714 5.949010554615546E-6 0.0 1.1898021109231091E-5 1.0708218998307982E-4 18-19 0.0021208222627204423 5.949010554615546E-6 0.0 1.1898021109231091E-5 1.0708218998307982E-4 20-21 0.0023617571901823715 5.949010554615546E-6 0.0 1.1898021109231091E-5 1.1898021109231091E-4 22-23 0.0025729470648712238 5.949010554615546E-6 0.0 1.1898021109231091E-5 1.3385273747884978E-4 24-25 0.0027454683709550744 5.949010554615546E-6 0.0 1.1898021109231091E-5 1.4872526386538863E-4 26-27 0.0028228055081650763 8.923515831923319E-6 0.0 1.1898021109231091E-5 1.5467427442000418E-4 28-29 0.002950709235089311 1.1898021109231091E-5 0.0 1.4872526386538866E-5 1.6062328497461973E-4 30-31 0.0030577914250723903 1.7847031663846638E-5 0.0 1.7847031663846638E-5 1.635977902519275E-4 32-33 0.0031767716361647013 2.3796042218462183E-5 0.0 2.082153694115441E-5 1.8441932719308194E-4 34-35 0.0032927773419797043 2.3796042218462183E-5 0.0 2.3796042218462183E-5 2.0524086413423631E-4 36-37 0.0034355535952904775 2.974505277307773E-5 0.0 2.3796042218462183E-5 2.2308789579808297E-4 38-39 0.003581304353878559 3.5694063327693276E-5 0.0 3.27195580503855E-5 2.3796042218462185E-4 40-41 0.003756800165239717 3.5694063327693276E-5 0.0 3.5694063327693276E-5 2.4390943273923737E-4 42-43 0.003947168502987415 3.5694063327693276E-5 0.0 3.8668568605001045E-5 2.587819591257762E-4 44-45 0.004119689809071266 3.5694063327693276E-5 0.0 4.164307388230882E-5 2.7960349606693066E-4 46-47 0.004384420778751657 3.5694063327693276E-5 0.0 5.0566589714232135E-5 2.88527011898854E-4 48-49 0.0047413614120285896 3.5694063327693276E-5 0.0 5.9490105546155455E-5 3.033995382853928E-4 50-51 0.005119123582246678 3.8668568605001045E-5 0.0 6.246461082346322E-5 3.2124656994923946E-4 52-53 0.005595044426615921 4.164307388230882E-5 0.0 6.5439116100771E-5 3.27195580503855E-4 54-55 0.006032296702380164 4.164307388230882E-5 0.0 7.138812665538655E-5 3.3314459105847057E-4 56-57 0.006529039083690562 4.164307388230882E-5 0.0 7.733713721000209E-5 3.3611909633577834E-4 58-59 0.007049577507219422 4.164307388230882E-5 0.0 8.031164248730987E-5 3.599151385542405E-4 60-61 0.0075225238463113575 4.164307388230882E-5 0.0 8.328614776461764E-5 3.718131596634716E-4 62-63 0.008191787533705606 4.164307388230882E-5 0.0 8.328614776461764E-5 3.837111807727027E-4 64-65 0.008985980442746781 4.164307388230882E-5 0.0 8.626065304192542E-5 3.8966019132731825E-4 66-67 0.009893204552325653 4.164307388230882E-5 0.0 9.220966359654097E-5 4.2237974937770373E-4 68-69 0.011918842646172247 4.7592084436924366E-5 0.0 1.0708218998307982E-4 4.491502968734737E-4 70-71 0.012730882586877268 4.7592084436924366E-5 0.0 1.1303120053769537E-4 4.5509930742808927E-4 72-73 0.013813602507817296 4.7592084436924366E-5 0.0 1.1600570581500315E-4 4.669973285373203E-4 74-75 0.014848730344320403 4.7592084436924366E-5 0.0 1.2492922164692645E-4 4.7592084436924364E-4 76-77 0.016217002771881976 4.7592084436924366E-5 0.0 1.30878232201542E-4 4.7592084436924364E-4 78-79 0.017850006169123946 4.7592084436924366E-5 0.0 1.30878232201542E-4 4.7592084436924364E-4 80-81 0.019831026683810923 4.7592084436924366E-5 0.0 1.30878232201542E-4 4.8484436020116697E-4 82-83 0.02242776979090061 4.7592084436924366E-5 0.0 1.30878232201542E-4 5.056658971423213E-4 84-85 0.026074513260879936 4.7592084436924366E-5 0.0 1.3682724275615755E-4 5.205384235288603E-4 86-87 0.031083580147866226 4.7592084436924366E-5 0.0 1.3980174803346533E-4 5.443344657473225E-4 88-89 0.03737168430409486 5.0566589714232135E-5 0.0 1.427762533107731E-4 5.562324868565536E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6285 0.0 84.28011 1 TCGACGG 7910 0.0 67.679794 2 ATCTCGA 23245 0.0 63.396862 1 TCTCGAG 28675 0.0 51.138126 2 CGACGGC 12670 0.0 42.21564 3 CTCGAGG 37860 0.0 38.618877 3 TCGAGGG 38725 0.0 37.79304 4 CGAGGGC 40940 0.0 35.82793 5 TATCTCG 6510 0.0 33.05771 1 GGGCGCG 44540 0.0 32.941284 8 AGGGCGC 44810 0.0 32.797264 7 ACGGCGC 16725 0.0 32.177776 5 GACGGCG 17190 0.0 31.142944 4 GAGGGCG 48030 0.0 30.657827 6 CGGCGCG 17765 0.0 30.374235 6 ATATCTC 7070 0.0 30.103302 1 GGCGCGC 63320 0.0 23.418812 9 CGCGCCG 61675 0.0 22.595596 9 GCGCGCC 63820 0.0 21.918022 8 GGATCCT 53040 0.0 19.114897 16-17 >>END_MODULE